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The map label for this gene is yciL [C]

Identifier: 118580486

GI number: 118580486

Start: 2223162

End: 2223977

Strand: Direct

Name: yciL [C]

Synonym: Ppro_2070

Alternate gene names: 118580486

Gene position: 2223162-2223977 (Clockwise)

Preceding gene: 118580485

Following gene: 118580489

Centisome position: 55.47

GC content: 65.69

Gene sequence:

>816_bases
ATGCAGGAACGCTTACAGAAACTCATTTCCCAGGCCGGCATCGCCTCGCGCCGGGCAGCGGAAGAGATGATCCTGGCCGG
CCGGGTAACGGTGAACGGCCTGGTTGTCACCGAACTGGGCAGCAAGGCCGACCCGACCGTGGACAGCATAGCCGTGGACG
GCAGGCCGGTCAAACCGGCAAGCCAGCACCTGTACATCCTGCTCAACAAGCCGGCCGGCTTCATGACCACCCTGGACGAT
CCTGAGGGACGCCAGGTGGTGACCGACCTGCTGCCGGAGATCAAGGAGCGGGTCTACCCGGTGGGGCGGCTGGATTACAA
CACCGAAGGGCTGCTGCTTTTGACCAACGACGGCGACTGGGCCAACAAACTGATGCACCCGCGCCACGAGATCGAAAAGG
AGTACCACGTCAGAGTGCGGGGCAAGGTGCACCAGAGTCAGATCGACCAGTTGAAGAACGGCGTTGATCTGGACGGCAGG
CCCACAGCACCGGCCCAGGTGCGGCTGATCAAGGATGGCGAGCAGAACGACTGGCTCTCCATCACCATCCACGAGGGGCG
CAACCGCCAGGTTCGGCGCATGTGCGAGGCGGTCAGCCTGTCGGTGGTGCGGTTGAAGCGGGTACGCTACGGTGCCCTGG
AGATGCGCGGCCTCAAGCCGGGCCAGCACCGGCTGCTGACCGAGGCCGAGGCCAGGGCGCTGCTGACTCCGGGAGCAGGG
TTACGGTCGGCGGAGGCGCCGAGGGAGAAGAGAGCCAGAAACGCCGGCCAGCCCCCCCGGGAGACGCGGGGACGCGGAAA
GAAGCCGACAACCTGA

Upstream 100 bases:

>100_bases
CATTGCGGCTGGTGATTGTCACCGGTCTTCAGGCTTTTGGTTTATCCGCGTTAATCCGTTTTTTTCGATTTTTCCGCGTT
CCATTTTATAAGGAGCGGCA

Downstream 100 bases:

>100_bases
ATTACGGTTGCACAAAAAACGCCAGGGGTTTCCCTGGCGTTTTTTATCACGGTGCACGTCAATTCCAGCCAAATAAGGCT
CTATTAAAAGTCGATCCGCC

Product: pseudouridine synthase

Products: NA

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MQERLQKLISQAGIASRRAAEEMILAGRVTVNGLVVTELGSKADPTVDSIAVDGRPVKPASQHLYILLNKPAGFMTTLDD
PEGRQVVTDLLPEIKERVYPVGRLDYNTEGLLLLTNDGDWANKLMHPRHEIEKEYHVRVRGKVHQSQIDQLKNGVDLDGR
PTAPAQVRLIKDGEQNDWLSITIHEGRNRQVRRMCEAVSLSVVRLKRVRYGALEMRGLKPGQHRLLTEAEARALLTPGAG
LRSAEAPREKRARNAGQPPRETRGRGKKPTT

Sequences:

>Translated_271_residues
MQERLQKLISQAGIASRRAAEEMILAGRVTVNGLVVTELGSKADPTVDSIAVDGRPVKPASQHLYILLNKPAGFMTTLDD
PEGRQVVTDLLPEIKERVYPVGRLDYNTEGLLLLTNDGDWANKLMHPRHEIEKEYHVRVRGKVHQSQIDQLKNGVDLDGR
PTAPAQVRLIKDGEQNDWLSITIHEGRNRQVRRMCEAVSLSVVRLKRVRYGALEMRGLKPGQHRLLTEAEARALLTPGAG
LRSAEAPREKRARNAGQPPRETRGRGKKPTT
>Mature_271_residues
MQERLQKLISQAGIASRRAAEEMILAGRVTVNGLVVTELGSKADPTVDSIAVDGRPVKPASQHLYILLNKPAGFMTTLDD
PEGRQVVTDLLPEIKERVYPVGRLDYNTEGLLLLTNDGDWANKLMHPRHEIEKEYHVRVRGKVHQSQIDQLKNGVDLDGR
PTAPAQVRLIKDGEQNDWLSITIHEGRNRQVRRMCEAVSLSVVRLKRVRYGALEMRGLKPGQHRLLTEAEARALLTPGAG
LRSAEAPREKRARNAGQPPRETRGRGKKPTT

Specific function: Unknown

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=241, Percent_Identity=42.7385892116183, Blast_Score=166, Evalue=1e-42,
Organism=Escherichia coli, GI1788510, Length=237, Percent_Identity=37.5527426160338, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI1790453, Length=234, Percent_Identity=37.1794871794872, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI87081838, Length=178, Percent_Identity=35.9550561797753, Blast_Score=106, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 30237; Mature: 30237

Theoretical pI: Translated: 10.51; Mature: 10.51

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQERLQKLISQAGIASRRAAEEMILAGRVTVNGLVVTELGSKADPTVDSIAVDGRPVKPA
CHHHHHHHHHHCCCHHHHHHHHHHHHCEEEECEEEEEECCCCCCCCHHEEEECCCCCCCC
SQHLYILLNKPAGFMTTLDDPEGRQVVTDLLPEIKERVYPVGRLDYNTEGLLLLTNDGDW
CCEEEEEEECCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCH
ANKLMHPRHEIEKEYHVRVRGKVHQSQIDQLKNGVDLDGRPTAPAQVRLIKDGEQNDWLS
HHHHCCCHHHHCCEEEEEEECCHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCEEE
ITIHEGRNRQVRRMCEAVSLSVVRLKRVRYGALEMRGLKPGQHRLLTEAEARALLTPGAG
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHCHHEECCCCCCHHHHHHHHHHHEEECCCCC
LRSAEAPREKRARNAGQPPRETRGRGKKPTT
CCCCCCCHHHHHHCCCCCCHHHCCCCCCCCC
>Mature Secondary Structure
MQERLQKLISQAGIASRRAAEEMILAGRVTVNGLVVTELGSKADPTVDSIAVDGRPVKPA
CHHHHHHHHHHCCCHHHHHHHHHHHHCEEEECEEEEEECCCCCCCCHHEEEECCCCCCCC
SQHLYILLNKPAGFMTTLDDPEGRQVVTDLLPEIKERVYPVGRLDYNTEGLLLLTNDGDW
CCEEEEEEECCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCH
ANKLMHPRHEIEKEYHVRVRGKVHQSQIDQLKNGVDLDGRPTAPAQVRLIKDGEQNDWLS
HHHHCCCHHHHCCEEEEEEECCHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCEEE
ITIHEGRNRQVRRMCEAVSLSVVRLKRVRYGALEMRGLKPGQHRLLTEAEARALLTPGAG
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHCHHEECCCCCCHHHHHHHHHHHEEECCCCC
LRSAEAPREKRARNAGQPPRETRGRGKKPTT
CCCCCCCHHHHHHCCCCCCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]