Click here to switch to the map view.

The map label for this gene is ligA

Identifier: 118581474

GI number: 118581474

Start: 3361139

End: 3363253

Strand: Direct

Name: ligA

Synonym: Ppro_3071

Alternate gene names: 118581474

Gene position: 3361139-3363253 (Clockwise)

Preceding gene: 118581470

Following gene: 118581475

Centisome position: 83.86

GC content: 42.88

Gene sequence:

>2115_bases
ATGCAAAACTCTCCAACCACGGAACAAACCAAAAACATTGTTGCAGAACGTATTCAAGAGCTTCGTCAACTCATAGCACA
CAACAATCGTTTATATTATGAAAACATTCCTCCACGGCGAGAGATACATGATTATGAATATGACGAGCTTTTCAAGGAAT
TGCAGGAACTTGAATCAAGATTTCCGGAGTTTCAATCAGCGGACAGTCCGACTCAAATAGTAGGTGGTTCTGTTTCGGAA
GGATTTAAAAAAGTCCAGCACTCAGTCCCGATGTTGTCTATTGAAAACAAGCCAGTTACAAAGATGCTTTCTGAAGTAAG
GAGTATTATCAAAGAATTAAAAGATGATGCCATATCGATTGATATTGTAGCCGAGCCAAAAATTGACGGTCTTTCATGCT
CAATACGTTACGAAAAGCATCAATTGGTACGGGCAGCCACTAGAGGAGACGGTCTTGAAGGCGAAGACATTACTGTTAAT
GTCCACTCAATTAGTGAGATACCTAAAATATTGCCGCTTGATGCCCCAGAAGTAATAGAAATACGGGGCGAAGTGTACAT
GTCGAATTCTGATTTTAAACAGTACACCGCGCAGCAAAACAAGATTGGGGAAAAGCCTCCTGAAAACCCTCGTAATGCAG
CCGCCGGAAGCTTACGTCAGCTTGATCCTTCTGTAACCGCATCTCGTCCGCTCCGCTTCTTTGCGTATGCGTGGGGAGAG
ATTTCCAATTCCTTTGCTAAGAGCCAGTGGGACGCCTTACAGACCTTGCGTGGATGGGGATTTAAGGTATGTGATGATAT
CCGGCTATTGTATTCTTCAGACGAGCTAAATTCATATTTTGAGGAAATGCAAGAGCGCCGTAGTGAACTCGATTTCACCA
TTGATGGGATAGTATACAAGCTTAACAGCCTATCGCTTCAGGAACGAGTAGGACAGACCAACCGGGCTCCACGATGGGCT
GCAGCCCAAAAATTCCCTCCGGAAAAGCGAGAGACTCTTCTACAAAATATCACAATATCTGTTGGTCGAAGCGGAGCACT
CACCCCAGTCGCAGAACTTTTGCCGGTCCGCCTGCTTGGTACTACAGTTTCAAATGCAACCCTGCATAACCAGGATGAAG
TAGAGTGTAAAGATTTCCGAATCGATGACACCATAGTAGTACAGCGTGCTGGGGACGTTATTCCTCAAGTGGTTTCTGTC
GTCATAGAAAAAAGGCTTTCTGGTAGCATTCCGTTTGTTTTTCCGTCAGCCTGCCCAGTCTGTGGTAGCAAAGCTGTTCG
AGAACCAAGAGAAGCTGTCTGGAAATGTACTGGAGGTCTGACATGTCCCGCACAATCATTGGAAAGACTAAAACATTTCG
TATCTCGTGATGCCTTTAACATTGATGGACTTGGCGAAAAAAACATCGAGTTGTTTTACAATAAAGGTCTGTTAGCATCT
CCAGTAGATATATTCAGACTTGAAGAAATTCTTTCACCACCATTACTTTGGCAACAAAAACCATCAGAATTTAAACCACT
GCAAGAATGGGATGGATGGGGTGAGCTTTCCGCCAACAATCTTTTTAGGGCTATTCGGACCAAACAAAAGATCACACTTT
ATCGTTTTATATATGCGCTTGGTATTCCAAAAGTTGGCGAGGTTACAGCAAAGATACTTGCTGATAACTATGTCAGTCTC
GACAATTGGCAAAGCTCAATGCTTAAAGCAGCAGAAAGAGAGTCTGAGTGCTATCAACATCTAATTTCAATTGACGGCAT
CGGCTCAGTCGTTGCAGATGAAATAGTAAGTTTTTTTGCAGAAGCCCATAACATCCAAGTCCTCGATTCTCTGAAAAATT
ATCTTAGCGTTGAGGATTTTACCAAACCGGCCATAATATCCTCCAATATTTCCGGAAAAATCGTGGTTTTTACAGGAGAA
TTGGAGAAGCGCAGCCGCAAGGCAGCAAAAATTGAAGCAGAAAAATTTGGGGCAAAGGTAGCTACCGATGTATCAAGAAA
AACTGACATTGTTATTGCAGGGACAGACCCAGGATCAAAATTGCGCAAGGCACAAGAACTAGGAATTAAAATTCTATCTG
AAGATGAGTGGGAGCATCTGATCAATGAAAAATGA

Upstream 100 bases:

>100_bases
AGTTCTCTTCCATGGGGATTTCCCGCTAGTCGGTGCGATTGAAATCAGGAGCATAATACTGTACGCTGGGGCGTTGTCAA
CCACATCCCGAGGTTACCCG

Downstream 100 bases:

>100_bases
TCAAATTGAGACTTTGGCAACAAAAAGGTCTTCAGACTTAAACGCCAATTTTATAGATGGATTTGCTGATGGCTGGTTTA
TGGCATTAAAAGAAAGCTAC

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 704; Mature: 704

Protein sequence:

>704_residues
MQNSPTTEQTKNIVAERIQELRQLIAHNNRLYYENIPPRREIHDYEYDELFKELQELESRFPEFQSADSPTQIVGGSVSE
GFKKVQHSVPMLSIENKPVTKMLSEVRSIIKELKDDAISIDIVAEPKIDGLSCSIRYEKHQLVRAATRGDGLEGEDITVN
VHSISEIPKILPLDAPEVIEIRGEVYMSNSDFKQYTAQQNKIGEKPPENPRNAAAGSLRQLDPSVTASRPLRFFAYAWGE
ISNSFAKSQWDALQTLRGWGFKVCDDIRLLYSSDELNSYFEEMQERRSELDFTIDGIVYKLNSLSLQERVGQTNRAPRWA
AAQKFPPEKRETLLQNITISVGRSGALTPVAELLPVRLLGTTVSNATLHNQDEVECKDFRIDDTIVVQRAGDVIPQVVSV
VIEKRLSGSIPFVFPSACPVCGSKAVREPREAVWKCTGGLTCPAQSLERLKHFVSRDAFNIDGLGEKNIELFYNKGLLAS
PVDIFRLEEILSPPLLWQQKPSEFKPLQEWDGWGELSANNLFRAIRTKQKITLYRFIYALGIPKVGEVTAKILADNYVSL
DNWQSSMLKAAERESECYQHLISIDGIGSVVADEIVSFFAEAHNIQVLDSLKNYLSVEDFTKPAIISSNISGKIVVFTGE
LEKRSRKAAKIEAEKFGAKVATDVSRKTDIVIAGTDPGSKLRKAQELGIKILSEDEWEHLINEK

Sequences:

>Translated_704_residues
MQNSPTTEQTKNIVAERIQELRQLIAHNNRLYYENIPPRREIHDYEYDELFKELQELESRFPEFQSADSPTQIVGGSVSE
GFKKVQHSVPMLSIENKPVTKMLSEVRSIIKELKDDAISIDIVAEPKIDGLSCSIRYEKHQLVRAATRGDGLEGEDITVN
VHSISEIPKILPLDAPEVIEIRGEVYMSNSDFKQYTAQQNKIGEKPPENPRNAAAGSLRQLDPSVTASRPLRFFAYAWGE
ISNSFAKSQWDALQTLRGWGFKVCDDIRLLYSSDELNSYFEEMQERRSELDFTIDGIVYKLNSLSLQERVGQTNRAPRWA
AAQKFPPEKRETLLQNITISVGRSGALTPVAELLPVRLLGTTVSNATLHNQDEVECKDFRIDDTIVVQRAGDVIPQVVSV
VIEKRLSGSIPFVFPSACPVCGSKAVREPREAVWKCTGGLTCPAQSLERLKHFVSRDAFNIDGLGEKNIELFYNKGLLAS
PVDIFRLEEILSPPLLWQQKPSEFKPLQEWDGWGELSANNLFRAIRTKQKITLYRFIYALGIPKVGEVTAKILADNYVSL
DNWQSSMLKAAERESECYQHLISIDGIGSVVADEIVSFFAEAHNIQVLDSLKNYLSVEDFTKPAIISSNISGKIVVFTGE
LEKRSRKAAKIEAEKFGAKVATDVSRKTDIVIAGTDPGSKLRKAQELGIKILSEDEWEHLINEK
>Mature_704_residues
MQNSPTTEQTKNIVAERIQELRQLIAHNNRLYYENIPPRREIHDYEYDELFKELQELESRFPEFQSADSPTQIVGGSVSE
GFKKVQHSVPMLSIENKPVTKMLSEVRSIIKELKDDAISIDIVAEPKIDGLSCSIRYEKHQLVRAATRGDGLEGEDITVN
VHSISEIPKILPLDAPEVIEIRGEVYMSNSDFKQYTAQQNKIGEKPPENPRNAAAGSLRQLDPSVTASRPLRFFAYAWGE
ISNSFAKSQWDALQTLRGWGFKVCDDIRLLYSSDELNSYFEEMQERRSELDFTIDGIVYKLNSLSLQERVGQTNRAPRWA
AAQKFPPEKRETLLQNITISVGRSGALTPVAELLPVRLLGTTVSNATLHNQDEVECKDFRIDDTIVVQRAGDVIPQVVSV
VIEKRLSGSIPFVFPSACPVCGSKAVREPREAVWKCTGGLTCPAQSLERLKHFVSRDAFNIDGLGEKNIELFYNKGLLAS
PVDIFRLEEILSPPLLWQQKPSEFKPLQEWDGWGELSANNLFRAIRTKQKITLYRFIYALGIPKVGEVTAKILADNYVSL
DNWQSSMLKAAERESECYQHLISIDGIGSVVADEIVSFFAEAHNIQVLDSLKNYLSVEDFTKPAIISSNISGKIVVFTGE
LEKRSRKAAKIEAEKFGAKVATDVSRKTDIVIAGTDPGSKLRKAQELGIKILSEDEWEHLINEK

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=699, Percent_Identity=42.0600858369099, Blast_Score=507, Evalue=1e-144,
Organism=Escherichia coli, GI87082305, Length=542, Percent_Identity=24.7232472324723, Blast_Score=97, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_PELPD (A1ATJ6)

Other databases:

- EMBL:   CP000482
- RefSeq:   YP_902724.1
- ProteinModelPortal:   A1ATJ6
- STRING:   A1ATJ6
- GeneID:   4573976
- GenomeReviews:   CP000482_GR
- KEGG:   ppd:Ppro_3071
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- PhylomeDB:   A1ATJ6
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 79187; Mature: 79187

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 127-127 BINDING 125-125 BINDING 148-148 BINDING 184-184 BINDING 300-300 BINDING 324-324

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNSPTTEQTKNIVAERIQELRQLIAHNNRLYYENIPPRREIHDYEYDELFKELQELESR
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
FPEFQSADSPTQIVGGSVSEGFKKVQHSVPMLSIENKPVTKMLSEVRSIIKELKDDAISI
CCCCCCCCCCHHHCCCCHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHCCCCEEE
DIVAEPKIDGLSCSIRYEKHQLVRAATRGDGLEGEDITVNVHSISEIPKILPLDAPEVIE
EEEECCCCCCCEEEEEEHHHHHHHHHHCCCCCCCCEEEEEECCHHHCCHHCCCCCCCEEE
IRGEVYMSNSDFKQYTAQQNKIGEKPPENPRNAAAGSLRQLDPSVTASRPLRFFAYAWGE
ECCEEEECCCHHHHHHHHHHHCCCCCCCCCCCHHHCCHHHCCCCCCCCCCHHHHHHHHHH
ISNSFAKSQWDALQTLRGWGFKVCDDIRLLYSSDELNSYFEEMQERRSELDFTIDGIVYK
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHEECCHHHHHHHHHHHHHHHHCCEEEHHHHHH
LNSLSLQERVGQTNRAPRWAAAQKFPPEKRETLLQNITISVGRSGALTPVAELLPVRLLG
HHHHHHHHHHCCCCCCCCCHHHCCCCCHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHH
TTVSNATLHNQDEVECKDFRIDDTIVVQRAGDVIPQVVSVVIEKRLSGSIPFVFPSACPV
CCCCCCEECCCCCCCHHHEECCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCC
CGSKAVREPREAVWKCTGGLTCPAQSLERLKHFVSRDAFNIDGLGEKNIELFYNKGLLAS
CCCHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCC
PVDIFRLEEILSPPLLWQQKPSEFKPLQEWDGWGELSANNLFRAIRTKQKITLYRFIYAL
CHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
GIPKVGEVTAKILADNYVSLDNWQSSMLKAAERESECYQHLISIDGIGSVVADEIVSFFA
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
EAHNIQVLDSLKNYLSVEDFTKPAIISSNISGKIVVFTGELEKRSRKAAKIEAEKFGAKV
HHCCHHHHHHHHHHHCHHHCCCCCEEECCCCCEEEEEECCHHHHHHHHHHHHHHHHCCHH
ATDVSRKTDIVIAGTDPGSKLRKAQELGIKILSEDEWEHLINEK
HHCCCCCCCEEEEECCCCHHHHHHHHHCCEEECCCHHHHHCCCC
>Mature Secondary Structure
MQNSPTTEQTKNIVAERIQELRQLIAHNNRLYYENIPPRREIHDYEYDELFKELQELESR
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
FPEFQSADSPTQIVGGSVSEGFKKVQHSVPMLSIENKPVTKMLSEVRSIIKELKDDAISI
CCCCCCCCCCHHHCCCCHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHCCCCEEE
DIVAEPKIDGLSCSIRYEKHQLVRAATRGDGLEGEDITVNVHSISEIPKILPLDAPEVIE
EEEECCCCCCCEEEEEEHHHHHHHHHHCCCCCCCCEEEEEECCHHHCCHHCCCCCCCEEE
IRGEVYMSNSDFKQYTAQQNKIGEKPPENPRNAAAGSLRQLDPSVTASRPLRFFAYAWGE
ECCEEEECCCHHHHHHHHHHHCCCCCCCCCCCHHHCCHHHCCCCCCCCCCHHHHHHHHHH
ISNSFAKSQWDALQTLRGWGFKVCDDIRLLYSSDELNSYFEEMQERRSELDFTIDGIVYK
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHEECCHHHHHHHHHHHHHHHHCCEEEHHHHHH
LNSLSLQERVGQTNRAPRWAAAQKFPPEKRETLLQNITISVGRSGALTPVAELLPVRLLG
HHHHHHHHHHCCCCCCCCCHHHCCCCCHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHH
TTVSNATLHNQDEVECKDFRIDDTIVVQRAGDVIPQVVSVVIEKRLSGSIPFVFPSACPV
CCCCCCEECCCCCCCHHHEECCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCC
CGSKAVREPREAVWKCTGGLTCPAQSLERLKHFVSRDAFNIDGLGEKNIELFYNKGLLAS
CCCHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCC
PVDIFRLEEILSPPLLWQQKPSEFKPLQEWDGWGELSANNLFRAIRTKQKITLYRFIYAL
CHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
GIPKVGEVTAKILADNYVSLDNWQSSMLKAAERESECYQHLISIDGIGSVVADEIVSFFA
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
EAHNIQVLDSLKNYLSVEDFTKPAIISSNISGKIVVFTGELEKRSRKAAKIEAEKFGAKV
HHCCHHHHHHHHHHHCHHHCCCCCEEECCCCCEEEEEECCHHHHHHHHHHHHHHHHCCHH
ATDVSRKTDIVIAGTDPGSKLRKAQELGIKILSEDEWEHLINEK
HHCCCCCCCEEEEECCCCHHHHHHHHHCCEEECCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA