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The map label for this gene is gatB

Identifier: 118581492

GI number: 118581492

Start: 3378162

End: 3379601

Strand: Direct

Name: gatB

Synonym: Ppro_3089

Alternate gene names: 118581492

Gene position: 3378162-3379601 (Clockwise)

Preceding gene: 118581491

Following gene: 118581493

Centisome position: 84.29

GC content: 61.11

Gene sequence:

>1440_bases
ATGAAATACCAAGCCGTTATCGGACTTGAGGTTCACGTCCAGCTCAAGACCGACTCCAAGATATTCTGCGGATGCTCCAC
CTCCTTCGGATCCGACCCCAACTCCCAAACCTGCCCGGTCTGTCTGGGGCTGCCCGGGGCACTGCCGGTGCTGAACAAAA
AAGTGGTGGAATTCGCCATCAAGGCCGGTCTGGCCACCAACTGCTCCATCACCCAGCGCAGCATCTTCGCCCGCAAGAAC
TACTTCTATCCGGACCTGCCCAAGGGATACCAGATCAGCCAGTTCGAGGATCCCGTCTGTCAGTGGGGCTGGCTGGATAT
CGAGCTGAACGGCGAAACCAAGCGCATCGGCATCACCCGCATCCACATGGAAGAAGACGCCGGCAAGCTGGTGCACGCTG
ACGTCCCCGAGCTGGAGGACGGGTCGGGCGTGGACCTGAACCGGGCCTGCACACCGCTTCTGGAGGTGGTCTCCGAACCG
GACCTGCGCTCATCCGACGAGGCGGTCAGCTATCTCAAGCAGCTGCACCAGATCGTGACCTGGCTCGGTATCTGCGACGG
CAACATGGAGGAGGGGAGCTTCCGCTGCGACGCCAATGTCTCGGTCATGGAGCAGGGGGCCGAGAAATTCGGCACCCGCG
CCGAGATCAAGAACATCAACTCCTTCAGGTTCGTCAAGCAGGCCATCGAATACGAAATCCAGCGCCAGATCGACCTGATC
GAGGATGGCGGCACGGTTGTCCAGGAAACACGCCTGTTCGATCCCGACAACGGCGTGACCCGCTCCATGCGCAGCAAGGA
GGAGGCCCACGACTACCGCTACTTCCCCGATCCCGACCTGGTGCCGCTGGTAATCGACGACGACTGGATCCAGCGGGTGC
GCCAGGGCCTGCCCGAACTGCCCGAGCACAAGAAGCAGCGCTTCATGAGCGAGTTCAGCATCCCCGCCTATGACGCCGAA
GTGCTCACCGCCAGCCGTCCCCTGGCCGAATACTATGAGGAGTGCGTGGCCTGCCACCACGACGGCAAGGCGGTTTCCAA
CTGGGTCATGGGCGAGGTCACCCGCGGACTGAACGACAGCGGACTCTCCATAGAAAACTGCCCGGTCACCCCCCGGCTAC
TGGGGGGACTGCTCAAGCTGATGGATAACGGCACCATCTCCGGCAAGATCGCCAAGACGGTCTTCGACGAGATGTGGAAG
AGCGGCAAAGAGCCGGCCGTCATCGTGGAGGAGCAGGGACTGGTCCAGGTTTCCGACAACGGAGCCATCCTGGCCATCAT
CGACGAAATCATGGCCAAAAACCAGGGACAGGTGGCTGAGTACCGCAGCGGCAAGGACAAGTTGTTCGGGTTCTTTGTCG
GCCAGGTCATGAAGGCCAGCAAGGGCAAGGCCAACCCCTCCCTGGTTAACGAGTTGCTGCTGGAGAAGCTGAAGGGATAG

Upstream 100 bases:

>100_bases
GGCACAGCCTGAGGGCGCCGCTGTAGGGGCGGCCCCTGTGACTGCCACCAAGGGTAACCACACGGAGTTACCCCAACACT
GACTTCGGAGAACCGTTCAC

Downstream 100 bases:

>100_bases
GGGGAACGGACGATGAGCCAGACCGTAATGATCGTCGATGACGCGCTGTTCATGCGAAAAATCATACGCGGCATTCTGGA
GGAAAACGGATACCAGGTCG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MKYQAVIGLEVHVQLKTDSKIFCGCSTSFGSDPNSQTCPVCLGLPGALPVLNKKVVEFAIKAGLATNCSITQRSIFARKN
YFYPDLPKGYQISQFEDPVCQWGWLDIELNGETKRIGITRIHMEEDAGKLVHADVPELEDGSGVDLNRACTPLLEVVSEP
DLRSSDEAVSYLKQLHQIVTWLGICDGNMEEGSFRCDANVSVMEQGAEKFGTRAEIKNINSFRFVKQAIEYEIQRQIDLI
EDGGTVVQETRLFDPDNGVTRSMRSKEEAHDYRYFPDPDLVPLVIDDDWIQRVRQGLPELPEHKKQRFMSEFSIPAYDAE
VLTASRPLAEYYEECVACHHDGKAVSNWVMGEVTRGLNDSGLSIENCPVTPRLLGGLLKLMDNGTISGKIAKTVFDEMWK
SGKEPAVIVEEQGLVQVSDNGAILAIIDEIMAKNQGQVAEYRSGKDKLFGFFVGQVMKASKGKANPSLVNELLLEKLKG

Sequences:

>Translated_479_residues
MKYQAVIGLEVHVQLKTDSKIFCGCSTSFGSDPNSQTCPVCLGLPGALPVLNKKVVEFAIKAGLATNCSITQRSIFARKN
YFYPDLPKGYQISQFEDPVCQWGWLDIELNGETKRIGITRIHMEEDAGKLVHADVPELEDGSGVDLNRACTPLLEVVSEP
DLRSSDEAVSYLKQLHQIVTWLGICDGNMEEGSFRCDANVSVMEQGAEKFGTRAEIKNINSFRFVKQAIEYEIQRQIDLI
EDGGTVVQETRLFDPDNGVTRSMRSKEEAHDYRYFPDPDLVPLVIDDDWIQRVRQGLPELPEHKKQRFMSEFSIPAYDAE
VLTASRPLAEYYEECVACHHDGKAVSNWVMGEVTRGLNDSGLSIENCPVTPRLLGGLLKLMDNGTISGKIAKTVFDEMWK
SGKEPAVIVEEQGLVQVSDNGAILAIIDEIMAKNQGQVAEYRSGKDKLFGFFVGQVMKASKGKANPSLVNELLLEKLKG
>Mature_479_residues
MKYQAVIGLEVHVQLKTDSKIFCGCSTSFGSDPNSQTCPVCLGLPGALPVLNKKVVEFAIKAGLATNCSITQRSIFARKN
YFYPDLPKGYQISQFEDPVCQWGWLDIELNGETKRIGITRIHMEEDAGKLVHADVPELEDGSGVDLNRACTPLLEVVSEP
DLRSSDEAVSYLKQLHQIVTWLGICDGNMEEGSFRCDANVSVMEQGAEKFGTRAEIKNINSFRFVKQAIEYEIQRQIDLI
EDGGTVVQETRLFDPDNGVTRSMRSKEEAHDYRYFPDPDLVPLVIDDDWIQRVRQGLPELPEHKKQRFMSEFSIPAYDAE
VLTASRPLAEYYEECVACHHDGKAVSNWVMGEVTRGLNDSGLSIENCPVTPRLLGGLLKLMDNGTISGKIAKTVFDEMWK
SGKEPAVIVEEQGLVQVSDNGAILAIIDEIMAKNQGQVAEYRSGKDKLFGFFVGQVMKASKGKANPSLVNELLLEKLKG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=498, Percent_Identity=38.1526104417671, Blast_Score=331, Evalue=8e-91,
Organism=Caenorhabditis elegans, GI115534094, Length=303, Percent_Identity=38.9438943894389, Blast_Score=213, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6319391, Length=509, Percent_Identity=28.4872298624754, Blast_Score=205, Evalue=1e-53,
Organism=Drosophila melanogaster, GI45550808, Length=495, Percent_Identity=34.5454545454545, Blast_Score=285, Evalue=5e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_PELPD (A1ATL4)

Other databases:

- EMBL:   CP000482
- RefSeq:   YP_902742.1
- ProteinModelPortal:   A1ATL4
- SMR:   A1ATL4
- STRING:   A1ATL4
- GeneID:   4573962
- GenomeReviews:   CP000482_GR
- KEGG:   ppd:Ppro_3089
- eggNOG:   COG0064
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- PhylomeDB:   A1ATL4
- ProtClustDB:   PRK05477
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 53249; Mature: 53249

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYQAVIGLEVHVQLKTDSKIFCGCSTSFGSDPNSQTCPVCLGLPGALPVLNKKVVEFAI
CCCEEEEEEEEEEEEECCCEEEEEECCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHH
KAGLATNCSITQRSIFARKNYFYPDLPKGYQISQFEDPVCQWGWLDIELNGETKRIGITR
HHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEEE
IHMEEDAGKLVHADVPELEDGSGVDLNRACTPLLEVVSEPDLRSSDEAVSYLKQLHQIVT
EEECCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
WLGICDGNMEEGSFRCDANVSVMEQGAEKFGTRAEIKNINSFRFVKQAIEYEIQRQIDLI
HHHHCCCCCCCCCEEECCCHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHEE
EDGGTVVQETRLFDPDNGVTRSMRSKEEAHDYRYFPDPDLVPLVIDDDWIQRVRQGLPEL
CCCCCEEECCEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHCCCCC
PEHKKQRFMSEFSIPAYDAEVLTASRPLAEYYEECVACHHDGKAVSNWVMGEVTRGLNDS
HHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCC
GLSIENCPVTPRLLGGLLKLMDNGTISGKIAKTVFDEMWKSGKEPAVIVEEQGLVQVSDN
CCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCEEEECCC
GAILAIIDEIMAKNQGQVAEYRSGKDKLFGFFVGQVMKASKGKANPSLVNELLLEKLKG
CCEEHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKYQAVIGLEVHVQLKTDSKIFCGCSTSFGSDPNSQTCPVCLGLPGALPVLNKKVVEFAI
CCCEEEEEEEEEEEEECCCEEEEEECCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHH
KAGLATNCSITQRSIFARKNYFYPDLPKGYQISQFEDPVCQWGWLDIELNGETKRIGITR
HHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEEE
IHMEEDAGKLVHADVPELEDGSGVDLNRACTPLLEVVSEPDLRSSDEAVSYLKQLHQIVT
EEECCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
WLGICDGNMEEGSFRCDANVSVMEQGAEKFGTRAEIKNINSFRFVKQAIEYEIQRQIDLI
HHHHCCCCCCCCCEEECCCHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHEE
EDGGTVVQETRLFDPDNGVTRSMRSKEEAHDYRYFPDPDLVPLVIDDDWIQRVRQGLPEL
CCCCCEEECCEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHCCCCC
PEHKKQRFMSEFSIPAYDAEVLTASRPLAEYYEECVACHHDGKAVSNWVMGEVTRGLNDS
HHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCC
GLSIENCPVTPRLLGGLLKLMDNGTISGKIAKTVFDEMWKSGKEPAVIVEEQGLVQVSDN
CCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCEEEECCC
GAILAIIDEIMAKNQGQVAEYRSGKDKLFGFFVGQVMKASKGKANPSLVNELLLEKLKG
CCEEHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA