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The map label for this gene is rlmL [H]

Identifier: 118580770

GI number: 118580770

Start: 2546560

End: 2547741

Strand: Reverse

Name: rlmL [H]

Synonym: Ppro_2355

Alternate gene names: 118580770

Gene position: 2547741-2546560 (Counterclockwise)

Preceding gene: 118580771

Following gene: 118580769

Centisome position: 63.57

GC content: 67.34

Gene sequence:

>1182_bases
ATGACCGAGAAGAAGCATATCCTCAGACGGGCGCCACGCCCGGAGCCGAAGCCGGACAGGGAGATGGAGTGCTTTGCCGC
CGTGCCGCGCGGCAGCGAGGACGTGACCGCGGCCGAACTGCGCCTGCTGGGCATCGAGGGGGCGCAGGCAGGGCGGGGCG
GGGTCTCGTTCCGCACCAACCGGGCCGGTCTGTACCGCGCCAATCTTTGGCTGCGCAGCGCCAGCCGGGTGCTGGTCAGG
CTGGCCGGGTTTCCCTGCTCCTCGCCGGCAGAGCTGTACGATGGCGTGCATGCCATCCCCTGGCAGGAGATGATCACACC
CGCCATGACCCTGGCGGTGGACTGCTCCCTGCGGGACTCGGCCCTGACCCATTCCGGTTTTGTGGCGCTGAAGACCAAGG
ATGCCATCGTGGACCGCATCCGTGAGGCATGCGGCAGTCGTCCCAACGTGGATACGGCCTCCCCGGACGTGCGGGTCAAT
ATCCACTTGGTGAAGAATGTCTGCACGGTCAGCCTGGACAGCTCGGGGGACGCCCTGGACCGTCGCGGCTACCGTCTGGA
GCGCAACGAGGCGCCCCTGCGCGAGACCCTGGCCGCCGCCATCATCGCGCTCACCGGCTGGGACGGCAACGTAGCCCTGG
CCGACCCCATGTGCGGCTCGGGCACCATCCCCATCGAGGCGGCGCTCATGGCCGCCCGCGTTCCGCCGGGGCGCAACCGC
TCATTCGGATTCCAGCGCTGGCAGGATTTCGACCCCCGCCTCTGGAAGGGGATCCAGGCCGAAGCGGAGAGCGGCATCCG
ACAGCTCCCGGTGGGGCTGGTCAGCGGCTACGACAGCGACAGCCGCGCCCTTTCCATTGCCCGGCGCAACGCGGCGGCGG
CAGGCTTCGAGGGGCAGGTGCACTTCTTCCACAGCTCCCTGGAGCAGTTCAGGCCGGAAGGGGAGGGTGGGGTGGTAATC
ATCAATCCTCCCTACGGCAAACGCATGGGGGAGGAGGATGAGCTGAAGGAGCTCTACTGCCAGATCGGCGACATCCTCAA
ACAGCGCTGCCAGGGGTGGACCGGCTATGTGCTTACCGGCAACCTGGAGCTGGCCAAGTACATCGGCCTGAAGGCGTCGC
GGCGGTTTGTGCTCTATAACGGCCCGATCGAGTGCCGCCTGCTCAAGTACGAGCTGTACTGA

Upstream 100 bases:

>100_bases
AGGAGCGCCATGGCCGCGTGATTATCGACAACAGCGGTACGCCCGAGGAGACGGAACGGCTGGTGGCGGACATATGGGAA
CGGGAAATGGGGGAACGAGC

Downstream 100 bases:

>100_bases
GCCTGAAACGGCGGTTCTCATCCACCGTCGAAGGATGTTTGTACACCATGCGGCAGTTGGGGGCTGCCTGCGTTCGCGCG
GACGGGCTCTTTCTGTACTT

Product: putative RNA methylase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MTEKKHILRRAPRPEPKPDREMECFAAVPRGSEDVTAAELRLLGIEGAQAGRGGVSFRTNRAGLYRANLWLRSASRVLVR
LAGFPCSSPAELYDGVHAIPWQEMITPAMTLAVDCSLRDSALTHSGFVALKTKDAIVDRIREACGSRPNVDTASPDVRVN
IHLVKNVCTVSLDSSGDALDRRGYRLERNEAPLRETLAAAIIALTGWDGNVALADPMCGSGTIPIEAALMAARVPPGRNR
SFGFQRWQDFDPRLWKGIQAEAESGIRQLPVGLVSGYDSDSRALSIARRNAAAAGFEGQVHFFHSSLEQFRPEGEGGVVI
INPPYGKRMGEEDELKELYCQIGDILKQRCQGWTGYVLTGNLELAKYIGLKASRRFVLYNGPIECRLLKYELY

Sequences:

>Translated_393_residues
MTEKKHILRRAPRPEPKPDREMECFAAVPRGSEDVTAAELRLLGIEGAQAGRGGVSFRTNRAGLYRANLWLRSASRVLVR
LAGFPCSSPAELYDGVHAIPWQEMITPAMTLAVDCSLRDSALTHSGFVALKTKDAIVDRIREACGSRPNVDTASPDVRVN
IHLVKNVCTVSLDSSGDALDRRGYRLERNEAPLRETLAAAIIALTGWDGNVALADPMCGSGTIPIEAALMAARVPPGRNR
SFGFQRWQDFDPRLWKGIQAEAESGIRQLPVGLVSGYDSDSRALSIARRNAAAAGFEGQVHFFHSSLEQFRPEGEGGVVI
INPPYGKRMGEEDELKELYCQIGDILKQRCQGWTGYVLTGNLELAKYIGLKASRRFVLYNGPIECRLLKYELY
>Mature_392_residues
TEKKHILRRAPRPEPKPDREMECFAAVPRGSEDVTAAELRLLGIEGAQAGRGGVSFRTNRAGLYRANLWLRSASRVLVRL
AGFPCSSPAELYDGVHAIPWQEMITPAMTLAVDCSLRDSALTHSGFVALKTKDAIVDRIREACGSRPNVDTASPDVRVNI
HLVKNVCTVSLDSSGDALDRRGYRLERNEAPLRETLAAAIIALTGWDGNVALADPMCGSGTIPIEAALMAARVPPGRNRS
FGFQRWQDFDPRLWKGIQAEAESGIRQLPVGLVSGYDSDSRALSIARRNAAAAGFEGQVHFFHSSLEQFRPEGEGGVVII
NPPYGKRMGEEDELKELYCQIGDILKQRCQGWTGYVLTGNLELAKYIGLKASRRFVLYNGPIECRLLKYELY

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=375, Percent_Identity=38.4, Blast_Score=241, Evalue=6e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 43271; Mature: 43140

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: PS51165 THUMP ; PS00092 N6_MTASE ; PS01261 UPF0020

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEKKHILRRAPRPEPKPDREMECFAAVPRGSEDVTAAELRLLGIEGAQAGRGGVSFRTN
CCCHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCEEEEEEEEECCCCCCCCCCCEEEEC
RAGLYRANLWLRSASRVLVRLAGFPCSSPAELYDGVHAIPWQEMITPAMTLAVDCSLRDS
CCCEEEEHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCHHHHHHHHEEEEEECCCCCC
ALTHSGFVALKTKDAIVDRIREACGSRPNVDTASPDVRVNIHLVKNVCTVSLDSSGDALD
CCCCCCEEEEECHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECEEEEEECCCCCHHH
RRGYRLERNEAPLRETLAAAIIALTGWDGNVALADPMCGSGTIPIEAALMAARVPPGRNR
HCCCEECCCCCHHHHHHHHHHEEEECCCCCEEEECCCCCCCCCCHHHHHHHHCCCCCCCC
SFGFQRWQDFDPRLWKGIQAEAESGIRQLPVGLVSGYDSDSRALSIARRNAAAAGFEGQV
CCCCHHHCCCCHHHHHCCHHHHHCCHHHCCHHHHCCCCCCHHHHHHHHHHHHCCCCCCEE
HFFHSSLEQFRPEGEGGVVIINPPYGKRMGEEDELKELYCQIGDILKQRCQGWTGYVLTG
HHHHHHHHHHCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
NLELAKYIGLKASRRFVLYNGPIECRLLKYELY
CCHHHHHHCCCCCCEEEEECCCEEEEEEEEECC
>Mature Secondary Structure 
TEKKHILRRAPRPEPKPDREMECFAAVPRGSEDVTAAELRLLGIEGAQAGRGGVSFRTN
CCHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCEEEEEEEEECCCCCCCCCCCEEEEC
RAGLYRANLWLRSASRVLVRLAGFPCSSPAELYDGVHAIPWQEMITPAMTLAVDCSLRDS
CCCEEEEHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCHHHHHHHHEEEEEECCCCCC
ALTHSGFVALKTKDAIVDRIREACGSRPNVDTASPDVRVNIHLVKNVCTVSLDSSGDALD
CCCCCCEEEEECHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECEEEEEECCCCCHHH
RRGYRLERNEAPLRETLAAAIIALTGWDGNVALADPMCGSGTIPIEAALMAARVPPGRNR
HCCCEECCCCCHHHHHHHHHHEEEECCCCCEEEECCCCCCCCCCHHHHHHHHCCCCCCCC
SFGFQRWQDFDPRLWKGIQAEAESGIRQLPVGLVSGYDSDSRALSIARRNAAAAGFEGQV
CCCCHHHCCCCHHHHHCCHHHHHCCHHHCCHHHHCCCCCCHHHHHHHHHHHHCCCCCCEE
HFFHSSLEQFRPEGEGGVVIINPPYGKRMGEEDELKELYCQIGDILKQRCQGWTGYVLTG
HHHHHHHHHHCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
NLELAKYIGLKASRRFVLYNGPIECRLLKYELY
CCHHHHHHCCCCCCEEEEECCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA