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The map label for this gene is kinA [H]

Identifier: 118580419

GI number: 118580419

Start: 2151487

End: 2153448

Strand: Reverse

Name: kinA [H]

Synonym: Ppro_2003

Alternate gene names: 118580419

Gene position: 2153448-2151487 (Counterclockwise)

Preceding gene: 118580420

Following gene: 118580418

Centisome position: 53.73

GC content: 58.82

Gene sequence:

>1962_bases
GTGGATGCTTCACGGAACAGTGGCTGCTCATCGCCGAAACAGCTGATGCACAGCATCCTGCAGGCGATTCCCGACCTGAT
CTCCGTGGTCGATACTGATTTCAAGATCGTCTACAGCAACTGGAGGGGAGGGCATGAGTATGCCGCTCCCAACCGGCGCA
AGCAGAACAGGCACTGCTATGAGGTCTATTATCCCGATCTGAACGGCCGCTGCGACCCCTGCCATCTGGTGCAGGTATTC
GAAACCGGCATGAGCATCGTCGCCGAGAAATTCAATCCCAGGGTCGGCTATCTGGAGATTCACGCCTTCCCCATTTTCGA
TGCATCCGGCACTGTGGTCATGGCCGGCGAATACCTGCGCAACATATCCGACCGCAAGCATGCCGAAGACGCCCTTCGCG
GGGCAAACCAGATGCTGGAAGCGCTGGTCAACGCATCGCCGCCTGCCATACTCACCCTGGACCTGGACCTGAACCTGACT
CTCTGGAACCCGGCTGCCGAGCGGATGTTTGGCTGGAAGATAGACGAGGTGCTCGGCAAATCCTATCCCATCGTCTCCAT
CGAACTGATGGAGGAACTCAAGGAGAATATCCGCCGCCTGAACCGGGGGGAGGAGCGTCAATCCATGGAGACGAGGCGGC
TGCACCGGGATGGCCATTTCATCGACGTCAGCCTGTCAACGGCCCCCATGCTGAACAGCGAGGGGGGCACCATCGGGTAC
GTGGCGATCATGGCCGATATCAGCGAGCGCAAGCAGGCGCAGCAGGCGCTCAGGGAATCCGAGGCCAATTACCGCGCCAT
TTTCGATGCGGCCAATGACGCCACCTTCGTGTTCGACGTGGAAACCGGCGCCATGCTGGATGTCAACCTGAAGATGTGCC
AGATGTACGGCTACCTGCGCGAGGAGGTTTTGCTGCTCAACGTGGAACAGTTGAGCGCCGGATTCCCCCCCTATACTTTC
CAGGATTTGATGAAACTGATCTGGAAGACCAAGAGAGACCAGTCCCACCTGTTCGAATGGCTGGCCAAGGACAGGTCCGG
ACGCCTCTTCTGGGTCGAGGTGAACATGAAAGGAGCCGTGATCGGGGGGGAGTACAAGGTCCTGGCGGTGGTGCGTGACA
TATCGGCGCGAAAGGCCGCCGAAGAGGAGAACAGGATCATGCAGGAGCGCCTCCTCCAGGCCAACAAGATGGCGGCGATC
GGCACCCTGGCCTCGGGAATCGCCCACGAGATCAACAATCCCAACAACTATATCCTCTCCAATGCCCAGTTCCTGAGCGA
CATTTGGCCCGAGATCAACCGGGTTCTCACCCGCTACGCCGAGGAGAACGGCGAATTCTTCCTGGGCAAGCTGGGCTACG
GCGAGGCGGCGGCCATGATCCCCAAAATGCTCACCGGTCTTACGGAAGGGGCCTACCGTATCAAGGGGATCGTCACCGGT
CTGAAGGATTTCGCGCGTCAGGAAAAGACCCGTCTCGACAAGCCGGTGGACATCAACAAGGTCATCGAGGCGGCGCTCTC
CATGCTGCACAACAAGATCAAGCAGCACACCGACAATTTCAGCTGCAGCCTGGACAAGGCGCTTCCGCCGGTCAGGGGGC
GTTTCCGCCAGCTGGAACAGGTGATTGTCAACCTGACCATGAACGCCCTTGAGTCGCTTCCCTCCCGGGACCGGGCGGTT
TCCATCCAGACCTCCTATTCCCAGTACCTGGAACAGATCACCATCAGGGTCGAGGATCAGGGGATCGGCATGACCGAGGA
GGTGCAGAAGGCCATTTTCGATCCCTTTTTCACCACCAAGCTGGACCGGGGCGGAACCGGCCTGGGGCTTTCCATCAACT
TCACCATTGTCAAAGAGCACAACGGCATCATCGAGTGCGATTCCAGTCCGGGGAAGGGCACCGTGTTTTATGTCAGAATA
CCGGCCCTGCGTTTCGAACCTGAGGAGAACGGGTTACCATGA

Upstream 100 bases:

>100_bases
CGCCTTACGGCCATAGGCGCTGGGTAGTACCCCTGTATGGTTCCGTCTTGCGCGGATTAGGGTAGAACACTGCTTCAATC
CCCAATGAGGAGGATATGGC

Downstream 100 bases:

>100_bases
CCGACGGCGCCACCTTCACCATCCTGATCGTGGATGACGAGCAGCACATCCTGTTTTCTTCCGAATCCCTGCTCCGTTTT
GCCGGTTTCAGGGAGATACG

Product: PAS/PAC sensor signal transduction histidine kinase

Products: NA

Alternate protein names: Stage II sporulation protein F; Stage II sporulation protein J [H]

Number of amino acids: Translated: 653; Mature: 653

Protein sequence:

>653_residues
MDASRNSGCSSPKQLMHSILQAIPDLISVVDTDFKIVYSNWRGGHEYAAPNRRKQNRHCYEVYYPDLNGRCDPCHLVQVF
ETGMSIVAEKFNPRVGYLEIHAFPIFDASGTVVMAGEYLRNISDRKHAEDALRGANQMLEALVNASPPAILTLDLDLNLT
LWNPAAERMFGWKIDEVLGKSYPIVSIELMEELKENIRRLNRGEERQSMETRRLHRDGHFIDVSLSTAPMLNSEGGTIGY
VAIMADISERKQAQQALRESEANYRAIFDAANDATFVFDVETGAMLDVNLKMCQMYGYLREEVLLLNVEQLSAGFPPYTF
QDLMKLIWKTKRDQSHLFEWLAKDRSGRLFWVEVNMKGAVIGGEYKVLAVVRDISARKAAEEENRIMQERLLQANKMAAI
GTLASGIAHEINNPNNYILSNAQFLSDIWPEINRVLTRYAEENGEFFLGKLGYGEAAAMIPKMLTGLTEGAYRIKGIVTG
LKDFARQEKTRLDKPVDINKVIEAALSMLHNKIKQHTDNFSCSLDKALPPVRGRFRQLEQVIVNLTMNALESLPSRDRAV
SIQTSYSQYLEQITIRVEDQGIGMTEEVQKAIFDPFFTTKLDRGGTGLGLSINFTIVKEHNGIIECDSSPGKGTVFYVRI
PALRFEPEENGLP

Sequences:

>Translated_653_residues
MDASRNSGCSSPKQLMHSILQAIPDLISVVDTDFKIVYSNWRGGHEYAAPNRRKQNRHCYEVYYPDLNGRCDPCHLVQVF
ETGMSIVAEKFNPRVGYLEIHAFPIFDASGTVVMAGEYLRNISDRKHAEDALRGANQMLEALVNASPPAILTLDLDLNLT
LWNPAAERMFGWKIDEVLGKSYPIVSIELMEELKENIRRLNRGEERQSMETRRLHRDGHFIDVSLSTAPMLNSEGGTIGY
VAIMADISERKQAQQALRESEANYRAIFDAANDATFVFDVETGAMLDVNLKMCQMYGYLREEVLLLNVEQLSAGFPPYTF
QDLMKLIWKTKRDQSHLFEWLAKDRSGRLFWVEVNMKGAVIGGEYKVLAVVRDISARKAAEEENRIMQERLLQANKMAAI
GTLASGIAHEINNPNNYILSNAQFLSDIWPEINRVLTRYAEENGEFFLGKLGYGEAAAMIPKMLTGLTEGAYRIKGIVTG
LKDFARQEKTRLDKPVDINKVIEAALSMLHNKIKQHTDNFSCSLDKALPPVRGRFRQLEQVIVNLTMNALESLPSRDRAV
SIQTSYSQYLEQITIRVEDQGIGMTEEVQKAIFDPFFTTKLDRGGTGLGLSINFTIVKEHNGIIECDSSPGKGTVFYVRI
PALRFEPEENGLP
>Mature_653_residues
MDASRNSGCSSPKQLMHSILQAIPDLISVVDTDFKIVYSNWRGGHEYAAPNRRKQNRHCYEVYYPDLNGRCDPCHLVQVF
ETGMSIVAEKFNPRVGYLEIHAFPIFDASGTVVMAGEYLRNISDRKHAEDALRGANQMLEALVNASPPAILTLDLDLNLT
LWNPAAERMFGWKIDEVLGKSYPIVSIELMEELKENIRRLNRGEERQSMETRRLHRDGHFIDVSLSTAPMLNSEGGTIGY
VAIMADISERKQAQQALRESEANYRAIFDAANDATFVFDVETGAMLDVNLKMCQMYGYLREEVLLLNVEQLSAGFPPYTF
QDLMKLIWKTKRDQSHLFEWLAKDRSGRLFWVEVNMKGAVIGGEYKVLAVVRDISARKAAEEENRIMQERLLQANKMAAI
GTLASGIAHEINNPNNYILSNAQFLSDIWPEINRVLTRYAEENGEFFLGKLGYGEAAAMIPKMLTGLTEGAYRIKGIVTG
LKDFARQEKTRLDKPVDINKVIEAALSMLHNKIKQHTDNFSCSLDKALPPVRGRFRQLEQVIVNLTMNALESLPSRDRAV
SIQTSYSQYLEQITIRVEDQGIGMTEEVQKAIFDPFFTTKLDRGGTGLGLSINFTIVKEHNGIIECDSSPGKGTVFYVRI
PALRFEPEENGLP

Specific function: Phosphorylates the sporulation-regulatory proteins spo0A and spo0F. It also autophosphorylates in the presence of ATP [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=556, Percent_Identity=25.8992805755396, Blast_Score=120, Evalue=4e-28,
Organism=Escherichia coli, GI1790436, Length=257, Percent_Identity=27.6264591439689, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1790300, Length=271, Percent_Identity=28.7822878228782, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI87081816, Length=240, Percent_Identity=27.0833333333333, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI145693157, Length=302, Percent_Identity=24.5033112582781, Blast_Score=72, Evalue=2e-13,
Organism=Escherichia coli, GI1790346, Length=230, Percent_Identity=26.9565217391304, Blast_Score=69, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR013655
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS; PF08447 PAS_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 73665; Mature: 73665

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDASRNSGCSSPKQLMHSILQAIPDLISVVDTDFKIVYSNWRGGHEYAAPNRRKQNRHCY
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCEE
EVYYPDLNGRCDPCHLVQVFETGMSIVAEKFNPRVGYLEIHAFPIFDASGTVVMAGEYLR
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCEEECCHHHH
NISDRKHAEDALRGANQMLEALVNASPPAILTLDLDLNLTLWNPAAERMFGWKIDEVLGK
CCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEEECHHHHHHHCCCHHHHHCC
SYPIVSIELMEELKENIRRLNRGEERQSMETRRLHRDGHFIDVSLSTAPMLNSEGGTIGY
CCCEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEE
VAIMADISERKQAQQALRESEANYRAIFDAANDATFVFDVETGAMLDVNLKMCQMYGYLR
EEEEHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCEEECHHHHHHHHHHHH
EEVLLLNVEQLSAGFPPYTFQDLMKLIWKTKRDQSHLFEWLAKDRSGRLFWVEVNMKGAV
HHHHEEEHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEEEECCEEE
IGGEYKVLAVVRDISARKAAEEENRIMQERLLQANKMAAIGTLASGIAHEINNPNNYILS
ECCCEEEEHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
NAQFLSDIWPEINRVLTRYAEENGEFFLGKLGYGEAAAMIPKMLTGLTEGAYRIKGIVTG
CHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHH
LKDFARQEKTRLDKPVDINKVIEAALSMLHNKIKQHTDNFSCSLDKALPPVRGRFRQLEQ
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEHHHCCCHHHHHHHHHHH
VIVNLTMNALESLPSRDRAVSIQTSYSQYLEQITIRVEDQGIGMTEEVQKAIFDPFFTTK
HHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHEEEEEECCCCCCHHHHHHHHHCCHHHHE
LDRGGTGLGLSINFTIVKEHNGIIECDSSPGKGTVFYVRIPALRFEPEENGLP
ECCCCCEEEEEEEEEEEECCCCEEEECCCCCCCEEEEEEECCEEECCCCCCCC
>Mature Secondary Structure
MDASRNSGCSSPKQLMHSILQAIPDLISVVDTDFKIVYSNWRGGHEYAAPNRRKQNRHCY
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCEE
EVYYPDLNGRCDPCHLVQVFETGMSIVAEKFNPRVGYLEIHAFPIFDASGTVVMAGEYLR
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCEEECCHHHH
NISDRKHAEDALRGANQMLEALVNASPPAILTLDLDLNLTLWNPAAERMFGWKIDEVLGK
CCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEEECHHHHHHHCCCHHHHHCC
SYPIVSIELMEELKENIRRLNRGEERQSMETRRLHRDGHFIDVSLSTAPMLNSEGGTIGY
CCCEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEE
VAIMADISERKQAQQALRESEANYRAIFDAANDATFVFDVETGAMLDVNLKMCQMYGYLR
EEEEHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCEEECHHHHHHHHHHHH
EEVLLLNVEQLSAGFPPYTFQDLMKLIWKTKRDQSHLFEWLAKDRSGRLFWVEVNMKGAV
HHHHEEEHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEEEECCEEE
IGGEYKVLAVVRDISARKAAEEENRIMQERLLQANKMAAIGTLASGIAHEINNPNNYILS
ECCCEEEEHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
NAQFLSDIWPEINRVLTRYAEENGEFFLGKLGYGEAAAMIPKMLTGLTEGAYRIKGIVTG
CHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHH
LKDFARQEKTRLDKPVDINKVIEAALSMLHNKIKQHTDNFSCSLDKALPPVRGRFRQLEQ
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEHHHCCCHHHHHHHHHHH
VIVNLTMNALESLPSRDRAVSIQTSYSQYLEQITIRVEDQGIGMTEEVQKAIFDPFFTTK
HHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHEEEEEECCCCCCHHHHHHHHHCCHHHHE
LDRGGTGLGLSINFTIVKEHNGIIECDSSPGKGTVFYVRIPALRFEPEENGLP
ECCCCCEEEEEEEEEEEECCCCEEEECCCCCCCEEEEEEECCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2104615; 2509430; 9384377 [H]