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The map label for this gene is nrfA [H]

Identifier: 118581404

GI number: 118581404

Start: 3270891

End: 3272495

Strand: Reverse

Name: nrfA [H]

Synonym: Ppro_2999

Alternate gene names: 118581404

Gene position: 3272495-3270891 (Counterclockwise)

Preceding gene: 118581408

Following gene: 118581400

Centisome position: 81.65

GC content: 61.93

Gene sequence:

>1605_bases
ATGCGGAAGAATACTCTGGCGTGTGCCGCGGCCGTTATCGGGACGCTGGCGCTGTTGTCGCTCCCGGTCCTGACCACCGC
CGCCAAAGCTAAACCGGCCAATGACGGACGGGCGCAGTGCTACGAATGCCATGAAGAGGTCAAGGCTCTCAAGGAGGGGT
CACGCCATGCCTCCCTTTCCTGTGCGACCTGCCATGGCAAAATGGATGAACACATGAACGATCCCGAGAGCAGCAGGCCG
GTGACGGTCATCGATCAGGCCCTCTGCGCCAGGTGCCACAAACAGCAGTACGGCAGCTTTGTCACGGTGAATTACGAGGC
CCATGCCCGCAGGGAAAAGGGCATCCCCACCGGCCGCTCCCCCATGCAGGACAAGCTCCTGGCCGGCCACGGTTTCACCT
TCGAACATGCCGAACCCCGCGGCCATGCCTTCATGGTCACGGACCAGTTCATCGTGGACCGCTTCCAGGGGGGGCGCTTC
CAGTACAGGGATGGCTGGAAGGGGGTGGACAGGACCGGCAAGACCTGGGACGTGCTGACCGACACCGGCAGGAAGCTGCC
CGAGACCGCCATGGCGGGTAACCCCACCTGCATCCAGTGCAAGAGCTCCGACCATATTCTCAGGTGGAAATTCCTGGGGG
ACAGGGATCCCAAGGCGAAATGGGACCGCGGATCGGATGTCGTGGCGATTGCCAAGGATACCAACAACCCCATGGGCTGC
ATCCACTGCCACGACCCCCACGGCACTCAGCCGCGCGTGGTGCGTGACGCACTGATCCAGGCCATTGAAAAGGAGCCTGC
AACGAACATCTTCGCCAGAAACGGCAGGACCGATCTGAGGATGATCTCCTTCAGGGATGGTTTCCGCAAGATCGGCGTCA
TGGAGAAGGTCGACTCGCGCATGATGTGCGCCCAGTGCCACGTTGAGTACAACTGCAATGCCGGCAACCAGTGGAGCGAC
GGCGCGAAGGTGGGATTCGACGACCGCCGCACCAACCACTTCCCCTTGCGGAACTCGCTGCAGCTCCTCAAACACTACAG
GGAGCTCGACTTCTACGACTTCAAGCACGCCATAACCGGCGCCCGTCTGGTCAAGTTCCAGCACCCCGAGGCCGAGACCT
ATGCCGGCAGCGTGCACGACAGGGCCGGCGTTCAGTGCCACCAGTGCCACATGCCACAGCAGAAAGGGAAAAACGGCAAG
TCCTTCTCCAACCATGGCGTGATCCGTCCCAAAAACCACGTCAGAGAGTCCTGCCTGGGATGTCATCCCGACAGTACCGT
TGAGCGCAAGCTCTACCAGATCGAGGCGGTGCGGAATTACATCAGCGGCAAGATGCGCAAGTCGGAGTACTGGCTGGGGC
AGCTGATCGACAGCTATGTCGCTGCCCAGCGCATGGGGGTTACGGAGAGCGTGCTGGCCCAGGCGCGGGAGAAGCACGAG
GAGGCCCATGTATTGTGGGAGTACTGGACCGCCGAGAACTCGGACGGCTTCCACAATCCCGACCTTGCCCGCGAAAGCCT
GGCCGGCTCCATAGCCGCCTCCAAGGCCGGGGTCAGGATCCTGAACGACGCCATGCAGGTGGCCAGGAAGGGTGATGGGA
AGTAG

Upstream 100 bases:

>100_bases
CAGGTCAACCTAAAAATTTTCACCACGCCCGCTTTTTATCGCAGCTGGTATACTTCCTGTCATGACAAGGCAACCAATTC
ACGGGGAAAGGAGAAGTCTG

Downstream 100 bases:

>100_bases
CCGGCCAAGCCGCTGCTGAAAATCCACAGGCCCCGTTCCGAAAGGTTCGGGGCCTGTGGGTTTCCGGCCGCGGCACGCGC
CCGCCATCTCAAGGAAGTAT

Product: cytochrome c family protein

Products: NA

Alternate protein names: Ammonia-forming cytochrome c nitrite reductase; Cytochrome c nitrite reductase [H]

Number of amino acids: Translated: 534; Mature: 534

Protein sequence:

>534_residues
MRKNTLACAAAVIGTLALLSLPVLTTAAKAKPANDGRAQCYECHEEVKALKEGSRHASLSCATCHGKMDEHMNDPESSRP
VTVIDQALCARCHKQQYGSFVTVNYEAHARREKGIPTGRSPMQDKLLAGHGFTFEHAEPRGHAFMVTDQFIVDRFQGGRF
QYRDGWKGVDRTGKTWDVLTDTGRKLPETAMAGNPTCIQCKSSDHILRWKFLGDRDPKAKWDRGSDVVAIAKDTNNPMGC
IHCHDPHGTQPRVVRDALIQAIEKEPATNIFARNGRTDLRMISFRDGFRKIGVMEKVDSRMMCAQCHVEYNCNAGNQWSD
GAKVGFDDRRTNHFPLRNSLQLLKHYRELDFYDFKHAITGARLVKFQHPEAETYAGSVHDRAGVQCHQCHMPQQKGKNGK
SFSNHGVIRPKNHVRESCLGCHPDSTVERKLYQIEAVRNYISGKMRKSEYWLGQLIDSYVAAQRMGVTESVLAQAREKHE
EAHVLWEYWTAENSDGFHNPDLARESLAGSIAASKAGVRILNDAMQVARKGDGK

Sequences:

>Translated_534_residues
MRKNTLACAAAVIGTLALLSLPVLTTAAKAKPANDGRAQCYECHEEVKALKEGSRHASLSCATCHGKMDEHMNDPESSRP
VTVIDQALCARCHKQQYGSFVTVNYEAHARREKGIPTGRSPMQDKLLAGHGFTFEHAEPRGHAFMVTDQFIVDRFQGGRF
QYRDGWKGVDRTGKTWDVLTDTGRKLPETAMAGNPTCIQCKSSDHILRWKFLGDRDPKAKWDRGSDVVAIAKDTNNPMGC
IHCHDPHGTQPRVVRDALIQAIEKEPATNIFARNGRTDLRMISFRDGFRKIGVMEKVDSRMMCAQCHVEYNCNAGNQWSD
GAKVGFDDRRTNHFPLRNSLQLLKHYRELDFYDFKHAITGARLVKFQHPEAETYAGSVHDRAGVQCHQCHMPQQKGKNGK
SFSNHGVIRPKNHVRESCLGCHPDSTVERKLYQIEAVRNYISGKMRKSEYWLGQLIDSYVAAQRMGVTESVLAQAREKHE
EAHVLWEYWTAENSDGFHNPDLARESLAGSIAASKAGVRILNDAMQVARKGDGK
>Mature_534_residues
MRKNTLACAAAVIGTLALLSLPVLTTAAKAKPANDGRAQCYECHEEVKALKEGSRHASLSCATCHGKMDEHMNDPESSRP
VTVIDQALCARCHKQQYGSFVTVNYEAHARREKGIPTGRSPMQDKLLAGHGFTFEHAEPRGHAFMVTDQFIVDRFQGGRF
QYRDGWKGVDRTGKTWDVLTDTGRKLPETAMAGNPTCIQCKSSDHILRWKFLGDRDPKAKWDRGSDVVAIAKDTNNPMGC
IHCHDPHGTQPRVVRDALIQAIEKEPATNIFARNGRTDLRMISFRDGFRKIGVMEKVDSRMMCAQCHVEYNCNAGNQWSD
GAKVGFDDRRTNHFPLRNSLQLLKHYRELDFYDFKHAITGARLVKFQHPEAETYAGSVHDRAGVQCHQCHMPQQKGKNGK
SFSNHGVIRPKNHVRESCLGCHPDSTVERKLYQIEAVRNYISGKMRKSEYWLGQLIDSYVAAQRMGVTESVLAQAREKHE
EAHVLWEYWTAENSDGFHNPDLARESLAGSIAASKAGVRILNDAMQVARKGDGK

Specific function: Plays a role in nitrite reduction [H]

COG id: COG3303

COG function: function code P; Formate-dependent nitrite reductase, periplasmic cytochrome c552 subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome c-552 family [H]

Homologues:

Organism=Escherichia coli, GI1790506, Length=461, Percent_Identity=24.7288503253796, Blast_Score=102, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003321
- InterPro:   IPR017570
- InterPro:   IPR011031 [H]

Pfam domain/function: PF02335 Cytochrom_C552 [H]

EC number: =1.7.2.2 [H]

Molecular weight: Translated: 59911; Mature: 59911

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKNTLACAAAVIGTLALLSLPVLTTAAKAKPANDGRAQCYECHEEVKALKEGSRHASLS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEE
CATCHGKMDEHMNDPESSRPVTVIDQALCARCHKQQYGSFVTVNYEAHARREKGIPTGRS
EEECCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHCCCCCCCC
PMQDKLLAGHGFTFEHAEPRGHAFMVTDQFIVDRFQGGRFQYRDGWKGVDRTGKTWDVLT
CHHHHHHHCCCCEECCCCCCCCEEEEEHHHHHHHHCCCEEEECCCCCCCCCCCCEEEHEH
DTGRKLPETAMAGNPTCIQCKSSDHILRWKFLGDRDPKAKWDRGSDVVAIAKDTNNPMGC
HCCCCCCCHHHCCCCCEEEECCCCCEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCEEE
IHCHDPHGTQPRVVRDALIQAIEKEPATNIFARNGRTDLRMISFRDGFRKIGVMEKVDSR
EEECCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCEEEEEEHHHHHHHHHHHHHHHH
MMCAQCHVEYNCNAGNQWSDGAKVGFDDRRTNHFPLRNSLQLLKHYRELDFYDFKHAITG
HEEHHHCEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCC
ARLVKFQHPEAETYAGSVHDRAGVQCHQCHMPQQKGKNGKSFSNHGVIRPKNHVRESCLG
HHEEEEECCCCHHHCCCCCCCCCCEEHHCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHC
CHPDSTVERKLYQIEAVRNYISGKMRKSEYWLGQLIDSYVAAQRMGVTESVLAQAREKHE
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
EAHVLWEYWTAENSDGFHNPDLARESLAGSIAASKAGVRILNDAMQVARKGDGK
HHEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRKNTLACAAAVIGTLALLSLPVLTTAAKAKPANDGRAQCYECHEEVKALKEGSRHASLS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEE
CATCHGKMDEHMNDPESSRPVTVIDQALCARCHKQQYGSFVTVNYEAHARREKGIPTGRS
EEECCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHCCCCCCCC
PMQDKLLAGHGFTFEHAEPRGHAFMVTDQFIVDRFQGGRFQYRDGWKGVDRTGKTWDVLT
CHHHHHHHCCCCEECCCCCCCCEEEEEHHHHHHHHCCCEEEECCCCCCCCCCCCEEEHEH
DTGRKLPETAMAGNPTCIQCKSSDHILRWKFLGDRDPKAKWDRGSDVVAIAKDTNNPMGC
HCCCCCCCHHHCCCCCEEEECCCCCEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCEEE
IHCHDPHGTQPRVVRDALIQAIEKEPATNIFARNGRTDLRMISFRDGFRKIGVMEKVDSR
EEECCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCEEEEEEHHHHHHHHHHHHHHHH
MMCAQCHVEYNCNAGNQWSDGAKVGFDDRRTNHFPLRNSLQLLKHYRELDFYDFKHAITG
HEEHHHCEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCC
ARLVKFQHPEAETYAGSVHDRAGVQCHQCHMPQQKGKNGKSFSNHGVIRPKNHVRESCLG
HHEEEEECCCCHHHCCCCCCCCCCEEHHCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHC
CHPDSTVERKLYQIEAVRNYISGKMRKSEYWLGQLIDSYVAAQRMGVTESVLAQAREKHE
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
EAHVLWEYWTAENSDGFHNPDLARESLAGSIAASKAGVRILNDAMQVARKGDGK
HHEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]