Click here to switch to the map view.

The map label for this gene is prfB [H]

Identifier: 118580454

GI number: 118580454

Start: 2189113

End: 2190198

Strand: Direct

Name: prfB [H]

Synonym: Ppro_2038

Alternate gene names: 118580454

Gene position: 2189113-2190198 (Clockwise)

Preceding gene: 118580453

Following gene: 118580456

Centisome position: 54.62

GC content: 61.23

Gene sequence:

>1086_bases
TTGAGGAGCGTATCGCCAAGCTCCGGGGGTCTCTTTGACGTAGATGGCAAACGGGAATCGATCCAGGAGATCGAATCCAA
GATCGCCGCCCCCGGTTTCTGGGACAACGCGGACTCATCCCAGGGAATCCTCAAGGAGCGAACCAGCCTGGAAAAGATCG
TGCAGCTGTGGGACTCCCTCGTCCGTCAGCAGGAGGATGTGCGGGTGATGATCGAACTGGGCGAGGAGGAGGCTGACGAG
GAAACACTGAAAGAAGTAGGGGAGATGAACAACCGCCTGCAGCAGGGTGTGGAGGCGGCGGAGTTCCAACGTATGCTCTC
CGGCGAACATGACCGCAACTCCTGCTTCATCTCCATCAACCCGGGAGCCGGCGGCACCGAATCCCAGGATTGGGCCGAAA
TGCTGCTGCGCATGTACCTGCGTTACTGCGAAAAGAAGGGGTGGAAGACCAGCATCACCGACTACCAGTCCGGAGACGAG
GCCGGCATCAAGTCGGCCACCTTCAGCGTCAGCGGCGAGTATGCCTACGGCTACCTCAAGGCCGAGGCGGGCATTCACCG
CCTGGTGCGCATCTCCCCCTTTGACAGCAACGCCCGCCGCCACACCTCCTTTGCCTCGGTCTACGCATTCCCGGAGATAG
AGGAGGAGGACATCGACATCAAGATCGCCGATTCCGACCTGCGGGTGGACACCTTTCGCTCGGGCGGCGCCGGTGGGCAG
CATGTGAACACCACCGACTCGGCCGTTCGCATCACCCACCTTCCCACGAACATCGTCGTGGCCTGCCAGAGTGAACGGAG
CCAGATCCTTAACCGCGCAACAGCCATGAAGGTGCTGCGGGCGAAACTGTACGAGAAAGAGGTGCAGGAGCGGACGGCCA
GGGCATCGGAGATCGCCAGCGAGAAAAAGGAGATCGGTTGGGGAAGCCAAATCCGCTCCTATGTGCTGCACCCCTACAAG
ATGGTCAAGGACCTGCGCACCGGAGTGGAGTCGGGCAACCCGGATGCGGTGCTGGACGGCGCCCTGGAGGAGTTCGTGGT
AGCGTATCTGATGGGAGTCAGGCGACAGGCCGGAGATGCGGAGTAA

Upstream 100 bases:

>100_bases
GCGGGGAGCACATCAGTGTCCGCCCGCCGCCGCTGGCTCAGCACACCACCAGAGAAGGAGCACACCATGTTTCGTGAAGA
AGTAGCCCGTTTGAACAACT

Downstream 100 bases:

>100_bases
GGCCTGTCGCTGAAAAAGATCAGGCCGTATGACTCTTGGTCATACGGCCTGATGGAAACCATCATCGTGTGGGGCCGTCC
TGTTTCAAACTGTCGTGTCC

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MRSVSPSSGGLFDVDGKRESIQEIESKIAAPGFWDNADSSQGILKERTSLEKIVQLWDSLVRQQEDVRVMIELGEEEADE
ETLKEVGEMNNRLQQGVEAAEFQRMLSGEHDRNSCFISINPGAGGTESQDWAEMLLRMYLRYCEKKGWKTSITDYQSGDE
AGIKSATFSVSGEYAYGYLKAEAGIHRLVRISPFDSNARRHTSFASVYAFPEIEEEDIDIKIADSDLRVDTFRSGGAGGQ
HVNTTDSAVRITHLPTNIVVACQSERSQILNRATAMKVLRAKLYEKEVQERTARASEIASEKKEIGWGSQIRSYVLHPYK
MVKDLRTGVESGNPDAVLDGALEEFVVAYLMGVRRQAGDAE

Sequences:

>Translated_361_residues
MRSVSPSSGGLFDVDGKRESIQEIESKIAAPGFWDNADSSQGILKERTSLEKIVQLWDSLVRQQEDVRVMIELGEEEADE
ETLKEVGEMNNRLQQGVEAAEFQRMLSGEHDRNSCFISINPGAGGTESQDWAEMLLRMYLRYCEKKGWKTSITDYQSGDE
AGIKSATFSVSGEYAYGYLKAEAGIHRLVRISPFDSNARRHTSFASVYAFPEIEEEDIDIKIADSDLRVDTFRSGGAGGQ
HVNTTDSAVRITHLPTNIVVACQSERSQILNRATAMKVLRAKLYEKEVQERTARASEIASEKKEIGWGSQIRSYVLHPYK
MVKDLRTGVESGNPDAVLDGALEEFVVAYLMGVRRQAGDAE
>Mature_361_residues
MRSVSPSSGGLFDVDGKRESIQEIESKIAAPGFWDNADSSQGILKERTSLEKIVQLWDSLVRQQEDVRVMIELGEEEADE
ETLKEVGEMNNRLQQGVEAAEFQRMLSGEHDRNSCFISINPGAGGTESQDWAEMLLRMYLRYCEKKGWKTSITDYQSGDE
AGIKSATFSVSGEYAYGYLKAEAGIHRLVRISPFDSNARRHTSFASVYAFPEIEEEDIDIKIADSDLRVDTFRSGGAGGQ
HVNTTDSAVRITHLPTNIVVACQSERSQILNRATAMKVLRAKLYEKEVQERTARASEIASEKKEIGWGSQIRSYVLHPYK
MVKDLRTGVESGNPDAVLDGALEEFVVAYLMGVRRQAGDAE

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=247, Percent_Identity=38.8663967611336, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI34577120, Length=222, Percent_Identity=39.6396396396396, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI166795305, Length=151, Percent_Identity=40.3973509933775, Blast_Score=121, Evalue=1e-27,
Organism=Escherichia coli, GI2367172, Length=341, Percent_Identity=50.733137829912, Blast_Score=360, Evalue=1e-100,
Organism=Escherichia coli, GI1787462, Length=239, Percent_Identity=40.5857740585774, Blast_Score=175, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI17542784, Length=212, Percent_Identity=35.8490566037736, Blast_Score=116, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6321295, Length=242, Percent_Identity=35.9504132231405, Blast_Score=136, Evalue=5e-33,
Organism=Drosophila melanogaster, GI19921226, Length=219, Percent_Identity=41.5525114155251, Blast_Score=167, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40235; Mature: 40235

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSVSPSSGGLFDVDGKRESIQEIESKIAAPGFWDNADSSQGILKERTSLEKIVQLWDSL
CCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VRQQEDVRVMIELGEEEADEETLKEVGEMNNRLQQGVEAAEFQRMLSGEHDRNSCFISIN
HCCHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEEEEEC
PGAGGTESQDWAEMLLRMYLRYCEKKGWKTSITDYQSGDEAGIKSATFSVSGEYAYGYLK
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEEE
AEAGIHRLVRISPFDSNARRHTSFASVYAFPEIEEEDIDIKIADSDLRVDTFRSGGAGGQ
HHHHHHEEEEECCCCCCCHHHHHHHHEEECCCCCCCCCEEEEECCCCEEEECCCCCCCCC
HVNTTDSAVRITHLPTNIVVACQSERSQILNRATAMKVLRAKLYEKEVQERTARASEIAS
CCCCCCCEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKKEIGWGSQIRSYVLHPYKMVKDLRTGVESGNPDAVLDGALEEFVVAYLMGVRRQAGDA
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
E
C
>Mature Secondary Structure
MRSVSPSSGGLFDVDGKRESIQEIESKIAAPGFWDNADSSQGILKERTSLEKIVQLWDSL
CCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VRQQEDVRVMIELGEEEADEETLKEVGEMNNRLQQGVEAAEFQRMLSGEHDRNSCFISIN
HCCHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEEEEEC
PGAGGTESQDWAEMLLRMYLRYCEKKGWKTSITDYQSGDEAGIKSATFSVSGEYAYGYLK
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEEE
AEAGIHRLVRISPFDSNARRHTSFASVYAFPEIEEEDIDIKIADSDLRVDTFRSGGAGGQ
HHHHHHEEEEECCCCCCCHHHHHHHHEEECCCCCCCCCEEEEECCCCEEEECCCCCCCCC
HVNTTDSAVRITHLPTNIVVACQSERSQILNRATAMKVLRAKLYEKEVQERTARASEIAS
CCCCCCCEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKKEIGWGSQIRSYVLHPYKMVKDLRTGVESGNPDAVLDGALEEFVVAYLMGVRRQAGDA
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA