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The map label for this gene is gcvH [H]

Identifier: 118580437

GI number: 118580437

Start: 2170960

End: 2171346

Strand: Reverse

Name: gcvH [H]

Synonym: Ppro_2021

Alternate gene names: 118580437

Gene position: 2171346-2170960 (Counterclockwise)

Preceding gene: 118580438

Following gene: 118580436

Centisome position: 54.18

GC content: 57.36

Gene sequence:

>387_bases
ATGGACTTTCCTGAAGAGCTCAAGTATTCCCGTGAGCATGTCTGGGTTCGGATCGAGAATGGAACGGCGGTCATCGGCAT
CACGGATTTTGCCCAGAATGAGTTGGGCATCATCTTGGCGCTGGAGTTGCCCGATGAGGGGGATGACGTCGAGCAGGACG
ATTCGTTCGGCTCCATCGAGGGGAGGAAGACCGTTGCCGAGTTGTATGCCCCCTTCAGTGGCACCATTGAGTCGGTCAAT
GCGGAGGTTCTGGACAACCCGGGGCTGCTCAACGATGACCCGTACGATGGTGGCTGGTTGCTGGAGGTGACGCTGGACGA
CCCCGATGAGCTGAAGGGGTTGATGACGGCCGATGACTATGCCGAGTATGTGGAGAACAGGGATTGA

Upstream 100 bases:

>100_bases
GACCACCATCTTCTTCCAGAAGAGGATCATGACCAACAAGGATTTTATCGAAGGAAACATAGATACATCATTTCTTGAGA
GAATCGTGCTGGAGTAGACT

Downstream 100 bases:

>100_bases
TCGACCTGCTACATGGCGGGGCAGGGGTGCGCGGTATCCCTCCCCGCCGTTTTCATACAGGGATGCTGGCGTGAGATCGG
TGATGGTTGTGATAGCACTG

Product: glycine cleavage system H protein

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 128; Mature: 128

Protein sequence:

>128_residues
MDFPEELKYSREHVWVRIENGTAVIGITDFAQNELGIILALELPDEGDDVEQDDSFGSIEGRKTVAELYAPFSGTIESVN
AEVLDNPGLLNDDPYDGGWLLEVTLDDPDELKGLMTADDYAEYVENRD

Sequences:

>Translated_128_residues
MDFPEELKYSREHVWVRIENGTAVIGITDFAQNELGIILALELPDEGDDVEQDDSFGSIEGRKTVAELYAPFSGTIESVN
AEVLDNPGLLNDDPYDGGWLLEVTLDDPDELKGLMTADDYAEYVENRD
>Mature_128_residues
MDFPEELKYSREHVWVRIENGTAVIGITDFAQNELGIILALELPDEGDDVEQDDSFGSIEGRKTVAELYAPFSGTIESVN
AEVLDNPGLLNDDPYDGGWLLEVTLDDPDELKGLMTADDYAEYVENRD

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=119, Percent_Identity=39.4957983193277, Blast_Score=112, Evalue=6e-26,
Organism=Homo sapiens, GI89057342, Length=119, Percent_Identity=38.655462184874, Blast_Score=110, Evalue=4e-25,
Organism=Escherichia coli, GI1789271, Length=127, Percent_Identity=40.1574803149606, Blast_Score=99, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17507493, Length=115, Percent_Identity=40, Blast_Score=113, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17551294, Length=115, Percent_Identity=34.7826086956522, Blast_Score=97, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6319272, Length=121, Percent_Identity=36.3636363636364, Blast_Score=99, Evalue=2e-22,
Organism=Drosophila melanogaster, GI17865652, Length=125, Percent_Identity=39.2, Blast_Score=111, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 14257; Mature: 14257

Theoretical pI: Translated: 3.53; Mature: 3.53

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFPEELKYSREHVWVRIENGTAVIGITDFAQNELGIILALELPDEGDDVEQDDSFGSIE
CCCCHHHCCCCCEEEEEECCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCC
GRKTVAELYAPFSGTIESVNAEVLDNPGLLNDDPYDGGWLLEVTLDDPDELKGLMTADDY
CHHHHHHHHCCCCCCEECCCEEECCCCCCCCCCCCCCCEEEEEEECCHHHHCEECCCHHH
AEYVENRD
HHHHCCCC
>Mature Secondary Structure
MDFPEELKYSREHVWVRIENGTAVIGITDFAQNELGIILALELPDEGDDVEQDDSFGSIE
CCCCHHHCCCCCEEEEEECCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCC
GRKTVAELYAPFSGTIESVNAEVLDNPGLLNDDPYDGGWLLEVTLDDPDELKGLMTADDY
CHHHHHHHHCCCCCCEECCCEEECCCCCCCCCCCCCCCEEEEEEECCHHHHCEECCCHHH
AEYVENRD
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA