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The map label for this gene is ksgA [H]

Identifier: 118580905

GI number: 118580905

Start: 2699765

End: 2700580

Strand: Reverse

Name: ksgA [H]

Synonym: Ppro_2492

Alternate gene names: 118580905

Gene position: 2700580-2699765 (Counterclockwise)

Preceding gene: 118580906

Following gene: 118580903

Centisome position: 67.38

GC content: 65.69

Gene sequence:

>816_bases
ATGAGCAGTGGACGGCGGCCCCTCAAGTCTCTGGGGCAGAACTTCCTGGTGGACCGGAATATTGTGGAGCGGATCATCCG
CGGCGCGGCCCTGGAGCCGGACGACCAGGTTCTGGAGGTGGGGCCGGGACGGGGCGCCCTGACCGCCCATCTGTCCGAAC
GCGTGGCCCGGCTGCTGCTGGTTGAGTATGACCATGCCCTGGCTGACGAGCTGTCCGAGCGCTACCGGGAGAATCAACGG
GTGCGGGTGATTGATGGCGATATCCTGGCGGTGGACCTGGAGGCGTTGCTGGAAGACGGTGACAAACGCTGGAAGGTGGT
CGCCAACCTCCCCTACAACATCTCCACCCAGGTGCTGTTCCGCTTCCTGGAGCTGCGCTCCCGGCTGTCGCGCATGCTGC
TCATGCTGCAGAAAGAGGTGGGGGACCGCCTGACCGCCCTGCCTGACTGCTCCGACTACGGGGTGACGACGGTGATCCTG
GGGCTCTGGTTCGACATCCGCCGCGAGTTCGTGGTCCCGCCCGGCTGTTTCCATCCCCGCCCCAGGGTCGACTCGGTGGT
GCTGAGCTTTACGCCCCTTGATGCCCCCCGCGCCCAGGTGGGCGACGAGGAGATCTTTCGAAGGGTGGTCAAGTGCGCCT
TCGCCACCCGCAGGAAGACGCTCTTCAACTGCCTCAAGCGGGGGGAACTGGTTGCTCCCGATGGCGTGGCCGAACTTCTG
GAGAGCTGCGGAATCGATGGCCGGCGCAGGGGGGAGACGCTCTCCCTGGAGGAGTTTGCCCGCCTCTCGCGCCATCTGTC
AGGCTACAGGGCATAA

Upstream 100 bases:

>100_bases
CGGTTCCGGGAAACCACTACCTGGAGCAGGGCCATGCCTCGGAGCTGAGCATGGATGTCACCGCCACCTGGGACATGGAC
CGGGTGGGGGAGGGGCGGTC

Downstream 100 bases:

>100_bases
AAAAACCGCCTTCGGGCGGTTTTTTTATGGTCGCTTGTTTTCGTGTGCGGAGGGTTACTCCTGGGGTGCCTGCTTTTTCG
CGGCCTCTGCTTTGGCTCCC

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MSSGRRPLKSLGQNFLVDRNIVERIIRGAALEPDDQVLEVGPGRGALTAHLSERVARLLLVEYDHALADELSERYRENQR
VRVIDGDILAVDLEALLEDGDKRWKVVANLPYNISTQVLFRFLELRSRLSRMLLMLQKEVGDRLTALPDCSDYGVTTVIL
GLWFDIRREFVVPPGCFHPRPRVDSVVLSFTPLDAPRAQVGDEEIFRRVVKCAFATRRKTLFNCLKRGELVAPDGVAELL
ESCGIDGRRRGETLSLEEFARLSRHLSGYRA

Sequences:

>Translated_271_residues
MSSGRRPLKSLGQNFLVDRNIVERIIRGAALEPDDQVLEVGPGRGALTAHLSERVARLLLVEYDHALADELSERYRENQR
VRVIDGDILAVDLEALLEDGDKRWKVVANLPYNISTQVLFRFLELRSRLSRMLLMLQKEVGDRLTALPDCSDYGVTTVIL
GLWFDIRREFVVPPGCFHPRPRVDSVVLSFTPLDAPRAQVGDEEIFRRVVKCAFATRRKTLFNCLKRGELVAPDGVAELL
ESCGIDGRRRGETLSLEEFARLSRHLSGYRA
>Mature_270_residues
SSGRRPLKSLGQNFLVDRNIVERIIRGAALEPDDQVLEVGPGRGALTAHLSERVARLLLVEYDHALADELSERYRENQRV
RVIDGDILAVDLEALLEDGDKRWKVVANLPYNISTQVLFRFLELRSRLSRMLLMLQKEVGDRLTALPDCSDYGVTTVILG
LWFDIRREFVVPPGCFHPRPRVDSVVLSFTPLDAPRAQVGDEEIFRRVVKCAFATRRKTLFNCLKRGELVAPDGVAELLE
SCGIDGRRRGETLSLEEFARLSRHLSGYRA

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI156415992, Length=277, Percent_Identity=30.6859205776173, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI7657198, Length=287, Percent_Identity=28.9198606271777, Blast_Score=98, Evalue=7e-21,
Organism=Escherichia coli, GI1786236, Length=259, Percent_Identity=32.046332046332, Blast_Score=144, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI25146882, Length=270, Percent_Identity=27.037037037037, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI25141369, Length=283, Percent_Identity=27.9151943462898, Blast_Score=87, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6324989, Length=215, Percent_Identity=29.3023255813954, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21357273, Length=281, Percent_Identity=30.9608540925267, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21358017, Length=221, Percent_Identity=29.4117647058824, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 30682; Mature: 30551

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSGRRPLKSLGQNFLVDRNIVERIIRGAALEPDDQVLEVGPGRGALTAHLSERVARLLL
CCCCCCHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHH
VEYDHALADELSERYRENQRVRVIDGDILAVDLEALLEDGDKRWKVVANLPYNISTQVLF
HHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHH
RFLELRSRLSRMLLMLQKEVGDRLTALPDCSDYGVTTVILGLWFDIRREFVVPPGCFHPR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
PRVDSVVLSFTPLDAPRAQVGDEEIFRRVVKCAFATRRKTLFNCLKRGELVAPDGVAELL
CCCCCEEEEECCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
ESCGIDGRRRGETLSLEEFARLSRHLSGYRA
HHCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SSGRRPLKSLGQNFLVDRNIVERIIRGAALEPDDQVLEVGPGRGALTAHLSERVARLLL
CCCCCHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHH
VEYDHALADELSERYRENQRVRVIDGDILAVDLEALLEDGDKRWKVVANLPYNISTQVLF
HHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHH
RFLELRSRLSRMLLMLQKEVGDRLTALPDCSDYGVTTVILGLWFDIRREFVVPPGCFHPR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
PRVDSVVLSFTPLDAPRAQVGDEEIFRRVVKCAFATRRKTLFNCLKRGELVAPDGVAELL
CCCCCEEEEECCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
ESCGIDGRRRGETLSLEEFARLSRHLSGYRA
HHCCCCCCCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA