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The map label for this gene is ygfK [H]

Identifier: 118580575

GI number: 118580575

Start: 2327146

End: 2329461

Strand: Reverse

Name: ygfK [H]

Synonym: Ppro_2159

Alternate gene names: 118580575

Gene position: 2329461-2327146 (Counterclockwise)

Preceding gene: 118580576

Following gene: 118580574

Centisome position: 58.12

GC content: 64.03

Gene sequence:

>2316_bases
ATGGCAGCCTATATTTCAGGCTTTGATGAGCATAACAAACGCATATCCACGCAGCAGCTGCTGCAGAAGGTCTACGACGC
CCTGGATGCCGGGGAAACCGAATTCGAGATCCTGGCCTCTGGCCACCACGACATCGGCGGCCCGCTCTGGACAAAGGACG
GCACGCCGCTCAAGTTCCGGGTGAAAAACCCGGGGCAGCGGGTCGGCTCCTTCGGGCTGGAAGGGACCGAGATCATCATC
GACGGTCCGGCGCCGGCCGATGTGGGCTGGCTCAATGCCGGCGCCATCCTGACCCTCAAGGGGGACGGCGGCGACACAAC
CGCCCACTGCGCCGCCAGCGGCAAGATCTACGTGGGGGGGCGGGCCGGAACCCGCACCGGCTCCCTGATGAAGCACGACC
CGGCCTACGATGCCCCGGAGCTGTGGGTGCTGAAGAACACCGGCTCCTTCTCCTTCGAGTTCATGGGGGGCGGCATCGCC
GTGGTCTGCGGCTACGATTCCGAACAGTTCGAATCAATGCTGGGTGACCGCGGCTGCGTCGGCATGGTGGGGGGCACCAT
CTATGTGCGCGGGCCGGTCACGGGGCTGTCCAAATTCGTCTGGCAGCTGGAACTGGATGAGGCTGACCGGGAGTTCCTTT
CCGCCAACATGCCGGTCTTCCTGGAGAAGATCGAGCGGCCCCAGCTCTTGGCCGAACTGACCGACTTCTCCCAGTGGAAG
AAGATTACTCCCATGAGCTGGGAGGAGCGCCAGCGCCGCGAGCGGATCACCATGAGGGAGTTCCGCACCACCAAGTGGGT
GGAGGGGGGCATCTTCTCCGACGTGGTCTCGGATGACTACGACCGGGTGATCGGCTTCGTCAACACCGGCGAGGACCGGC
TCAAGATCCCCCACTGGCAGAACAAGCTCTACGGCGCTCCCTGCCAGACCGCCTGTCCCACCGGTATTCCCACCCAGGAC
CGCATCAACCTGCTGCGCCAGGGCAAGGTCAAGGAGGCGCTGGAGCTGGTGCTGACCTATTCTCCCTTCCCGGCCAGCGT
CTGCGGCCAGGTCTGTCCCAACCTGTGCAAGGATGCCTGCAGCCGCCAGTACATCGACCTTCCGGTGGCCATGCAGGAAC
TGGGCAGGCTCTCCCAGGCTGCTGCGCCACCGACCAGGAAGCCGAAAACCGGCAAGAGGGTGGCTGTCATCGGCGGCGGT
CCCGGTGGCCTCTCCGCTGCCTGGCAGCTCTCCCTTCTGGGGCACAGCGTGACTCTGTTCGAGGGAGACACGGAGGTGGG
GGGCAAGCTGCGCCAGGCCATCCCCATGGATCGACTGCCCCGGGAGATTCTGGAGAGCGAGGTCAACCGCATCAAGCAGA
TGGGAACCGACATCCGCGTTTCCCAGAAGATCGACAAGACCGCCTTTAGTGCCCTGCAGGATCAGTACGATGCCCTGGTG
CTGGCCAGCGGTGCCCACAACCCTGTGGTGATCCCCTTTCCCGGCCATGAGCGGCTGGTCAAGGGGCTGGATTTCCTGAA
GAGCATCAACAACGGTGAAAAACCCAGGGTCGGGAAAAAGGTTGTCGTCATCGGCGCCGGCAACGCCGGCATGGACGTGG
CTCTGGGGGCCTACGCCATGGGCGCCGAACGTGTCACCGCCATCGACGTGCAGCGCCCGGCAGCCTACCAGAAGGAGATC
GACCACTTCCACGCCCTGGGGGGAGAGATCCAGTGGCCGGTGTTCACCGAGCGCATCGATGACGACGGACTGCACACCAA
GGATGGCCGACTGATCGAGGCCGACACGGTGATCATCTCCATTGGCGAGCGGCCGGACCTCTCCTATGTGCCCCGAGAAT
GGCTCACCGATCGGGGGATGATGGATGTGAACGACTGCTGGCAGTCGCTCAGGAATGAAAAGGTCTTTGCCATCGGCGAT
ACCACCAAACCGGGACTCCTGACCCATGCCATCGCCGGCGGCCGCGAGGTGGCCGGATACATCGACGCCTACCTGAACGG
CTGGGAACTGGTTGCCGCCAAGAAGCCGGAGATGATCCCCCAGGAAAGGCTGAGCCGCGAGCTGTTCATGCCCCAGAATC
GCGGTCGTTTCCGTCTTAGGGATGCCAAGGACGAGGTGCACCGCTGCATCTCCTGCGGCACCTGCCGCGACTGCAGCATG
TGCCTGGAGACCTGTCCCGAGGGGGCCATCGTGCGCACCGAGAAGGAGGACGGCTCGGTGGAGTACACATCCGACGAGCG
CTACTGCATCGGCTGTGGCATCTGCGCCTCCATCTGCCCCTGCGGCGTCTGGGCCATGGAGAAGACCATTCTGTAG

Upstream 100 bases:

>100_bases
ACTCTGCGCTGCCGGTCGGCCGTTCCGACTGTCTGGTCTCAACCGATAAAGCCGTAGCGGACCGTCTGCAGGTCCAGTAC
GTCTGCTAAGGAGGATATAT

Downstream 100 bases:

>100_bases
CCTGACGGAGAAATAGTGAAACAGAGAGAGGCGGGACCATGTGGTATGGTTCCGCCTCTTATTTTTTGTATCTGACTCGT
CTCAATGCGTCTGCAAGCTG

Product: glutamate synthase alpha subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 771; Mature: 770

Protein sequence:

>771_residues
MAAYISGFDEHNKRISTQQLLQKVYDALDAGETEFEILASGHHDIGGPLWTKDGTPLKFRVKNPGQRVGSFGLEGTEIII
DGPAPADVGWLNAGAILTLKGDGGDTTAHCAASGKIYVGGRAGTRTGSLMKHDPAYDAPELWVLKNTGSFSFEFMGGGIA
VVCGYDSEQFESMLGDRGCVGMVGGTIYVRGPVTGLSKFVWQLELDEADREFLSANMPVFLEKIERPQLLAELTDFSQWK
KITPMSWEERQRRERITMREFRTTKWVEGGIFSDVVSDDYDRVIGFVNTGEDRLKIPHWQNKLYGAPCQTACPTGIPTQD
RINLLRQGKVKEALELVLTYSPFPASVCGQVCPNLCKDACSRQYIDLPVAMQELGRLSQAAAPPTRKPKTGKRVAVIGGG
PGGLSAAWQLSLLGHSVTLFEGDTEVGGKLRQAIPMDRLPREILESEVNRIKQMGTDIRVSQKIDKTAFSALQDQYDALV
LASGAHNPVVIPFPGHERLVKGLDFLKSINNGEKPRVGKKVVVIGAGNAGMDVALGAYAMGAERVTAIDVQRPAAYQKEI
DHFHALGGEIQWPVFTERIDDDGLHTKDGRLIEADTVIISIGERPDLSYVPREWLTDRGMMDVNDCWQSLRNEKVFAIGD
TTKPGLLTHAIAGGREVAGYIDAYLNGWELVAAKKPEMIPQERLSRELFMPQNRGRFRLRDAKDEVHRCISCGTCRDCSM
CLETCPEGAIVRTEKEDGSVEYTSDERYCIGCGICASICPCGVWAMEKTIL

Sequences:

>Translated_771_residues
MAAYISGFDEHNKRISTQQLLQKVYDALDAGETEFEILASGHHDIGGPLWTKDGTPLKFRVKNPGQRVGSFGLEGTEIII
DGPAPADVGWLNAGAILTLKGDGGDTTAHCAASGKIYVGGRAGTRTGSLMKHDPAYDAPELWVLKNTGSFSFEFMGGGIA
VVCGYDSEQFESMLGDRGCVGMVGGTIYVRGPVTGLSKFVWQLELDEADREFLSANMPVFLEKIERPQLLAELTDFSQWK
KITPMSWEERQRRERITMREFRTTKWVEGGIFSDVVSDDYDRVIGFVNTGEDRLKIPHWQNKLYGAPCQTACPTGIPTQD
RINLLRQGKVKEALELVLTYSPFPASVCGQVCPNLCKDACSRQYIDLPVAMQELGRLSQAAAPPTRKPKTGKRVAVIGGG
PGGLSAAWQLSLLGHSVTLFEGDTEVGGKLRQAIPMDRLPREILESEVNRIKQMGTDIRVSQKIDKTAFSALQDQYDALV
LASGAHNPVVIPFPGHERLVKGLDFLKSINNGEKPRVGKKVVVIGAGNAGMDVALGAYAMGAERVTAIDVQRPAAYQKEI
DHFHALGGEIQWPVFTERIDDDGLHTKDGRLIEADTVIISIGERPDLSYVPREWLTDRGMMDVNDCWQSLRNEKVFAIGD
TTKPGLLTHAIAGGREVAGYIDAYLNGWELVAAKKPEMIPQERLSRELFMPQNRGRFRLRDAKDEVHRCISCGTCRDCSM
CLETCPEGAIVRTEKEDGSVEYTSDERYCIGCGICASICPCGVWAMEKTIL
>Mature_770_residues
AAYISGFDEHNKRISTQQLLQKVYDALDAGETEFEILASGHHDIGGPLWTKDGTPLKFRVKNPGQRVGSFGLEGTEIIID
GPAPADVGWLNAGAILTLKGDGGDTTAHCAASGKIYVGGRAGTRTGSLMKHDPAYDAPELWVLKNTGSFSFEFMGGGIAV
VCGYDSEQFESMLGDRGCVGMVGGTIYVRGPVTGLSKFVWQLELDEADREFLSANMPVFLEKIERPQLLAELTDFSQWKK
ITPMSWEERQRRERITMREFRTTKWVEGGIFSDVVSDDYDRVIGFVNTGEDRLKIPHWQNKLYGAPCQTACPTGIPTQDR
INLLRQGKVKEALELVLTYSPFPASVCGQVCPNLCKDACSRQYIDLPVAMQELGRLSQAAAPPTRKPKTGKRVAVIGGGP
GGLSAAWQLSLLGHSVTLFEGDTEVGGKLRQAIPMDRLPREILESEVNRIKQMGTDIRVSQKIDKTAFSALQDQYDALVL
ASGAHNPVVIPFPGHERLVKGLDFLKSINNGEKPRVGKKVVVIGAGNAGMDVALGAYAMGAERVTAIDVQRPAAYQKEID
HFHALGGEIQWPVFTERIDDDGLHTKDGRLIEADTVIISIGERPDLSYVPREWLTDRGMMDVNDCWQSLRNEKVFAIGDT
TKPGLLTHAIAGGREVAGYIDAYLNGWELVAAKKPEMIPQERLSRELFMPQNRGRFRLRDAKDEVHRCISCGTCRDCSMC
LETCPEGAIVRTEKEDGSVEYTSDERYCIGCGICASICPCGVWAMEKTIL

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=286, Percent_Identity=26.5734265734266, Blast_Score=94, Evalue=5e-19,
Organism=Escherichia coli, GI1789243, Length=511, Percent_Identity=27.9843444227006, Blast_Score=144, Evalue=3e-35,
Organism=Escherichia coli, GI1788468, Length=383, Percent_Identity=28.1984334203655, Blast_Score=135, Evalue=7e-33,
Organism=Escherichia coli, GI87082180, Length=336, Percent_Identity=30.6547619047619, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1789606, Length=421, Percent_Identity=25.8907363420428, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI1788811, Length=416, Percent_Identity=26.6826923076923, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1789463, Length=148, Percent_Identity=33.1081081081081, Blast_Score=67, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17570289, Length=546, Percent_Identity=24.3589743589744, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71984108, Length=285, Percent_Identity=26.3157894736842, Blast_Score=86, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6320030, Length=434, Percent_Identity=26.2672811059908, Blast_Score=103, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24665547, Length=460, Percent_Identity=26.9565217391304, Blast_Score=127, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24665543, Length=460, Percent_Identity=26.9565217391304, Blast_Score=127, Evalue=3e-29,
Organism=Drosophila melanogaster, GI28574881, Length=460, Percent_Identity=26.9565217391304, Blast_Score=126, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24665539, Length=460, Percent_Identity=26.9565217391304, Blast_Score=126, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24640763, Length=280, Percent_Identity=26.4285714285714, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI18858217, Length=280, Percent_Identity=26.4285714285714, Blast_Score=92, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR017701 [H]

Pfam domain/function: PF07992 Pyr_redox_2 [H]

EC number: NA

Molecular weight: Translated: 84699; Mature: 84567

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAYISGFDEHNKRISTQQLLQKVYDALDAGETEFEILASGHHDIGGPLWTKDGTPLKFR
CCCCCCCCHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEECCCCCCEEEE
VKNPGQRVGSFGLEGTEIIIDGPAPADVGWLNAGAILTLKGDGGDTTAHCAASGKIYVGG
ECCCHHHHHCCCCCCCEEEECCCCCCCCCEECCCEEEEEECCCCCCCEEEECCCEEEECC
RAGTRTGSLMKHDPAYDAPELWVLKNTGSFSFEFMGGGIAVVCGYDSEQFESMLGDRGCV
CCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCEEEEECCCHHHHHHHHCCCCCE
GMVGGTIYVRGPVTGLSKFVWQLELDEADREFLSANMPVFLEKIERPQLLAELTDFSQWK
EEECCEEEEECCHHHHHHHHEEEECCHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHC
KITPMSWEERQRRERITMREFRTTKWVEGGIFSDVVSDDYDRVIGFVNTGEDRLKIPHWQ
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHEEEEECCCCCEECCCHH
NKLYGAPCQTACPTGIPTQDRINLLRQGKVKEALELVLTYSPFPASVCGQVCPNLCKDAC
CCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
SRQYIDLPVAMQELGRLSQAAAPPTRKPKTGKRVAVIGGGPGGLSAAWQLSLLGHSVTLF
CCEEECCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCHHEEEEEECCEEEEE
EGDTEVGGKLRQAIPMDRLPREILESEVNRIKQMGTDIRVSQKIDKTAFSALQDQYDALV
ECCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCEEE
LASGAHNPVVIPFPGHERLVKGLDFLKSINNGEKPRVGKKVVVIGAGNAGMDVALGAYAM
EECCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHH
GAERVTAIDVQRPAAYQKEIDHFHALGGEIQWPVFTERIDDDGLHTKDGRLIEADTVIIS
CCCCEEEEECCCCHHHHHHHHHHHHCCCCEECCHHHCCCCCCCCCCCCCEEEEEEEEEEE
IGERPDLSYVPREWLTDRGMMDVNDCWQSLRNEKVFAIGDTTKPGLLTHAIAGGREVAGY
ECCCCCCCCCCHHHHCCCCCCCHHHHHHHHCCCEEEEECCCCCCCHHHHHHCCCHHHHHH
IDAYLNGWELVAAKKPEMIPQERLSRELFMPQNRGRFRLRDAKDEVHRCISCGTCRDCSM
HHHHCCCEEEEECCCCCCCCHHHHHHHHCCCCCCCCEEEECCHHHHHHHHCCCCCCHHHH
CLETCPEGAIVRTEKEDGSVEYTSDERYCIGCGICASICPCGVWAMEKTIL
HHHHCCCCCEEEEECCCCCEEEECCCCEEEECCHHHHHHCCCHHHHHHHCC
>Mature Secondary Structure 
AAYISGFDEHNKRISTQQLLQKVYDALDAGETEFEILASGHHDIGGPLWTKDGTPLKFR
CCCCCCCHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEECCCCCCEEEE
VKNPGQRVGSFGLEGTEIIIDGPAPADVGWLNAGAILTLKGDGGDTTAHCAASGKIYVGG
ECCCHHHHHCCCCCCCEEEECCCCCCCCCEECCCEEEEEECCCCCCCEEEECCCEEEECC
RAGTRTGSLMKHDPAYDAPELWVLKNTGSFSFEFMGGGIAVVCGYDSEQFESMLGDRGCV
CCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCEEEEECCCHHHHHHHHCCCCCE
GMVGGTIYVRGPVTGLSKFVWQLELDEADREFLSANMPVFLEKIERPQLLAELTDFSQWK
EEECCEEEEECCHHHHHHHHEEEECCHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHC
KITPMSWEERQRRERITMREFRTTKWVEGGIFSDVVSDDYDRVIGFVNTGEDRLKIPHWQ
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHEEEEECCCCCEECCCHH
NKLYGAPCQTACPTGIPTQDRINLLRQGKVKEALELVLTYSPFPASVCGQVCPNLCKDAC
CCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
SRQYIDLPVAMQELGRLSQAAAPPTRKPKTGKRVAVIGGGPGGLSAAWQLSLLGHSVTLF
CCEEECCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCHHEEEEEECCEEEEE
EGDTEVGGKLRQAIPMDRLPREILESEVNRIKQMGTDIRVSQKIDKTAFSALQDQYDALV
ECCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCEEE
LASGAHNPVVIPFPGHERLVKGLDFLKSINNGEKPRVGKKVVVIGAGNAGMDVALGAYAM
EECCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHH
GAERVTAIDVQRPAAYQKEIDHFHALGGEIQWPVFTERIDDDGLHTKDGRLIEADTVIIS
CCCCEEEEECCCCHHHHHHHHHHHHCCCCEECCHHHCCCCCCCCCCCCCEEEEEEEEEEE
IGERPDLSYVPREWLTDRGMMDVNDCWQSLRNEKVFAIGDTTKPGLLTHAIAGGREVAGY
ECCCCCCCCCCHHHHCCCCCCCHHHHHHHHCCCEEEEECCCCCCCHHHHHHCCCHHHHHH
IDAYLNGWELVAAKKPEMIPQERLSRELFMPQNRGRFRLRDAKDEVHRCISCGTCRDCSM
HHHHCCCEEEEECCCCCCCCHHHHHHHHCCCCCCCCEEEECCHHHHHHHHCCCCCCHHHH
CLETCPEGAIVRTEKEDGSVEYTSDERYCIGCGICASICPCGVWAMEKTIL
HHHHCCCCCEEEEECCCCCEEEECCCCEEEECCHHHHHHCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Iron-Sulfur [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]