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The map label for this gene is nucA [H]

Identifier: 118580977

GI number: 118580977

Start: 2778336

End: 2779139

Strand: Direct

Name: nucA [H]

Synonym: Ppro_2565

Alternate gene names: 118580977

Gene position: 2778336-2779139 (Clockwise)

Preceding gene: 118580973

Following gene: 118580978

Centisome position: 69.32

GC content: 61.44

Gene sequence:

>804_bases
ATGAAAGCACCGACAAAGGCTCTGGCTCTGCTGGCGCTGCTCGTTTCACTGACCATTTCCCCTGCTCTGGCTGGGCAGAC
AAGCTGCCCCGAACACTTTGCGGATGGCGAGGCCCCGGACCTGATCAACCAGAAACTCGACACAAAGACCCGTGAAGTAT
GCTATTCCGGTTATGCCATCAAACATTCCGGCATAACCAGGACGCCGCTGTACGCGGCCGAGCACCTGACCCGCCAACGG
CTCACCCGGGGCAAGGGGCTGAAACGCCACAACCGTTTCCATGCCGATGAAAACATACCGGAATCGGAACGGGCCGAACT
GCGCCACTACGCCCGCTCCGGGTACGACCGCGGCCACGTGGCCCCCTCGGCGGATATGTTCGACACGCAGTCCCAGTACG
AGTGCTTTTCACTGGCCAACATGATGCCCCAGATTCCGGAGAACAACCGCGGCCCCTGGGAGGGGATCGAATCCAGCGTG
AGGAAGATGGCGCGTGAACGGGGCGACCTGTATGTGGTCACCGGCCCGATCTTCCGGGGAGAGCGGGTGCAGCGCATCGG
CGGCGCGGTGCTGGTGCCTAGTAGCATGTTCAAGGCGGTCTACGACCCCACAAGGCAGGAGGCGGGCGCATACCTGATCG
ACAACAGCGCTGACAGCCAGCCGCAAATCATCTCCATAGCCGAGCTGGAGAAGGTGACCGGCATCAGCATCTTTCCGGCC
TTGAAACGCAAGGTCAAGGCCACGCCCATGAACCTGGCCGAGGCCGAGTCGTACCGGGAGCGTAAACAGAGGAGAGGAAT
ATGA

Upstream 100 bases:

>100_bases
CTTCCCCCACCAGAGCAGACAACAACAAAGAGCGGAAGCCGGGTTGACTCTCAGCTAAGGAATTGATACGGTTCGGGCTT
TTTGAGACCAAGGAGGTTTA

Downstream 100 bases:

>100_bases
CCAAGGCACAAACGATTAAACCGTTCGATAACGAGAGCGATTGTCTCCAGATGGGGGATCTGACCATCGAAAACCGGCTG
GACAGAGTCTCCCTCTTCGG

Product: DNA/RNA non-specific endonuclease

Products: NA

Alternate protein names: Endonuclease [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MKAPTKALALLALLVSLTISPALAGQTSCPEHFADGEAPDLINQKLDTKTREVCYSGYAIKHSGITRTPLYAAEHLTRQR
LTRGKGLKRHNRFHADENIPESERAELRHYARSGYDRGHVAPSADMFDTQSQYECFSLANMMPQIPENNRGPWEGIESSV
RKMARERGDLYVVTGPIFRGERVQRIGGAVLVPSSMFKAVYDPTRQEAGAYLIDNSADSQPQIISIAELEKVTGISIFPA
LKRKVKATPMNLAEAESYRERKQRRGI

Sequences:

>Translated_267_residues
MKAPTKALALLALLVSLTISPALAGQTSCPEHFADGEAPDLINQKLDTKTREVCYSGYAIKHSGITRTPLYAAEHLTRQR
LTRGKGLKRHNRFHADENIPESERAELRHYARSGYDRGHVAPSADMFDTQSQYECFSLANMMPQIPENNRGPWEGIESSV
RKMARERGDLYVVTGPIFRGERVQRIGGAVLVPSSMFKAVYDPTRQEAGAYLIDNSADSQPQIISIAELEKVTGISIFPA
LKRKVKATPMNLAEAESYRERKQRRGI
>Mature_267_residues
MKAPTKALALLALLVSLTISPALAGQTSCPEHFADGEAPDLINQKLDTKTREVCYSGYAIKHSGITRTPLYAAEHLTRQR
LTRGKGLKRHNRFHADENIPESERAELRHYARSGYDRGHVAPSADMFDTQSQYECFSLANMMPQIPENNRGPWEGIESSV
RKMARERGDLYVVTGPIFRGERVQRIGGAVLVPSSMFKAVYDPTRQEAGAYLIDNSADSQPQIISIAELEKVTGISIFPA
LKRKVKATPMNLAEAESYRERKQRRGI

Specific function: Catalyzes the degradation of both RNA and DNA; has the potential to act as an endonuclease [H]

COG id: COG1864

COG function: function code F; DNA/RNA endonuclease G, NUC1

Gene ontology:

Cell location: Periplasm. Note=Periplasmic or loosely attached to the cytoplasmic or the outer membrane [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA/RNA non-specific endonuclease family [H]

Homologues:

Organism=Homo sapiens, GI224451073, Length=220, Percent_Identity=32.2727272727273, Blast_Score=111, Evalue=7e-25,
Organism=Homo sapiens, GI224451075, Length=184, Percent_Identity=33.695652173913, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI53759134, Length=213, Percent_Identity=29.1079812206573, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17505967, Length=211, Percent_Identity=29.3838862559242, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6322253, Length=235, Percent_Identity=30.2127659574468, Blast_Score=96, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24581065, Length=230, Percent_Identity=27.8260869565217, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI20129899, Length=211, Percent_Identity=27.9620853080569, Blast_Score=84, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018524
- InterPro:   IPR001604
- InterPro:   IPR020821 [H]

Pfam domain/function: PF01223 Endonuclease_NS [H]

EC number: 3.1.30.-

Molecular weight: Translated: 29785; Mature: 29785

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAPTKALALLALLVSLTISPALAGQTSCPEHFADGEAPDLINQKLDTKTREVCYSGYAI
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCEE
KHSGITRTPLYAAEHLTRQRLTRGKGLKRHNRFHADENIPESERAELRHYARSGYDRGHV
ECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
APSADMFDTQSQYECFSLANMMPQIPENNRGPWEGIESSVRKMARERGDLYVVTGPIFRG
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCC
ERVQRIGGAVLVPSSMFKAVYDPTRQEAGAYLIDNSADSQPQIISIAELEKVTGISIFPA
HHHHHHCCEEECCHHHHHHHHCHHHHHCCEEEEECCCCCCCCEEEHHHHHHHCCCHHHHH
LKRKVKATPMNLAEAESYRERKQRRGI
HHHHHHCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKAPTKALALLALLVSLTISPALAGQTSCPEHFADGEAPDLINQKLDTKTREVCYSGYAI
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCEE
KHSGITRTPLYAAEHLTRQRLTRGKGLKRHNRFHADENIPESERAELRHYARSGYDRGHV
ECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
APSADMFDTQSQYECFSLANMMPQIPENNRGPWEGIESSVRKMARERGDLYVVTGPIFRG
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCC
ERVQRIGGAVLVPSSMFKAVYDPTRQEAGAYLIDNSADSQPQIISIAELEKVTGISIFPA
HHHHHHCCEEECCHHHHHHHHCHHHHHCCEEEEECCCCCCCCEEEHHHHHHHCCCHHHHH
LKRKVKATPMNLAEAESYRERKQRRGI
HHHHHHCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1343821; 11759840 [H]