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The map label for this gene is macA [H]

Identifier: 118580399

GI number: 118580399

Start: 2125699

End: 2126949

Strand: Direct

Name: macA [H]

Synonym: Ppro_1983

Alternate gene names: 118580399

Gene position: 2125699-2126949 (Clockwise)

Preceding gene: 118580391

Following gene: 118580400

Centisome position: 53.04

GC content: 66.59

Gene sequence:

>1251_bases
ATGCTGACGCCCCCTGGGGAGAACGGGTATTCCATGGAACACGAAGACCTGGACCAGTTGAAAATCGACAAGACGGTCAC
TGCGCCCCGCTCCGGAACTTCCCGGGGGAGGCGCTTCTGGGCAGCGGGGATTCTGCTGGTCGTCCTGGCGGTCTCCCTCT
ACGTCAGTGGTGTGCTGCGCCCCGCTGAAACCGTGGAGGTCGTGACCGTACAACAGATTTTCCCTTCCCAGGGCTTTACC
CTGCTCAGCGCCAGCGGCTACGTCGTAGCCCAGCGCAAGGCCGCGGTGGCTTCCAAGGCGACCGGTCGCCTGGTCTGGCT
GGGGGTCGAGGAGGGGAGTGCCGTCCGCCAGGGGGACGTGATTGCCCGGATCGAAAACGCCGACGTGCTGGCCATCCAGT
CCCAGGCGGCGGCGAACCTGAAGAACTGTCGCGCGGATCTGGACCAGGCCCTGGCGGAACTGCACGACGCCCACCTTTCC
TTCAAGCGCTACACGATCCTGCTCTCCGATGGGGTCGTCTCCAGGGCTGACTTCGATGCGGCCCAGGCTCGCTACCGCAA
GGCACAGGCCGCGGTCGCCGGCGCCAGGGCCGCCATCCGGTCGGCCTCGGCCGCTCTGGATGGCGCCAGGGTAGCGGTGG
AGTTCACCTCCATCAGGGCTCCCTTCGACGCAGTGGTGCTGACCAAGAACGCCGATGTGGGGGATATCGTCACCCCCCTT
GCCTCGGCGGCCAACGCCAAGGCGGCGGTGGTTACCATCGCCGACATGGGCTCCCTGCAGGTCGAGGTCGATGTCATGGA
ATCCAGCCTTGAGAAGGTCCGGGTGGGGCAACCGTGCGAGATCCAGCTGGACTCCTTCCCCGACCTGCGCTTTCGCGGAG
CTGTCCACATGGTCGTGCCCACGGCGGACCGGAGCAAGGCCACCGTGCTGGTCAAGGTCAGGTTCCTGGACTTCGACCGG
CGGATCCTGCCGGAGATGAGCGCCAGGGTGGCCTTCCTCTCCCGGCCGGTGGCAAACAGCGAACAGCGGCCGCGCACAAC
CATCCCCAGCGCTGCGCTGGTCCGGGGCCGCGGGCGAAACCTGGTCTTCCTGGTGGAGGGCGACCGGATCAAGGAGAAAC
CGGTTAACCTGGGGGTGAAGGTGGGTGACCTGGTGGAGGTGACCAGTGGCGTAAAGAGCGGGGACAGCCTGGTCCTGCGC
CCCGCCGGCAGCCTGCGGGACGGTTCCCGCATCAGCATTCCCGAGAAATGA

Upstream 100 bases:

>100_bases
TCACGGCGTCTACGCCGGGAGTGGAAGCCATCCCCTTTGACGTGGCGCATATGGCGGTATATAGTTCCTGGCATTATAAC
GGTTTAGGATCCGCGCAGGC

Downstream 100 bases:

>100_bases
ACCAGACAAAACCCGACATCGTCCAGATACGGAACCTCTCCAAATCCTATCGCAGGGGGAGCCAGATCATCCCGGTGCTG
GAGGATATCAGCTTCCATAT

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MLTPPGENGYSMEHEDLDQLKIDKTVTAPRSGTSRGRRFWAAGILLVVLAVSLYVSGVLRPAETVEVVTVQQIFPSQGFT
LLSASGYVVAQRKAAVASKATGRLVWLGVEEGSAVRQGDVIARIENADVLAIQSQAAANLKNCRADLDQALAELHDAHLS
FKRYTILLSDGVVSRADFDAAQARYRKAQAAVAGARAAIRSASAALDGARVAVEFTSIRAPFDAVVLTKNADVGDIVTPL
ASAANAKAAVVTIADMGSLQVEVDVMESSLEKVRVGQPCEIQLDSFPDLRFRGAVHMVVPTADRSKATVLVKVRFLDFDR
RILPEMSARVAFLSRPVANSEQRPRTTIPSAALVRGRGRNLVFLVEGDRIKEKPVNLGVKVGDLVEVTSGVKSGDSLVLR
PAGSLRDGSRISIPEK

Sequences:

>Translated_416_residues
MLTPPGENGYSMEHEDLDQLKIDKTVTAPRSGTSRGRRFWAAGILLVVLAVSLYVSGVLRPAETVEVVTVQQIFPSQGFT
LLSASGYVVAQRKAAVASKATGRLVWLGVEEGSAVRQGDVIARIENADVLAIQSQAAANLKNCRADLDQALAELHDAHLS
FKRYTILLSDGVVSRADFDAAQARYRKAQAAVAGARAAIRSASAALDGARVAVEFTSIRAPFDAVVLTKNADVGDIVTPL
ASAANAKAAVVTIADMGSLQVEVDVMESSLEKVRVGQPCEIQLDSFPDLRFRGAVHMVVPTADRSKATVLVKVRFLDFDR
RILPEMSARVAFLSRPVANSEQRPRTTIPSAALVRGRGRNLVFLVEGDRIKEKPVNLGVKVGDLVEVTSGVKSGDSLVLR
PAGSLRDGSRISIPEK
>Mature_416_residues
MLTPPGENGYSMEHEDLDQLKIDKTVTAPRSGTSRGRRFWAAGILLVVLAVSLYVSGVLRPAETVEVVTVQQIFPSQGFT
LLSASGYVVAQRKAAVASKATGRLVWLGVEEGSAVRQGDVIARIENADVLAIQSQAAANLKNCRADLDQALAELHDAHLS
FKRYTILLSDGVVSRADFDAAQARYRKAQAAVAGARAAIRSASAALDGARVAVEFTSIRAPFDAVVLTKNADVGDIVTPL
ASAANAKAAVVTIADMGSLQVEVDVMESSLEKVRVGQPCEIQLDSFPDLRFRGAVHMVVPTADRSKATVLVKVRFLDFDR
RILPEMSARVAFLSRPVANSEQRPRTTIPSAALVRGRGRNLVFLVEGDRIKEKPVNLGVKVGDLVEVTSGVKSGDSLVLR
PAGSLRDGSRISIPEK

Specific function: Efflux transporter for macrolide antibiotics [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=320, Percent_Identity=27.1875, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI87082036, Length=368, Percent_Identity=25, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789929, Length=337, Percent_Identity=21.6617210682493, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1790519, Length=241, Percent_Identity=25.7261410788382, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789665, Length=335, Percent_Identity=23.5820895522388, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 44473; Mature: 44473

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTPPGENGYSMEHEDLDQLKIDKTVTAPRSGTSRGRRFWAAGILLVVLAVSLYVSGVLR
CCCCCCCCCCCCCCCCHHHEEECCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
PAETVEVVTVQQIFPSQGFTLLSASGYVVAQRKAAVASKATGRLVWLGVEEGSAVRQGDV
CCHHEEEEEEEHHCCCCCEEEEECCCEEEEECHHHHHHCCCCCEEEEEECCCCCEECCCE
IARIENADVLAIQSQAAANLKNCRADLDQALAELHDAHLSFKRYTILLSDGVVSRADFDA
EEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCCHH
AQARYRKAQAAVAGARAAIRSASAALDGARVAVEFTSIRAPFDAVVLTKNADVGDIVTPL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEEECCCCCCHHHHHH
ASAANAKAAVVTIADMGSLQVEVDVMESSLEKVRVGQPCEIQLDSFPDLRFRGAVHMVVP
HHCCCCCEEEEEEECCCCEEEEEEHHHHHHHHHCCCCCCEEEECCCCCEEECCEEEEEEE
TADRSKATVLVKVRFLDFDRRILPEMSARVAFLSRPVANSEQRPRTTIPSAALVRGRGRN
CCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCC
LVFLVEGDRIKEKPVNLGVKVGDLVEVTSGVKSGDSLVLRPAGSLRDGSRISIPEK
EEEEEECCCCCCCCCCCCCEECHHHHHHCCCCCCCCEEEECCCCCCCCCEECCCCC
>Mature Secondary Structure
MLTPPGENGYSMEHEDLDQLKIDKTVTAPRSGTSRGRRFWAAGILLVVLAVSLYVSGVLR
CCCCCCCCCCCCCCCCHHHEEECCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
PAETVEVVTVQQIFPSQGFTLLSASGYVVAQRKAAVASKATGRLVWLGVEEGSAVRQGDV
CCHHEEEEEEEHHCCCCCEEEEECCCEEEEECHHHHHHCCCCCEEEEEECCCCCEECCCE
IARIENADVLAIQSQAAANLKNCRADLDQALAELHDAHLSFKRYTILLSDGVVSRADFDA
EEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCCHH
AQARYRKAQAAVAGARAAIRSASAALDGARVAVEFTSIRAPFDAVVLTKNADVGDIVTPL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEEECCCCCCHHHHHH
ASAANAKAAVVTIADMGSLQVEVDVMESSLEKVRVGQPCEIQLDSFPDLRFRGAVHMVVP
HHCCCCCEEEEEEECCCCEEEEEEHHHHHHHHHCCCCCCEEEECCCCCEEECCEEEEEEE
TADRSKATVLVKVRFLDFDRRILPEMSARVAFLSRPVANSEQRPRTTIPSAALVRGRGRN
CCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCC
LVFLVEGDRIKEKPVNLGVKVGDLVEVTSGVKSGDSLVLRPAGSLRDGSRISIPEK
EEEEEECCCCCCCCCCCCCEECHHHHHHCCCCCCCCEEEECCCCCCCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]