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The map label for this gene is ispB [H]

Identifier: 118580423

GI number: 118580423

Start: 2155261

End: 2156229

Strand: Reverse

Name: ispB [H]

Synonym: Ppro_2007

Alternate gene names: 118580423

Gene position: 2156229-2155261 (Counterclockwise)

Preceding gene: 118580424

Following gene: 118580422

Centisome position: 53.8

GC content: 63.26

Gene sequence:

>969_bases
ATGCAAGCTGCACTGAATATCATCGGCGACGATTTGAAGCGAGTCGAACAGCAATTCCGCACGGACCTGGAGTCCGATGT
TCCCCTGATCCGCAAGGTTGGCGAGTACGTGCTCTCCAGCGGCGGCAAACGCGTCAGGCCGGCACTGCTGCTTTTGGCTG
CTCGCCTGTGCGACTACCAGGGAGACAAGGCCGTTCCCCTGGCCAGCGTCATAGAGTTCATCCACACCGCCACCCTGCTC
CACGACGACGTTGTGGACAGCGCCACCCTGCGGCGCGGCCTGGCCTCGGCCAACACCCTCTGGGGCAATGAAGCCTCCGT
GCTGGTGGGCGACTTCCTCTTCTCCAAATCCTTCTCCCTGATGGTTGCGGTCGGCAGCTTGGATATCCTCAAGGTGCTCT
CCGACGCGACCACGGTGATCGCCGAGGGCGAGGTCATGCAGCTGCTCTGCACCGGCGAACTGGAGCTGACCGAGGAGCGC
TACATCGATGTGGTACGCTCCAAGACCGCCATTCTCATGTCCGCGGCCTGCGAGGCCGGGGCGATTCTGGGGGGAGCGGC
GCCGGAGCGGCGCCAGGCACTCTCCGATTTCGGCATGAATCTGGGCATCGCTTTCCAGCTCATGGACGACACGCTGGACT
ACATCGCCACCGAAGAGGAGTTCGGCAAGAGCATCGGCCACGACCTGGAGGAGGGGAAGATCACCATGCCCCTGATCCAT
GTCCTGCGTCACTGCACTCCCTCGGAGCGCGACCAGATCGCGACAGTTGTTGAAAAGGACGAGATGACCCTGGACGAATT
CCGCCAGGTGTCGGGGCTGGTGCAGCGCTACGGCGGCATCGACTATACCGTGGATGCCGCGCGGCGCTACATCCAGGGCT
GCGCTGACTCGTTGAGCCTGTTCGCTTCCGGCCCGGTGGCGGATGCGCTGCTGGATCTGGCCGGGTATGTGGTTACCCGC
AGCAAGTGA

Upstream 100 bases:

>100_bases
CCGCCTCCCCGCTGACGGCGCTGTAACTGCCAGAAATACTTTGACAAAACCGTTTTGATTCCAGTACTATGACGAATCTT
TTTCCCACAAGGACGACCGG

Downstream 100 bases:

>100_bases
GAAACCCGGTTCGCCACGGGGAGGGTGCATGAAGAAACGTCTCTGTCTGCTGGCGCTGGCAATCCTGGCCGCGCTGACCA
TGGCCTGCCAGCGGAACGAA

Product: trans-hexaprenyltranstransferase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MQAALNIIGDDLKRVEQQFRTDLESDVPLIRKVGEYVLSSGGKRVRPALLLLAARLCDYQGDKAVPLASVIEFIHTATLL
HDDVVDSATLRRGLASANTLWGNEASVLVGDFLFSKSFSLMVAVGSLDILKVLSDATTVIAEGEVMQLLCTGELELTEER
YIDVVRSKTAILMSAACEAGAILGGAAPERRQALSDFGMNLGIAFQLMDDTLDYIATEEEFGKSIGHDLEEGKITMPLIH
VLRHCTPSERDQIATVVEKDEMTLDEFRQVSGLVQRYGGIDYTVDAARRYIQGCADSLSLFASGPVADALLDLAGYVVTR
SK

Sequences:

>Translated_322_residues
MQAALNIIGDDLKRVEQQFRTDLESDVPLIRKVGEYVLSSGGKRVRPALLLLAARLCDYQGDKAVPLASVIEFIHTATLL
HDDVVDSATLRRGLASANTLWGNEASVLVGDFLFSKSFSLMVAVGSLDILKVLSDATTVIAEGEVMQLLCTGELELTEER
YIDVVRSKTAILMSAACEAGAILGGAAPERRQALSDFGMNLGIAFQLMDDTLDYIATEEEFGKSIGHDLEEGKITMPLIH
VLRHCTPSERDQIATVVEKDEMTLDEFRQVSGLVQRYGGIDYTVDAARRYIQGCADSLSLFASGPVADALLDLAGYVVTR
SK
>Mature_322_residues
MQAALNIIGDDLKRVEQQFRTDLESDVPLIRKVGEYVLSSGGKRVRPALLLLAARLCDYQGDKAVPLASVIEFIHTATLL
HDDVVDSATLRRGLASANTLWGNEASVLVGDFLFSKSFSLMVAVGSLDILKVLSDATTVIAEGEVMQLLCTGELELTEER
YIDVVRSKTAILMSAACEAGAILGGAAPERRQALSDFGMNLGIAFQLMDDTLDYIATEEEFGKSIGHDLEEGKITMPLIH
VLRHCTPSERDQIATVVEKDEMTLDEFRQVSGLVQRYGGIDYTVDAARRYIQGCADSLSLFASGPVADALLDLAGYVVTR
SK

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=324, Percent_Identity=29.9382716049383, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI4758430, Length=255, Percent_Identity=23.5294117647059, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI83700220, Length=255, Percent_Identity=23.5294117647059, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI1789578, Length=316, Percent_Identity=43.6708860759494, Blast_Score=270, Evalue=1e-73,
Organism=Escherichia coli, GI1786623, Length=244, Percent_Identity=28.6885245901639, Blast_Score=78, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17505681, Length=239, Percent_Identity=33.4728033472803, Blast_Score=148, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6319475, Length=307, Percent_Identity=30.2931596091205, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6325188, Length=223, Percent_Identity=22.8699551569507, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24651612, Length=332, Percent_Identity=29.5180722891566, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI281365769, Length=278, Percent_Identity=21.5827338129496, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24660002, Length=278, Percent_Identity=21.5827338129496, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 34962; Mature: 34962

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAALNIIGDDLKRVEQQFRTDLESDVPLIRKVGEYVLSSGGKRVRPALLLLAARLCDYQ
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
GDKAVPLASVIEFIHTATLLHDDVVDSATLRRGLASANTLWGNEASVLVGDFLFSKSFSL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEE
MVAVGSLDILKVLSDATTVIAEGEVMQLLCTGELELTEERYIDVVRSKTAILMSAACEAG
EEEECHHHHHHHHHHHHHHHCCCHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
AILGGAAPERRQALSDFGMNLGIAFQLMDDTLDYIATEEEFGKSIGHDLEEGKITMPLIH
CCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCHHHHHHHHCCCHHCCCCHHHHHH
VLRHCTPSERDQIATVVEKDEMTLDEFRQVSGLVQRYGGIDYTVDAARRYIQGCADSLSL
HHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHH
FASGPVADALLDLAGYVVTRSK
HCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQAALNIIGDDLKRVEQQFRTDLESDVPLIRKVGEYVLSSGGKRVRPALLLLAARLCDYQ
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
GDKAVPLASVIEFIHTATLLHDDVVDSATLRRGLASANTLWGNEASVLVGDFLFSKSFSL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEE
MVAVGSLDILKVLSDATTVIAEGEVMQLLCTGELELTEERYIDVVRSKTAILMSAACEAG
EEEECHHHHHHHHHHHHHHHCCCHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
AILGGAAPERRQALSDFGMNLGIAFQLMDDTLDYIATEEEFGKSIGHDLEEGKITMPLIH
CCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCHHHHHHHHCCCHHCCCCHHHHHH
VLRHCTPSERDQIATVVEKDEMTLDEFRQVSGLVQRYGGIDYTVDAARRYIQGCADSLSL
HHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHH
FASGPVADALLDLAGYVVTRSK
HCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]