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The map label for this gene is leuC [H]

Identifier: 118580343

GI number: 118580343

Start: 2075802

End: 2077085

Strand: Direct

Name: leuC [H]

Synonym: Ppro_1926

Alternate gene names: 118580343

Gene position: 2075802-2077085 (Clockwise)

Preceding gene: 118580342

Following gene: 118580344

Centisome position: 51.79

GC content: 62.85

Gene sequence:

>1284_bases
ATGCCCAAGACCATCGCGGAGAAGATATTCGAGAGTCACCTGGTTGACGAGCCCTTTCCCGGCACAAAGGTCCTGCGCCT
GGACGTCGTGCTCTGCCATGAGATCACCACCCCCATCGCCATCGACGACCTGGTGCGCCGTAACAAGGACCGGGTCTTCG
ACAGCACCAAGATCAAGGCGGTCATCGACCACGTCACCCCCAGCAAGGACAGTAAAACTGCCACCCAGGCAAAGATCCTG
CGCGACTGGGCCCGGCGCCACGACATCCCGGACTTCTTTGACATAGGCGCAAACGGCGTCTGCCACGCCCTGTTCCCGGA
GAAAGGGTTCATCCGCCCCGGCTACACCGTGATCATGGGAGACTCCCACACCTGCACCCACGGCGCCTTCGGCGCCTTTG
CCGCCGGCATCGGCACCACCGACCTGGAGGTGGGCATCCTGAAGGGGGTCTGCGCCTTCCGCCAGCCAAAGACCATCCGC
TTCAACGTCATTGGCCAGCTACCCACAGGGGTCTATGCCAAGGACGTGATCCTGCACATCATAGGCAGAATCGGCGTCAA
CGGCGCCACCGACCGGGTGATGGAGTTCCACGGCGACACCATCGCCGCCATGACCATGGATTCCCGCATGGCCCTGTGCA
ACATGGCCATCGAGGCTGGCGGCACCTCCGGCATCTGCATGCCGGACATGACCACCGTTGACTACCTGTGGCCCTTCATA
AGCGACGAGTTCGAGAGCAAGGAAGCCGCCCTGGCCGAGTACGGTAAATGGTGCTCCGACCCGGACGCCTCGTACGAGCA
AGTGATCGACATCGACGTCACCAACCTGGAGCCGACGGTCACCTTCGGCTACAAGCCTGACCAGGTCAAGCCGGTGACCG
AGCTGGCCGGAACAAAAGTGGACCAGATCTACCTGGGCTCCTGTACCAACGGCCGGCTGGAGGACCTGCGCATCGCCGCA
GGCATCCTGAAGGGGAAAAAGCTCGCCCCCCATGTACGCGGCATCCTCTCCCCGGCAACCCCCAAGGTGTACCAGGAGGC
GCTGAAGGAAGGGCTGATGGAGATCTTCATGGAGGCCGGTTTCTGCATCACCAACCCCACCTGTGGCGCCTGCCTGGGCA
TGTCCAACGGTGTCTTGGCCGAAGGGGAGGTCTGCGCCGCCACCACCAACCGCAACTTCATGGGCAGGATGGGCAAGGGA
GGCATGGTGCACCTGATGTCGCCGGCCACCGCTGCAGCCAGCGCCATCGAAGGGGTCATCGCCGACCCGAGAAAGTACCT
GTAA

Upstream 100 bases:

>100_bases
GCCCTCTCCATACGCGATGGCAGCCTCGCCAATCGCCGCTTCCCCTGGCCATTGCTTTCGCGTATGGTTGACTGCATCCC
ACATCCAAAGAGGTACAGCC

Downstream 100 bases:

>100_bases
TCGGTGACCATCCCGTAGGGGCGGTCCCATGTGGCCGCCCTTGCCCCCTCCCCCTGCTAACAAATACAGCAGGGCACCCA
CACGGGGGTGCCCCTACGCC

Product: 3-isopropylmalate dehydratase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MPKTIAEKIFESHLVDEPFPGTKVLRLDVVLCHEITTPIAIDDLVRRNKDRVFDSTKIKAVIDHVTPSKDSKTATQAKIL
RDWARRHDIPDFFDIGANGVCHALFPEKGFIRPGYTVIMGDSHTCTHGAFGAFAAGIGTTDLEVGILKGVCAFRQPKTIR
FNVIGQLPTGVYAKDVILHIIGRIGVNGATDRVMEFHGDTIAAMTMDSRMALCNMAIEAGGTSGICMPDMTTVDYLWPFI
SDEFESKEAALAEYGKWCSDPDASYEQVIDIDVTNLEPTVTFGYKPDQVKPVTELAGTKVDQIYLGSCTNGRLEDLRIAA
GILKGKKLAPHVRGILSPATPKVYQEALKEGLMEIFMEAGFCITNPTCGACLGMSNGVLAEGEVCAATTNRNFMGRMGKG
GMVHLMSPATAAASAIEGVIADPRKYL

Sequences:

>Translated_427_residues
MPKTIAEKIFESHLVDEPFPGTKVLRLDVVLCHEITTPIAIDDLVRRNKDRVFDSTKIKAVIDHVTPSKDSKTATQAKIL
RDWARRHDIPDFFDIGANGVCHALFPEKGFIRPGYTVIMGDSHTCTHGAFGAFAAGIGTTDLEVGILKGVCAFRQPKTIR
FNVIGQLPTGVYAKDVILHIIGRIGVNGATDRVMEFHGDTIAAMTMDSRMALCNMAIEAGGTSGICMPDMTTVDYLWPFI
SDEFESKEAALAEYGKWCSDPDASYEQVIDIDVTNLEPTVTFGYKPDQVKPVTELAGTKVDQIYLGSCTNGRLEDLRIAA
GILKGKKLAPHVRGILSPATPKVYQEALKEGLMEIFMEAGFCITNPTCGACLGMSNGVLAEGEVCAATTNRNFMGRMGKG
GMVHLMSPATAAASAIEGVIADPRKYL
>Mature_426_residues
PKTIAEKIFESHLVDEPFPGTKVLRLDVVLCHEITTPIAIDDLVRRNKDRVFDSTKIKAVIDHVTPSKDSKTATQAKILR
DWARRHDIPDFFDIGANGVCHALFPEKGFIRPGYTVIMGDSHTCTHGAFGAFAAGIGTTDLEVGILKGVCAFRQPKTIRF
NVIGQLPTGVYAKDVILHIIGRIGVNGATDRVMEFHGDTIAAMTMDSRMALCNMAIEAGGTSGICMPDMTTVDYLWPFIS
DEFESKEAALAEYGKWCSDPDASYEQVIDIDVTNLEPTVTFGYKPDQVKPVTELAGTKVDQIYLGSCTNGRLEDLRIAAG
ILKGKKLAPHVRGILSPATPKVYQEALKEGLMEIFMEAGFCITNPTCGACLGMSNGVLAEGEVCAATTNRNFMGRMGKGG
MVHLMSPATAAASAIEGVIADPRKYL

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=455, Percent_Identity=25.9340659340659, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI1786259, Length=465, Percent_Identity=37.6344086021505, Blast_Score=284, Evalue=1e-77,
Organism=Escherichia coli, GI87081781, Length=346, Percent_Identity=24.5664739884393, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI2367097, Length=372, Percent_Identity=25.5376344086022, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1787531, Length=358, Percent_Identity=24.8603351955307, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25149337, Length=458, Percent_Identity=26.6375545851528, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI32564738, Length=338, Percent_Identity=27.810650887574, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI25149342, Length=279, Percent_Identity=27.9569892473118, Blast_Score=98, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17568399, Length=386, Percent_Identity=25.3886010362694, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6321429, Length=474, Percent_Identity=37.1308016877637, Blast_Score=281, Evalue=1e-76,
Organism=Saccharomyces cerevisiae, GI6320440, Length=455, Percent_Identity=31.4285714285714, Blast_Score=179, Evalue=9e-46,
Organism=Saccharomyces cerevisiae, GI6322261, Length=460, Percent_Identity=26.5217391304348, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6323335, Length=462, Percent_Identity=26.1904761904762, Blast_Score=132, Evalue=9e-32,
Organism=Drosophila melanogaster, GI281365315, Length=462, Percent_Identity=26.1904761904762, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI17864292, Length=462, Percent_Identity=26.1904761904762, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI28571643, Length=458, Percent_Identity=26.4192139737991, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI161076999, Length=348, Percent_Identity=26.4367816091954, Blast_Score=105, Evalue=8e-23,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 46224; Mature: 46093

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00070 ALDEHYDE_DEHYDR_CYS ; PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKTIAEKIFESHLVDEPFPGTKVLRLDVVLCHEITTPIAIDDLVRRNKDRVFDSTKIKA
CCHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEHHCCCCHHHHHHHHCCHHHHCCCHHHHH
VIDHVTPSKDSKTATQAKILRDWARRHDIPDFFDIGANGVCHALFPEKGFIRPGYTVIMG
HHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHCCCCCEEEEECCCCCCCCCCCEEEEC
DSHTCTHGAFGAFAAGIGTTDLEVGILKGVCAFRQPKTIRFNVIGQLPTGVYAKDVILHI
CCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHH
IGRIGVNGATDRVMEFHGDTIAAMTMDSRMALCNMAIEAGGTSGICMPDMTTVDYLWPFI
HHHCCCCCHHHHHHHHCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
SDEFESKEAALAEYGKWCSDPDASYEQVIDIDVTNLEPTVTFGYKPDQVKPVTELAGTKV
CCHHCHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCHHHHHCCCCC
DQIYLGSCTNGRLEDLRIAAGILKGKKLAPHVRGILSPATPKVYQEALKEGLMEIFMEAG
CEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
FCITNPTCGACLGMSNGVLAEGEVCAATTNRNFMGRMGKGGMVHLMSPATAAASAIEGVI
CEECCCCCHHHHCCCCCCEECCCEEEEECCCCHHCCCCCCCEEEEECCHHHHHHHHHHHH
ADPRKYL
CCHHHCC
>Mature Secondary Structure 
PKTIAEKIFESHLVDEPFPGTKVLRLDVVLCHEITTPIAIDDLVRRNKDRVFDSTKIKA
CHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEHHCCCCHHHHHHHHCCHHHHCCCHHHHH
VIDHVTPSKDSKTATQAKILRDWARRHDIPDFFDIGANGVCHALFPEKGFIRPGYTVIMG
HHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHCCCCCEEEEECCCCCCCCCCCEEEEC
DSHTCTHGAFGAFAAGIGTTDLEVGILKGVCAFRQPKTIRFNVIGQLPTGVYAKDVILHI
CCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHH
IGRIGVNGATDRVMEFHGDTIAAMTMDSRMALCNMAIEAGGTSGICMPDMTTVDYLWPFI
HHHCCCCCHHHHHHHHCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
SDEFESKEAALAEYGKWCSDPDASYEQVIDIDVTNLEPTVTFGYKPDQVKPVTELAGTKV
CCHHCHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCHHHHHCCCCC
DQIYLGSCTNGRLEDLRIAAGILKGKKLAPHVRGILSPATPKVYQEALKEGLMEIFMEAG
CEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
FCITNPTCGACLGMSNGVLAEGEVCAATTNRNFMGRMGKGGMVHLMSPATAAASAIEGVI
CEECCCCCHHHHCCCCCCEECCCEEEEECCCCHHCCCCCCCEEEEECCHHHHHHHHHHHH
ADPRKYL
CCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA