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The map label for this gene is yiaE [C]

Identifier: 118580448

GI number: 118580448

Start: 2181796

End: 2182791

Strand: Reverse

Name: yiaE [C]

Synonym: Ppro_2032

Alternate gene names: 118580448

Gene position: 2182791-2181796 (Counterclockwise)

Preceding gene: 118580455

Following gene: 118580445

Centisome position: 54.46

GC content: 63.35

Gene sequence:

>996_bases
ATGAGCGAGAAGAGCAGAATCGTGATTCTGGACGGCCATACCATCAACCCCGGCGATGCCAGCTGGGAACCGCTGGAGGC
ACTGGGGGAGTGTGTGATCCATGGGCGTACTCCGCCGGAGTTGATCGTCGAACGTGCCCGGGAGGCGCGCGTGATCCTGA
CCAGCAAGTGCACTTTGAGCGCCGCTGTTCTGGCGGCATTGCCGAAGCTCGAATATTTATCGGTCCTGGCCACCGGCTAC
AACAACGTCGACATTGTTGCTGCGGGGCGGCGGGGCATTCCGGTCTCCAACGTTCCGGCCTATGCAACGGAAACGGTTGC
CCAGGCTGTATTTGCCCTGCTGCTGGAGTTGACCTCGGCTGTTGGCGCCCATGCTGCCGCGGTAAGGGGGGGGGAGTGGG
TGGACTGTCCCGATTTCTGTTTCTACCGGAAAACCATCCTGGAGTTGAATGGCCTGACCCTGGGGGTCGTGGGCTACGGC
TCGACCGGGCAGGCTGTCGCGCGCATCGCCAACGCGTTCGGCATGCGAGTCCTCGTCCATGCCCAGCGCATTCCGCGTCA
CCTGGGACAATTGCCGGTGCGTTTGGTTCCCCTGGAGGAGCTTTTCGAGACCGCAGACGTGATCAGCCTCAACTGTCCGC
AGACCAGGGAAAACAGCGGATTTGTCAATGCCGGGCTCCTGGCGAGAATGAAACCGAGCTCCCTTCTGATCAATACGTCC
AGGGGAGGCCTGATAAACGAGGCCGACCTGGACCGGGCGTTGCGTGACGGTATCATCGCCGGAGCGGGACTGGATGTGCT
TGCCCACGAACCGCCCCGTGGCGACAACCCGCTGCTCTCGGCCCCCAACTGCATCATCACCCCCCACCTTGCCTGGGCCT
CGCTGGCGGCGCGCACAAGGCTGGTGGGGATGGCAGCCGCCAACGTGGCCTCGTTTCTGGCCGCTGCGCCGGTCAATGTG
ATCAACGGCGAGTACCTGCCCGCAGCACGCGGCTAG

Upstream 100 bases:

>100_bases
GCCGGGGGGAAATAGCGTGAGAACTGGTCGTGACGCTTGACTTTCCGGGAGATAACCCTACAATCTGGCACTATATTTTC
GGAGGAAGGATCGTGCTGCC

Downstream 100 bases:

>100_bases
CAGATAAAACTCCTTCCCCGTTTAGGCCCTGCTTCCCTATGAATCGAACATAAGCTTCCGTTCCAGGTCCATGGAGTGGG
TCAGACGCTTGGCATCCTCG

Product: NAD-binding D-isomer specific 2-hydroxyacid dehydrogenase

Products: hydroxypyruvate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MSEKSRIVILDGHTINPGDASWEPLEALGECVIHGRTPPELIVERAREARVILTSKCTLSAAVLAALPKLEYLSVLATGY
NNVDIVAAGRRGIPVSNVPAYATETVAQAVFALLLELTSAVGAHAAAVRGGEWVDCPDFCFYRKTILELNGLTLGVVGYG
STGQAVARIANAFGMRVLVHAQRIPRHLGQLPVRLVPLEELFETADVISLNCPQTRENSGFVNAGLLARMKPSSLLINTS
RGGLINEADLDRALRDGIIAGAGLDVLAHEPPRGDNPLLSAPNCIITPHLAWASLAARTRLVGMAAANVASFLAAAPVNV
INGEYLPAARG

Sequences:

>Translated_331_residues
MSEKSRIVILDGHTINPGDASWEPLEALGECVIHGRTPPELIVERAREARVILTSKCTLSAAVLAALPKLEYLSVLATGY
NNVDIVAAGRRGIPVSNVPAYATETVAQAVFALLLELTSAVGAHAAAVRGGEWVDCPDFCFYRKTILELNGLTLGVVGYG
STGQAVARIANAFGMRVLVHAQRIPRHLGQLPVRLVPLEELFETADVISLNCPQTRENSGFVNAGLLARMKPSSLLINTS
RGGLINEADLDRALRDGIIAGAGLDVLAHEPPRGDNPLLSAPNCIITPHLAWASLAARTRLVGMAAANVASFLAAAPVNV
INGEYLPAARG
>Mature_330_residues
SEKSRIVILDGHTINPGDASWEPLEALGECVIHGRTPPELIVERAREARVILTSKCTLSAAVLAALPKLEYLSVLATGYN
NVDIVAAGRRGIPVSNVPAYATETVAQAVFALLLELTSAVGAHAAAVRGGEWVDCPDFCFYRKTILELNGLTLGVVGYGS
TGQAVARIANAFGMRVLVHAQRIPRHLGQLPVRLVPLEELFETADVISLNCPQTRENSGFVNAGLLARMKPSSLLINTSR
GGLINEADLDRALRDGIIAGAGLDVLAHEPPRGDNPLLSAPNCIITPHLAWASLAARTRLVGMAAANVASFLAAAPVNVI
NGEYLPAARG

Specific function: Unknown

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=294, Percent_Identity=31.9727891156463, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI145580578, Length=285, Percent_Identity=33.3333333333333, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI4557499, Length=285, Percent_Identity=33.3333333333333, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI61743967, Length=288, Percent_Identity=32.6388888888889, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI4557497, Length=288, Percent_Identity=32.6388888888889, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI145580575, Length=264, Percent_Identity=34.8484848484849, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI6912396, Length=258, Percent_Identity=31.7829457364341, Blast_Score=128, Evalue=8e-30,
Organism=Escherichia coli, GI87082289, Length=272, Percent_Identity=35.2941176470588, Blast_Score=137, Evalue=9e-34,
Organism=Escherichia coli, GI1789279, Length=307, Percent_Identity=31.5960912052117, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1787645, Length=267, Percent_Identity=32.2097378277154, Blast_Score=111, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI17532191, Length=291, Percent_Identity=31.2714776632302, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI25147481, Length=264, Percent_Identity=33.3333333333333, Blast_Score=114, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6320925, Length=277, Percent_Identity=30.3249097472924, Blast_Score=125, Evalue=8e-30,
Organism=Saccharomyces cerevisiae, GI6322116, Length=277, Percent_Identity=28.8808664259928, Blast_Score=120, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6324964, Length=325, Percent_Identity=26.1538461538462, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324055, Length=249, Percent_Identity=27.3092369477912, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324980, Length=154, Percent_Identity=29.2207792207792, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24646446, Length=280, Percent_Identity=34.6428571428571, Blast_Score=134, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24646448, Length=280, Percent_Identity=34.6428571428571, Blast_Score=134, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24646452, Length=280, Percent_Identity=34.6428571428571, Blast_Score=134, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24646450, Length=280, Percent_Identity=34.6428571428571, Blast_Score=134, Evalue=6e-32,
Organism=Drosophila melanogaster, GI28574286, Length=335, Percent_Identity=31.6417910447761, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI62472511, Length=278, Percent_Identity=34.5323741007194, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24585516, Length=263, Percent_Identity=28.1368821292776, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI45552429, Length=262, Percent_Identity=27.4809160305344, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24585514, Length=262, Percent_Identity=27.4809160305344, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI28574282, Length=262, Percent_Identity=27.4809160305344, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI45551003, Length=262, Percent_Identity=27.4809160305344, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI28574284, Length=262, Percent_Identity=27.4809160305344, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI28571528, Length=252, Percent_Identity=30.5555555555556, Blast_Score=111, Evalue=7e-25,
Organism=Drosophila melanogaster, GI19921140, Length=287, Percent_Identity=28.5714285714286, Blast_Score=98, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: 1.1.1.29

Molecular weight: Translated: 34936; Mature: 34805

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKSRIVILDGHTINPGDASWEPLEALGECVIHGRTPPELIVERAREARVILTSKCTLS
CCCCCEEEEEECCEECCCCCCCCHHHHHHHHEECCCCCHHHHHHHHHCCEEEEECCHHHH
AAVLAALPKLEYLSVLATGYNNVDIVAAGRRGIPVSNVPAYATETVAQAVFALLLELTSA
HHHHHHHCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
VGAHAAAVRGGEWVDCPDFCFYRKTILELNGLTLGVVGYGSTGQAVARIANAFGMRVLVH
HHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHH
AQRIPRHLGQLPVRLVPLEELFETADVISLNCPQTRENSGFVNAGLLARMKPSSLLINTS
HHHHHHHHCCCCEEEECHHHHHCCCCEEECCCCCCCCCCCCEECCEEEEECCCEEEEECC
RGGLINEADLDRALRDGIIAGAGLDVLAHEPPRGDNPLLSAPNCIITPHLAWASLAARTR
CCCCCCHHHHHHHHHCCEEECCCCCEEECCCCCCCCCCCCCCCEEEECCHHHHHHHHHHH
LVGMAAANVASFLAAAPVNVINGEYLPAARG
HHHHHHHHHHHHHHHCCHHHCCCCCCCCCCC
>Mature Secondary Structure 
SEKSRIVILDGHTINPGDASWEPLEALGECVIHGRTPPELIVERAREARVILTSKCTLS
CCCCEEEEEECCEECCCCCCCCHHHHHHHHEECCCCCHHHHHHHHHCCEEEEECCHHHH
AAVLAALPKLEYLSVLATGYNNVDIVAAGRRGIPVSNVPAYATETVAQAVFALLLELTSA
HHHHHHHCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
VGAHAAAVRGGEWVDCPDFCFYRKTILELNGLTLGVVGYGSTGQAVARIANAFGMRVLVH
HHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHH
AQRIPRHLGQLPVRLVPLEELFETADVISLNCPQTRENSGFVNAGLLARMKPSSLLINTS
HHHHHHHHCCCCEEEECHHHHHCCCCEEECCCCCCCCCCCCEECCEEEEECCCEEEEECC
RGGLINEADLDRALRDGIIAGAGLDVLAHEPPRGDNPLLSAPNCIITPHLAWASLAARTR
CCCCCCHHHHHHHHHCCEEECCCCCEEECCCCCCCCCCCCCCCEEEECCHHHHHHHHHHH
LVGMAAANVASFLAAAPVNVINGEYLPAARG
HHHHHHHHHHHHHHHCCHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-glycerate; NAD+

Specific reaction: (R)-glycerate + NAD+ = hydroxypyruvate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]