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The map label for this gene is cphA [H]

Identifier: 118580405

GI number: 118580405

Start: 2131088

End: 2132371

Strand: Direct

Name: cphA [H]

Synonym: Ppro_1989

Alternate gene names: 118580405

Gene position: 2131088-2132371 (Clockwise)

Preceding gene: 118580404

Following gene: 118580406

Centisome position: 53.17

GC content: 54.05

Gene sequence:

>1284_bases
ATGGCCAATCACCCCGCCTCCAAAAACACTGCTCGACATATGTACAGAAAATGGGTTCTGTTTGTGCGCAAACTGGGCTT
CCCGCTGAAGATCACGCCACATATCCCAACCGCTTGCATAACGGGCAGTGTGGGAAAAACAACGACGTGCCGAATGGTTG
CATCTATTTTGCGGGAAACGGGCAAAACGGTCGCACTGAGCACGACTCTCGGTACATATGTGGGAGACAGTCCTCGGTGT
ATCGGCGATTCTTCAAATGGCCGCCGCGGCAGTCGGTTGTTGGCAGAGCGCCAAGTTGACGCAGGGGTGTTTGAAATTGC
ACGAGGCGGTCTCATTAATGAGGGAATGTTGTTTTCCAACTACGATATCGGCGCGCTGCTGAATGTTTACGACAACCACA
TCGGCCTGAAGGGGGTTGAGTCTCGGGCCGATCTGGCCAAAGTAAAAAAACAGGTGGTGGTCAATGCCCGGAAAATGGCC
ATTTTGAACGCGGACGACCCTTTGTGTCTTGGGGTAAAGAAGGACATTCGTGCCGCGCGCACATGTCTGATCACGATGAC
TGCTGAGAGCCCCGTTGTGCGCGATCACATGTCCAGGGGGGGCGTAGCTGTTCGTCAGGAAGGCAACGGGGCAGAGGCGA
TGATCAGGTTGTACGAGGGCAACAAAATGGTCCTCTCCATGGCAGCTCTGGATATTCCTGCCAGTTGGGGAGGTCGATAT
CGGCCAGCAATCAACAATGCCATGTTTGCTGCAGCCATTACGTATGGGCTGGATGTTTCTACACAATCCATACGAGCGGG
CTTGCGTAGCTTTCAGTCAGATGAGGTCAGCAACCCAGGCAGAATGAATTTTTATGACCATCTACCCTGCGGTTTGCTGA
TTACCTGGGCAGACGGCCCGGAGGCGGCCTGCGAAATTGCCGATTTTGTGTCCCTCCATACCATTTCAGGGAAAAAGCAT
CTGATGCTCGCGGCTATGGGAAATCGCCCGGATGCTTTCATCGTGGAGACGGCGCTGGCGTTTTCGGGCAGATTTGACAG
CTACATCAGCACCGACTGGGAAGACTTGCGTGGGCGTGCGCCTGGGGAAGTTGCCGGGCTTCTGGCCAGCGGGTTCAAAA
ATGGCGGGGTCGATCAAAGCAGGATAGAAATCGCCACGTCGTATGATGATGGCTTGTCACGTGCATTTGCCAAGCCGTCT
CAGGGGGACTTGCTCGTCATTGTGAGTTTCAGCGGCTGGAGGGCAAAAGAACAGGGCTTGTTCAAAAGCAAAAACATCCC
ATGA

Upstream 100 bases:

>100_bases
GACCTGCGTAATTGCTTTACCAAATGCGGAGCTCTGACCCCTCAGCATGCTTACTCCGCGCTTACAGCAAATATATTACA
AAACCCCAGAGGGTCCCCTC

Downstream 100 bases:

>100_bases
ACGAGACATCGCACAAGGAGGCAGAAGCCATGGGAAGATCAGCTTCAGGTGCCGTGACGGCACTTATCACGATTGCCCTG
CTCGCGGCTTTCGGCTGCGA

Product: Mur ligase middle domain-containing protein

Products: NA

Alternate protein names: Cyanophycin synthase [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MANHPASKNTARHMYRKWVLFVRKLGFPLKITPHIPTACITGSVGKTTTCRMVASILRETGKTVALSTTLGTYVGDSPRC
IGDSSNGRRGSRLLAERQVDAGVFEIARGGLINEGMLFSNYDIGALLNVYDNHIGLKGVESRADLAKVKKQVVVNARKMA
ILNADDPLCLGVKKDIRAARTCLITMTAESPVVRDHMSRGGVAVRQEGNGAEAMIRLYEGNKMVLSMAALDIPASWGGRY
RPAINNAMFAAAITYGLDVSTQSIRAGLRSFQSDEVSNPGRMNFYDHLPCGLLITWADGPEAACEIADFVSLHTISGKKH
LMLAAMGNRPDAFIVETALAFSGRFDSYISTDWEDLRGRAPGEVAGLLASGFKNGGVDQSRIEIATSYDDGLSRAFAKPS
QGDLLVIVSFSGWRAKEQGLFKSKNIP

Sequences:

>Translated_427_residues
MANHPASKNTARHMYRKWVLFVRKLGFPLKITPHIPTACITGSVGKTTTCRMVASILRETGKTVALSTTLGTYVGDSPRC
IGDSSNGRRGSRLLAERQVDAGVFEIARGGLINEGMLFSNYDIGALLNVYDNHIGLKGVESRADLAKVKKQVVVNARKMA
ILNADDPLCLGVKKDIRAARTCLITMTAESPVVRDHMSRGGVAVRQEGNGAEAMIRLYEGNKMVLSMAALDIPASWGGRY
RPAINNAMFAAAITYGLDVSTQSIRAGLRSFQSDEVSNPGRMNFYDHLPCGLLITWADGPEAACEIADFVSLHTISGKKH
LMLAAMGNRPDAFIVETALAFSGRFDSYISTDWEDLRGRAPGEVAGLLASGFKNGGVDQSRIEIATSYDDGLSRAFAKPS
QGDLLVIVSFSGWRAKEQGLFKSKNIP
>Mature_426_residues
ANHPASKNTARHMYRKWVLFVRKLGFPLKITPHIPTACITGSVGKTTTCRMVASILRETGKTVALSTTLGTYVGDSPRCI
GDSSNGRRGSRLLAERQVDAGVFEIARGGLINEGMLFSNYDIGALLNVYDNHIGLKGVESRADLAKVKKQVVVNARKMAI
LNADDPLCLGVKKDIRAARTCLITMTAESPVVRDHMSRGGVAVRQEGNGAEAMIRLYEGNKMVLSMAALDIPASWGGRYR
PAINNAMFAAAITYGLDVSTQSIRAGLRSFQSDEVSNPGRMNFYDHLPCGLLITWADGPEAACEIADFVSLHTISGKKHL
MLAAMGNRPDAFIVETALAFSGRFDSYISTDWEDLRGRAPGEVAGLLASGFKNGGVDQSRIEIATSYDDGLSRAFAKPSQ
GDLLVIVSFSGWRAKEQGLFKSKNIP

Specific function: Catalyzes the ATP-dependent polymerization of arginine and aspartate to multi-L-arginyl-poly-L-aspartic acid (cyanophycin; a water-insoluble reserve polymer) [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011810
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; PF08443 RimK [H]

EC number: =6.3.2.29; =6.3.2.30 [H]

Molecular weight: Translated: 46150; Mature: 46018

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANHPASKNTARHMYRKWVLFVRKLGFPLKITPHIPTACITGSVGKTTTCRMVASILRET
CCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCEEECCCCCCHHHHHHHHHHHHC
GKTVALSTTLGTYVGDSPRCIGDSSNGRRGSRLLAERQVDAGVFEIARGGLINEGMLFSN
CCEEEEEEEHHHHCCCCCCEECCCCCCCCHHHHHHHHHHCCHHHHHHCCCCCCCCEEEEC
YDIGALLNVYDNHIGLKGVESRADLAKVKKQVVVNARKMAILNADDPLCLGVKKDIRAAR
CCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCEEECCHHHHHHHE
TCLITMTAESPVVRDHMSRGGVAVRQEGNGAEAMIRLYEGNKMVLSMAALDIPASWGGRY
EEEEEEECCCCHHHHHHCCCCEEEEECCCCCEEEEEEECCCEEEEEEEEECCCCCCCCCC
RPAINNAMFAAAITYGLDVSTQSIRAGLRSFQSDEVSNPGRMNFYDHLPCGLLITWADGP
CCHHCCHHEEHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCC
EAACEIADFVSLHTISGKKHLMLAAMGNRPDAFIVETALAFSGRFDSYISTDWEDLRGRA
HHHHHHHHHHEEEEECCCCEEEEEEECCCCCEEEEEHHHHHCCCCCCHHCCCHHHHCCCC
PGEVAGLLASGFKNGGVDQSRIEIATSYDDGLSRAFAKPSQGDLLVIVSFSGWRAKEQGL
CHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHCCCCCCCEEEEEECCCCCCHHCCC
FKSKNIP
CCCCCCC
>Mature Secondary Structure 
ANHPASKNTARHMYRKWVLFVRKLGFPLKITPHIPTACITGSVGKTTTCRMVASILRET
CCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCEEECCCCCCHHHHHHHHHHHHC
GKTVALSTTLGTYVGDSPRCIGDSSNGRRGSRLLAERQVDAGVFEIARGGLINEGMLFSN
CCEEEEEEEHHHHCCCCCCEECCCCCCCCHHHHHHHHHHCCHHHHHHCCCCCCCCEEEEC
YDIGALLNVYDNHIGLKGVESRADLAKVKKQVVVNARKMAILNADDPLCLGVKKDIRAAR
CCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCEEECCHHHHHHHE
TCLITMTAESPVVRDHMSRGGVAVRQEGNGAEAMIRLYEGNKMVLSMAALDIPASWGGRY
EEEEEEECCCCHHHHHHCCCCEEEEECCCCCEEEEEEECCCEEEEEEEEECCCCCCCCCC
RPAINNAMFAAAITYGLDVSTQSIRAGLRSFQSDEVSNPGRMNFYDHLPCGLLITWADGP
CCHHCCHHEEHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCC
EAACEIADFVSLHTISGKKHLMLAAMGNRPDAFIVETALAFSGRFDSYISTDWEDLRGRA
HHHHHHHHHHEEEEECCCCEEEEEEECCCCCEEEEEHHHHHCCCCCCHHCCCHHHHCCCC
PGEVAGLLASGFKNGGVDQSRIEIATSYDDGLSRAFAKPSQGDLLVIVSFSGWRAKEQGL
CHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHCCCCCCCEEEEEECCCCCCHHCCC
FKSKNIP
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]