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The map label for this gene is carA [H]

Identifier: 118580939

GI number: 118580939

Start: 2738910

End: 2740031

Strand: Reverse

Name: carA [H]

Synonym: Ppro_2527

Alternate gene names: 118580939

Gene position: 2740031-2738910 (Counterclockwise)

Preceding gene: 118580940

Following gene: 118580938

Centisome position: 68.36

GC content: 63.37

Gene sequence:

>1122_bases
ATGAAAGCATTACTGGCGCTCGCCGACGGGCGCATCTTTGAGGGGAAATCATTCGGAGCGGGAGGCGAGGCCCGCGGAGA
GGTGGTGTTCAACACCGCCATGACCGGCTACCAGGAGGTGTTGACCGACCCCTCCTACAAGGGGCAGATGGTCACCATGA
CCTACACCCAGATCGGTAACACCGGTATCAATGCCGAGGATATCGAGAGCCGCGGCCTGTTCCTCTCCGGCTTCATCGTC
CGCGAGTACCAGGAGTGCTACTCCAACTGGCGGGCCTGCATGAGCCTGGACTCCTACCTCAAGGAGAACGGCGTGATCGG
CATCCAGGGGATCGACACCCGGGCGCTCACCCGCCACCTGCGGGACAAGGGTGCCCAGAACGGCATCATCTCCACCATTG
ACCTGGACCGGGAGAGCCTGGTGGCCAAGGCGCGGGCCATCCCCAGCATGGCCGGCCTGGACCTGGCCACGGGCGTCTCC
TGCGACAAACCCTATCACTGGACCGAGGGGCTCTGGGACCTGAAGGACGGCTATCCCCAGGTGGATCCCGTTACGCTCAA
GTACAAGGTGGTGGCCTACGATTTCGGCATCAAGTACAACATCCTCCGCTGCCTGGTGGACGCCGGCTGCGACGTGACCG
TGGTGCCAGCCTCCTTCCCGGCGGAAGAGGCGCTGGCCATGAACCCGGACGGTGTCTTCCTCTCCAACGGTCCCGGTGAC
CCGGAACCGTTGGTGGCGGTGCAGGAGGAGATCAGGAAGATGATCGGCAGGAAACCGATCTTCGGTATCTGCCTGGGGCA
TCAGCTCTTGGGGCTGGCCCTGGGTGGCAAGACCATGAAGCTCCCCTTCGGCAACCACGGCTCCAACCTGCCGGTCATGG
ACATGGCCACCCGCAAGGTAGAGATCACCTCCCAGAACCACGGCTTTGCCGTTGACCTGGATTCCCTGGGGGATGCGGCC
TGCCTGGGGCACGAGAACCTGAACGACCAGACCGTGGAGGGAATCAGCCACTGCACGCTTCCGATCTTCTCGGTGCAGCA
CCACCCCGAGGCCTCTCCCGGCCCCCACGACTCCCACTATCTGTTCGGCCGCTTCGTGGAGATGATGGAACGAAACCGGT
GA

Upstream 100 bases:

>100_bases
GAACGCAGGATGATGAAGAGCGGTTTTCTTCGTGCGCTTCGTGCCTTCGTGTGAGTCTGCTGTTTTTCGGATTTTACAGA
ATTTCTTTGGAGTCACTATT

Downstream 100 bases:

>100_bases
GGAGCGAGGAGTGAAGAGTGAGGGCCGACGTAACGCATGATCCGACAAACTGCTCCCCGGTTCCCGGTTCCCCGTCCTAC
CTGGAACTCTTCCATTCCGG

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MKALLALADGRIFEGKSFGAGGEARGEVVFNTAMTGYQEVLTDPSYKGQMVTMTYTQIGNTGINAEDIESRGLFLSGFIV
REYQECYSNWRACMSLDSYLKENGVIGIQGIDTRALTRHLRDKGAQNGIISTIDLDRESLVAKARAIPSMAGLDLATGVS
CDKPYHWTEGLWDLKDGYPQVDPVTLKYKVVAYDFGIKYNILRCLVDAGCDVTVVPASFPAEEALAMNPDGVFLSNGPGD
PEPLVAVQEEIRKMIGRKPIFGICLGHQLLGLALGGKTMKLPFGNHGSNLPVMDMATRKVEITSQNHGFAVDLDSLGDAA
CLGHENLNDQTVEGISHCTLPIFSVQHHPEASPGPHDSHYLFGRFVEMMERNR

Sequences:

>Translated_373_residues
MKALLALADGRIFEGKSFGAGGEARGEVVFNTAMTGYQEVLTDPSYKGQMVTMTYTQIGNTGINAEDIESRGLFLSGFIV
REYQECYSNWRACMSLDSYLKENGVIGIQGIDTRALTRHLRDKGAQNGIISTIDLDRESLVAKARAIPSMAGLDLATGVS
CDKPYHWTEGLWDLKDGYPQVDPVTLKYKVVAYDFGIKYNILRCLVDAGCDVTVVPASFPAEEALAMNPDGVFLSNGPGD
PEPLVAVQEEIRKMIGRKPIFGICLGHQLLGLALGGKTMKLPFGNHGSNLPVMDMATRKVEITSQNHGFAVDLDSLGDAA
CLGHENLNDQTVEGISHCTLPIFSVQHHPEASPGPHDSHYLFGRFVEMMERNR
>Mature_373_residues
MKALLALADGRIFEGKSFGAGGEARGEVVFNTAMTGYQEVLTDPSYKGQMVTMTYTQIGNTGINAEDIESRGLFLSGFIV
REYQECYSNWRACMSLDSYLKENGVIGIQGIDTRALTRHLRDKGAQNGIISTIDLDRESLVAKARAIPSMAGLDLATGVS
CDKPYHWTEGLWDLKDGYPQVDPVTLKYKVVAYDFGIKYNILRCLVDAGCDVTVVPASFPAEEALAMNPDGVFLSNGPGD
PEPLVAVQEEIRKMIGRKPIFGICLGHQLLGLALGGKTMKLPFGNHGSNLPVMDMATRKVEITSQNHGFAVDLDSLGDAA
CLGHENLNDQTVEGISHCTLPIFSVQHHPEASPGPHDSHYLFGRFVEMMERNR

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=386, Percent_Identity=40.1554404145078, Blast_Score=255, Evalue=5e-68,
Organism=Homo sapiens, GI21361331, Length=391, Percent_Identity=36.8286445012788, Blast_Score=233, Evalue=3e-61,
Organism=Homo sapiens, GI169790915, Length=391, Percent_Identity=36.8286445012788, Blast_Score=233, Evalue=3e-61,
Organism=Escherichia coli, GI1786215, Length=377, Percent_Identity=52.2546419098143, Blast_Score=376, Evalue=1e-105,
Organism=Escherichia coli, GI1789760, Length=183, Percent_Identity=27.8688524590164, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI193204318, Length=389, Percent_Identity=38.8174807197943, Blast_Score=240, Evalue=9e-64,
Organism=Saccharomyces cerevisiae, GI6324878, Length=386, Percent_Identity=40.4145077720207, Blast_Score=266, Evalue=5e-72,
Organism=Saccharomyces cerevisiae, GI6322331, Length=389, Percent_Identity=37.7892030848329, Blast_Score=261, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24642586, Length=388, Percent_Identity=40.2061855670103, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI45555749, Length=388, Percent_Identity=40.2061855670103, Blast_Score=253, Evalue=1e-67,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 40691; Mature: 40691

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKALLALADGRIFEGKSFGAGGEARGEVVFNTAMTGYQEVLTDPSYKGQMVTMTYTQIGN
CCCEEEECCCEEECCCCCCCCCCCCCCEEEECHHHHHHHHHCCCCCCCEEEEEEEEECCC
TGINAEDIESRGLFLSGFIVREYQECYSNWRACMSLDSYLKENGVIGIQGIDTRALTRHL
CCCCHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHH
RDKGAQNGIISTIDLDRESLVAKARAIPSMAGLDLATGVSCDKPYHWTEGLWDLKDGYPQ
HHCCCCCCCEEEEECCHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCEECCCCCCC
VDPVTLKYKVVAYDFGIKYNILRCLVDAGCDVTVVPASFPAEEALAMNPDGVFLSNGPGD
CCCEEEEEEEEEEECCCHHHHHHHHHHCCCCEEEEECCCCCCHHEEECCCCEEECCCCCC
PEPLVAVQEEIRKMIGRKPIFGICLGHQLLGLALGGKTMKLPFGNHGSNLPVMDMATRKV
CCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHEECCCEEECCCCCCCCCCCEEECCCEEE
EITSQNHGFAVDLDSLGDAACLGHENLNDQTVEGISHCTLPIFSVQHHPEASPGPHDSHY
EEEECCCCEEEECCCCCCEEEECCCCCCHHHHHHHHHCCEEEEEECCCCCCCCCCCCCHH
LFGRFVEMMERNR
HHHHHHHHHHCCC
>Mature Secondary Structure
MKALLALADGRIFEGKSFGAGGEARGEVVFNTAMTGYQEVLTDPSYKGQMVTMTYTQIGN
CCCEEEECCCEEECCCCCCCCCCCCCCEEEECHHHHHHHHHCCCCCCCEEEEEEEEECCC
TGINAEDIESRGLFLSGFIVREYQECYSNWRACMSLDSYLKENGVIGIQGIDTRALTRHL
CCCCHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHH
RDKGAQNGIISTIDLDRESLVAKARAIPSMAGLDLATGVSCDKPYHWTEGLWDLKDGYPQ
HHCCCCCCCEEEEECCHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCEECCCCCCC
VDPVTLKYKVVAYDFGIKYNILRCLVDAGCDVTVVPASFPAEEALAMNPDGVFLSNGPGD
CCCEEEEEEEEEEECCCHHHHHHHHHHCCCCEEEEECCCCCCHHEEECCCCEEECCCCCC
PEPLVAVQEEIRKMIGRKPIFGICLGHQLLGLALGGKTMKLPFGNHGSNLPVMDMATRKV
CCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHEECCCEEECCCCCCCCCCCEEECCCEEE
EITSQNHGFAVDLDSLGDAACLGHENLNDQTVEGISHCTLPIFSVQHHPEASPGPHDSHY
EEEECCCCEEEECCCCCCEEEECCCCCCHHHHHHHHHCCEEEEEECCCCCCCCCCCCCHH
LFGRFVEMMERNR
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA