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The map label for this gene is hyfB [H]

Identifier: 118581194

GI number: 118581194

Start: 3048428

End: 3050329

Strand: Reverse

Name: hyfB [H]

Synonym: Ppro_2787

Alternate gene names: 118581194

Gene position: 3050329-3048428 (Counterclockwise)

Preceding gene: 118581195

Following gene: 118581193

Centisome position: 76.11

GC content: 61.51

Gene sequence:

>1902_bases
ATGACTCCTGAACAAGCAGTACTCTTATCGATCGGCATCTGCGCCGCCGGTGCCGTAGTGACGCTTTTGCTCTCCATGAG
CAAGAGCCTGTCCGGCTGGCTTTCCTTCCTGACCATGGTTGCCAGCGGTGTCTTGATCGTGCCGCGGGCGGTGCAGGTAC
TGACCAGCGGTCCTGCCAACCCGCACCATCCCGAGGCATTTTACACCCTGGGTGGTCACACGCTGCGCCTGTACGTTGAC
GGCCTGAGCTCCATCTTTTTGCTTCTTGCGGTGCTGATCGGGGTCTGTGCGGCCCTGTACTCCATCGTCCATGTGGAGAA
ATTCCCCACCAAGAGCGCGGCGATGTATCATCCGTTCCTGCTTCTGTTCGTGGGCGGCATGTACGGCATCCTCTCCATCA
CCGACACCATGTGGTTCTTCTGCGCCTTCTGGCAGCTGATGACCATCCCCAGCTTCATCCTGATGCTGTTCGAGAGTGAG
AAGCCCGGCATCAAGGGCGCTGCCTTCAAGTACCTTGTCTTCATGGAAGCCGCCATGGTAGCCGCCATGGCCGGCGCCGC
CATGATTGCCGGCGGCCCGGCCGAGCCTGGCCTTGCCTACGACTTCGACGTTCTTTCCGAAGGCATCCCGGCTCTGGTCG
CCACCAACCCGGGTGCGCTCACTGGCTGCCTGGCCCTGTTCCTTGTTGGCTTCGGCATTAAAGTCGGCATGTGGCCCTTC
GGTCAGCTGTGGGTACCCGACGCCTATGCCGCTGCTCCGTCCCCGGTAACCGCTCTCATCTCCGGCGTCATGAGCAAGAC
CGGCGTCTACGGCCTGATGAGGACCTTCCTGTGGCTGGTACCGGCCGACGTGCTTGGCCAGTACGACATGAGCAGGTGGG
GCATGGCCCTGGCCATCCTGGGCACCATCACCCTGTTCACCGGCACCATGCAGGGTATGAAACAGGACGACACCAAACGG
CTCCTGGCCTTCAGCTCCGTCGGTCAGCTGGGCTATATCATCCTTGCCCTTGGCGCCTGCGTCGCCCTGGTCCCCTCCGG
TGACAACAGACTGCTTGCTCTGGCTGCCATCGCCTTCTGCGGCGGCCTGCTTCACGCCATCAACCACGGCATCTTCAAGG
GTCTTCTGTTCTTCAGCGCCGGCTCCCTGTACCAGGCAACCGGCACCCAGGACTTAAACAAAATGGGCGGCCTGTGGAAG
TACATGCCGGTCACCGGCTTTGCCGTCCTGATCGCCTCCTTCGGCATCGCCGGTGTACCGCTGTTAAACGGCTTTGTCAG
CAAATGGAGCATCTACGTCGCCACCATCCAGGGGAGCACGAGCGCCAAATACCTTGCCGTCTGCGCCATCATCGCCATCC
TCACCAGCGCCATGACGCTTGCCATGTACATCAAATTCTTCGGCGTCGCCTTTTTGTCCCGCCAGAGCACGGTGGTCAAA
CAGCAGGCCGCCAAGCAGGGGAATCTTGAAGTGGGCATGACCCAGCAGCTTCCGCAGCTGATCATGGCGCTTCTGTGCAT
CGTCATCGGCCTTGCCCCCGGCGCCGTGTACCAGGTTCTGGGCCAGGTGCTTAACACCAGCCGCCAGGGCTTTGCCGAGA
AACTGGCCGACGCCAACACCATGTCCGCCGGCGCCACCACGGGTCTCTTCGCCATCGGCGGCGGCGCCCTGTACGTGCCG
ATCGTTATCGCTATCGTCCTGGCAGTAGCCTTCGTCATCGTGTACACCTTCTCCAAGCAGGGCGGTTCCACCCGCAAGAG
CGGCGACGCCTGGCTGTGCGGGTATGCCCGCGAAGAAGAGTGCTATCGTTATGCCGCCTCCAACTTCTACGGTGAAATCA
AGCGCTGCTTCGGGTGGCTCGGCGGCAATCCGCGTCCCCAGACACGCATCAAGGAAGACTAA

Upstream 100 bases:

>100_bases
CCCGGTATTTACCTACGAACAGCTGAAAACGCTCGGGGAAGATATCTAACGTTTCCCGACTACGAGTGCATGGCTTTGGG
CCATTCGCAGAGGTGCAATA

Downstream 100 bases:

>100_bases
CCATGGTAAGTAAAAAGCTCCAGCAACTGAAAGACAAATTCGGCTCCGCCATCCAGCAGGCCGACGTAGTCAACGACATG
CGTCTGTTGGTCTACATCGA

Product: NADH dehydrogenase (quinone)

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 633; Mature: 632

Protein sequence:

>633_residues
MTPEQAVLLSIGICAAGAVVTLLLSMSKSLSGWLSFLTMVASGVLIVPRAVQVLTSGPANPHHPEAFYTLGGHTLRLYVD
GLSSIFLLLAVLIGVCAALYSIVHVEKFPTKSAAMYHPFLLLFVGGMYGILSITDTMWFFCAFWQLMTIPSFILMLFESE
KPGIKGAAFKYLVFMEAAMVAAMAGAAMIAGGPAEPGLAYDFDVLSEGIPALVATNPGALTGCLALFLVGFGIKVGMWPF
GQLWVPDAYAAAPSPVTALISGVMSKTGVYGLMRTFLWLVPADVLGQYDMSRWGMALAILGTITLFTGTMQGMKQDDTKR
LLAFSSVGQLGYIILALGACVALVPSGDNRLLALAAIAFCGGLLHAINHGIFKGLLFFSAGSLYQATGTQDLNKMGGLWK
YMPVTGFAVLIASFGIAGVPLLNGFVSKWSIYVATIQGSTSAKYLAVCAIIAILTSAMTLAMYIKFFGVAFLSRQSTVVK
QQAAKQGNLEVGMTQQLPQLIMALLCIVIGLAPGAVYQVLGQVLNTSRQGFAEKLADANTMSAGATTGLFAIGGGALYVP
IVIAIVLAVAFVIVYTFSKQGGSTRKSGDAWLCGYAREEECYRYAASNFYGEIKRCFGWLGGNPRPQTRIKED

Sequences:

>Translated_633_residues
MTPEQAVLLSIGICAAGAVVTLLLSMSKSLSGWLSFLTMVASGVLIVPRAVQVLTSGPANPHHPEAFYTLGGHTLRLYVD
GLSSIFLLLAVLIGVCAALYSIVHVEKFPTKSAAMYHPFLLLFVGGMYGILSITDTMWFFCAFWQLMTIPSFILMLFESE
KPGIKGAAFKYLVFMEAAMVAAMAGAAMIAGGPAEPGLAYDFDVLSEGIPALVATNPGALTGCLALFLVGFGIKVGMWPF
GQLWVPDAYAAAPSPVTALISGVMSKTGVYGLMRTFLWLVPADVLGQYDMSRWGMALAILGTITLFTGTMQGMKQDDTKR
LLAFSSVGQLGYIILALGACVALVPSGDNRLLALAAIAFCGGLLHAINHGIFKGLLFFSAGSLYQATGTQDLNKMGGLWK
YMPVTGFAVLIASFGIAGVPLLNGFVSKWSIYVATIQGSTSAKYLAVCAIIAILTSAMTLAMYIKFFGVAFLSRQSTVVK
QQAAKQGNLEVGMTQQLPQLIMALLCIVIGLAPGAVYQVLGQVLNTSRQGFAEKLADANTMSAGATTGLFAIGGGALYVP
IVIAIVLAVAFVIVYTFSKQGGSTRKSGDAWLCGYAREEECYRYAASNFYGEIKRCFGWLGGNPRPQTRIKED
>Mature_632_residues
TPEQAVLLSIGICAAGAVVTLLLSMSKSLSGWLSFLTMVASGVLIVPRAVQVLTSGPANPHHPEAFYTLGGHTLRLYVDG
LSSIFLLLAVLIGVCAALYSIVHVEKFPTKSAAMYHPFLLLFVGGMYGILSITDTMWFFCAFWQLMTIPSFILMLFESEK
PGIKGAAFKYLVFMEAAMVAAMAGAAMIAGGPAEPGLAYDFDVLSEGIPALVATNPGALTGCLALFLVGFGIKVGMWPFG
QLWVPDAYAAAPSPVTALISGVMSKTGVYGLMRTFLWLVPADVLGQYDMSRWGMALAILGTITLFTGTMQGMKQDDTKRL
LAFSSVGQLGYIILALGACVALVPSGDNRLLALAAIAFCGGLLHAINHGIFKGLLFFSAGSLYQATGTQDLNKMGGLWKY
MPVTGFAVLIASFGIAGVPLLNGFVSKWSIYVATIQGSTSAKYLAVCAIIAILTSAMTLAMYIKFFGVAFLSRQSTVVKQ
QAAKQGNLEVGMTQQLPQLIMALLCIVIGLAPGAVYQVLGQVLNTSRQGFAEKLADANTMSAGATTGLFAIGGGALYVPI
VIAIVLAVAFVIVYTFSKQGGSTRKSGDAWLCGYAREEECYRYAASNFYGEIKRCFGWLGGNPRPQTRIKED

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=231, Percent_Identity=32.9004329004329, Blast_Score=91, Evalue=3e-18,
Organism=Escherichia coli, GI1788827, Length=538, Percent_Identity=29.5539033457249, Blast_Score=190, Evalue=3e-49,
Organism=Escherichia coli, GI2367154, Length=395, Percent_Identity=29.873417721519, Blast_Score=129, Evalue=8e-31,
Organism=Escherichia coli, GI1788613, Length=380, Percent_Identity=27.3684210526316, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1788831, Length=363, Percent_Identity=27.5482093663912, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI1788829, Length=447, Percent_Identity=27.5167785234899, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1788614, Length=440, Percent_Identity=27.5, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI145693160, Length=362, Percent_Identity=25.414364640884, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 67115; Mature: 66984

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPEQAVLLSIGICAAGAVVTLLLSMSKSLSGWLSFLTMVASGVLIVPRAVQVLTSGPAN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHCCCCC
PHHPEAFYTLGGHTLRLYVDGLSSIFLLLAVLIGVCAALYSIVHVEKFPTKSAAMYHPFL
CCCCCEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
LLFVGGMYGILSITDTMWFFCAFWQLMTIPSFILMLFESEKPGIKGAAFKYLVFMEAAMV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
AAMAGAAMIAGGPAEPGLAYDFDVLSEGIPALVATNPGALTGCLALFLVGFGIKVGMWPF
HHHHHHHHCCCCCCCCCCEEEHHHHHCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCC
GQLWVPDAYAAAPSPVTALISGVMSKTGVYGLMRTFLWLVPADVLGQYDMSRWGMALAIL
CCEECCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCHHHHHHHHHHH
GTITLFTGTMQGMKQDDTKRLLAFSSVGQLGYIILALGACVALVPSGDNRLLALAAIAFC
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHH
GGLLHAINHGIFKGLLFFSAGSLYQATGTQDLNKMGGLWKYMPVTGFAVLIASFGIAGVP
HHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHCCCEEHHHHHHHHHHHHHHCCCCHH
LLNGFVSKWSIYVATIQGSTSAKYLAVCAIIAILTSAMTLAMYIKFFGVAFLSRQSTVVK
HHHHHHHCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QQAAKQGNLEVGMTQQLPQLIMALLCIVIGLAPGAVYQVLGQVLNTSRQGFAEKLADANT
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
MSAGATTGLFAIGGGALYVPIVIAIVLAVAFVIVYTFSKQGGSTRKSGDAWLCGYAREEE
CCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCHHH
CYRYAASNFYGEIKRCFGWLGGNPRPQTRIKED
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TPEQAVLLSIGICAAGAVVTLLLSMSKSLSGWLSFLTMVASGVLIVPRAVQVLTSGPAN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHCCCCC
PHHPEAFYTLGGHTLRLYVDGLSSIFLLLAVLIGVCAALYSIVHVEKFPTKSAAMYHPFL
CCCCCEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
LLFVGGMYGILSITDTMWFFCAFWQLMTIPSFILMLFESEKPGIKGAAFKYLVFMEAAMV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
AAMAGAAMIAGGPAEPGLAYDFDVLSEGIPALVATNPGALTGCLALFLVGFGIKVGMWPF
HHHHHHHHCCCCCCCCCCEEEHHHHHCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCC
GQLWVPDAYAAAPSPVTALISGVMSKTGVYGLMRTFLWLVPADVLGQYDMSRWGMALAIL
CCEECCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCHHHHHHHHHHH
GTITLFTGTMQGMKQDDTKRLLAFSSVGQLGYIILALGACVALVPSGDNRLLALAAIAFC
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHH
GGLLHAINHGIFKGLLFFSAGSLYQATGTQDLNKMGGLWKYMPVTGFAVLIASFGIAGVP
HHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHCCCEEHHHHHHHHHHHHHHCCCCHH
LLNGFVSKWSIYVATIQGSTSAKYLAVCAIIAILTSAMTLAMYIKFFGVAFLSRQSTVVK
HHHHHHHCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QQAAKQGNLEVGMTQQLPQLIMALLCIVIGLAPGAVYQVLGQVLNTSRQGFAEKLADANT
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
MSAGATTGLFAIGGGALYVPIVIAIVLAVAFVIVYTFSKQGGSTRKSGDAWLCGYAREEE
CCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCHHH
CYRYAASNFYGEIKRCFGWLGGNPRPQTRIKED
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 2016588 [H]