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The map label for this gene is sbcD [H]

Identifier: 118580981

GI number: 118580981

Start: 2780961

End: 2782193

Strand: Direct

Name: sbcD [H]

Synonym: Ppro_2569

Alternate gene names: 118580981

Gene position: 2780961-2782193 (Clockwise)

Preceding gene: 118580980

Following gene: 118580982

Centisome position: 69.39

GC content: 66.91

Gene sequence:

>1233_bases
ATGAAAATCCTGCACACATCGGACTGGCACATCGGCCGCGCCCTGTACGGCCGCAAACGCTACGAAGAGTTCGAGCTCTT
TCTCGACTGGCTGACAACCTGCATCCAGAGCGAGCAGATCGAGGCGTTGCTGGTGGCGGGAGACGTGTTCGACAACGGCA
CCCCCAGCAACCGGGCGCTGGAGTTGTACTACCGCTTCCTCTGCCGCGCGGCCGGCGCTGGCTGCCGGCAGGTGGTGGTC
ACGGCCGGCAACCACGACTCCCCCTCGCTCCTCAATGCCCCCCGCGAACTGTTGCGCCACCTGGACGTGCATGTGGTCGG
CTGCATGACCGAGACGGTCGAGGACGAGCTGGTTGTGGTGAAACAGGAGGACGGCCAGCCCGGCCTGATCGTCTGCGCCG
TCCCCTACCTGCGCGACCGGGACATCCGCAGGGCCGAGGCGGGAGAGACCTTCGAGGACAAGGGGCGCAAGCTGGTGGAG
GGCATCGCCGACCACTATTGCCGGGTGGCCGACGCTGCCGCTGCCCTGAACGCCCGGCTGGGGGGCAACATCCCCATCGT
CGCCATGGGGCACCTCTTCACCAGCGGCGGCACAACCCTGGAGGGGGACGGCGTGCGCGAACTGTATGTGGGGAACCTGG
GTCAGGTGACCAGCGATCTTTTACCCGACAGTTTTCGCTACCTGGCCCTGGGCCACCTGCATGTGGCCCAGCGGGTGAAT
GGTTCGAGCACTCGGCGCTACTGCGGCTCCCCCCTGCCCATGAGCTTCGGCGAGGCAAATCAGCGCAAGCTGGTCCTGGC
TGTCGAACTGGACGCAGCGGGGCTCAGGGTGGATGAGCTCCCAGTCCCCTGTTTTCAGCAGCTGGCCACGCTGCGCGGGG
ACTGGGATCACATCAGCCGGCGGATCCAGGAGCTGAAGCGGGAGTCCGCCGCCATCTGGCTGGAGGTCGTCTACGAAGGG
GGCGAGATCGTGGGCGACCTGCAGGAGCGGCTGCGTGAGCTTGTGGAGGGGACATCCCTGGAGATCCTGCGCACCAGGAA
CATGCGCTTGGTGGATCAGGCCCTGAGCGCCATGGCAACCGAGGAGACCCTGGACGACCTGAGCGTGGACGACGTGTTCG
CCCGCTGCCTGGACGCCCATGGGGTGCCAGACGAACAGCGCCCCGACCTGGCCGCACTCTTCGCCGAGACCATGGCCGCG
CTGCACGAGGACGACAGCAGGGGGGCCCCATGA

Upstream 100 bases:

>100_bases
CCCTTCCGCTTGATCAGTTTTCGCAAAACGAGGTTCGCCCCCCACTCACGACGGCGTTAATAACAGCACCGTTCCACTCT
CTTCCCGGAAAGAACCGACC

Downstream 100 bases:

>100_bases
AAATTCTGCGCCTCAGCTTCCGCAACCTGAACTCCCTGGCCGGCGACTGGGATCTGGACCTGACCCATCCGGCCTACGTC
TCCAGCGGCATCTTCGCCAT

Product: nuclease SbcCD subunit D

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MKILHTSDWHIGRALYGRKRYEEFELFLDWLTTCIQSEQIEALLVAGDVFDNGTPSNRALELYYRFLCRAAGAGCRQVVV
TAGNHDSPSLLNAPRELLRHLDVHVVGCMTETVEDELVVVKQEDGQPGLIVCAVPYLRDRDIRRAEAGETFEDKGRKLVE
GIADHYCRVADAAAALNARLGGNIPIVAMGHLFTSGGTTLEGDGVRELYVGNLGQVTSDLLPDSFRYLALGHLHVAQRVN
GSSTRRYCGSPLPMSFGEANQRKLVLAVELDAAGLRVDELPVPCFQQLATLRGDWDHISRRIQELKRESAAIWLEVVYEG
GEIVGDLQERLRELVEGTSLEILRTRNMRLVDQALSAMATEETLDDLSVDDVFARCLDAHGVPDEQRPDLAALFAETMAA
LHEDDSRGAP

Sequences:

>Translated_410_residues
MKILHTSDWHIGRALYGRKRYEEFELFLDWLTTCIQSEQIEALLVAGDVFDNGTPSNRALELYYRFLCRAAGAGCRQVVV
TAGNHDSPSLLNAPRELLRHLDVHVVGCMTETVEDELVVVKQEDGQPGLIVCAVPYLRDRDIRRAEAGETFEDKGRKLVE
GIADHYCRVADAAAALNARLGGNIPIVAMGHLFTSGGTTLEGDGVRELYVGNLGQVTSDLLPDSFRYLALGHLHVAQRVN
GSSTRRYCGSPLPMSFGEANQRKLVLAVELDAAGLRVDELPVPCFQQLATLRGDWDHISRRIQELKRESAAIWLEVVYEG
GEIVGDLQERLRELVEGTSLEILRTRNMRLVDQALSAMATEETLDDLSVDDVFARCLDAHGVPDEQRPDLAALFAETMAA
LHEDDSRGAP
>Mature_410_residues
MKILHTSDWHIGRALYGRKRYEEFELFLDWLTTCIQSEQIEALLVAGDVFDNGTPSNRALELYYRFLCRAAGAGCRQVVV
TAGNHDSPSLLNAPRELLRHLDVHVVGCMTETVEDELVVVKQEDGQPGLIVCAVPYLRDRDIRRAEAGETFEDKGRKLVE
GIADHYCRVADAAAALNARLGGNIPIVAMGHLFTSGGTTLEGDGVRELYVGNLGQVTSDLLPDSFRYLALGHLHVAQRVN
GSSTRRYCGSPLPMSFGEANQRKLVLAVELDAAGLRVDELPVPCFQQLATLRGDWDHISRRIQELKRESAAIWLEVVYEG
GEIVGDLQERLRELVEGTSLEILRTRNMRLVDQALSAMATEETLDDLSVDDVFARCLDAHGVPDEQRPDLAALFAETMAA
LHEDDSRGAP

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=404, Percent_Identity=36.6336633663366, Blast_Score=263, Evalue=2e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 45346; Mature: 45346

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILHTSDWHIGRALYGRKRYEEFELFLDWLTTCIQSEQIEALLVAGDVFDNGTPSNRAL
CCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHH
ELYYRFLCRAAGAGCRQVVVTAGNHDSPSLLNAPRELLRHLDVHVVGCMTETVEDELVVV
HHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCHHEEEHHHHHCCCCEEEE
KQEDGQPGLIVCAVPYLRDRDIRRAEAGETFEDKGRKLVEGIADHYCRVADAAAALNARL
EECCCCCCEEEEECCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GGNIPIVAMGHLFTSGGTTLEGDGVRELYVGNLGQVTSDLLPDSFRYLALGHLHVAQRVN
CCCCCEEEECCEEECCCCEECCCCCCEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCC
GSSTRRYCGSPLPMSFGEANQRKLVLAVELDAAGLRVDELPVPCFQQLATLRGDWDHISR
CCCCHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCEECCCCCHHHHHHHHHCCCHHHHHH
RIQELKRESAAIWLEVVYEGGEIVGDLQERLRELVEGTSLEILRTRNMRLVDQALSAMAT
HHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHH
EETLDDLSVDDVFARCLDAHGVPDEQRPDLAALFAETMAALHEDDSRGAP
HHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKILHTSDWHIGRALYGRKRYEEFELFLDWLTTCIQSEQIEALLVAGDVFDNGTPSNRAL
CCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHH
ELYYRFLCRAAGAGCRQVVVTAGNHDSPSLLNAPRELLRHLDVHVVGCMTETVEDELVVV
HHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCHHEEEHHHHHCCCCEEEE
KQEDGQPGLIVCAVPYLRDRDIRRAEAGETFEDKGRKLVEGIADHYCRVADAAAALNARL
EECCCCCCEEEEECCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GGNIPIVAMGHLFTSGGTTLEGDGVRELYVGNLGQVTSDLLPDSFRYLALGHLHVAQRVN
CCCCCEEEECCEEECCCCEECCCCCCEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCC
GSSTRRYCGSPLPMSFGEANQRKLVLAVELDAAGLRVDELPVPCFQQLATLRGDWDHISR
CCCCHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCEECCCCCHHHHHHHHHCCCHHHHHH
RIQELKRESAAIWLEVVYEGGEIVGDLQERLRELVEGTSLEILRTRNMRLVDQALSAMAT
HHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHH
EETLDDLSVDDVFARCLDAHGVPDEQRPDLAALFAETMAALHEDDSRGAP
HHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]