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The map label for this gene is intA [H]

Identifier: 118580278

GI number: 118580278

Start: 2001971

End: 2003254

Strand: Direct

Name: intA [H]

Synonym: Ppro_1859

Alternate gene names: 118580278

Gene position: 2001971-2003254 (Clockwise)

Preceding gene: 118580266

Following gene: 118580296

Centisome position: 49.95

GC content: 44.78

Gene sequence:

>1284_bases
ATGGCAACCGGAACTCGTTTTACTGATAAATTTATTATGAGTCTGAAACCGGCAGATAAGGAGTATTGGAAGAGGGAGGG
GCAGGGATTCTCAATTCGTGTTTATCCTTCTGGCGAAAAAGCCTGGTATTTTATCTATACATTTGATGGGCGAAAAAAAT
ACATGCGCTTAAAAGATGGGGGCTATCCCGACGTTTCACTGGCAGATGCAAGAGAAGCATTCGACATCGCTAAGGTTAAG
TTGAAAAATGGAATCGACCCCCTCGCAGAACTCGAACAGGCAAGACTTGACCGTAAGCATACGCCGTTTGTGAAAGAGTT
TGTTGACGAGTTTATCAGAATTTACTGTAAAGAGAATAATCGGGGGTGGAAAGAAATCGAACGCGCATTAAAATCAGAGA
TAGTTCCGAGATGGGGAAAGCGAAAACTGACTGACATCAAACGACGAGACCTTGTCCTTGTGTTGGATGAAATCAAAGAA
CGAGGCGCACCTGTAATGGCAAATCGGATTCTTGCGTATACCCGCAAAATGTTTTCGTGGGCATTAGAGCGCGCTGCTCT
GGAAGTTAACCCATTTCTTATGATGAGTAGGCCGAATGATGAAACATCAAGGGAAAGGGTATTATCTGAAAAAGAGATTA
AGACCTTTTGGCATAACCTCGATGAATGTAAAATGAGTGACAGCATCAAAAGAGCATTAAAGTTGATACTTGTAACGGGT
CAGCGCCCTGGGGAAATTATTGGCATGCACCGTAACGAAATTGATGGTGAATGGTGGGAGATTCCTGCCGCACGTTCCAA
GAACAAACAGGCGCATCGTGTTTTCCTGACAATAACAGCTAAACAGCTAATTGGTGATGCGTCTGGATTTATTTTTGAAT
CACCAGCTAATCCAGCTAAACCTGACGAAACGCCTCCAAAGCCTGCCAAACCTTATGAAGTTAGAACCATGACCCACGAC
ATAAAGCAGAATCTTCCGCATACGCCTCACAGTAAGGTGATTGACCGTCTAAAGGTACCGCATTTTACTCCTCATGATTT
GCGCCGAACTGCCGCGACTAGATGGGCAGAAATGGAGATTACCGGTGATATGATTGACCGGTTACAGAACCACATTACAA
GGCAAAAGCAGGGTGTTGGCCATGTTTACAATCGTTACTCGTACGCTAGGGAAAAACAGCAGGCACTTGAAGCATGGGAG
CAAAAGCTTAACAGTATCATCACCAGCATCGGATCAGACAGCGTGGTCCTGATCGACAAAGACAGCGACACAAATGCCGC
GTAA

Upstream 100 bases:

>100_bases
GGGGATACGGAAAAAGACTGGTATATCTTGTAATGTTAGTATATTATGCTTTATAAGGATACGCTATAGGATACGGTAGT
ATATTTGAAGGGAAAATACA

Downstream 100 bases:

>100_bases
ACATTTTTTATCGTAAGACGAAATGGTAATAAGGATTATGCGCAGCTGAAACCAACAATGTAGAAACAAGGGGCTGCGAT
TCGCGGCCCTTATTTTTTTC

Product: phage integrase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MATGTRFTDKFIMSLKPADKEYWKREGQGFSIRVYPSGEKAWYFIYTFDGRKKYMRLKDGGYPDVSLADAREAFDIAKVK
LKNGIDPLAELEQARLDRKHTPFVKEFVDEFIRIYCKENNRGWKEIERALKSEIVPRWGKRKLTDIKRRDLVLVLDEIKE
RGAPVMANRILAYTRKMFSWALERAALEVNPFLMMSRPNDETSRERVLSEKEIKTFWHNLDECKMSDSIKRALKLILVTG
QRPGEIIGMHRNEIDGEWWEIPAARSKNKQAHRVFLTITAKQLIGDASGFIFESPANPAKPDETPPKPAKPYEVRTMTHD
IKQNLPHTPHSKVIDRLKVPHFTPHDLRRTAATRWAEMEITGDMIDRLQNHITRQKQGVGHVYNRYSYAREKQQALEAWE
QKLNSIITSIGSDSVVLIDKDSDTNAA

Sequences:

>Translated_427_residues
MATGTRFTDKFIMSLKPADKEYWKREGQGFSIRVYPSGEKAWYFIYTFDGRKKYMRLKDGGYPDVSLADAREAFDIAKVK
LKNGIDPLAELEQARLDRKHTPFVKEFVDEFIRIYCKENNRGWKEIERALKSEIVPRWGKRKLTDIKRRDLVLVLDEIKE
RGAPVMANRILAYTRKMFSWALERAALEVNPFLMMSRPNDETSRERVLSEKEIKTFWHNLDECKMSDSIKRALKLILVTG
QRPGEIIGMHRNEIDGEWWEIPAARSKNKQAHRVFLTITAKQLIGDASGFIFESPANPAKPDETPPKPAKPYEVRTMTHD
IKQNLPHTPHSKVIDRLKVPHFTPHDLRRTAATRWAEMEITGDMIDRLQNHITRQKQGVGHVYNRYSYAREKQQALEAWE
QKLNSIITSIGSDSVVLIDKDSDTNAA
>Mature_426_residues
ATGTRFTDKFIMSLKPADKEYWKREGQGFSIRVYPSGEKAWYFIYTFDGRKKYMRLKDGGYPDVSLADAREAFDIAKVKL
KNGIDPLAELEQARLDRKHTPFVKEFVDEFIRIYCKENNRGWKEIERALKSEIVPRWGKRKLTDIKRRDLVLVLDEIKER
GAPVMANRILAYTRKMFSWALERAALEVNPFLMMSRPNDETSRERVLSEKEIKTFWHNLDECKMSDSIKRALKLILVTGQ
RPGEIIGMHRNEIDGEWWEIPAARSKNKQAHRVFLTITAKQLIGDASGFIFESPANPAKPDETPPKPAKPYEVRTMTHDI
KQNLPHTPHSKVIDRLKVPHFTPHDLRRTAATRWAEMEITGDMIDRLQNHITRQKQGVGHVYNRYSYAREKQQALEAWEQ
KLNSIITSIGSDSVVLIDKDSDTNAA

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788974, Length=447, Percent_Identity=24.8322147651007, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI145693166, Length=432, Percent_Identity=25.462962962963, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1788690, Length=186, Percent_Identity=26.3440860215054, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 49556; Mature: 49425

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATGTRFTDKFIMSLKPADKEYWKREGQGFSIRVYPSGEKAWYFIYTFDGRKKYMRLKDG
CCCCCCHHHHHHHHCCCCCHHHHHHCCCCCEEEEEECCCCEEEEEEEECCCEEEEEECCC
GYPDVSLADAREAFDIAKVKLKNGIDPLAELEQARLDRKHTPFVKEFVDEFIRIYCKENN
CCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCC
RGWKEIERALKSEIVPRWGKRKLTDIKRRDLVLVLDEIKERGAPVMANRILAYTRKMFSW
CCHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
ALERAALEVNPFLMMSRPNDETSRERVLSEKEIKTFWHNLDECKMSDSIKRALKLILVTG
HHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEC
QRPGEIIGMHRNEIDGEWWEIPAARSKNKQAHRVFLTITAKQLIGDASGFIFESPANPAK
CCCCCEEECCCCCCCCCCEECCCCCCCCCCEEEEEEEEEHHHHHCCCCCCEEECCCCCCC
PDETPPKPAKPYEVRTMTHDIKQNLPHTPHSKVIDRLKVPHFTPHDLRRTAATRWAEMEI
CCCCCCCCCCCEEHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHEEEE
TGDMIDRLQNHITRQKQGVGHVYNRYSYAREKQQALEAWEQKLNSIITSIGSDSVVLIDK
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
DSDTNAA
CCCCCCC
>Mature Secondary Structure 
ATGTRFTDKFIMSLKPADKEYWKREGQGFSIRVYPSGEKAWYFIYTFDGRKKYMRLKDG
CCCCCHHHHHHHHCCCCCHHHHHHCCCCCEEEEEECCCCEEEEEEEECCCEEEEEECCC
GYPDVSLADAREAFDIAKVKLKNGIDPLAELEQARLDRKHTPFVKEFVDEFIRIYCKENN
CCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCC
RGWKEIERALKSEIVPRWGKRKLTDIKRRDLVLVLDEIKERGAPVMANRILAYTRKMFSW
CCHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
ALERAALEVNPFLMMSRPNDETSRERVLSEKEIKTFWHNLDECKMSDSIKRALKLILVTG
HHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEC
QRPGEIIGMHRNEIDGEWWEIPAARSKNKQAHRVFLTITAKQLIGDASGFIFESPANPAK
CCCCCEEECCCCCCCCCCEECCCCCCCCCCEEEEEEEEEHHHHHCCCCCCEEECCCCCCC
PDETPPKPAKPYEVRTMTHDIKQNLPHTPHSKVIDRLKVPHFTPHDLRRTAATRWAEMEI
CCCCCCCCCCCEEHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHEEEE
TGDMIDRLQNHITRQKQGVGHVYNRYSYAREKQQALEAWEQKLNSIITSIGSDSVVLIDK
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
DSDTNAA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7511583; 9278503; 9205837; 7524073 [H]