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The map label for this gene is pleD [H]

Identifier: 118580478

GI number: 118580478

Start: 2211478

End: 2212131

Strand: Direct

Name: pleD [H]

Synonym: Ppro_2062

Alternate gene names: 118580478

Gene position: 2211478-2212131 (Clockwise)

Preceding gene: 118580477

Following gene: 118580479

Centisome position: 55.18

GC content: 58.72

Gene sequence:

>654_bases
ATGGAGAGACAGAGAATCCTGATCGTCGATGACACCCCGGAGAACCTCCAGTTGCTCTACCGCACTCTCAGGGACGAGTA
TGACCTCTACGGAGCCACCAATGGCAGGGAAGCGCTCATGATCGCAGCGGCGCAGCAGCCCGACCTGATACTCCTCGATA
TCATGATGCCAGAGATGGATGGCTATGAAGTCTGCCGCCGACTGAAGAGCAGCGAAGAGCTCAGGGATGTGCCGGTACTG
TTCCTGACGGCCCTGTCCGAGGACAGCGATGAACTGCGCGGCCTGGAGATCGGGGCGGTGGATTACATCACCAAACCGTT
CAAGACCGCCGTCGTCAAACGGAGAATCGACACCCACCTCGCCCTGAAGCGCAAGACGGACCAGCTGGCCCGGAGCAATG
CGGAATTGCGCGAGGCCCTTGAAAACGTAAGGGCACTGTCCGGCCTGCTGCCGATCTGTTCGAGATGCAAGAAAATCCGC
GACGACCAGGGGTACTGGAAACATGTGGAAACCTATATCAGCCTGCATTCCGATGTGCTCTTCAGCCACGGCTACTGCCC
GGAATGCGCCGAAAAGGCCATGGATGACCTGAAGGTCCAGATGGAGCGCTACCCTCCCCGATCCCCGTCCACGGATGAGA
CAGACAGGTGGTAA

Upstream 100 bases:

>100_bases
TTCCGGGACCATCTCAAACGCTTCAATTTTGACGGGGCATTGGGCTGCCTGGACGAGCTGTACCGCGAGTTCGATATTAC
ACGTGAGACGGGAAACTTCC

Downstream 100 bases:

>100_bases
CACCCTGTCCGTGTCCCACCCCGCACGTTCGACTGCTGTTCACGGCTAGAGGCCGTTGATACAGACAGTTCCCGTTTTTT
TCTCACCGCTGTCCCCGATC

Product: response regulator receiver protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MERQRILIVDDTPENLQLLYRTLRDEYDLYGATNGREALMIAAAQQPDLILLDIMMPEMDGYEVCRRLKSSEELRDVPVL
FLTALSEDSDELRGLEIGAVDYITKPFKTAVVKRRIDTHLALKRKTDQLARSNAELREALENVRALSGLLPICSRCKKIR
DDQGYWKHVETYISLHSDVLFSHGYCPECAEKAMDDLKVQMERYPPRSPSTDETDRW

Sequences:

>Translated_217_residues
MERQRILIVDDTPENLQLLYRTLRDEYDLYGATNGREALMIAAAQQPDLILLDIMMPEMDGYEVCRRLKSSEELRDVPVL
FLTALSEDSDELRGLEIGAVDYITKPFKTAVVKRRIDTHLALKRKTDQLARSNAELREALENVRALSGLLPICSRCKKIR
DDQGYWKHVETYISLHSDVLFSHGYCPECAEKAMDDLKVQMERYPPRSPSTDETDRW
>Mature_217_residues
MERQRILIVDDTPENLQLLYRTLRDEYDLYGATNGREALMIAAAQQPDLILLDIMMPEMDGYEVCRRLKSSEELRDVPVL
FLTALSEDSDELRGLEIGAVDYITKPFKTAVVKRRIDTHLALKRKTDQLARSNAELREALENVRALSGLLPICSRCKKIR
DDQGYWKHVETYISLHSDVLFSHGYCPECAEKAMDDLKVQMERYPPRSPSTDETDRW

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1788394, Length=132, Percent_Identity=40.1515151515151, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1790860, Length=121, Percent_Identity=38.8429752066116, Blast_Score=74, Evalue=5e-15,
Organism=Escherichia coli, GI1788550, Length=105, Percent_Identity=39.0476190476191, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI1786784, Length=119, Percent_Identity=36.9747899159664, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786599, Length=128, Percent_Identity=36.71875, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI87082012, Length=148, Percent_Identity=31.7567567567568, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI1789809, Length=129, Percent_Identity=34.8837209302326, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI1786911, Length=130, Percent_Identity=36.1538461538462, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1790863, Length=111, Percent_Identity=36.9369369369369, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI1788713, Length=143, Percent_Identity=36.3636363636364, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1790437, Length=103, Percent_Identity=36.8932038834951, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 25080; Mature: 25080

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERQRILIVDDTPENLQLLYRTLRDEYDLYGATNGREALMIAAAQQPDLILLDIMMPEMD
CCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCEEEEEEECCCCC
GYEVCRRLKSSEELRDVPVLFLTALSEDSDELRGLEIGAVDYITKPFKTAVVKRRIDTHL
HHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHCCCEECHHHHHHHHHHHHHHHHHHHHHH
ALKRKTDQLARSNAELREALENVRALSGLLPICSRCKKIRDDQGYWKHVETYISLHSDVL
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
FSHGYCPECAEKAMDDLKVQMERYPPRSPSTDETDRW
HCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MERQRILIVDDTPENLQLLYRTLRDEYDLYGATNGREALMIAAAQQPDLILLDIMMPEMD
CCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCEEEEEEECCCCC
GYEVCRRLKSSEELRDVPVLFLTALSEDSDELRGLEIGAVDYITKPFKTAVVKRRIDTHL
HHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHCCCEECHHHHHHHHHHHHHHHHHHHHHH
ALKRKTDQLARSNAELREALENVRALSGLLPICSRCKKIRDDQGYWKHVETYISLHSDVL
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
FSHGYCPECAEKAMDDLKVQMERYPPRSPSTDETDRW
HCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA