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The map label for this gene is pcrA [H]

Identifier: 118580949

GI number: 118580949

Start: 2748868

End: 2750886

Strand: Direct

Name: pcrA [H]

Synonym: Ppro_2537

Alternate gene names: 118580949

Gene position: 2748868-2750886 (Clockwise)

Preceding gene: 118580919

Following gene: 118580951

Centisome position: 68.58

GC content: 63.2

Gene sequence:

>2019_bases
ATGAAGAAACTCAACCCGCAACAGTTCCTGGCAGTGCGCCACACCGAGGGGCCGCTCCTGATCCTGGCCGGGGCAGGCTC
CGGCAAGACCCAGGTCATCACCACCCGCATCGTCCACCTGATCAAGGACAGACAGGTCCCGGCGGAGAATATCCTGGCGG
TGACCTTCACCAACAAGGCGGCCCGCGAGATGCGCGAACGGGTCGCCTCCATGGCCGGTAAAAGTTCCGAGGGGATCATC
ATCTCCACCTTCCACTCCCTGGGGGTGCGTATCCTCAGGCGCGACATCCGGTCACTGGGATTCAAACCCAACTTCTCCAT
CTACTCATCCTCGGACCAGGCCGGGGTCCTGCGCCAGGCCATGCGCGAGCGCAACATCGACCCCAAGCAGTGCGACCCGG
ACAGCATCCTCTGGCGCATCTCCGGCGCCAAGAACCGGCTGGTAGGGCCCTACGAGTTCAAACCGACCAGTGACGACCGC
ATCGAGCTGGCCACGGCCAGCGTCTACCCCCGCTACCAGGAGCTGCTGAAAGGCTTCAACGCCATCGACTTCGACGACAT
CATCATGCTGTCGGTCAAGCTGCTGCAGTCCACCCCGGCCATCCTGGAGCACTGGCAGAAGCGCTTCAGCCACATCATGG
TGGACGAGTACCAGGACACCAACGCCTCCCAGTACCTGCTCATCTCGCTCCTGGCAAAGCTGCACGGCAACCTGTGCGTG
GTGGGGGACGACGACCAGTCCATCTACGGCTGGCGCGGGGCCGAGGTGCGCAACATCCTCAACTTCGAGCATGACCACGC
CGGCTGCCGGGTGATCAAGCTGGAGCAGAACTACCGCTCCACCAGCTCCATCCTGGATGCCGCCAACAGCGTCATCAAAA
ACAACCAGCTGCGCACGGACAAGGCGCTCTGGACCGCCCTGGGCAAGGGCCGCGAGATCGACCTGATCGTGGCCGGGAGC
GAGGAGGACGAGGCGGCCAAGGTGGTGGAGAGCATGCTGATGGAGCAGTTCCGCGACAAGCTCTCTTGGTCGGACCTGGC
CATCCTCTACCGCTCCAACTCCCAGAGCCGGGCCTTCGAGGAGAAGCTGCGCCAGGAGCGCATCCCCTACGTGGTCATCG
GCGGCCAGCAGTTCTACGAGCGCAAGGAGGTCAAGGACGCCATCGCCTACCTGAAGGTGATCGACAACCCCAGCGACGAG
GCGTCGCTCCTACGCATCATCAACTTCCCCCGCCGGGGGATCGGCGACAACACGCTGGTGCGGCTGAACCAGTGGTCCCT
GGAGCACGACCTGCCGTTGTTCCAGACATTGGCACGGGTGCGGGATATCCCGGACATATCCGAGTCGTCCAAGAAGGCGG
TGGAGGCGTTCCACGCCATGATGCGGGGGGTAATGGAGGGGTTCAAAAATATCCGCATGGGGGAGCAGGTCAATGCCCTG
TTCAACCGGCTGCGCATCGAGGATGAGCTGTACCGCACCCTGAACGACGCAAGCCAGGCCCGAAAGCGCATCGAGAACAT
CGAGCAGGTGGTCAACTCCCTGGCCGTGTACGAGGAGCAGAATCCCAAGGGCACCCTGTCCGCTTTCCTGGAACGCATCT
CGCTTTTGGACGAGGACAAACCAAACGAGGACGACAAGGAGCATGGTCGCGACGCGGTCACGCTCATGTCGCTGCACTCC
AGCAAGGGGCTGGAGTTCAGCCACGTCTTCCTGGTGGGGATGGAGGAGGACCTGCTGCCCCACAAGCGCTCCATCGAGGA
GGACCCGACGGTGGCCGAGGAGCGGCGCCTCTGCTACGTGGGCATCACCCGCGCCCGGCGCCACCTGACCATCTCCCGCT
GCCGCACGCGCAGGAAGTACGGGGCGGTGGAACAGCGCAACCCCAGCCGCTTCCTGGACGAAATACCGGAGCATCTTTTG
AACAGCGAGGGGGGCGAACAGCAGGAGGGGGCAAAGGATTCAGACTCCATGGCAGCCGACTTCTTCGCCCAGATGCTGGG
GGAAAACAGTGATCAGTGA

Upstream 100 bases:

>100_bases
CATCCGTTCCTCCACCTTTCTGCATCACTTCCCTGATCTTACACCCTGCCCAAACAAAATTTGCCCGCCCTCCCCCCTTT
GTGCTACACATTGTCCCCCT

Downstream 100 bases:

>100_bases
TCAGTGATCAGTGATCAGGGTACAGGGTACAGTAAAACCGCTGTCCACTGTCCACTGCCTACATCCTCTCGTACGGTTCG
AACAGGCGGCGGTATTCGTC

Product: UvrD/REP helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 672; Mature: 672

Protein sequence:

>672_residues
MKKLNPQQFLAVRHTEGPLLILAGAGSGKTQVITTRIVHLIKDRQVPAENILAVTFTNKAAREMRERVASMAGKSSEGII
ISTFHSLGVRILRRDIRSLGFKPNFSIYSSSDQAGVLRQAMRERNIDPKQCDPDSILWRISGAKNRLVGPYEFKPTSDDR
IELATASVYPRYQELLKGFNAIDFDDIIMLSVKLLQSTPAILEHWQKRFSHIMVDEYQDTNASQYLLISLLAKLHGNLCV
VGDDDQSIYGWRGAEVRNILNFEHDHAGCRVIKLEQNYRSTSSILDAANSVIKNNQLRTDKALWTALGKGREIDLIVAGS
EEDEAAKVVESMLMEQFRDKLSWSDLAILYRSNSQSRAFEEKLRQERIPYVVIGGQQFYERKEVKDAIAYLKVIDNPSDE
ASLLRIINFPRRGIGDNTLVRLNQWSLEHDLPLFQTLARVRDIPDISESSKKAVEAFHAMMRGVMEGFKNIRMGEQVNAL
FNRLRIEDELYRTLNDASQARKRIENIEQVVNSLAVYEEQNPKGTLSAFLERISLLDEDKPNEDDKEHGRDAVTLMSLHS
SKGLEFSHVFLVGMEEDLLPHKRSIEEDPTVAEERRLCYVGITRARRHLTISRCRTRRKYGAVEQRNPSRFLDEIPEHLL
NSEGGEQQEGAKDSDSMAADFFAQMLGENSDQ

Sequences:

>Translated_672_residues
MKKLNPQQFLAVRHTEGPLLILAGAGSGKTQVITTRIVHLIKDRQVPAENILAVTFTNKAAREMRERVASMAGKSSEGII
ISTFHSLGVRILRRDIRSLGFKPNFSIYSSSDQAGVLRQAMRERNIDPKQCDPDSILWRISGAKNRLVGPYEFKPTSDDR
IELATASVYPRYQELLKGFNAIDFDDIIMLSVKLLQSTPAILEHWQKRFSHIMVDEYQDTNASQYLLISLLAKLHGNLCV
VGDDDQSIYGWRGAEVRNILNFEHDHAGCRVIKLEQNYRSTSSILDAANSVIKNNQLRTDKALWTALGKGREIDLIVAGS
EEDEAAKVVESMLMEQFRDKLSWSDLAILYRSNSQSRAFEEKLRQERIPYVVIGGQQFYERKEVKDAIAYLKVIDNPSDE
ASLLRIINFPRRGIGDNTLVRLNQWSLEHDLPLFQTLARVRDIPDISESSKKAVEAFHAMMRGVMEGFKNIRMGEQVNAL
FNRLRIEDELYRTLNDASQARKRIENIEQVVNSLAVYEEQNPKGTLSAFLERISLLDEDKPNEDDKEHGRDAVTLMSLHS
SKGLEFSHVFLVGMEEDLLPHKRSIEEDPTVAEERRLCYVGITRARRHLTISRCRTRRKYGAVEQRNPSRFLDEIPEHLL
NSEGGEQQEGAKDSDSMAADFFAQMLGENSDQ
>Mature_672_residues
MKKLNPQQFLAVRHTEGPLLILAGAGSGKTQVITTRIVHLIKDRQVPAENILAVTFTNKAAREMRERVASMAGKSSEGII
ISTFHSLGVRILRRDIRSLGFKPNFSIYSSSDQAGVLRQAMRERNIDPKQCDPDSILWRISGAKNRLVGPYEFKPTSDDR
IELATASVYPRYQELLKGFNAIDFDDIIMLSVKLLQSTPAILEHWQKRFSHIMVDEYQDTNASQYLLISLLAKLHGNLCV
VGDDDQSIYGWRGAEVRNILNFEHDHAGCRVIKLEQNYRSTSSILDAANSVIKNNQLRTDKALWTALGKGREIDLIVAGS
EEDEAAKVVESMLMEQFRDKLSWSDLAILYRSNSQSRAFEEKLRQERIPYVVIGGQQFYERKEVKDAIAYLKVIDNPSDE
ASLLRIINFPRRGIGDNTLVRLNQWSLEHDLPLFQTLARVRDIPDISESSKKAVEAFHAMMRGVMEGFKNIRMGEQVNAL
FNRLRIEDELYRTLNDASQARKRIENIEQVVNSLAVYEEQNPKGTLSAFLERISLLDEDKPNEDDKEHGRDAVTLMSLHS
SKGLEFSHVFLVGMEEDLLPHKRSIEEDPTVAEERRLCYVGITRARRHLTISRCRTRRKYGAVEQRNPSRFLDEIPEHLL
NSEGGEQQEGAKDSDSMAADFFAQMLGENSDQ

Specific function: Essential helicase [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=644, Percent_Identity=41.9254658385093, Blast_Score=474, Evalue=1e-134,
Organism=Escherichia coli, GI2367296, Length=646, Percent_Identity=40.0928792569659, Blast_Score=453, Evalue=1e-128,
Organism=Escherichia coli, GI1787196, Length=342, Percent_Identity=28.0701754385965, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6322369, Length=746, Percent_Identity=27.3458445040214, Blast_Score=201, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6324477, Length=679, Percent_Identity=22.9749631811487, Blast_Score=126, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 76506; Mature: 76506

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLNPQQFLAVRHTEGPLLILAGAGSGKTQVITTRIVHLIKDRQVPAENILAVTFTNKA
CCCCCHHHEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCHHHEEEEEECCHH
AREMRERVASMAGKSSEGIIISTFHSLGVRILRRDIRSLGFKPNFSIYSSSDQAGVLRQA
HHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHH
MRERNIDPKQCDPDSILWRISGAKNRLVGPYEFKPTSDDRIELATASVYPRYQELLKGFN
HHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEHHCCHHHHHHHHCCC
AIDFDDIIMLSVKLLQSTPAILEHWQKRFSHIMVDEYQDTNASQYLLISLLAKLHGNLCV
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEE
VGDDDQSIYGWRGAEVRNILNFEHDHAGCRVIKLEQNYRSTSSILDAANSVIKNNQLRTD
EECCCCCCCCCCCHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCHH
KALWTALGKGREIDLIVAGSEEDEAAKVVESMLMEQFRDKLSWSDLAILYRSNSQSRAFE
HHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHH
EKLRQERIPYVVIGGQQFYERKEVKDAIAYLKVIDNPSDEASLLRIINFPRRGIGDNTLV
HHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCEE
RLNQWSLEHDLPLFQTLARVRDIPDISESSKKAVEAFHAMMRGVMEGFKNIRMGEQVNAL
EEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
FNRLRIEDELYRTLNDASQARKRIENIEQVVNSLAVYEEQNPKGTLSAFLERISLLDEDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCC
PNEDDKEHGRDAVTLMSLHSSKGLEFSHVFLVGMEEDLLPHKRSIEEDPTVAEERRLCYV
CCCCHHHHCCHHEEHEEHHCCCCCCEEEEEEEECCHHCCCCHHCCCCCCCHHHHCCEEEE
GITRARRHLTISRCRTRRKYGAVEQRNPSRFLDEIPEHLLNSEGGEQQEGAKDSDSMAAD
EHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
FFAQMLGENSDQ
HHHHHHCCCCCC
>Mature Secondary Structure
MKKLNPQQFLAVRHTEGPLLILAGAGSGKTQVITTRIVHLIKDRQVPAENILAVTFTNKA
CCCCCHHHEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCHHHEEEEEECCHH
AREMRERVASMAGKSSEGIIISTFHSLGVRILRRDIRSLGFKPNFSIYSSSDQAGVLRQA
HHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHH
MRERNIDPKQCDPDSILWRISGAKNRLVGPYEFKPTSDDRIELATASVYPRYQELLKGFN
HHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEHHCCHHHHHHHHCCC
AIDFDDIIMLSVKLLQSTPAILEHWQKRFSHIMVDEYQDTNASQYLLISLLAKLHGNLCV
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEE
VGDDDQSIYGWRGAEVRNILNFEHDHAGCRVIKLEQNYRSTSSILDAANSVIKNNQLRTD
EECCCCCCCCCCCHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCHH
KALWTALGKGREIDLIVAGSEEDEAAKVVESMLMEQFRDKLSWSDLAILYRSNSQSRAFE
HHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHH
EKLRQERIPYVVIGGQQFYERKEVKDAIAYLKVIDNPSDEASLLRIINFPRRGIGDNTLV
HHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCEE
RLNQWSLEHDLPLFQTLARVRDIPDISESSKKAVEAFHAMMRGVMEGFKNIRMGEQVNAL
EEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
FNRLRIEDELYRTLNDASQARKRIENIEQVVNSLAVYEEQNPKGTLSAFLERISLLDEDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCC
PNEDDKEHGRDAVTLMSLHSSKGLEFSHVFLVGMEEDLLPHKRSIEEDPTVAEERRLCYV
CCCCHHHHCCHHEEHEEHHCCCCCCEEEEEEEECCHHCCCCHHCCCCCCCHHHHCCEEEE
GITRARRHLTISRCRTRRKYGAVEQRNPSRFLDEIPEHLLNSEGGEQQEGAKDSDSMAAD
EHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
FFAQMLGENSDQ
HHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA