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The map label for this gene is dut [H]

Identifier: 118580532

GI number: 118580532

Start: 2275845

End: 2276309

Strand: Direct

Name: dut [H]

Synonym: Ppro_2116

Alternate gene names: 118580532

Gene position: 2275845-2276309 (Clockwise)

Preceding gene: 118580515

Following gene: 118580534

Centisome position: 56.78

GC content: 60.65

Gene sequence:

>465_bases
ATGAACAGACACAAAATTGAAACCAAAATCATGAATCCCCTGATGGGCTCGAAGATACCGCTCCCCTGCTACGCCACCGA
TGGCGCGGCTGCCATGGACCTGCGCGCCTGCCTGGAGGGGCCGCGTACCGTTCAACCGGGAGAAACAGCCCTAATCCCCA
GCGGCATAGCCATCGCCATCCACGACCCCGGCCTGGTGGCGCTGATCGTGCCCCGTTCAGGCCTGGGGATCAAACATGGC
ATCGTGCTGGCAAATACGGTGGGCGTCATCGATTCCGACTACCAGGGGGAGATAGGGATCGGCATCCACAACCGGGGAGA
GGCGCCCTACACCATCGAACCGGGTGAGCGCATCTGCCAGATGATGTTCATGCCGGTGGTCCAGGTCACCCTGAACCTGG
TCGAGTCGTTCAGCAGGGAGAGTGAACGGGGAGCAGGGGGATTCGGCCATACGGGAAAAATCTGA

Upstream 100 bases:

>100_bases
CCGAATGGTTGTGCTGTTTCAGCGCAAGATTCCGGTTTCCGGACAGGCACTTCTCTGCTACCATGGCGGTCCAATCCAAC
CTGTAAGCATTGGAATTTAC

Downstream 100 bases:

>100_bases
GGGGATAGCCACAGGGGGATGCTGTCGTGAAAGGTGCGCCAGGCGCCGGGATCAGAGCCGCAGCAGATCCTCCGTCAGCC
CGGTATGCCACGTTTTCCCC

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase Dut

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase [H]

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MNRHKIETKIMNPLMGSKIPLPCYATDGAAAMDLRACLEGPRTVQPGETALIPSGIAIAIHDPGLVALIVPRSGLGIKHG
IVLANTVGVIDSDYQGEIGIGIHNRGEAPYTIEPGERICQMMFMPVVQVTLNLVESFSRESERGAGGFGHTGKI

Sequences:

>Translated_154_residues
MNRHKIETKIMNPLMGSKIPLPCYATDGAAAMDLRACLEGPRTVQPGETALIPSGIAIAIHDPGLVALIVPRSGLGIKHG
IVLANTVGVIDSDYQGEIGIGIHNRGEAPYTIEPGERICQMMFMPVVQVTLNLVESFSRESERGAGGFGHTGKI
>Mature_154_residues
MNRHKIETKIMNPLMGSKIPLPCYATDGAAAMDLRACLEGPRTVQPGETALIPSGIAIAIHDPGLVALIVPRSGLGIKHG
IVLANTVGVIDSDYQGEIGIGIHNRGEAPYTIEPGERICQMMFMPVVQVTLNLVESFSRESERGAGGFGHTGKI

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA [H]

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family [H]

Homologues:

Organism=Homo sapiens, GI70906444, Length=132, Percent_Identity=34.8484848484849, Blast_Score=76, Evalue=1e-14,
Organism=Homo sapiens, GI4503423, Length=132, Percent_Identity=34.8484848484849, Blast_Score=76, Evalue=1e-14,
Organism=Homo sapiens, GI70906441, Length=132, Percent_Identity=34.8484848484849, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1790071, Length=149, Percent_Identity=57.7181208053691, Blast_Score=187, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI71988561, Length=134, Percent_Identity=38.0597014925373, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6319729, Length=89, Percent_Identity=39.3258426966292, Blast_Score=69, Evalue=4e-13,
Organism=Drosophila melanogaster, GI19921126, Length=153, Percent_Identity=28.1045751633987, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24583610, Length=150, Percent_Identity=28.6666666666667, Blast_Score=63, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008180
- InterPro:   IPR008181 [H]

Pfam domain/function: PF00692 dUTPase [H]

EC number: =3.6.1.23 [H]

Molecular weight: Translated: 16343; Mature: 16343

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRHKIETKIMNPLMGSKIPLPCYATDGAAAMDLRACLEGPRTVQPGETALIPSGIAIAI
CCCCHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHCCCCCCCCCCEEECCCCEEEEE
HDPGLVALIVPRSGLGIKHGIVLANTVGVIDSDYQGEIGIGIHNRGEAPYTIEPGERICQ
ECCCEEEEEECCCCCCCCCCEEEEEEECEECCCCCCEEEEEECCCCCCCEEECCHHHHHH
MMFMPVVQVTLNLVESFSRESERGAGGFGHTGKI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MNRHKIETKIMNPLMGSKIPLPCYATDGAAAMDLRACLEGPRTVQPGETALIPSGIAIAI
CCCCHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHCCCCCCCCCCEEECCCCEEEEE
HDPGLVALIVPRSGLGIKHGIVLANTVGVIDSDYQGEIGIGIHNRGEAPYTIEPGERICQ
ECCCEEEEEECCCCCCCCCCEEEEEEECEECCCCCCEEEEEECCCCCCCEEECCHHHHHH
MMFMPVVQVTLNLVESFSRESERGAGGFGHTGKI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA