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The map label for this gene is rotA [H]

Identifier: 118581138

GI number: 118581138

Start: 2986962

End: 2987531

Strand: Direct

Name: rotA [H]

Synonym: Ppro_2730

Alternate gene names: 118581138

Gene position: 2986962-2987531 (Clockwise)

Preceding gene: 118581125

Following gene: 118581139

Centisome position: 74.52

GC content: 59.82

Gene sequence:

>570_bases
ATGCTGAGAAGATGTGTCGTTGTGCTTTTTGCACTGCTGTTGTCGGCCAGCGTTGCCCTGGCGGAGGGAAAGGACAATCC
GCTGCTGGTGATGGAAACCAGCCTGGGGAGCGTGAAGCTGGAACTGTTCCGCGACAAGGCGCCGCTGAGCGTCGCGAACT
TTCTGGCCTACGTGGCCACCGGTTTTTACGACGGAACCATATTCCACCGGGTGATCCGGGGCTTCATGATCCAGGGGGGA
GGATTCGATACGGAATTCAGAGAGAAGCCCACCCGGGCGCCCATCAGGAACGAGGCGGACAACGGACTCGAGAACAGGCG
CGGAACCCTGGCCATGGCCCGCACTGCGGATCCGGGCAGCGCCACGTCCCAGTTCTTCATCAACCAGGTGGACAACCGGA
CTCTGAACCGCCCCTCTCCCGATGGTCATGGGTATGCCGTATTCGGCAGGGTTGTTGAAGGGATGGAGGTGATCGACAGG
ATCGCCAGGGTAAGGACCGGGTACCGCGGGGGATTTCAGGATGTGCCGCTGGAACGGGTGGAAATCAGGTCAATCAGGGT
GATCAAATAA

Upstream 100 bases:

>100_bases
CCCCCTGTTCCCAATGGTTGATCTGGACCGAGTCCTGTCGTATAACCATAAGCCATGGGTAGTATCGCGGTTTCATCATC
CAAAAAAACGAGGAGTTGTC

Downstream 100 bases:

>100_bases
TCGGGCGACGGTGCGCCGTCACCTGAAACACAGAGATGCCGGCTACGGCTGTCCGGTCGGGGGAGGAAGGGATGCACGAG
AAAAACCGGAAGTCGTTTTT

Product: peptidylprolyl isomerase

Products: NA

Alternate protein names: PPIase; Rotamase [H]

Number of amino acids: Translated: 189; Mature: 189

Protein sequence:

>189_residues
MLRRCVVVLFALLLSASVALAEGKDNPLLVMETSLGSVKLELFRDKAPLSVANFLAYVATGFYDGTIFHRVIRGFMIQGG
GFDTEFREKPTRAPIRNEADNGLENRRGTLAMARTADPGSATSQFFINQVDNRTLNRPSPDGHGYAVFGRVVEGMEVIDR
IARVRTGYRGGFQDVPLERVEIRSIRVIK

Sequences:

>Translated_189_residues
MLRRCVVVLFALLLSASVALAEGKDNPLLVMETSLGSVKLELFRDKAPLSVANFLAYVATGFYDGTIFHRVIRGFMIQGG
GFDTEFREKPTRAPIRNEADNGLENRRGTLAMARTADPGSATSQFFINQVDNRTLNRPSPDGHGYAVFGRVVEGMEVIDR
IARVRTGYRGGFQDVPLERVEIRSIRVIK
>Mature_189_residues
MLRRCVVVLFALLLSASVALAEGKDNPLLVMETSLGSVKLELFRDKAPLSVANFLAYVATGFYDGTIFHRVIRGFMIQGG
GFDTEFREKPTRAPIRNEADNGLENRRGTLAMARTADPGSATSQFFINQVDNRTLNRPSPDGHGYAVFGRVVEGMEVIDR
IARVRTGYRGGFQDVPLERVEIRSIRVIK

Specific function: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. This protein is not essential for growth. Presumably plays a role in signal transduction [H]

COG id: COG0652

COG function: function code O; Peptidyl-prolyl cis-trans isomerase (rotamase) - cyclophilin family

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase cyclophilin-type domain [H]

Homologues:

Organism=Homo sapiens, GI19557636, Length=170, Percent_Identity=31.7647058823529, Blast_Score=84, Evalue=7e-17,
Organism=Homo sapiens, GI24308049, Length=174, Percent_Identity=34.4827586206897, Blast_Score=84, Evalue=9e-17,
Organism=Homo sapiens, GI7706339, Length=159, Percent_Identity=35.2201257861635, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI64276486, Length=148, Percent_Identity=31.7567567567568, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI22547215, Length=161, Percent_Identity=32.2981366459627, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI22547212, Length=161, Percent_Identity=32.2981366459627, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI7657473, Length=161, Percent_Identity=32.2981366459627, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI5454154, Length=158, Percent_Identity=32.9113924050633, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI1789763, Length=184, Percent_Identity=52.7173913043478, Blast_Score=187, Evalue=4e-49,
Organism=Escherichia coli, GI1786736, Length=162, Percent_Identity=56.1728395061728, Blast_Score=179, Evalue=7e-47,
Organism=Caenorhabditis elegans, GI71980594, Length=164, Percent_Identity=31.7073170731707, Blast_Score=84, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17539496, Length=169, Percent_Identity=34.9112426035503, Blast_Score=83, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI71980590, Length=140, Percent_Identity=32.8571428571429, Blast_Score=82, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17532641, Length=177, Percent_Identity=32.7683615819209, Blast_Score=77, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17506311, Length=177, Percent_Identity=31.0734463276836, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI19922376, Length=172, Percent_Identity=30.8139534883721, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17986117, Length=173, Percent_Identity=32.3699421965318, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24652460, Length=151, Percent_Identity=29.8013245033113, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19922912, Length=162, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015891
- InterPro:   IPR020892
- InterPro:   IPR002130 [H]

Pfam domain/function: PF00160 Pro_isomerase [H]

EC number: =5.2.1.8 [H]

Molecular weight: Translated: 20963; Mature: 20963

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: PS00170 CSA_PPIASE_1 ; PS50072 CSA_PPIASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRRCVVVLFALLLSASVALAEGKDNPLLVMETSLGSVKLELFRDKAPLSVANFLAYVAT
CHHHHHHHHHHHHHHHHHHEECCCCCCEEEEECCCCCEEEEEHHCCCCHHHHHHHHHHHH
GFYDGTIFHRVIRGFMIQGGGFDTEFREKPTRAPIRNEADNGLENRRGTLAMARTADPGS
CCCCHHHHHHHHHHHEECCCCCCCHHHCCCCCCCCCCCCCCCHHCCCCCEEEEECCCCCC
ATSQFFINQVDNRTLNRPSPDGHGYAVFGRVVEGMEVIDRIARVRTGYRGGFQDVPLERV
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCE
EIRSIRVIK
ECCEEEECC
>Mature Secondary Structure
MLRRCVVVLFALLLSASVALAEGKDNPLLVMETSLGSVKLELFRDKAPLSVANFLAYVAT
CHHHHHHHHHHHHHHHHHHEECCCCCCEEEEECCCCCEEEEEHHCCCCHHHHHHHHHHHH
GFYDGTIFHRVIRGFMIQGGGFDTEFREKPTRAPIRNEADNGLENRRGTLAMARTADPGS
CCCCHHHHHHHHHHHEECCCCCCCHHHCCCCCCCCCCCCCCCHHCCCCCEEEEECCCCCC
ATSQFFINQVDNRTLNRPSPDGHGYAVFGRVVEGMEVIDRIARVRTGYRGGFQDVPLERV
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCE
EIRSIRVIK
ECCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7948017 [H]