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The map label for this gene is adhE [H]

Identifier: 118580341

GI number: 118580341

Start: 2072715

End: 2074052

Strand: Reverse

Name: adhE [H]

Synonym: Ppro_1924

Alternate gene names: 118580341

Gene position: 2074052-2072715 (Counterclockwise)

Preceding gene: 118580345

Following gene: 118580340

Centisome position: 51.75

GC content: 57.47

Gene sequence:

>1338_bases
GTGGCCGTAGCTGAGAAAATTAATGAAATGGTGCAGAAAGCCAAAGCAGCCGTCGCAGAGTACAAGAAGCTCAACCAGGA
GCAGACCGATGCTATCTGCGAAGCCATCGCCAAGGTAATCGACGCGAAAAAAGAAGAACTTGCACAGGATGCCGTAACGG
AAACCCGCCTCGGTAAGGTAGCTGACAAGACCATCAAGAACTATTTCGGCTCCACGGTTATCTGGAACGACATGAAAGGT
GTCAAGACCGTCGGCGTCATCAAGGACGAAAACAATATCATCGAGATCGCCGAGCCGGTTGGTGTTATTGCTGGCGTATG
TCCGACCACCAACCCGACCTCCACCGTTGCCTTCAAGGTCCTCTCCGCCCTCAAATGCCGCAACGCGATCATCTTCGGCT
TCCACCCCCGCGCCCAGAAGTGCTGTGAAAAAACCGCCCAGCTCTGCCTGGACGCCGCCATCAAAGCCGGCGCTCCGAAA
GATTGCATCCAGTGGATCACCGAGCCTTCCGTCGAGGCTACCAACGCCCTGATGACCAACCCCGGCGTTGATGTCATCCT
GGCAACCGGCGGCGGCGCCATGGTTAGGGCTGCCTACAGCTCCGGCAACCCCGCTTTCGGCGTGGGCGCAGGCGGATGCC
CCGTGTATGTCGCTGCAGATGCCGACATCGACCAGGCCATGTCCGACACCGTTCTCTCCAAGAACTTCGACCACGGCGTT
GTCTGCGCTTCCGAGCAGAACCTGATCTTCGACAACCCCCAGACCGCCGAGAAGGCCATTGCCAAGCTGAAAGAGCTCAA
AACATACGTCTGCAACGACGATGAGAAGAAGAAACTGGAAGCTCTGTACTTCGACCACTATGTCGTCAACGGCGCCGTTG
TTGGTCTGCACGCTGCCAAGATCGCTGAAATGGCCGGCTTCTCCGTACCCGCCGACACCGAAGTACTGCTGGTTGGTCCG
CTGAAAGGCCTGCAGGACGTAGAGCCCATGTGCCGTGAAAAACTCTCCCCGACCCTGGGTTACTACATCGTGGACGGCAA
AGACGCTGCTATCGATCTTTGTGAAAAACTGCTGCAGGGTGGTGGCGCTGGTCACACCGCTGGTATCCACTCCTCTAACG
AAGCAACCTGCCTCCAGTTTGCACAGACTGTTGACGCCAACCGCGTTACCTTCAACTCCCCGACCAGCCACGGCGCCATC
GGCGGCCTGTACAATGTTCTGATTCCCTCCCTGACCCTCGGTTGCGGCTCCAGGGGCAAGAACATCACCAGCGACAACGT
CAGCTTCCGTAACCTGGTTAACATCAAGCGCGCTGCACGTCGTGTTGTCCAGGGCTAA

Upstream 100 bases:

>100_bases
GATGTTAGCGCTATAGGGCAGTGTTCGCTGTTTGTTGACGTTGATTTGAACCTGCATCTGTAGGGCTTGTCGCATACCAA
AAAACCAGGAGGAACAACAC

Downstream 100 bases:

>100_bases
TCACCAGCACTGAATTAATGCGCTAACGTATCATCTGCCGTACATTCCGGCAGGCTCTCTGTTGAGCCTGCCGGATTTTT
TGTCTTTCCTGTGTTCCCCT

Product: aldehyde dehydrogenase

Products: NA

Alternate protein names: Alcohol dehydrogenase; ADH; Acetaldehyde dehydrogenase [acetylating]; ACDH; Pyruvate-formate-lyase deactivase; PFL deactivase [H]

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MAVAEKINEMVQKAKAAVAEYKKLNQEQTDAICEAIAKVIDAKKEELAQDAVTETRLGKVADKTIKNYFGSTVIWNDMKG
VKTVGVIKDENNIIEIAEPVGVIAGVCPTTNPTSTVAFKVLSALKCRNAIIFGFHPRAQKCCEKTAQLCLDAAIKAGAPK
DCIQWITEPSVEATNALMTNPGVDVILATGGGAMVRAAYSSGNPAFGVGAGGCPVYVAADADIDQAMSDTVLSKNFDHGV
VCASEQNLIFDNPQTAEKAIAKLKELKTYVCNDDEKKKLEALYFDHYVVNGAVVGLHAAKIAEMAGFSVPADTEVLLVGP
LKGLQDVEPMCREKLSPTLGYYIVDGKDAAIDLCEKLLQGGGAGHTAGIHSSNEATCLQFAQTVDANRVTFNSPTSHGAI
GGLYNVLIPSLTLGCGSRGKNITSDNVSFRNLVNIKRAARRVVQG

Sequences:

>Translated_445_residues
MAVAEKINEMVQKAKAAVAEYKKLNQEQTDAICEAIAKVIDAKKEELAQDAVTETRLGKVADKTIKNYFGSTVIWNDMKG
VKTVGVIKDENNIIEIAEPVGVIAGVCPTTNPTSTVAFKVLSALKCRNAIIFGFHPRAQKCCEKTAQLCLDAAIKAGAPK
DCIQWITEPSVEATNALMTNPGVDVILATGGGAMVRAAYSSGNPAFGVGAGGCPVYVAADADIDQAMSDTVLSKNFDHGV
VCASEQNLIFDNPQTAEKAIAKLKELKTYVCNDDEKKKLEALYFDHYVVNGAVVGLHAAKIAEMAGFSVPADTEVLLVGP
LKGLQDVEPMCREKLSPTLGYYIVDGKDAAIDLCEKLLQGGGAGHTAGIHSSNEATCLQFAQTVDANRVTFNSPTSHGAI
GGLYNVLIPSLTLGCGSRGKNITSDNVSFRNLVNIKRAARRVVQG
>Mature_444_residues
AVAEKINEMVQKAKAAVAEYKKLNQEQTDAICEAIAKVIDAKKEELAQDAVTETRLGKVADKTIKNYFGSTVIWNDMKGV
KTVGVIKDENNIIEIAEPVGVIAGVCPTTNPTSTVAFKVLSALKCRNAIIFGFHPRAQKCCEKTAQLCLDAAIKAGAPKD
CIQWITEPSVEATNALMTNPGVDVILATGGGAMVRAAYSSGNPAFGVGAGGCPVYVAADADIDQAMSDTVLSKNFDHGVV
CASEQNLIFDNPQTAEKAIAKLKELKTYVCNDDEKKKLEALYFDHYVVNGAVVGLHAAKIAEMAGFSVPADTEVLLVGPL
KGLQDVEPMCREKLSPTLGYYIVDGKDAAIDLCEKLLQGGGAGHTAGIHSSNEATCLQFAQTVDANRVTFNSPTSHGAIG
GLYNVLIPSLTLGCGSRGKNITSDNVSFRNLVNIKRAARRVVQG

Specific function: This enzyme has three activities:ADH, ACDH, and PFL- deactivase. In aerobic conditions it acts as a hydrogen peroxide scavenger. The PFL deactivase activity catalyzes the quenching of the pyruvate-formate-lyase catalyst in an iron, NAD, and CoA dependent

COG id: COG1012

COG function: function code C; NAD-dependent aldehyde dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the iron- containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1787493, Length=445, Percent_Identity=45.6179775280899, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI1788797, Length=383, Percent_Identity=30.8093994778068, Blast_Score=135, Evalue=4e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR012079 [H]

Pfam domain/function: PF00171 Aldedh; PF00465 Fe-ADH [H]

EC number: =1.1.1.1; =1.2.1.10 [H]

Molecular weight: Translated: 47152; Mature: 47021

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVAEKINEMVQKAKAAVAEYKKLNQEQTDAICEAIAKVIDAKKEELAQDAVTETRLGKV
CCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADKTIKNYFGSTVIWNDMKGVKTVGVIKDENNIIEIAEPVGVIAGVCPTTNPTSTVAFKV
HHHHHHHHCCCEEEECCCCCCEEEEEEECCCCEEEEHHHHHHHHCCCCCCCCHHHHHHHH
LSALKCRNAIIFGFHPRAQKCCEKTAQLCLDAAIKAGAPKDCIQWITEPSVEATNALMTN
HHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHEEEC
PGVDVILATGGGAMVRAAYSSGNPAFGVGAGGCPVYVAADADIDQAMSDTVLSKNFDHGV
CCCEEEEECCCCEEEEEEECCCCCCEECCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCE
VCASEQNLIFDNPQTAEKAIAKLKELKTYVCNDDEKKKLEALYFDHYVVNGAVVGLHAAK
EEECCCCEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEEHHHHHH
IAEMAGFSVPADTEVLLVGPLKGLQDVEPMCREKLSPTLGYYIVDGKDAAIDLCEKLLQG
HHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHC
GGAGHTAGIHSSNEATCLQFAQTVDANRVTFNSPTSHGAIGGLYNVLIPSLTLGCGSRGK
CCCCCCCCCCCCCCHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCC
NITSDNVSFRNLVNIKRAARRVVQG
CCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AVAEKINEMVQKAKAAVAEYKKLNQEQTDAICEAIAKVIDAKKEELAQDAVTETRLGKV
CHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADKTIKNYFGSTVIWNDMKGVKTVGVIKDENNIIEIAEPVGVIAGVCPTTNPTSTVAFKV
HHHHHHHHCCCEEEECCCCCCEEEEEEECCCCEEEEHHHHHHHHCCCCCCCCHHHHHHHH
LSALKCRNAIIFGFHPRAQKCCEKTAQLCLDAAIKAGAPKDCIQWITEPSVEATNALMTN
HHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHEEEC
PGVDVILATGGGAMVRAAYSSGNPAFGVGAGGCPVYVAADADIDQAMSDTVLSKNFDHGV
CCCEEEEECCCCEEEEEEECCCCCCEECCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCE
VCASEQNLIFDNPQTAEKAIAKLKELKTYVCNDDEKKKLEALYFDHYVVNGAVVGLHAAK
EEECCCCEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEEHHHHHH
IAEMAGFSVPADTEVLLVGPLKGLQDVEPMCREKLSPTLGYYIVDGKDAAIDLCEKLLQG
HHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHC
GGAGHTAGIHSSNEATCLQFAQTVDANRVTFNSPTSHGAIGGLYNVLIPSLTLGCGSRGK
CCCCCCCCCCCCCCHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCC
NITSDNVSFRNLVNIKRAARRVVQG
CCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]