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The map label for this gene is tnpR [H]

Identifier: 118580331

GI number: 118580331

Start: 2064627

End: 2065184

Strand: Reverse

Name: tnpR [H]

Synonym: Ppro_1914

Alternate gene names: 118580331

Gene position: 2065184-2064627 (Counterclockwise)

Preceding gene: 118580332

Following gene: 118580330

Centisome position: 51.53

GC content: 57.89

Gene sequence:

>558_bases
TTGCAAGGTCAACGTATCGGCTACGTTCGCGTCAGCAGCTTCGACCAGAATCCTGATCGACAACTGGAACAAATAGAAGT
CGGCAAGGTATTCACCGATAAGGCTTCAGGCAAGGACACACAACGTCCCGAACTTGAAAGGCTGCTGGCCTTCGTCCGCG
AGGGCGACACCGTGGTGGTGCATAGCATGGACAGGTTGGCACGCAACCTTGATGACCTGCGCCGCATCGTCCAAGGGCTG
ACACAACGGGGCGTGCGCATGGAGTTCGTCAAAGAAGGGCTAACGTTCACCGGCGAGGACTCACCGATGGCCAATCTGAT
GCTGTCGGTCATGGGAGCCTTTGCTGAGTTCGAGCGCGCTCTGATCCGCGAACGTCAGCGCGAGGGAATCGTGCTGGCTA
AGCAGCGCGGGGCCTACCGGGGACGAAAGAAATCGCTGAACAGCGAACAAATTGCCGAGTTGAAACGGCGAGTTGCGGCA
GGCGACCAGAAAACCTTGGTGGCCCGTGACTTCGGCATCAGCCGTGAAACCTTGTACCAGTACCTGCGGGAAGACTGA

Upstream 100 bases:

>100_bases
AGCACAAACAACGTCAGAGTAGGGTATTAATTTATATTTATTGACACATTCAGCCAAGGGTAATAGATCTCATCCTGACA
TTTTTACCTTTGGAGGCATC

Downstream 100 bases:

>100_bases
CCATGCCACGCCGCTCAATCCTGTCCGCCACCGAGCGCGAAAGTCTGCTGACACTGCCAGATGCCAAAGACGAACTGATA
CGGCACTACACGTTCAACGA

Product: resolvase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MQGQRIGYVRVSSFDQNPDRQLEQIEVGKVFTDKASGKDTQRPELERLLAFVREGDTVVVHSMDRLARNLDDLRRIVQGL
TQRGVRMEFVKEGLTFTGEDSPMANLMLSVMGAFAEFERALIRERQREGIVLAKQRGAYRGRKKSLNSEQIAELKRRVAA
GDQKTLVARDFGISRETLYQYLRED

Sequences:

>Translated_185_residues
MQGQRIGYVRVSSFDQNPDRQLEQIEVGKVFTDKASGKDTQRPELERLLAFVREGDTVVVHSMDRLARNLDDLRRIVQGL
TQRGVRMEFVKEGLTFTGEDSPMANLMLSVMGAFAEFERALIRERQREGIVLAKQRGAYRGRKKSLNSEQIAELKRRVAA
GDQKTLVARDFGISRETLYQYLRED
>Mature_185_residues
MQGQRIGYVRVSSFDQNPDRQLEQIEVGKVFTDKASGKDTQRPELERLLAFVREGDTVVVHSMDRLARNLDDLRRIVQGL
TQRGVRMEFVKEGLTFTGEDSPMANLMLSVMGAFAEFERALIRERQREGIVLAKQRGAYRGRKKSLNSEQIAELKRRVAA
GDQKTLVARDFGISRETLYQYLRED

Specific function: Resolvase catalyzes the resolution (a site-specific recombination) of the cointegrated replicon to yield the final transposition products [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=180, Percent_Identity=39.4444444444444, Blast_Score=115, Evalue=1e-27,
Organism=Escherichia coli, GI1787827, Length=186, Percent_Identity=35.4838709677419, Blast_Score=103, Evalue=5e-24,
Organism=Escherichia coli, GI1787638, Length=183, Percent_Identity=35.5191256830601, Blast_Score=102, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 21224; Mature: 21224

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: PS00018 EF_HAND_1 ; PS00397 RECOMBINASES_1 ; PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGQRIGYVRVSSFDQNPDRQLEQIEVGKVFTDKASGKDTQRPELERLLAFVREGDTVVV
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCEEEE
HSMDRLARNLDDLRRIVQGLTQRGVRMEFVKEGLTFTGEDSPMANLMLSVMGAFAEFERA
ECHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LIRERQREGIVLAKQRGAYRGRKKSLNSEQIAELKRRVAAGDQKTLVARDFGISRETLYQ
HHHHHHHCCEEEECCCCCCCCHHHCCCHHHHHHHHHHHHCCCCCEEHHHHCCCCHHHHHH
YLRED
HHHCC
>Mature Secondary Structure
MQGQRIGYVRVSSFDQNPDRQLEQIEVGKVFTDKASGKDTQRPELERLLAFVREGDTVVV
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCEEEE
HSMDRLARNLDDLRRIVQGLTQRGVRMEFVKEGLTFTGEDSPMANLMLSVMGAFAEFERA
ECHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LIRERQREGIVLAKQRGAYRGRKKSLNSEQIAELKRRVAAGDQKTLVARDFGISRETLYQ
HHHHHHHCCEEEECCCCCCCCHHHCCCHHHHHHHHHHHHCCCCCEEHHHHCCCCHHHHHH
YLRED
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6312271 [H]