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The map label for this gene is ilvI [H]

Identifier: 118580970

GI number: 118580970

Start: 2770026

End: 2771726

Strand: Reverse

Name: ilvI [H]

Synonym: Ppro_2558

Alternate gene names: 118580970

Gene position: 2771726-2770026 (Counterclockwise)

Preceding gene: 118580971

Following gene: 118580969

Centisome position: 69.15

GC content: 62.55

Gene sequence:

>1701_bases
ATGAAAATGAACGGCGCGCGTATCCTGCTGGAGTGCCTCATGAAGGAAGGGGTCGACACGGTCTTCGGCTACCCTGGTGG
CACGGTTCTCAATGTCTACGACGAACTCTTCGGTTGTAAAGAGATCCGCCACATCATGCCGCGGCACGAGCAGGCCGGCA
CCCATGCGGCCGACGGCTATGCCCGGGCAACCGGCAAGGTGGGTGTGGCCATCGCCACCTCCGGACCGGGGGCAACCAAC
ACGGTCACCGGCATCGCAACGGCCTACATGGATTCCATTCCCATGGTGATCATCACCGGTCAGGTGCCCACGGCCCTGAT
CGGCAACGATGCCTTCCAGGAGGTGGATATCGTCGGTATCACCCGTTCCTGTACCAAGCACAATTTCCTGGTCAAGGACG
TGAATGATATCGCCCCGATCATCAAGAAGGCCTTCTACATCGCACGCACCGGTCGTCCCGGTCCCGTGTTGGTGGACCTG
CCCAAGGACGTGCAGATCGCCAAGACCGAATTCTGCTACCCGGAGAGCGTGGAACTGCGCAGCTACAAGCCGACCACCGG
TGGCCACCCCCGCCAGGTGTCCAAGGCGGTGAGCATGCTTTTGGCTTCCCATCGTCCGGTGATGTACGTGGGCGGCGGGG
TGATCCTGAGCAATGCAGCCCCGGAGTTGACCGCCCTGTCCCGCCTGCTTTCCATTCCGGTCACCACCACCCTGATGGGG
TTGGGCGCCTTTCCGGGGGACGACGTCAATTCCCTGGGCATGCTGGGGATGCACGGCGCCTACTGCGCCAACATGGCCAT
GACCCACAGCGACCTGATCGTGGCCGTCGGCGCCCGTTTCGACGACCGGGTGACCGGCAAGGTGGATACCTTTGCCCCCC
ACGCCAAGATCATCCACATCGACGTGGACCCCACTTCCATCAGGAAGAACGTGCGGGTCGATCTCCCTATCGTGGGAGAT
GTGCGCGACGTGCTGACCAAGATGATCAAGAAGGTGGAAAAGTCCGAGGGAGATCAGGCCGCCTACCGGGAGTCCCTGGC
CCCCTGGCACGAACAGATCGCCGGCTGGAAAGAGAAACATCCGGTGTCATTCACCAGGAGCTCGGCGGTGATCAAGCCGC
AGTTCGTAATCCAGAAGTTGCGCGAGCTGTCCGACCGGGACGCCATCGTCTCCACCGACGTGGGGCAGCACCAGATGTGG
ACGGCCCAGTTCTTCAGCTTCACCGGCCCACGCACCCTGCTGACCTCCGGCGGGTTGGGCACCATGGGGTTCGGCCTGCC
GGCGGCCATGGGGGCCCAGGCAGCGTTCCCGGGACGCCAGGTGATCGCCATCTGCGGCGACGGTGGCATTCAGATGAACA
TCCAGGAGATGGCCACCCTGGTGCAGAACCGGCTGCCGGTGAAGATCGTGATCATCAACAACAACTTCCTCGGCATGGTG
CGCCAGTGGCAGGAGATGTTCTTCGACAAGCGCTACTCCCAATCCTGTCTGGAGCTGCCCATCGACTACATCAAGCTGGC
CGACGCCTACGGCGCCAAGGGGTTCGTGGCCTCAAAACCGTCCGAGGTGGAGACGGTCATCCGCCAGGGCTTTGCCGAAC
CAGGTCCGGTGATCATGGAGTTCAGGGTGGCACGGGAGGAGAAGGTGCTCCCCATGGTCCCCTCCGGGGCGTCGTTGAAC
GAGATGCTGCTGAACGCGTAA

Upstream 100 bases:

>100_bases
TGATGTTGCTCTGGTGTCAGACACGTTTTTCCCAGAGAACTCTGTGCCCTCTGTGGCTATGTACATTTTCCCGGTTCATG
TTTGCGGATGGAGTGATGGT

Downstream 100 bases:

>100_bases
GCTCACCGTTTCTCCCCTTCACTTTTTTTCCGTCGAGGCGTATCCCATGCGACATACTATTTCCGTCCTGGTCGAAAACG
AGTTTGGCGTTGTCTCCCGT

Product: acetolactate synthase, large subunit, biosynthetic type

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 566; Mature: 566

Protein sequence:

>566_residues
MKMNGARILLECLMKEGVDTVFGYPGGTVLNVYDELFGCKEIRHIMPRHEQAGTHAADGYARATGKVGVAIATSGPGATN
TVTGIATAYMDSIPMVIITGQVPTALIGNDAFQEVDIVGITRSCTKHNFLVKDVNDIAPIIKKAFYIARTGRPGPVLVDL
PKDVQIAKTEFCYPESVELRSYKPTTGGHPRQVSKAVSMLLASHRPVMYVGGGVILSNAAPELTALSRLLSIPVTTTLMG
LGAFPGDDVNSLGMLGMHGAYCANMAMTHSDLIVAVGARFDDRVTGKVDTFAPHAKIIHIDVDPTSIRKNVRVDLPIVGD
VRDVLTKMIKKVEKSEGDQAAYRESLAPWHEQIAGWKEKHPVSFTRSSAVIKPQFVIQKLRELSDRDAIVSTDVGQHQMW
TAQFFSFTGPRTLLTSGGLGTMGFGLPAAMGAQAAFPGRQVIAICGDGGIQMNIQEMATLVQNRLPVKIVIINNNFLGMV
RQWQEMFFDKRYSQSCLELPIDYIKLADAYGAKGFVASKPSEVETVIRQGFAEPGPVIMEFRVAREEKVLPMVPSGASLN
EMLLNA

Sequences:

>Translated_566_residues
MKMNGARILLECLMKEGVDTVFGYPGGTVLNVYDELFGCKEIRHIMPRHEQAGTHAADGYARATGKVGVAIATSGPGATN
TVTGIATAYMDSIPMVIITGQVPTALIGNDAFQEVDIVGITRSCTKHNFLVKDVNDIAPIIKKAFYIARTGRPGPVLVDL
PKDVQIAKTEFCYPESVELRSYKPTTGGHPRQVSKAVSMLLASHRPVMYVGGGVILSNAAPELTALSRLLSIPVTTTLMG
LGAFPGDDVNSLGMLGMHGAYCANMAMTHSDLIVAVGARFDDRVTGKVDTFAPHAKIIHIDVDPTSIRKNVRVDLPIVGD
VRDVLTKMIKKVEKSEGDQAAYRESLAPWHEQIAGWKEKHPVSFTRSSAVIKPQFVIQKLRELSDRDAIVSTDVGQHQMW
TAQFFSFTGPRTLLTSGGLGTMGFGLPAAMGAQAAFPGRQVIAICGDGGIQMNIQEMATLVQNRLPVKIVIINNNFLGMV
RQWQEMFFDKRYSQSCLELPIDYIKLADAYGAKGFVASKPSEVETVIRQGFAEPGPVIMEFRVAREEKVLPMVPSGASLN
EMLLNA
>Mature_566_residues
MKMNGARILLECLMKEGVDTVFGYPGGTVLNVYDELFGCKEIRHIMPRHEQAGTHAADGYARATGKVGVAIATSGPGATN
TVTGIATAYMDSIPMVIITGQVPTALIGNDAFQEVDIVGITRSCTKHNFLVKDVNDIAPIIKKAFYIARTGRPGPVLVDL
PKDVQIAKTEFCYPESVELRSYKPTTGGHPRQVSKAVSMLLASHRPVMYVGGGVILSNAAPELTALSRLLSIPVTTTLMG
LGAFPGDDVNSLGMLGMHGAYCANMAMTHSDLIVAVGARFDDRVTGKVDTFAPHAKIIHIDVDPTSIRKNVRVDLPIVGD
VRDVLTKMIKKVEKSEGDQAAYRESLAPWHEQIAGWKEKHPVSFTRSSAVIKPQFVIQKLRELSDRDAIVSTDVGQHQMW
TAQFFSFTGPRTLLTSGGLGTMGFGLPAAMGAQAAFPGRQVIAICGDGGIQMNIQEMATLVQNRLPVKIVIINNNFLGMV
RQWQEMFFDKRYSQSCLELPIDYIKLADAYGAKGFVASKPSEVETVIRQGFAEPGPVIMEFRVAREEKVLPMVPSGASLN
EMLLNA

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=546, Percent_Identity=24.7252747252747, Blast_Score=162, Evalue=8e-40,
Organism=Homo sapiens, GI21361361, Length=597, Percent_Identity=26.1306532663317, Blast_Score=160, Evalue=4e-39,
Organism=Escherichia coli, GI87081685, Length=568, Percent_Identity=49.1197183098592, Blast_Score=578, Evalue=1e-166,
Organism=Escherichia coli, GI1790104, Length=563, Percent_Identity=42.6287744227353, Blast_Score=456, Evalue=1e-129,
Organism=Escherichia coli, GI1786717, Length=557, Percent_Identity=35.9066427289048, Blast_Score=325, Evalue=3e-90,
Organism=Escherichia coli, GI1787096, Length=555, Percent_Identity=29.1891891891892, Blast_Score=227, Evalue=2e-60,
Organism=Escherichia coli, GI1788716, Length=550, Percent_Identity=25.6363636363636, Blast_Score=152, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI17531299, Length=589, Percent_Identity=26.3157894736842, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17531301, Length=578, Percent_Identity=26.643598615917, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17542570, Length=529, Percent_Identity=25.8979206049149, Blast_Score=137, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6323755, Length=587, Percent_Identity=45.6558773424191, Blast_Score=492, Evalue=1e-140,
Organism=Saccharomyces cerevisiae, GI6320816, Length=576, Percent_Identity=24.6527777777778, Blast_Score=103, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6320123, Length=545, Percent_Identity=22.2018348623853, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323163, Length=498, Percent_Identity=24.0963855421687, Blast_Score=87, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6321524, Length=536, Percent_Identity=21.8283582089552, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323073, Length=492, Percent_Identity=22.9674796747967, Blast_Score=80, Evalue=8e-16,
Organism=Drosophila melanogaster, GI19922626, Length=551, Percent_Identity=24.3194192377495, Blast_Score=156, Evalue=3e-38,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61347; Mature: 61347

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMNGARILLECLMKEGVDTVFGYPGGTVLNVYDELFGCKEIRHIMPRHEQAGTHAADGY
CCCCHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCHHHHHHHCCCCHHCCCCCCCCH
ARATGKVGVAIATSGPGATNTVTGIATAYMDSIPMVIITGQVPTALIGNDAFQEVDIVGI
HCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCEECCCCCHHEEEEEEE
TRSCTKHNFLVKDVNDIAPIIKKAFYIARTGRPGPVLVDLPKDVQIAKTEFCYPESVELR
ECCCCCCCCEEHHHHHHHHHHHHHHHEEECCCCCCEEEECCCCCEEEHHHCCCCCCCCCE
SYKPTTGGHPRQVSKAVSMLLASHRPVMYVGGGVILSNAAPELTALSRLLSIPVTTTLMG
EECCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEEECCCCCHHHHHHHHHHCCHHHHHHH
LGAFPGDDVNSLGMLGMHGAYCANMAMTHSDLIVAVGARFDDRVTGKVDTFAPHAKIIHI
CCCCCCCCCCCCCCCCCCHHHHHHHHEECCCEEEEECCCCCCCCCCCCCCCCCCEEEEEE
DVDPTSIRKNVRVDLPIVGDVRDVLTKMIKKVEKSEGDQAAYRESLAPWHEQIAGWKEKH
ECCHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHCCCCCCC
PVSFTRSSAVIKPQFVIQKLRELSDRDAIVSTDVGQHQMWTAQFFSFTGPRTLLTSGGLG
CCCEECCCCEECHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCCCCCEEECCCCC
TMGFGLPAAMGAQAAFPGRQVIAICGDGGIQMNIQEMATLVQNRLPVKIVIINNNFLGMV
CCCCCCHHHHCCCCCCCCCEEEEEECCCCCEEEHHHHHHHHHCCCCEEEEEECCCHHHHH
RQWQEMFFDKRYSQSCLELPIDYIKLADAYGAKGFVASKPSEVETVIRQGFAEPGPVIME
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEECCCCHHHHHHHHHCCCCCCCEEEE
FRVAREEKVLPMVPSGASLNEMLLNA
EEECCCCCCCEECCCCCCHHHHHHCC
>Mature Secondary Structure
MKMNGARILLECLMKEGVDTVFGYPGGTVLNVYDELFGCKEIRHIMPRHEQAGTHAADGY
CCCCHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCHHHHHHHCCCCHHCCCCCCCCH
ARATGKVGVAIATSGPGATNTVTGIATAYMDSIPMVIITGQVPTALIGNDAFQEVDIVGI
HCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCEECCCCCHHEEEEEEE
TRSCTKHNFLVKDVNDIAPIIKKAFYIARTGRPGPVLVDLPKDVQIAKTEFCYPESVELR
ECCCCCCCCEEHHHHHHHHHHHHHHHEEECCCCCCEEEECCCCCEEEHHHCCCCCCCCCE
SYKPTTGGHPRQVSKAVSMLLASHRPVMYVGGGVILSNAAPELTALSRLLSIPVTTTLMG
EECCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEEECCCCCHHHHHHHHHHCCHHHHHHH
LGAFPGDDVNSLGMLGMHGAYCANMAMTHSDLIVAVGARFDDRVTGKVDTFAPHAKIIHI
CCCCCCCCCCCCCCCCCCHHHHHHHHEECCCEEEEECCCCCCCCCCCCCCCCCCEEEEEE
DVDPTSIRKNVRVDLPIVGDVRDVLTKMIKKVEKSEGDQAAYRESLAPWHEQIAGWKEKH
ECCHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHCCCCCCC
PVSFTRSSAVIKPQFVIQKLRELSDRDAIVSTDVGQHQMWTAQFFSFTGPRTLLTSGGLG
CCCEECCCCEECHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCCCCCEEECCCCC
TMGFGLPAAMGAQAAFPGRQVIAICGDGGIQMNIQEMATLVQNRLPVKIVIINNNFLGMV
CCCCCCHHHHCCCCCCCCCEEEEEECCCCCEEEHHHHHHHHHCCCCEEEEEECCCHHHHH
RQWQEMFFDKRYSQSCLELPIDYIKLADAYGAKGFVASKPSEVETVIRQGFAEPGPVIME
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEECCCCHHHHHHHHHCCCCCCCEEEE
FRVAREEKVLPMVPSGASLNEMLLNA
EEECCCCCCCEECCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9688822; 12089438 [H]