Click here to switch to the map view.

The map label for this gene is nfsA [C]

Identifier: 118580413

GI number: 118580413

Start: 2144232

End: 2144780

Strand: Direct

Name: nfsA [C]

Synonym: Ppro_1997

Alternate gene names: 118580413

Gene position: 2144232-2144780 (Clockwise)

Preceding gene: 118580410

Following gene: 118580414

Centisome position: 53.5

GC content: 64.3

Gene sequence:

>549_bases
ATGATCGAACTGCTCCGCAAACGCCGCAGCATCCGCTGCTATACCGATGAACCCATAGACCGCGCAACCGTCGACCTGCT
GATCGAGGCGCTGCTGCGCGCGCCCTCCTCGCGAAACATCAACCCCTGGGAATTCATCGTGGTTGACGATCGCGACCAGC
TGGTACAACTCTCCCGGGCCAAGGAGCACGGCTCCGACTTTCTGAAACAGGCCCAGTTGGGCATCGTGGTCTGCGCCGAC
GCCAGGAAGAGCGACGTGTGGGTGGAGGACTGCTCCATCGCCTCCATACTCGTGCAGATGACCGCCCAGTCACGGGGGCT
GGGGAGCTGCTGGATCCAGATCCGCAAGCGTCCCCATGACACCACCGCCACGGCGGAAGAGTACATCCGGAAGATCCTGG
GCCTGCCGGAGCATCTCAGGGTCCTGTCCATGGTCAGCATCGGCCTTCCCGCCGAACAGCGCAAACCGCTGGGGGCGGAC
GAACTCCCCTGCCACAAGGTCAGGCACAACCGCTACGACGAGCCGTGGCAGCCGTCGGGCAGGCCCTGA

Upstream 100 bases:

>100_bases
CGGAAACGATTTCTCGTCAGGAGCCGCTTCCGAAGCGTTTTTCCGTTTCAGCTCCCCTCCGGTTTCCTGTATCATTGCGT
CATTTCAGGAGGTATGAACC

Downstream 100 bases:

>100_bases
CGGGGGAGCGGCAATGGCCGATTCCCCATTCGATTTCGACAGCATCGTCGACCGGAGCAACAGCGGCAGCGAGAAGTGGG
ACAGGTACAAGGGGCGCGAC

Product: nitroreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MIELLRKRRSIRCYTDEPIDRATVDLLIEALLRAPSSRNINPWEFIVVDDRDQLVQLSRAKEHGSDFLKQAQLGIVVCAD
ARKSDVWVEDCSIASILVQMTAQSRGLGSCWIQIRKRPHDTTATAEEYIRKILGLPEHLRVLSMVSIGLPAEQRKPLGAD
ELPCHKVRHNRYDEPWQPSGRP

Sequences:

>Translated_182_residues
MIELLRKRRSIRCYTDEPIDRATVDLLIEALLRAPSSRNINPWEFIVVDDRDQLVQLSRAKEHGSDFLKQAQLGIVVCAD
ARKSDVWVEDCSIASILVQMTAQSRGLGSCWIQIRKRPHDTTATAEEYIRKILGLPEHLRVLSMVSIGLPAEQRKPLGAD
ELPCHKVRHNRYDEPWQPSGRP
>Mature_182_residues
MIELLRKRRSIRCYTDEPIDRATVDLLIEALLRAPSSRNINPWEFIVVDDRDQLVQLSRAKEHGSDFLKQAQLGIVVCAD
ARKSDVWVEDCSIASILVQMTAQSRGLGSCWIQIRKRPHDTTATAEEYIRKILGLPEHLRVLSMVSIGLPAEQRKPLGAD
ELPCHKVRHNRYDEPWQPSGRP

Specific function: Reduction Of Nitroaromatic Compounds Using NADH. Reduces Nitrofurazone By A Ping-Pong Bi-Bi Mechanism Possibly To Generate A Two-Electron Transfer Product. Major Component Of The Oxygen- Insensitive Nitroreductase Activity In E.Coli. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 20787; Mature: 20787

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIELLRKRRSIRCYTDEPIDRATVDLLIEALLRAPSSRNINPWEFIVVDDRDQLVQLSRA
CHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHH
KEHGSDFLKQAQLGIVVCADARKSDVWVEDCSIASILVQMTAQSRGLGSCWIQIRKRPHD
HHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
TTATAEEYIRKILGLPEHLRVLSMVSIGLPAEQRKPLGADELPCHKVRHNRYDEPWQPSG
CHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHCCCCCCCCCCHHHHHHCCCCCCCCCCC
RP
CC
>Mature Secondary Structure
MIELLRKRRSIRCYTDEPIDRATVDLLIEALLRAPSSRNINPWEFIVVDDRDQLVQLSRA
CHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHH
KEHGSDFLKQAQLGIVVCADARKSDVWVEDCSIASILVQMTAQSRGLGSCWIQIRKRPHD
HHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
TTATAEEYIRKILGLPEHLRVLSMVSIGLPAEQRKPLGADELPCHKVRHNRYDEPWQPSG
CHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHCCCCCCCCCCHHHHHHCCCCCCCCCCC
RP
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]