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The map label for this gene is ghrA [H]

Identifier: 118580412

GI number: 118580412

Start: 2143114

End: 2144052

Strand: Reverse

Name: ghrA [H]

Synonym: Ppro_1996

Alternate gene names: 118580412

Gene position: 2144052-2143114 (Counterclockwise)

Preceding gene: 118580415

Following gene: 118580411

Centisome position: 53.49

GC content: 63.68

Gene sequence:

>939_bases
ATGCTACAGAAGAATCTCCTGGTACACCTGAAGAGCGATGTGGCCTCTTTCTCCATACAGGCGCGCCACCTGGAGCGCAT
ACGCCAGGCCTTTCCCGGTGTCAACCTGCTGGAGGCCGGCGGGAGGGATGAACTGCGCCATCTGCTGGGCGAGGCGGACT
GGCTCTTGACCTGGCAGTTCAGGGCCGACCGCTATGAACTGCTGCCCCGACTGGAGGCGGTATTCACCCCGGCAGCCGGG
AGAGACTGGGTCCCCGCCGATCCATCCGGCAGGGTCAGGAACTTTTACGGCAGGTTCCACGGCCGCATCATGCGCGAGAG
CCTGCTGGCCATGATGCTCTATTTCAACCGCCGCCTGGGGCACTGCCGTGAGAACCAGAATAACCGGCTCTGGGACCGCA
ACGCCCTGGATGGCAGCTCTTCGCTCTTCAGCCAGCAGATACTGATCATAGGCTACGGCGCCATCGGCCGCCAGATGGCG
GAGCTGATCCGGGCCTTTGGTGCACGCATCACCGGGGTCAAGCGCAGCAGGGAGGGATTCGAGAACGATCCCTTTGCCCA
GCGGGTGGTGACCTTCGACAGCCTGGAAGATGAGCTCCCCCTGGCGGACCACGTGGTGCTGATGCTCCCCGGCGGCCCCC
GGACCGAGGATCTGTTCACCCAGCGTCATTTCCAGTTGATGAAACCGGGCTCGTATCTGTACAATCTCGGCCGGGGCAGC
TGCTATCGCGAGGAGCATCTGATCAGCGCCCTGCGGGACGGCCCCCTGGCGGGAGCCGGGTTGGACGTGTTCCGCGAGGA
ACCGCTCCCCCCTTCCTCGCCGCTCTGGAGGCTTCCCAATGTGCTGATCACGCCCCACGCCAGCGCCATCAGCCGCGAGT
ATCTGGATCTGTACCTGGAGGAGTGGATCGGCATTGTCTGGGAAACCTGGGGAGAATGA

Upstream 100 bases:

>100_bases
CCTGACGAGAAATCGTTTCCGGCTCGCGCAACTATTTGGTTGATGCCCCGAATCTCAGGATGGTATACCTACGCAGAAAT
CGGCTTCCATGGGGGTACTC

Downstream 100 bases:

>100_bases
TCCGGGGCGAACGCAGTACGGCTCCACCTGCCGCATCCAGGTTCGAAACCGTTTTTTTTATATCACACGAGAGAGGTAGT
GATCACCAATGACACAGAAT

Product: NAD-binding D-isomer specific 2-hydroxyacid dehydrogenase

Products: NA

Alternate protein names: 2-ketoacid reductase [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MLQKNLLVHLKSDVASFSIQARHLERIRQAFPGVNLLEAGGRDELRHLLGEADWLLTWQFRADRYELLPRLEAVFTPAAG
RDWVPADPSGRVRNFYGRFHGRIMRESLLAMMLYFNRRLGHCRENQNNRLWDRNALDGSSSLFSQQILIIGYGAIGRQMA
ELIRAFGARITGVKRSREGFENDPFAQRVVTFDSLEDELPLADHVVLMLPGGPRTEDLFTQRHFQLMKPGSYLYNLGRGS
CYREEHLISALRDGPLAGAGLDVFREEPLPPSSPLWRLPNVLITPHASAISREYLDLYLEEWIGIVWETWGE

Sequences:

>Translated_312_residues
MLQKNLLVHLKSDVASFSIQARHLERIRQAFPGVNLLEAGGRDELRHLLGEADWLLTWQFRADRYELLPRLEAVFTPAAG
RDWVPADPSGRVRNFYGRFHGRIMRESLLAMMLYFNRRLGHCRENQNNRLWDRNALDGSSSLFSQQILIIGYGAIGRQMA
ELIRAFGARITGVKRSREGFENDPFAQRVVTFDSLEDELPLADHVVLMLPGGPRTEDLFTQRHFQLMKPGSYLYNLGRGS
CYREEHLISALRDGPLAGAGLDVFREEPLPPSSPLWRLPNVLITPHASAISREYLDLYLEEWIGIVWETWGE
>Mature_312_residues
MLQKNLLVHLKSDVASFSIQARHLERIRQAFPGVNLLEAGGRDELRHLLGEADWLLTWQFRADRYELLPRLEAVFTPAAG
RDWVPADPSGRVRNFYGRFHGRIMRESLLAMMLYFNRRLGHCRENQNNRLWDRNALDGSSSLFSQQILIIGYGAIGRQMA
ELIRAFGARITGVKRSREGFENDPFAQRVVTFDSLEDELPLADHVVLMLPGGPRTEDLFTQRHFQLMKPGSYLYNLGRGS
CYREEHLISALRDGPLAGAGLDVFREEPLPPSSPLWRLPNVLITPHASAISREYLDLYLEEWIGIVWETWGE

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=202, Percent_Identity=29.7029702970297, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI6912396, Length=184, Percent_Identity=31.5217391304348, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI61743967, Length=158, Percent_Identity=32.2784810126582, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI4557497, Length=158, Percent_Identity=32.2784810126582, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI145580578, Length=158, Percent_Identity=30.379746835443, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI4557499, Length=158, Percent_Identity=30.379746835443, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI87081824, Length=282, Percent_Identity=30.4964539007092, Blast_Score=104, Evalue=9e-24,
Organism=Escherichia coli, GI87082289, Length=255, Percent_Identity=29.0196078431373, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1789279, Length=168, Percent_Identity=34.5238095238095, Blast_Score=86, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17532191, Length=282, Percent_Identity=24.822695035461, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320925, Length=206, Percent_Identity=29.126213592233, Blast_Score=80, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6322116, Length=157, Percent_Identity=33.1210191082803, Blast_Score=79, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6325144, Length=197, Percent_Identity=26.9035532994924, Blast_Score=76, Evalue=6e-15,
Organism=Drosophila melanogaster, GI28571528, Length=257, Percent_Identity=29.9610894941634, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI45552429, Length=239, Percent_Identity=28.0334728033473, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24585514, Length=238, Percent_Identity=27.3109243697479, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI28574282, Length=238, Percent_Identity=27.3109243697479, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI28574284, Length=239, Percent_Identity=28.0334728033473, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI45551003, Length=239, Percent_Identity=28.0334728033473, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19921140, Length=167, Percent_Identity=31.1377245508982, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24585516, Length=137, Percent_Identity=35.036496350365, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI28574286, Length=189, Percent_Identity=28.042328042328, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 35782; Mature: 35782

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQKNLLVHLKSDVASFSIQARHLERIRQAFPGVNLLEAGGRDELRHLLGEADWLLTWQF
CCCCCHHEEEHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHCCCCEEEEEEE
RADRYELLPRLEAVFTPAAGRDWVPADPSGRVRNFYGRFHGRIMRESLLAMMLYFNRRLG
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HCRENQNNRLWDRNALDGSSSLFSQQILIIGYGAIGRQMAELIRAFGARITGVKRSREGF
HHHCCCCCCCCCCCCCCCCHHHHHCEEEEEECCHHHHHHHHHHHHHHHHHHCCHHHHCCC
ENDPFAQRVVTFDSLEDELPLADHVVLMLPGGPRTEDLFTQRHFQLMKPGSYLYNLGRGS
CCCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCHHHHCCCCCC
CYREEHLISALRDGPLAGAGLDVFREEPLPPSSPLWRLPNVLITPHASAISREYLDLYLE
HHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCHHHCCCEEECCCHHHHHHHHHHHHHH
EWIGIVWETWGE
HHHHHHHHCCCC
>Mature Secondary Structure
MLQKNLLVHLKSDVASFSIQARHLERIRQAFPGVNLLEAGGRDELRHLLGEADWLLTWQF
CCCCCHHEEEHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHCCCCEEEEEEE
RADRYELLPRLEAVFTPAAGRDWVPADPSGRVRNFYGRFHGRIMRESLLAMMLYFNRRLG
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HCRENQNNRLWDRNALDGSSSLFSQQILIIGYGAIGRQMAELIRAFGARITGVKRSREGF
HHHCCCCCCCCCCCCCCCCHHHHHCEEEEEECCHHHHHHHHHHHHHHHHHHCCHHHHCCC
ENDPFAQRVVTFDSLEDELPLADHVVLMLPGGPRTEDLFTQRHFQLMKPGSYLYNLGRGS
CCCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCHHHHCCCCCC
CYREEHLISALRDGPLAGAGLDVFREEPLPPSSPLWRLPNVLITPHASAISREYLDLYLE
HHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCHHHCCCEEECCCHHHHHHHHHHHHHH
EWIGIVWETWGE
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]