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The map label for this gene is kefB [H]

Identifier: 118580476

GI number: 118580476

Start: 2207270

End: 2209129

Strand: Direct

Name: kefB [H]

Synonym: Ppro_2060

Alternate gene names: 118580476

Gene position: 2207270-2209129 (Clockwise)

Preceding gene: 118580475

Following gene: 118580477

Centisome position: 55.07

GC content: 62.9

Gene sequence:

>1860_bases
ATGACTGACGGCATTCTGGGACAGGCCATCGTCTACCTGGCCGCCGCTCTTCTCTGCGTGCCCCTGGCGAAACGAATCGG
CATGGGCTCGGTGCTCGGCTACCTGCTGGCCGGCGTACTGATCGGCCCCTTCTGCCTCGGCTTCATCGGACATGAGGGTG
AAGATATCATGCACTTCGCCGAGTTCGGCGTGGTGATGATGCTCTTCCTGATCGGTTTGGAACTGGAGCCGGCCCATTTC
TGGAGAATGCGCGGCACCATCCTGGGGCTGGGAACGCTCCAGATCGTGCTGACCACCCTCGCCATGGCCCTGCCGCTCTC
CCTGCTCGGTTTCAGCTGGCAGTCGGCCCTTGCCTCCGGCGTGGCGCTCTCCATGTCATCCACGGCCATCGTACTGCAGA
CGATGAAGGAAAAGGGGTTGACCCAGACCAGGGCCGGAACCGGAGCGTTCGCCGTGCTCCTCTTCCAGGACATTGCCGTC
ATCCCGATCCTCGCCCTGCTCCCCTTCCTGGCCATGGGGGGCAGCACTGCCGACGGCATGGAAAGCACGCTCCTCCAGGG
ACTGCCCGGCTGGATCAGGGCCAGTTCGGTCCTGGTGGCGGTGGTAGGGGTGATCATCCTCGGCCGCTACGTGATCGTCC
CCTTTCTGCGCATCATCACCCGCACCAGCGTGCGGGAACTGTGCATCGCCGCGGCACTGCTGATCATCGTCGGCATCGCC
TTCCTGATGAAACAGGTGGGGCTGAGCCCGGCCCTGGGCGCCTTTCTGGCCGGGGTGCTCCTGGCCAACAGCGAGTTCCG
CCACGAACTGGAAAGCGATATCGAACCGTTCAAGGGGTTGCTCCTGGGGCTGTTCTTCATCGCCGTGGGGGCGTCGATCA
ACTTCGGGCTATTGCTGGACGACCCGGCCAGGCTGTTCCTGATGGTGCTGGCGGTCTTGCTGATCAAGGCCTGCGTGCTT
CTGGCTGCGGGGAAGATCTTCCGTTTCTCCTTTGACCAGAACAGCATCTTCGCCCTGGGGCTCTCCCAGGTGGGCGAATT
CGCCTTTGTGCTTTTCAGCTTCATTAACCGCCTGGGGATACTGGACTCCCCCAGGACCGACCTGTTCATGGCCGTCACCG
CCATCAGCATGACCCTGACCCCGCTCCTCCTGCTGCTCAACGAGCGCTTGGTGCTCCCCCGTTTCGGGACCCTGGAGCAG
GAACGGCGCCAGCCGGACCAGATCGAAAGTGTCCACCAGGTCATCATCGCCGGTTTCAACTCCTTCGGCAGCACCTTGGG
GCGTTTCCTGCGGGCCAACGGCGTGGAAGCCACCATCCTGGACAACGACTCCGACCAGGTGGACCTGCTGCGCAGAATGG
GTTTCAAGGTCTTCTACGGGGATGTCAGCCGCCTGGACATCCTGCGCTCGGCGGGCGCCGAACAGGCCCGTATTCTGGTT
GTAGCCATCGACAACCCCTCCATGACCCTCGATCTGATCGAAAACGTGAAGAAACAGTTCCCCAACCTGACCATCATGGC
CCGCGCCGGAAACAATCTGGATGCCCACGAGTTGCTGGACCTGGATGTACGCCACATCTACCGCGACTTCCTCGACACTG
CCGTGCGGGCCGGGGTGGATGTGTTGGCCGCCCTGGGGCAGCGGCGCTTCAGCGCCACCCGCTCGGCCCAGGACTTCATC
AGGTACGATGAGGATGCAATGGTCAGGCTGGCTCCCCACCGCCATGATCAGTCCTCCTACATCTCCAACACCAGGGAACA
GATCCGGCTGCAGGAGCAACTCCTGGCAAACGACCGCGAACGCAACCGCCTGCGGCACGACCATGCCTGGGACAACACCA
GCCGCGAAATGCCCGACTGA

Upstream 100 bases:

>100_bases
GACTTGCTGATCCGCCTCTCCCAGGCACCGCCTACCCCGGGTGAGTTGTCAGGCATGGAGCGGCTCTACGACTTGAGCGC
TCCCCTCCCCCCCACGCCCC

Downstream 100 bases:

>100_bases
GTCTCCCCGTCCTCCCGACACCTCGGCCACACACCCCCGTGCCCCCATTGAGAGTCCACACTCCAGGCTGTTACATGAGG
CATGTTCGGGACGATTTGTA

Product: potassium efflux system protein

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter; NEM-activatable K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 619; Mature: 618

Protein sequence:

>619_residues
MTDGILGQAIVYLAAALLCVPLAKRIGMGSVLGYLLAGVLIGPFCLGFIGHEGEDIMHFAEFGVVMMLFLIGLELEPAHF
WRMRGTILGLGTLQIVLTTLAMALPLSLLGFSWQSALASGVALSMSSTAIVLQTMKEKGLTQTRAGTGAFAVLLFQDIAV
IPILALLPFLAMGGSTADGMESTLLQGLPGWIRASSVLVAVVGVIILGRYVIVPFLRIITRTSVRELCIAAALLIIVGIA
FLMKQVGLSPALGAFLAGVLLANSEFRHELESDIEPFKGLLLGLFFIAVGASINFGLLLDDPARLFLMVLAVLLIKACVL
LAAGKIFRFSFDQNSIFALGLSQVGEFAFVLFSFINRLGILDSPRTDLFMAVTAISMTLTPLLLLLNERLVLPRFGTLEQ
ERRQPDQIESVHQVIIAGFNSFGSTLGRFLRANGVEATILDNDSDQVDLLRRMGFKVFYGDVSRLDILRSAGAEQARILV
VAIDNPSMTLDLIENVKKQFPNLTIMARAGNNLDAHELLDLDVRHIYRDFLDTAVRAGVDVLAALGQRRFSATRSAQDFI
RYDEDAMVRLAPHRHDQSSYISNTREQIRLQEQLLANDRERNRLRHDHAWDNTSREMPD

Sequences:

>Translated_619_residues
MTDGILGQAIVYLAAALLCVPLAKRIGMGSVLGYLLAGVLIGPFCLGFIGHEGEDIMHFAEFGVVMMLFLIGLELEPAHF
WRMRGTILGLGTLQIVLTTLAMALPLSLLGFSWQSALASGVALSMSSTAIVLQTMKEKGLTQTRAGTGAFAVLLFQDIAV
IPILALLPFLAMGGSTADGMESTLLQGLPGWIRASSVLVAVVGVIILGRYVIVPFLRIITRTSVRELCIAAALLIIVGIA
FLMKQVGLSPALGAFLAGVLLANSEFRHELESDIEPFKGLLLGLFFIAVGASINFGLLLDDPARLFLMVLAVLLIKACVL
LAAGKIFRFSFDQNSIFALGLSQVGEFAFVLFSFINRLGILDSPRTDLFMAVTAISMTLTPLLLLLNERLVLPRFGTLEQ
ERRQPDQIESVHQVIIAGFNSFGSTLGRFLRANGVEATILDNDSDQVDLLRRMGFKVFYGDVSRLDILRSAGAEQARILV
VAIDNPSMTLDLIENVKKQFPNLTIMARAGNNLDAHELLDLDVRHIYRDFLDTAVRAGVDVLAALGQRRFSATRSAQDFI
RYDEDAMVRLAPHRHDQSSYISNTREQIRLQEQLLANDRERNRLRHDHAWDNTSREMPD
>Mature_618_residues
TDGILGQAIVYLAAALLCVPLAKRIGMGSVLGYLLAGVLIGPFCLGFIGHEGEDIMHFAEFGVVMMLFLIGLELEPAHFW
RMRGTILGLGTLQIVLTTLAMALPLSLLGFSWQSALASGVALSMSSTAIVLQTMKEKGLTQTRAGTGAFAVLLFQDIAVI
PILALLPFLAMGGSTADGMESTLLQGLPGWIRASSVLVAVVGVIILGRYVIVPFLRIITRTSVRELCIAAALLIIVGIAF
LMKQVGLSPALGAFLAGVLLANSEFRHELESDIEPFKGLLLGLFFIAVGASINFGLLLDDPARLFLMVLAVLLIKACVLL
AAGKIFRFSFDQNSIFALGLSQVGEFAFVLFSFINRLGILDSPRTDLFMAVTAISMTLTPLLLLLNERLVLPRFGTLEQE
RRQPDQIESVHQVIIAGFNSFGSTLGRFLRANGVEATILDNDSDQVDLLRRMGFKVFYGDVSRLDILRSAGAEQARILVV
AIDNPSMTLDLIENVKKQFPNLTIMARAGNNLDAHELLDLDVRHIYRDFLDTAVRAGVDVLAALGQRRFSATRSAQDFIR
YDEDAMVRLAPHRHDQSSYISNTREQIRLQEQLLANDRERNRLRHDHAWDNTSREMPD

Specific function: Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=373, Percent_Identity=27.0777479892761, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI1786232, Length=616, Percent_Identity=42.5324675324675, Blast_Score=453, Evalue=1e-128,
Organism=Escherichia coli, GI1789749, Length=604, Percent_Identity=41.5562913907285, Blast_Score=438, Evalue=1e-124,
Organism=Escherichia coli, GI1786685, Length=505, Percent_Identity=29.1089108910891, Blast_Score=157, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR020884
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 67780; Mature: 67649

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDGILGQAIVYLAAALLCVPLAKRIGMGSVLGYLLAGVLIGPFCLGFIGHEGEDIMHFA
CCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
EFGVVMMLFLIGLELEPAHFWRMRGTILGLGTLQIVLTTLAMALPLSLLGFSWQSALASG
HHHHHHHHHHHCCCCCHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHC
VALSMSSTAIVLQTMKEKGLTQTRAGTGAFAVLLFQDIAVIPILALLPFLAMGGSTADGM
HHHCCCCHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
ESTLLQGLPGWIRASSVLVAVVGVIILGRYVIVPFLRIITRTSVRELCIAAALLIIVGIA
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLMKQVGLSPALGAFLAGVLLANSEFRHELESDIEPFKGLLLGLFFIAVGASINFGLLLD
HHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEC
DPARLFLMVLAVLLIKACVLLAAGKIFRFSFDQNSIFALGLSQVGEFAFVLFSFINRLGI
CHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHCC
LDSPRTDLFMAVTAISMTLTPLLLLLNERLVLPRFGTLEQERRQPDQIESVHQVIIAGFN
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHH
SFGSTLGRFLRANGVEATILDNDSDQVDLLRRMGFKVFYGDVSRLDILRSAGAEQARILV
HHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCEEEECCHHHHHHHHHCCCCCEEEEE
VAIDNPSMTLDLIENVKKQFPNLTIMARAGNNLDAHELLDLDVRHIYRDFLDTAVRAGVD
EEECCCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VLAALGQRRFSATRSAQDFIRYDEDAMVRLAPHRHDQSSYISNTREQIRLQEQLLANDRE
HHHHHHHHHHHHHHHHHHHHHCCCCHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCHH
RNRLRHDHAWDNTSREMPD
HHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TDGILGQAIVYLAAALLCVPLAKRIGMGSVLGYLLAGVLIGPFCLGFIGHEGEDIMHFA
CCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
EFGVVMMLFLIGLELEPAHFWRMRGTILGLGTLQIVLTTLAMALPLSLLGFSWQSALASG
HHHHHHHHHHHCCCCCHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHC
VALSMSSTAIVLQTMKEKGLTQTRAGTGAFAVLLFQDIAVIPILALLPFLAMGGSTADGM
HHHCCCCHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
ESTLLQGLPGWIRASSVLVAVVGVIILGRYVIVPFLRIITRTSVRELCIAAALLIIVGIA
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLMKQVGLSPALGAFLAGVLLANSEFRHELESDIEPFKGLLLGLFFIAVGASINFGLLLD
HHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEC
DPARLFLMVLAVLLIKACVLLAAGKIFRFSFDQNSIFALGLSQVGEFAFVLFSFINRLGI
CHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHCC
LDSPRTDLFMAVTAISMTLTPLLLLLNERLVLPRFGTLEQERRQPDQIESVHQVIIAGFN
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHH
SFGSTLGRFLRANGVEATILDNDSDQVDLLRRMGFKVFYGDVSRLDILRSAGAEQARILV
HHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCEEEECCHHHHHHHHHCCCCCEEEEE
VAIDNPSMTLDLIENVKKQFPNLTIMARAGNNLDAHELLDLDVRHIYRDFLDTAVRAGVD
EEECCCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VLAALGQRRFSATRSAQDFIRYDEDAMVRLAPHRHDQSSYISNTREQIRLQEQLLANDRE
HHHHHHHHHHHHHHHHHHHHHCCCCHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCHH
RNRLRHDHAWDNTSREMPD
HHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA