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The map label for this gene is sppA [H]

Identifier: 118581390

GI number: 118581390

Start: 3254846

End: 3255808

Strand: Direct

Name: sppA [H]

Synonym: Ppro_2985

Alternate gene names: 118581390

Gene position: 3254846-3255808 (Clockwise)

Preceding gene: 118581389

Following gene: 118581393

Centisome position: 81.21

GC content: 63.76

Gene sequence:

>963_bases
ATGAAACGTCCCCTTGCCCTGTTCCTTGCGTCGCTCGCCCTGCTGGTCCTGCACGGCTGCGCCTTCGTGACCGTTCCCCT
AGCCCCCCGGCCCAGCCCGCTTAAGGAGCAGGTCCTGGAGGGTGAGGGCGCCAAAAAGATCCTGCTGCTGGATATCAGCG
GAACGATCTCGGAAAAGGAAAAGCCGGGCGGCCTGGTGGGAGCGGACAGACCGTCGACGGTCTCGCTGATCCGCGAAGCC
CTGCAGAAGGCGGAGAAGGACGACAGGATCGCCGGCGTCATCCTGCGCATCGACTCCCCCGGCGGAAGCGTGACCGCCAG
CGACATCATCCATCACGATATTACGGAATTCAAGAAGCGGCGCAGGATACCGGTGCATGCCTGCATCATGGGGGTGGGGG
CTTCGGGGGGCTACTACGCGGCTGCCGCGGCCGACCGGATCATTGCCCACCCCACCGCCATCACCGGCAGCATCGGCGTG
ATCCTGATGAAATTCAACCTGGCAGGCCTGCTGGGCAAGTTGGGAATTGAGGAGCAGGCCGTAAAATCGGGGGACAAGAA
GGACTTTTTCTCCCTGTTCCGCCGGGCAACGCCCGAGGAGGAGAAGCTGGCCCAGGAGATCATCAACCAGCTGCACAGCC
GCTTCCTGGACGTGGTCATGCAGCGCCCCGGCAACAGGCTGTCGCGGGATGAACTGGCCAGGCTGGCCGACGGGCGCATC
TACACCGCCGGTCAGGCGCTTCAGGCGCGGCTGGTGGACGGAACCGGCTATCTTGACGATGTCATCTCCGCGATGAGGAA
ACAGATCAAGGATGAACAAGCGCGGGTGGTCAGCTACTACCGCCCCGGCAGCTACCTGGGGAGCATCTATGACGGCAGGG
AGGCGAAGAGCAGCCTGGTCGGCATGCTGGGCGCGACGAGCGAGTTTTCAGGCGGCAGCTTCATGTACCTGTGGCAGCCG
TGA

Upstream 100 bases:

>100_bases
GAACTACCTGGGGCAGCGCCGCTGACACCCCCTGCCTCGTCACGGCTCCAGGTTGCTGCCCATCTCCCGGGAAACCCTGT
TCCCATGAAACGAGGTTGCC

Downstream 100 bases:

>100_bases
GATCCCGCAGCGGAGCTCGACAGGTCAGCTCTGCTTCGAGACGGCTCGACAGGGGGAGGGAAGCAGCCCCGCGATCCGCG
CGAAATTCTCCACCAGCCGA

Product: signal peptide peptidase SppA, 36K type

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MKRPLALFLASLALLVLHGCAFVTVPLAPRPSPLKEQVLEGEGAKKILLLDISGTISEKEKPGGLVGADRPSTVSLIREA
LQKAEKDDRIAGVILRIDSPGGSVTASDIIHHDITEFKKRRRIPVHACIMGVGASGGYYAAAAADRIIAHPTAITGSIGV
ILMKFNLAGLLGKLGIEEQAVKSGDKKDFFSLFRRATPEEEKLAQEIINQLHSRFLDVVMQRPGNRLSRDELARLADGRI
YTAGQALQARLVDGTGYLDDVISAMRKQIKDEQARVVSYYRPGSYLGSIYDGREAKSSLVGMLGATSEFSGGSFMYLWQP

Sequences:

>Translated_320_residues
MKRPLALFLASLALLVLHGCAFVTVPLAPRPSPLKEQVLEGEGAKKILLLDISGTISEKEKPGGLVGADRPSTVSLIREA
LQKAEKDDRIAGVILRIDSPGGSVTASDIIHHDITEFKKRRRIPVHACIMGVGASGGYYAAAAADRIIAHPTAITGSIGV
ILMKFNLAGLLGKLGIEEQAVKSGDKKDFFSLFRRATPEEEKLAQEIINQLHSRFLDVVMQRPGNRLSRDELARLADGRI
YTAGQALQARLVDGTGYLDDVISAMRKQIKDEQARVVSYYRPGSYLGSIYDGREAKSSLVGMLGATSEFSGGSFMYLWQP
>Mature_320_residues
MKRPLALFLASLALLVLHGCAFVTVPLAPRPSPLKEQVLEGEGAKKILLLDISGTISEKEKPGGLVGADRPSTVSLIREA
LQKAEKDDRIAGVILRIDSPGGSVTASDIIHHDITEFKKRRRIPVHACIMGVGASGGYYAAAAADRIIAHPTAITGSIGV
ILMKFNLAGLLGKLGIEEQAVKSGDKKDFFSLFRRATPEEEKLAQEIINQLHSRFLDVVMQRPGNRLSRDELARLADGRI
YTAGQALQARLVDGTGYLDDVISAMRKQIKDEQARVVSYYRPGSYLGSIYDGREAKSSLVGMLGATSEFSGGSFMYLWQP

Specific function: Digestion of cleaved signal peptides. Required for efficient processing of precursors under conditions of hyper-secretion [H]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=240, Percent_Identity=28.3333333333333, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787527, Length=174, Percent_Identity=31.0344827586207, Blast_Score=78, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 34674; Mature: 34674

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRPLALFLASLALLVLHGCAFVTVPLAPRPSPLKEQVLEGEGAKKILLLDISGTISEKE
CCCHHHHHHHHHHHHHHHCHHHEEEECCCCCCHHHHHHHCCCCCCEEEEEEECCCCCCCC
KPGGLVGADRPSTVSLIREALQKAEKDDRIAGVILRIDSPGGSVTASDIIHHDITEFKKR
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCEEHHHHHHHHHHHHHHH
RRIPVHACIMGVGASGGYYAAAAADRIIAHPTAITGSIGVILMKFNLAGLLGKLGIEEQA
CCCCHHHHHEECCCCCCEEHHHHHHHHHCCCCHHCCCHHHEEEHHHHHHHHHHCCCHHHH
VKSGDKKDFFSLFRRATPEEEKLAQEIINQLHSRFLDVVMQRPGNRLSRDELARLADGRI
HHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCE
YTAGQALQARLVDGTGYLDDVISAMRKQIKDEQARVVSYYRPGSYLGSIYDGREAKSSLV
EECCHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHCCHHHHHHHH
GMLGATSEFSGGSFMYLWQP
HHHCCCCCCCCCCEEEEECC
>Mature Secondary Structure
MKRPLALFLASLALLVLHGCAFVTVPLAPRPSPLKEQVLEGEGAKKILLLDISGTISEKE
CCCHHHHHHHHHHHHHHHCHHHEEEECCCCCCHHHHHHHCCCCCCEEEEEEECCCCCCCC
KPGGLVGADRPSTVSLIREALQKAEKDDRIAGVILRIDSPGGSVTASDIIHHDITEFKKR
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCEEHHHHHHHHHHHHHHH
RRIPVHACIMGVGASGGYYAAAAADRIIAHPTAITGSIGVILMKFNLAGLLGKLGIEEQA
CCCCHHHHHEECCCCCCEEHHHHHHHHHCCCCHHCCCHHHEEEHHHHHHHHHHCCCHHHH
VKSGDKKDFFSLFRRATPEEEKLAQEIINQLHSRFLDVVMQRPGNRLSRDELARLADGRI
HHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCE
YTAGQALQARLVDGTGYLDDVISAMRKQIKDEQARVVSYYRPGSYLGSIYDGREAKSSLV
EECCHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHCCHHHHHHHH
GMLGATSEFSGGSFMYLWQP
HHHCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 10455123 [H]