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The map label for this gene is pyk [H]

Identifier: 118580272

GI number: 118580272

Start: 1990771

End: 1992192

Strand: Reverse

Name: pyk [H]

Synonym: Ppro_1852

Alternate gene names: 118580272

Gene position: 1992192-1990771 (Counterclockwise)

Preceding gene: 118580273

Following gene: 118580271

Centisome position: 49.71

GC content: 61.95

Gene sequence:

>1422_bases
GTGAAACTGCCAGACCACAAAACGAAAATCATCTGCACCATCGGTCCGGCTTCTTCAAGCCGGAAGGTCATGGAGGAGAT
GCTTATGGCCGGAATGAACGTGGCCCGGCTCAATTTTTCCCATGGTTCCTTCGAGAGCCACCGCCAGGTGATTGGCGACC
TGCGCGCCGCTTCCCGTGCCACCGGCAAGCGGCTGGCCATCATGGCGGACCTCCCTGGACCGAAGATGCGCATCGGCACG
CTCAAGGATGAACCGGTGGAACTGAAACCGGGGGATTCCATCGTGCTGACCAGCGATGACATCGACGGTGATCGGGAGAG
GGTGTCAGTGTCTTTCAAGCGACTTCCCCAGGTGGTCACCCCCGGTAATGCCCTCTACCTCAACGATGGCATCATCCAGA
TCGAAGTCGTGTCCGTCATTGGCAACGACGTTGCCTGCAGGGTCGTGGTGGGAGGAGAACTCCGTTCACGCAAGGGGCTC
AACCTTCCGGATATTGATCTCGGCATCAGCGCTTTCACGGAACGTGATCATGAGTGCCTGACGTTTGCCGCCCAGGAGGG
GGTGGACGCCGTCAGCCAGTCTTTCGTGGAAAACGGCGACGACATCAGGGCGGTGCGGCGTGCGGCCGAGGATATGGACT
ACCACCCCTTTATCATCGCCAAGATCGAGCGCTCCAACGCCCTGGAACAGATGGATGATATCCTCGATGCCTCCGACGGC
ATCATGATCGCCCGGGGAGACCTGGGGGTGGAGGTGCCCATAGAGAGGATCGCCATCATCCAGAAGGATTTGATGCGCAA
GGCCAACAGGCGCGCCAAACCGGTCATCACGGCAACCCAGATGCTGGAGTCCATGACCGAGACCCGACGCCCCACCCGTG
CCGAGGCTACGGATGTGGCTAACGCCATCCTGGACGGCACCGATTGCGTCATGCTCTCGGGAGAGTCCGCCATGGGGAAG
TATCCCGTGGATGCCGTGGCCATGCTCGCCAGCATAGCGGCTGCCGTGGAGCCCCACAAGCGGCAGATCACGGTGAAAGA
ACTGTACGGGGGAGGGGATTTCAAGGGCACGCTCAAGCCGGCGCACCTGATCGCCGTGTCGGTGGAGGCGAGCGTTACCT
ACGCATCCCCGGCGGCGGTGTTCGCCCCGACCCGCAGTGGCGCTACCGCCCGGAGCCTGTCGCTCTTCAGGCTGCCGGTC
TGGGTCGCGGCGGTGAGTTCCCGCCACCAGACTTGCCAGGACCTGGCCTTCTCCTACGGCGTGTTTCCCGTGCACGAGAC
AGGGCATCCCGAGGATTGGGATGCCTTTGTGCGCAGGTGGGTTGAGATGTATCGGGCGCCGGGAGATATTGCCATCCTGA
CGGAGGGGCCCTCCACACGGCACCCCGACGCCCACAACCGGATGGAAATCATCCAACTGTAA

Upstream 100 bases:

>100_bases
AGCGAGCGGTGAGCGATCTGCGGGGGACCAACAGCACACTCTTCACGGGGTAATTCGCCTGCCGTGATAGTGCCGCAACC
AGAGAATCAGGAGGTGCATC

Downstream 100 bases:

>100_bases
CCTGCAATGGCAGGCAAGGCAGATCTTCTCATGCATGGACCGTGTCCATGGCAACGCAGGCGTACCGACATATTCCAGGG
AGGTAAGAACGATGAATGCA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MKLPDHKTKIICTIGPASSSRKVMEEMLMAGMNVARLNFSHGSFESHRQVIGDLRAASRATGKRLAIMADLPGPKMRIGT
LKDEPVELKPGDSIVLTSDDIDGDRERVSVSFKRLPQVVTPGNALYLNDGIIQIEVVSVIGNDVACRVVVGGELRSRKGL
NLPDIDLGISAFTERDHECLTFAAQEGVDAVSQSFVENGDDIRAVRRAAEDMDYHPFIIAKIERSNALEQMDDILDASDG
IMIARGDLGVEVPIERIAIIQKDLMRKANRRAKPVITATQMLESMTETRRPTRAEATDVANAILDGTDCVMLSGESAMGK
YPVDAVAMLASIAAAVEPHKRQITVKELYGGGDFKGTLKPAHLIAVSVEASVTYASPAAVFAPTRSGATARSLSLFRLPV
WVAAVSSRHQTCQDLAFSYGVFPVHETGHPEDWDAFVRRWVEMYRAPGDIAILTEGPSTRHPDAHNRMEIIQL

Sequences:

>Translated_473_residues
MKLPDHKTKIICTIGPASSSRKVMEEMLMAGMNVARLNFSHGSFESHRQVIGDLRAASRATGKRLAIMADLPGPKMRIGT
LKDEPVELKPGDSIVLTSDDIDGDRERVSVSFKRLPQVVTPGNALYLNDGIIQIEVVSVIGNDVACRVVVGGELRSRKGL
NLPDIDLGISAFTERDHECLTFAAQEGVDAVSQSFVENGDDIRAVRRAAEDMDYHPFIIAKIERSNALEQMDDILDASDG
IMIARGDLGVEVPIERIAIIQKDLMRKANRRAKPVITATQMLESMTETRRPTRAEATDVANAILDGTDCVMLSGESAMGK
YPVDAVAMLASIAAAVEPHKRQITVKELYGGGDFKGTLKPAHLIAVSVEASVTYASPAAVFAPTRSGATARSLSLFRLPV
WVAAVSSRHQTCQDLAFSYGVFPVHETGHPEDWDAFVRRWVEMYRAPGDIAILTEGPSTRHPDAHNRMEIIQL
>Mature_473_residues
MKLPDHKTKIICTIGPASSSRKVMEEMLMAGMNVARLNFSHGSFESHRQVIGDLRAASRATGKRLAIMADLPGPKMRIGT
LKDEPVELKPGDSIVLTSDDIDGDRERVSVSFKRLPQVVTPGNALYLNDGIIQIEVVSVIGNDVACRVVVGGELRSRKGL
NLPDIDLGISAFTERDHECLTFAAQEGVDAVSQSFVENGDDIRAVRRAAEDMDYHPFIIAKIERSNALEQMDDILDASDG
IMIARGDLGVEVPIERIAIIQKDLMRKANRRAKPVITATQMLESMTETRRPTRAEATDVANAILDGTDCVMLSGESAMGK
YPVDAVAMLASIAAAVEPHKRQITVKELYGGGDFKGTLKPAHLIAVSVEASVTYASPAAVFAPTRSGATARSLSLFRLPV
WVAAVSSRHQTCQDLAFSYGVFPVHETGHPEDWDAFVRRWVEMYRAPGDIAILTEGPSTRHPDAHNRMEIIQL

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=432, Percent_Identity=39.8148148148148, Blast_Score=290, Evalue=2e-78,
Organism=Homo sapiens, GI33286420, Length=432, Percent_Identity=39.8148148148148, Blast_Score=290, Evalue=2e-78,
Organism=Homo sapiens, GI33286418, Length=431, Percent_Identity=39.907192575406, Blast_Score=289, Evalue=4e-78,
Organism=Homo sapiens, GI10835121, Length=473, Percent_Identity=38.6892177589852, Blast_Score=286, Evalue=3e-77,
Organism=Homo sapiens, GI32967597, Length=473, Percent_Identity=38.6892177589852, Blast_Score=286, Evalue=3e-77,
Organism=Homo sapiens, GI310128732, Length=285, Percent_Identity=38.9473684210526, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI310128730, Length=285, Percent_Identity=38.9473684210526, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI310128736, Length=229, Percent_Identity=35.8078602620087, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI310128734, Length=229, Percent_Identity=35.8078602620087, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI310128738, Length=210, Percent_Identity=33.3333333333333, Blast_Score=116, Evalue=4e-26,
Organism=Escherichia coli, GI1787965, Length=463, Percent_Identity=40.1727861771058, Blast_Score=311, Evalue=4e-86,
Organism=Escherichia coli, GI1788160, Length=471, Percent_Identity=38.4288747346072, Blast_Score=277, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI17544584, Length=448, Percent_Identity=39.5089285714286, Blast_Score=319, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI71984413, Length=442, Percent_Identity=40.4977375565611, Blast_Score=288, Evalue=5e-78,
Organism=Caenorhabditis elegans, GI17506829, Length=442, Percent_Identity=40.4977375565611, Blast_Score=288, Evalue=6e-78,
Organism=Caenorhabditis elegans, GI71984406, Length=442, Percent_Identity=40.4977375565611, Blast_Score=287, Evalue=8e-78,
Organism=Caenorhabditis elegans, GI17506831, Length=442, Percent_Identity=40.4977375565611, Blast_Score=287, Evalue=8e-78,
Organism=Saccharomyces cerevisiae, GI6324923, Length=459, Percent_Identity=37.2549019607843, Blast_Score=271, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6319279, Length=438, Percent_Identity=36.0730593607306, Blast_Score=266, Evalue=8e-72,
Organism=Drosophila melanogaster, GI24648964, Length=438, Percent_Identity=38.3561643835616, Blast_Score=292, Evalue=4e-79,
Organism=Drosophila melanogaster, GI28571814, Length=438, Percent_Identity=38.3561643835616, Blast_Score=292, Evalue=4e-79,
Organism=Drosophila melanogaster, GI24648966, Length=416, Percent_Identity=37.9807692307692, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24581235, Length=432, Percent_Identity=32.1759259259259, Blast_Score=229, Evalue=3e-60,
Organism=Drosophila melanogaster, GI24646914, Length=268, Percent_Identity=35.8208955223881, Blast_Score=151, Evalue=1e-36,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51562; Mature: 51562

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLPDHKTKIICTIGPASSSRKVMEEMLMAGMNVARLNFSHGSFESHRQVIGDLRAASRA
CCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHC
TGKRLAIMADLPGPKMRIGTLKDEPVELKPGDSIVLTSDDIDGDRERVSVSFKRLPQVVT
CCCEEEEEECCCCCCEEEECCCCCCEEECCCCEEEEECCCCCCCHHHEEEEHHHCCCEEC
PGNALYLNDGIIQIEVVSVIGNDVACRVVVGGELRSRKGLNLPDIDLGISAFTERDHECL
CCCEEEEECCEEEEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHCCCCHHH
TFAAQEGVDAVSQSFVENGDDIRAVRRAAEDMDYHPFIIAKIERSNALEQMDDILDASDG
HHHHHHCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHCCCCC
IMIARGDLGVEVPIERIAIIQKDLMRKANRRAKPVITATQMLESMTETRRPTRAEATDVA
EEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHH
NAILDGTDCVMLSGESAMGKYPVDAVAMLASIAAAVEPHKRQITVKELYGGGDFKGTLKP
HHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCHHHEEEEEHHCCCCCCCCCCCC
AHLIAVSVEASVTYASPAAVFAPTRSGATARSLSLFRLPVWVAAVSSRHQTCQDLAFSYG
EEEEEEEEEEEEEECCCCEEEECCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
VFPVHETGHPEDWDAFVRRWVEMYRAPGDIAILTEGPSTRHPDAHNRMEIIQL
CEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCEEEEEC
>Mature Secondary Structure
MKLPDHKTKIICTIGPASSSRKVMEEMLMAGMNVARLNFSHGSFESHRQVIGDLRAASRA
CCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHC
TGKRLAIMADLPGPKMRIGTLKDEPVELKPGDSIVLTSDDIDGDRERVSVSFKRLPQVVT
CCCEEEEEECCCCCCEEEECCCCCCEEECCCCEEEEECCCCCCCHHHEEEEHHHCCCEEC
PGNALYLNDGIIQIEVVSVIGNDVACRVVVGGELRSRKGLNLPDIDLGISAFTERDHECL
CCCEEEEECCEEEEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHCCCCHHH
TFAAQEGVDAVSQSFVENGDDIRAVRRAAEDMDYHPFIIAKIERSNALEQMDDILDASDG
HHHHHHCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHCCCCC
IMIARGDLGVEVPIERIAIIQKDLMRKANRRAKPVITATQMLESMTETRRPTRAEATDVA
EEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHH
NAILDGTDCVMLSGESAMGKYPVDAVAMLASIAAAVEPHKRQITVKELYGGGDFKGTLKP
HHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCHHHEEEEEHHCCCCCCCCCCCC
AHLIAVSVEASVTYASPAAVFAPTRSGATARSLSLFRLPVWVAAVSSRHQTCQDLAFSYG
EEEEEEEEEEEEEECCCCEEEECCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
VFPVHETGHPEDWDAFVRRWVEMYRAPGDIAILTEGPSTRHPDAHNRMEIIQL
CEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA