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The map label for this gene is yogA [H]

Identifier: 118581144

GI number: 118581144

Start: 2995263

End: 2996249

Strand: Reverse

Name: yogA [H]

Synonym: Ppro_2736

Alternate gene names: 118581144

Gene position: 2996249-2995263 (Counterclockwise)

Preceding gene: 118581152

Following gene: 118581141

Centisome position: 74.76

GC content: 60.69

Gene sequence:

>987_bases
ATGAAAGCTGTTTTGATGGATGGTTTCGGTGGAATCGAGGTTCTCGGGCTGGGAGAGGTCGAGCGTCCCGTGCCGGGTGA
GGGACAGGTGCTGGTGAAGACGTTTGCCACCTCCATCAACCGCCCCGACATGGTGCAGCGTCAAGGCAAGTATCCGCCGC
CAGCGGGCGATTCCGATATCCTCGGCCTGGAGGTGGCCGGCGTCATCGAGGAGCTGGGAGCCGGCGTCATCGGTTGGAAG
AAGGGGGATCGGGTCATGTCGCTGGTGGGAGGCGGCGGCTATGCCGAGTATGCCGTGGCCTATGCCAGCCACCTGATGCC
CATCCCTGAGAGCATGAGCTTCGAAGAGGCTGCCTGCGTCTGCGAATCCTCCATCACCGCCTTTCTGAACGTATTCATCC
TGGGCGAGTTCAAGGATGGCCAGACCGCCATCCTGCATGGCGGCGGCGGCGGGGTCAACACTGCGGCGATCCAGCTCTGT
CGTGCCCTGACCCCTGGTTGCCGTCTGATCGTCACCTCTTCTCCCGAGAAGATGGAGCGGGTAAAACAGGTCGGTGCCGA
TTTCCTGATCAACTACCGCGAGACCCCCGATTTCAGCGATCTGGTCAAGGAGTATACCGGCAAGAAGGGGGTCGATCTGA
TCCTTGACCACATCGGCGCCAAGTACTTGGCACCCAACATGAACTCCCTGGCCTACAAGGGGCGCATGGTGGTTATCGGC
GTTACCAGCGGCATCAAGGCAGAGCTGAACCTGGCCCTGATGATGGTCAAGCGCCAGCAGATCATCGGCAGCGTGCTCCG
CTCTCGCCCGGTCTCGGAGAAGGGGGAAATCGTGGCTGAGTTCACCCGTCGGGCCCTGCCCAAATTCGCCGACCGTACCA
TTGTGCCGATCATCGAGAAGGTCTTCACCATCGATCAGGTGGTGGAGGCCCACCGCATGATGGAAGAGGACCGCCATTTC
GGCAAGATCGTGCTGAAGATACAGTAG

Upstream 100 bases:

>100_bases
TCCGGGTGAAGCGGCTACATTTTTGCGGATTCCTGGCGCTTTGCGGGAGTCGTCTTCTGTCGTCGGTTACACATATTCTT
GCTGTCTGGAGGTCATACCC

Downstream 100 bases:

>100_bases
GTTTCCTGCAATGCAATGGATCAAGAGCCCCTGGTGCTGGTCGCCAGGGGCTCTTTTTTCCGGAATCTGTGATTACGGGA
GGATGGGGCGGGTTGACCGA

Product: alcohol dehydrogenase

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MKAVLMDGFGGIEVLGLGEVERPVPGEGQVLVKTFATSINRPDMVQRQGKYPPPAGDSDILGLEVAGVIEELGAGVIGWK
KGDRVMSLVGGGGYAEYAVAYASHLMPIPESMSFEEAACVCESSITAFLNVFILGEFKDGQTAILHGGGGGVNTAAIQLC
RALTPGCRLIVTSSPEKMERVKQVGADFLINYRETPDFSDLVKEYTGKKGVDLILDHIGAKYLAPNMNSLAYKGRMVVIG
VTSGIKAELNLALMMVKRQQIIGSVLRSRPVSEKGEIVAEFTRRALPKFADRTIVPIIEKVFTIDQVVEAHRMMEEDRHF
GKIVLKIQ

Sequences:

>Translated_328_residues
MKAVLMDGFGGIEVLGLGEVERPVPGEGQVLVKTFATSINRPDMVQRQGKYPPPAGDSDILGLEVAGVIEELGAGVIGWK
KGDRVMSLVGGGGYAEYAVAYASHLMPIPESMSFEEAACVCESSITAFLNVFILGEFKDGQTAILHGGGGGVNTAAIQLC
RALTPGCRLIVTSSPEKMERVKQVGADFLINYRETPDFSDLVKEYTGKKGVDLILDHIGAKYLAPNMNSLAYKGRMVVIG
VTSGIKAELNLALMMVKRQQIIGSVLRSRPVSEKGEIVAEFTRRALPKFADRTIVPIIEKVFTIDQVVEAHRMMEEDRHF
GKIVLKIQ
>Mature_328_residues
MKAVLMDGFGGIEVLGLGEVERPVPGEGQVLVKTFATSINRPDMVQRQGKYPPPAGDSDILGLEVAGVIEELGAGVIGWK
KGDRVMSLVGGGGYAEYAVAYASHLMPIPESMSFEEAACVCESSITAFLNVFILGEFKDGQTAILHGGGGGVNTAAIQLC
RALTPGCRLIVTSSPEKMERVKQVGADFLINYRETPDFSDLVKEYTGKKGVDLILDHIGAKYLAPNMNSLAYKGRMVVIG
VTSGIKAELNLALMMVKRQQIIGSVLRSRPVSEKGEIVAEFTRRALPKFADRTIVPIIEKVFTIDQVVEAHRMMEEDRHF
GKIVLKIQ

Specific function: Unknown

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI22538446, Length=333, Percent_Identity=40.5405405405405, Blast_Score=241, Evalue=6e-64,
Organism=Homo sapiens, GI22538444, Length=333, Percent_Identity=40.5405405405405, Blast_Score=241, Evalue=6e-64,
Organism=Homo sapiens, GI194239674, Length=332, Percent_Identity=33.7349397590361, Blast_Score=144, Evalue=9e-35,
Organism=Homo sapiens, GI13236495, Length=332, Percent_Identity=33.7349397590361, Blast_Score=144, Evalue=9e-35,
Organism=Homo sapiens, GI18379349, Length=344, Percent_Identity=31.6860465116279, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI24308257, Length=348, Percent_Identity=25.2873563218391, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI194239676, Length=206, Percent_Identity=35.9223300970874, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI41872631, Length=311, Percent_Identity=28.9389067524116, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI28557745, Length=231, Percent_Identity=29.8701298701299, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI197927207, Length=179, Percent_Identity=31.8435754189944, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI47519420, Length=375, Percent_Identity=26.1333333333333, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1790485, Length=298, Percent_Identity=32.5503355704698, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI87082125, Length=367, Percent_Identity=27.5204359673025, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787863, Length=360, Percent_Identity=25.5555555555556, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI226510941, Length=331, Percent_Identity=22.9607250755287, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17507255, Length=330, Percent_Identity=27.5757575757576, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI71987554, Length=377, Percent_Identity=24.4031830238727, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17536829, Length=223, Percent_Identity=30.0448430493274, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI212642053, Length=314, Percent_Identity=26.4331210191083, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319520, Length=248, Percent_Identity=27.4193548387097, Blast_Score=83, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6323961, Length=336, Percent_Identity=22.9166666666667, Blast_Score=73, Evalue=7e-14,
Organism=Drosophila melanogaster, GI19920632, Length=305, Percent_Identity=27.5409836065574, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI221330659, Length=305, Percent_Identity=27.5409836065574, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24581345, Length=331, Percent_Identity=25.3776435045317, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI45550423, Length=168, Percent_Identity=32.7380952380952, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 35497; Mature: 35497

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAVLMDGFGGIEVLGLGEVERPVPGEGQVLVKTFATSINRPDMVQRQGKYPPPAGDSDI
CCEEEECCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHCCCCCCCCCCCCE
LGLEVAGVIEELGAGVIGWKKGDRVMSLVGGGGYAEYAVAYASHLMPIPESMSFEEAACV
EHHHHHHHHHHHCCCEEEECCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCHHHHHHH
CESSITAFLNVFILGEFKDGQTAILHGGGGGVNTAAIQLCRALTPGCRLIVTSSPEKMER
HHHHHHHHHHHHEEEEECCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCHHHHHH
VKQVGADFLINYRETPDFSDLVKEYTGKKGVDLILDHIGAKYLAPNMNSLAYKGRMVVIG
HHHCCHHHEEECCCCCCHHHHHHHHCCCCCHHHHHHHHCCHHCCCCCCHHEECCEEEEEE
VTSGIKAELNLALMMVKRQQIIGSVLRSRPVSEKGEIVAEFTRRALPKFADRTIVPIIEK
ECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHH
VFTIDQVVEAHRMMEEDRHFGKIVLKIQ
HHHHHHHHHHHHHHHHCCCCCEEEEEEC
>Mature Secondary Structure
MKAVLMDGFGGIEVLGLGEVERPVPGEGQVLVKTFATSINRPDMVQRQGKYPPPAGDSDI
CCEEEECCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHCCCCCCCCCCCCE
LGLEVAGVIEELGAGVIGWKKGDRVMSLVGGGGYAEYAVAYASHLMPIPESMSFEEAACV
EHHHHHHHHHHHCCCEEEECCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCHHHHHHH
CESSITAFLNVFILGEFKDGQTAILHGGGGGVNTAAIQLCRALTPGCRLIVTSSPEKMER
HHHHHHHHHHHHEEEEECCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCHHHHHH
VKQVGADFLINYRETPDFSDLVKEYTGKKGVDLILDHIGAKYLAPNMNSLAYKGRMVVIG
HHHCCHHHEEECCCCCCHHHHHHHHCCCCCHHHHHHHHCCHHCCCCCCHHEECCEEEEEE
VTSGIKAELNLALMMVKRQQIIGSVLRSRPVSEKGEIVAEFTRRALPKFADRTIVPIIEK
ECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHH
VFTIDQVVEAHRMMEEDRHFGKIVLKIQ
HHHHHHHHHHHHHHHHCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.0024 {1,2-naphthoquinone}} 0.26 {1,4-naphthoquinone}} 0.065 {1,4-benzoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]