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The map label for this gene is tapB [H]

Identifier: 118580926

GI number: 118580926

Start: 2723222

End: 2724928

Strand: Reverse

Name: tapB [H]

Synonym: Ppro_2514

Alternate gene names: 118580926

Gene position: 2724928-2723222 (Counterclockwise)

Preceding gene: 118580927

Following gene: 118580925

Centisome position: 67.99

GC content: 59.64

Gene sequence:

>1707_bases
ATGCAGTTGAGCAGGCTGGGAGAAATCCTGGTAACCAACAACCTGATCACCAGAGAACAGCTTGCCAAGGCTCTCGAAGA
ACAGAAGATGTCCGGCGGGCAGGCCCGTCTCGGCACGATCCTGGTCAAGCAGAACCTGATCAGCGAAGAGAATCTGACGG
CATTTCTCTCCAAACAGTACGGTGTGCCCACGGTCAGCCTTTCCAACTATGACGTGGATCCGGCTGTTACCAAGATCATC
CAGTCCGAGGTTGTCCAGAAGTATCAACTGGTTCCCATCAACCGCGTCGGGGCGACCCTGGTCGTGGCGGTTACCGATCC
CTCCAATCTCTTCGCCATCGAAGACATCAAGTTCATGACCGGCTACAACGTGGAGATGGTCATCGCTTCGGAAAGCGATA
TCAAGGCGGCCATCGACAAGTACTACGACCAGTCCGCCTCCCTGGCCGATGTCATGGATGACATGAACCTGGAGGACCTG
GAGGTGGTTGATGTCGACGAGGATGTGGACATCAGCTCCATGGAGCGGGCCACGGAGGATGCGCCGGTGGTCAGGATGGT
CAATGCCATTCTGCAGGATGCCATCAAGAAGAAAGCCAGCGACATCCACATCGAGCCCTACGAGAAGCTGTTCCGGGTGC
GCTACCGCATCGACGGCGTGCTGTACGAGGCCATGAAGCCGCCGCTGAAGCTGAAGAACGCCATTACCTCGCGCATCAAG
ATCATGGCTGAGCTGGATATCGCCGAGCGCCGCCTCCCCCAGGACGGCCGCATCAAGATCAAGCTGGGCGGCGGAAAGGA
TATGGACTTCAGGGTCTCGGTCCTGCCGACTCTGTTCGGCGAGAAGATCTGCATGCGTCTATCGGACAAGAGCAGCCTGC
AGACCGATCTGACCAAGCTGGGCTACGAGCCGGAGGCCCTGGAGAACTTCCTGCGGGAGATTCACAAGCCCTTCGGCATG
GTGCTGGTCACCGGTCCCACCGGCAGTGGCAAGACCGTTTCGCTCTATTCGGCCATTGCCGAGCTGAACAAGGTCTCCGA
GAATATATCAACCGCCGAGGATCCGGTGGAGTTCAACTTTGCCGGCATCAACCAGGTCCAGATGCACGAGGACATCGGCC
TCAACTTCGCCGCCGCGTTGCGCTCCTTCCTGCGTCAGGATCCGGATATCATCATGATTGGCGAGATCCGCGACTTCGAA
ACCGCTGAGATCGCCGTCAAGGCCGCCCTGACCGGCCACCTGGTGCTCTCCACCCTGCATACCAACGATGCCCCCGCCAC
CATCAACCGCCTGCTGAACATGGGCATCGAGCCGTTTCTGGTGGCATCGGCGGTGAACCTGATCACCGCCCAGCGCCTGG
CACGGCGCGTCTGCAAGGAGTGCAAGGAGCCGGAGGATATTCCGGTCCAGGCGCTGATCGATGCCGGCGTGCCGCCCGAC
GAAGCCCCTGGCTATGTCTGCTACCGTGGCAAGGGGTGCCCGGTCTGTAACGGCACCGGCTACAAAGGGAGGGTTGGCTT
CTATCAGGTCATGCCGATGCTGGACGAGATCCGCGAACTGATCCTCAACGGGGCCAATACCGCCGAGATCAAGCGCGAAA
CCATGCGGCTGGGGATCAAGTCCATGCGCCAGTCCGGGCTCACCAAGCTCAAGGAAGGGGTTACCTCGTTCGAAGAGGTG
CTGCGGGTCACCATCGCCGATACCTAA

Upstream 100 bases:

>100_bases
TTGCTGTTTTTGCAACTATCTGTATTGTTTGACTATACTTGACATCCTCCGTCAATCCGCATACTATCTGTCATTACAAC
GTTATATTCCGGGAGTTTTT

Downstream 100 bases:

>100_bases
ATCCGTTACTGGTGACTGGTCACGAGTCACGAAAGGCGCATTCCATGCTCAGTCTTCACCAACTGCTCAAGACGCTGGTC
GAGGCCAACGGCTCCGACCT

Product: type IV-A pilus assembly ATPase PilB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 568; Mature: 568

Protein sequence:

>568_residues
MQLSRLGEILVTNNLITREQLAKALEEQKMSGGQARLGTILVKQNLISEENLTAFLSKQYGVPTVSLSNYDVDPAVTKII
QSEVVQKYQLVPINRVGATLVVAVTDPSNLFAIEDIKFMTGYNVEMVIASESDIKAAIDKYYDQSASLADVMDDMNLEDL
EVVDVDEDVDISSMERATEDAPVVRMVNAILQDAIKKKASDIHIEPYEKLFRVRYRIDGVLYEAMKPPLKLKNAITSRIK
IMAELDIAERRLPQDGRIKIKLGGGKDMDFRVSVLPTLFGEKICMRLSDKSSLQTDLTKLGYEPEALENFLREIHKPFGM
VLVTGPTGSGKTVSLYSAIAELNKVSENISTAEDPVEFNFAGINQVQMHEDIGLNFAAALRSFLRQDPDIIMIGEIRDFE
TAEIAVKAALTGHLVLSTLHTNDAPATINRLLNMGIEPFLVASAVNLITAQRLARRVCKECKEPEDIPVQALIDAGVPPD
EAPGYVCYRGKGCPVCNGTGYKGRVGFYQVMPMLDEIRELILNGANTAEIKRETMRLGIKSMRQSGLTKLKEGVTSFEEV
LRVTIADT

Sequences:

>Translated_568_residues
MQLSRLGEILVTNNLITREQLAKALEEQKMSGGQARLGTILVKQNLISEENLTAFLSKQYGVPTVSLSNYDVDPAVTKII
QSEVVQKYQLVPINRVGATLVVAVTDPSNLFAIEDIKFMTGYNVEMVIASESDIKAAIDKYYDQSASLADVMDDMNLEDL
EVVDVDEDVDISSMERATEDAPVVRMVNAILQDAIKKKASDIHIEPYEKLFRVRYRIDGVLYEAMKPPLKLKNAITSRIK
IMAELDIAERRLPQDGRIKIKLGGGKDMDFRVSVLPTLFGEKICMRLSDKSSLQTDLTKLGYEPEALENFLREIHKPFGM
VLVTGPTGSGKTVSLYSAIAELNKVSENISTAEDPVEFNFAGINQVQMHEDIGLNFAAALRSFLRQDPDIIMIGEIRDFE
TAEIAVKAALTGHLVLSTLHTNDAPATINRLLNMGIEPFLVASAVNLITAQRLARRVCKECKEPEDIPVQALIDAGVPPD
EAPGYVCYRGKGCPVCNGTGYKGRVGFYQVMPMLDEIRELILNGANTAEIKRETMRLGIKSMRQSGLTKLKEGVTSFEEV
LRVTIADT
>Mature_568_residues
MQLSRLGEILVTNNLITREQLAKALEEQKMSGGQARLGTILVKQNLISEENLTAFLSKQYGVPTVSLSNYDVDPAVTKII
QSEVVQKYQLVPINRVGATLVVAVTDPSNLFAIEDIKFMTGYNVEMVIASESDIKAAIDKYYDQSASLADVMDDMNLEDL
EVVDVDEDVDISSMERATEDAPVVRMVNAILQDAIKKKASDIHIEPYEKLFRVRYRIDGVLYEAMKPPLKLKNAITSRIK
IMAELDIAERRLPQDGRIKIKLGGGKDMDFRVSVLPTLFGEKICMRLSDKSSLQTDLTKLGYEPEALENFLREIHKPFGM
VLVTGPTGSGKTVSLYSAIAELNKVSENISTAEDPVEFNFAGINQVQMHEDIGLNFAAALRSFLRQDPDIIMIGEIRDFE
TAEIAVKAALTGHLVLSTLHTNDAPATINRLLNMGIEPFLVASAVNLITAQRLARRVCKECKEPEDIPVQALIDAGVPPD
EAPGYVCYRGKGCPVCNGTGYKGRVGFYQVMPMLDEIRELILNGANTAEIKRETMRLGIKSMRQSGLTKLKEGVTSFEEV
LRVTIADT

Specific function: Involved in the translocation of the type IV pilin [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=475, Percent_Identity=42.1052631578947, Blast_Score=367, Evalue=1e-102,
Organism=Escherichia coli, GI1786296, Length=382, Percent_Identity=43.717277486911, Blast_Score=336, Evalue=2e-93,
Organism=Escherichia coli, GI87082188, Length=247, Percent_Identity=33.1983805668016, Blast_Score=117, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 62808; Mature: 62808

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLSRLGEILVTNNLITREQLAKALEEQKMSGGQARLGTILVKQNLISEENLTAFLSKQY
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCC
GVPTVSLSNYDVDPAVTKIIQSEVVQKYQLVPINRVGATLVVAVTDPSNLFAIEDIKFMT
CCCEEEECCCCCCHHHHHHHHHHHHHHHCCCEEECCCCEEEEEEECCCCEEEEECEEEEC
GYNVEMVIASESDIKAAIDKYYDQSASLADVMDDMNLEDLEVVDVDEDVDISSMERATED
CCCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHCCC
APVVRMVNAILQDAIKKKASDIHIEPYEKLFRVRYRIDGVLYEAMKPPLKLKNAITSRIK
CHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
IMAELDIAERRLPQDGRIKIKLGGGKDMDFRVSVLPTLFGEKICMRLSDKSSLQTDLTKL
HHHHCCHHHHHCCCCCEEEEEECCCCCCCEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHC
GYEPEALENFLREIHKPFGMVLVTGPTGSGKTVSLYSAIAELNKVSENISTAEDPVEFNF
CCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEE
AGINQVQMHEDIGLNFAAALRSFLRQDPDIIMIGEIRDFETAEIAVKAALTGHLVLSTLH
CCCCCEEEHHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHEEEEC
TNDAPATINRLLNMGIEPFLVASAVNLITAQRLARRVCKECKEPEDIPVQALIDAGVPPD
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEECCCCCC
EAPGYVCYRGKGCPVCNGTGYKGRVGFYQVMPMLDEIRELILNGANTAEIKRETMRLGIK
CCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
SMRQSGLTKLKEGVTSFEEVLRVTIADT
HHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQLSRLGEILVTNNLITREQLAKALEEQKMSGGQARLGTILVKQNLISEENLTAFLSKQY
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCC
GVPTVSLSNYDVDPAVTKIIQSEVVQKYQLVPINRVGATLVVAVTDPSNLFAIEDIKFMT
CCCEEEECCCCCCHHHHHHHHHHHHHHHCCCEEECCCCEEEEEEECCCCEEEEECEEEEC
GYNVEMVIASESDIKAAIDKYYDQSASLADVMDDMNLEDLEVVDVDEDVDISSMERATED
CCCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHCCC
APVVRMVNAILQDAIKKKASDIHIEPYEKLFRVRYRIDGVLYEAMKPPLKLKNAITSRIK
CHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
IMAELDIAERRLPQDGRIKIKLGGGKDMDFRVSVLPTLFGEKICMRLSDKSSLQTDLTKL
HHHHCCHHHHHCCCCCEEEEEECCCCCCCEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHC
GYEPEALENFLREIHKPFGMVLVTGPTGSGKTVSLYSAIAELNKVSENISTAEDPVEFNF
CCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEE
AGINQVQMHEDIGLNFAAALRSFLRQDPDIIMIGEIRDFETAEIAVKAALTGHLVLSTLH
CCCCCEEEHHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHEEEEC
TNDAPATINRLLNMGIEPFLVASAVNLITAQRLARRVCKECKEPEDIPVQALIDAGVPPD
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEECCCCCC
EAPGYVCYRGKGCPVCNGTGYKGRVGFYQVMPMLDEIRELILNGANTAEIKRETMRLGIK
CCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
SMRQSGLTKLKEGVTSFEEVLRVTIADT
HHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8820654 [H]