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The map label for this gene is pilJ [H]

Identifier: 118581403

GI number: 118581403

Start: 3269123

End: 3270772

Strand: Direct

Name: pilJ [H]

Synonym: Ppro_2998

Alternate gene names: 118581403

Gene position: 3269123-3270772 (Clockwise)

Preceding gene: 118581402

Following gene: 118581405

Centisome position: 81.56

GC content: 63.45

Gene sequence:

>1650_bases
ATGTCAACCGCACGACTCTTCACCAGAATGAAGCTGCTCCACAAGCTGTTCCTGGCCATCACCGTCCTGAATATCCTCAC
CATCACCGCCTTCACCTGCTACAACTACGCGAACCAGAAAAAAACGGTACTACGGGGCATCGACGGCAGACTGCTGGCCA
GCGCCCAGGGGCTGAAACTGGCCATGGACCGTTTTCATGAACGGCTGGGCCGGGGGGAAACCATCCCCCCTTCCGAATTC
AGGAGGGAACTGGACGACCTGTCGGCCATGGTGAACCAGAGCGGGATCAACTACGCCTATACGGTGGTGATGAAGGATGG
AGGGGTCGCCTTCACCCTCTCCAGCTATACGAAGGAGGAGCTGGAAAAGGGGGAGCTGACCACCCTGTTTGAACCGTACA
GTGACGCTTCAACGGGGCTCAAGAAGGCACTGGCTGAGAACAAAACCGTTTATGAACAGTACACCGACAAGTGGGGGACC
TTCCGTTCGGTGTTTCTCCCATCACGCCTGGCCGACGGAGCATCCTACGCCATCGGCATCGACATCAGCATCGACGAGAT
CAATGCGACCCTGCGCAGGACCCTCCTGGGCTGCCTGTTCATCGGCCTGGGCGTGTTCATGGCCGGCACAGCCGTCGCCT
TCTGGGTGGCCTGGTACATCTCCCGGGGGATCGAGCGGCTGGCCAGCCACCTGCACCAGATCGCCGACGGCGATCTGGGA
GTCCTGATCAGCAAGACATCGGACGATGAGTTGGGCATGCTGGCCGATGACATGAACCGCATGGTGGAACGTCTCAGGCT
GCTTCTGGGTAGCGTCCGCCATGCCTCCGACAACGTCGTCGCCGCGGCCCACCAGGTTCACGCAAACTCCGCCAGCATGT
CCAACGGCGTGGACGCGGTGGCGGCCCAGGCAGTGGCGGTGGCCACGGCGGCGGAGGAGATGGCCGCCACCTCCCAGGAG
ATCGCCGGCAACTGCGACAGCACCGCCGCAAGCGTCAGGGAGACAGAGGGAACAGCCCGAAGCGCGGAGGGGGTTGTCCG
CCAGACGGTCCAGATGATGGACAGCATCGCGGAACTGGTGATGAAATCAGCCGCAACGGTGAAGGAGTTGGGGGCCAGCT
CCGACCAGATCGGCACGATCATCGGCGCCATCGACGAGATCGCCGACCAGACCAACCTGCTGGCCTTGAACGCGGCCATC
GAGGCGGCCAGGGCCGGTGAGCAGGGGCGCGGCTTTGCCGTTGTCGCCGACGAGGTTCGCGCCCTGGCGGAGAGGACCAG
CAGGGCGACCAGAGAGATCGCCGGCATGATCGGCTCCATCCAGGGCAACATCCAGGATGCGGCCCGCTCCATGGAAGAGG
GGGTCGCGCAGGTGGGCAGGGGAACCGAAGAGGCCGCCCGGTCGGGTGAGGCGCTGCAGGAAATCCTCCGGCACGCCAGC
GCCATCACCTCCCAAATCAGCCAGGTGGCCATTGCCGCCGAGGAGCAGACCACAACCACCGGCGAGATCAGCGGCAACAT
CAACCAGATCACCACCATCGCCCAGAAGACCGTCCAGGAGGTCCAGAATTCCGTTGCCGCGGCCCGTCAGCTGATGGACC
TGGCCCACGAACTGCAGGAGCAGGTCGGGCAGTTCAGGCTCGCCCCATAA

Upstream 100 bases:

>100_bases
AGCGATGTCTTCTGTGGCTGCGATTGACGGAGAAGACAGGCCAACGACACACATGCGCAACAATCGATACATCAACCTGG
GTACAGAAAGGAAACTATCG

Downstream 100 bases:

>100_bases
GTCGACCAGGACAGTACAATACTTCCTTGAGATGGCGGGCGCGTGCCGCGGCCGGAAACCCACAGGCCCCGAACCTTTCG
GAACGGGGCCTGTGGATTTT

Product: methyl-accepting chemotaxis sensory transducer

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 549; Mature: 548

Protein sequence:

>549_residues
MSTARLFTRMKLLHKLFLAITVLNILTITAFTCYNYANQKKTVLRGIDGRLLASAQGLKLAMDRFHERLGRGETIPPSEF
RRELDDLSAMVNQSGINYAYTVVMKDGGVAFTLSSYTKEELEKGELTTLFEPYSDASTGLKKALAENKTVYEQYTDKWGT
FRSVFLPSRLADGASYAIGIDISIDEINATLRRTLLGCLFIGLGVFMAGTAVAFWVAWYISRGIERLASHLHQIADGDLG
VLISKTSDDELGMLADDMNRMVERLRLLLGSVRHASDNVVAAAHQVHANSASMSNGVDAVAAQAVAVATAAEEMAATSQE
IAGNCDSTAASVRETEGTARSAEGVVRQTVQMMDSIAELVMKSAATVKELGASSDQIGTIIGAIDEIADQTNLLALNAAI
EAARAGEQGRGFAVVADEVRALAERTSRATREIAGMIGSIQGNIQDAARSMEEGVAQVGRGTEEAARSGEALQEILRHAS
AITSQISQVAIAAEEQTTTTGEISGNINQITTIAQKTVQEVQNSVAAARQLMDLAHELQEQVGQFRLAP

Sequences:

>Translated_549_residues
MSTARLFTRMKLLHKLFLAITVLNILTITAFTCYNYANQKKTVLRGIDGRLLASAQGLKLAMDRFHERLGRGETIPPSEF
RRELDDLSAMVNQSGINYAYTVVMKDGGVAFTLSSYTKEELEKGELTTLFEPYSDASTGLKKALAENKTVYEQYTDKWGT
FRSVFLPSRLADGASYAIGIDISIDEINATLRRTLLGCLFIGLGVFMAGTAVAFWVAWYISRGIERLASHLHQIADGDLG
VLISKTSDDELGMLADDMNRMVERLRLLLGSVRHASDNVVAAAHQVHANSASMSNGVDAVAAQAVAVATAAEEMAATSQE
IAGNCDSTAASVRETEGTARSAEGVVRQTVQMMDSIAELVMKSAATVKELGASSDQIGTIIGAIDEIADQTNLLALNAAI
EAARAGEQGRGFAVVADEVRALAERTSRATREIAGMIGSIQGNIQDAARSMEEGVAQVGRGTEEAARSGEALQEILRHAS
AITSQISQVAIAAEEQTTTTGEISGNINQITTIAQKTVQEVQNSVAAARQLMDLAHELQEQVGQFRLAP
>Mature_548_residues
STARLFTRMKLLHKLFLAITVLNILTITAFTCYNYANQKKTVLRGIDGRLLASAQGLKLAMDRFHERLGRGETIPPSEFR
RELDDLSAMVNQSGINYAYTVVMKDGGVAFTLSSYTKEELEKGELTTLFEPYSDASTGLKKALAENKTVYEQYTDKWGTF
RSVFLPSRLADGASYAIGIDISIDEINATLRRTLLGCLFIGLGVFMAGTAVAFWVAWYISRGIERLASHLHQIADGDLGV
LISKTSDDELGMLADDMNRMVERLRLLLGSVRHASDNVVAAAHQVHANSASMSNGVDAVAAQAVAVATAAEEMAATSQEI
AGNCDSTAASVRETEGTARSAEGVVRQTVQMMDSIAELVMKSAATVKELGASSDQIGTIIGAIDEIADQTNLLALNAAIE
AARAGEQGRGFAVVADEVRALAERTSRATREIAGMIGSIQGNIQDAARSMEEGVAQVGRGTEEAARSGEALQEILRHASA
ITSQISQVAIAAEEQTTTTGEISGNINQITTIAQKTVQEVQNSVAAARQLMDLAHELQEQVGQFRLAP

Specific function: May be a part of a signal-transduction system that regulates twitching motility by controlling pilus function (extension and retraction) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI1787690, Length=365, Percent_Identity=31.2328767123288, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI2367378, Length=565, Percent_Identity=26.9026548672566, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI1788195, Length=339, Percent_Identity=31.858407079646, Blast_Score=132, Evalue=7e-32,
Organism=Escherichia coli, GI1788194, Length=334, Percent_Identity=30.5389221556886, Blast_Score=125, Evalue=7e-30,
Organism=Escherichia coli, GI1789453, Length=361, Percent_Identity=26.0387811634349, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004090
- InterPro:   IPR004089
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00015 MCPsignal [H]

EC number: NA

Molecular weight: Translated: 58841; Mature: 58710

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS50885 HAMP ; PS50111 CHEMOTAXIS_TRANSDUC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTARLFTRMKLLHKLFLAITVLNILTITAFTCYNYANQKKTVLRGIDGRLLASAQGLKL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCHHHHHHCHHHH
AMDRFHERLGRGETIPPSEFRRELDDLSAMVNQSGINYAYTVVMKDGGVAFTLSSYTKEE
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCEEEEEHHHHHHH
LEKGELTTLFEPYSDASTGLKKALAENKTVYEQYTDKWGTFRSVFLPSRLADGASYAIGI
HHCCCCCEEECCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
DISIDEINATLRRTLLGCLFIGLGVFMAGTAVAFWVAWYISRGIERLASHLHQIADGDLG
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VLISKTSDDELGMLADDMNRMVERLRLLLGSVRHASDNVVAAAHQVHANSASMSNGVDAV
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCHHHH
AAQAVAVATAAEEMAATSQEIAGNCDSTAASVRETEGTARSAEGVVRQTVQMMDSIAELV
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
MKSAATVKELGASSDQIGTIIGAIDEIADQTNLLALNAAIEAARAGEQGRGFAVVADEVR
HHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCEEEHHHHHH
ALAERTSRATREIAGMIGSIQGNIQDAARSMEEGVAQVGRGTEEAARSGEALQEILRHAS
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHCHHHHHHHHHHHH
AITSQISQVAIAAEEQTTTTGEISGNINQITTIAQKTVQEVQNSVAAARQLMDLAHELQE
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVGQFRLAP
HHHCCCCCC
>Mature Secondary Structure 
STARLFTRMKLLHKLFLAITVLNILTITAFTCYNYANQKKTVLRGIDGRLLASAQGLKL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCHHHHHHCHHHH
AMDRFHERLGRGETIPPSEFRRELDDLSAMVNQSGINYAYTVVMKDGGVAFTLSSYTKEE
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCEEEEEHHHHHHH
LEKGELTTLFEPYSDASTGLKKALAENKTVYEQYTDKWGTFRSVFLPSRLADGASYAIGI
HHCCCCCEEECCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
DISIDEINATLRRTLLGCLFIGLGVFMAGTAVAFWVAWYISRGIERLASHLHQIADGDLG
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VLISKTSDDELGMLADDMNRMVERLRLLLGSVRHASDNVVAAAHQVHANSASMSNGVDAV
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCHHHH
AAQAVAVATAAEEMAATSQEIAGNCDSTAASVRETEGTARSAEGVVRQTVQMMDSIAELV
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
MKSAATVKELGASSDQIGTIIGAIDEIADQTNLLALNAAIEAARAGEQGRGFAVVADEVR
HHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCEEEHHHHHH
ALAERTSRATREIAGMIGSIQGNIQDAARSMEEGVAQVGRGTEEAARSGEALQEILRHAS
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHCHHHHHHHHHHHH
AITSQISQVAIAAEEQTTTTGEISGNINQITTIAQKTVQEVQNSVAAARQLMDLAHELQE
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVGQFRLAP
HHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7908398; 10984043 [H]