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The map label for this gene is hprA [H]

Identifier: 118580230

GI number: 118580230

Start: 1938509

End: 1939465

Strand: Reverse

Name: hprA [H]

Synonym: Ppro_1810

Alternate gene names: 118580230

Gene position: 1939465-1938509 (Counterclockwise)

Preceding gene: 118580231

Following gene: 118580229

Centisome position: 48.39

GC content: 62.49

Gene sequence:

>957_bases
ATGAACAGTACGGTATTTCTCAACGCGGCCAGGCTCGATTTCGACGGCAAGCTCGATTTCTCTCCCTTGTCCGATCTGAC
GGCATTCACCAGATATGACAACAGCAGCGACGAGGAGATCCCCTCCCGCGTGGAAGGGCAGACCATCGTCATCACCAAGG
AACTGCCCCTGGGCAGGGAGCTGATCCACTGTTTCCCGGCATCGGTCAAACTGATCTGCGAGGCCGGCACCGGCTACAAC
AACATCGACATCGCCGCGGCCCGCTCCCGGGGGATCGGCGTCTGCAACGTGCCCAGCTACAGCACCGACGCGGTGGCCCA
GCTGGCCATCACCTTCATGCTCAACCTGAGCGCCTCCCTGGTGCAGCAGCAGACCATGCTGCGCCGGGGCAACCTGGACA
ATTTCCAGAAGAGCCTGCAGCTGCCCCACTTTGAACTGAACGGGAAAACCCTGGGGGTGATCGGCTTCGGTGAGATCGGG
CGCCGGGTAATCGCCATCGCCCGCACCCTGGGAATGAAGATCATCGTCCATAGCCGCACCCCCCGACCGGAGCTGGACCC
CGATCTGCGTTTCGTCTCCCTGGAGGAACTGCTGGCCACCAGCGACTTCGTCTCCCTACACTGCCCCCTGAACGACGCCA
CCCGGCATGTGATCAACGCCGAGCGGCTGGAAATGATGAAGCCCACCGCCTTCATCATCAACACCTCCCGCGGCCCGCTG
ATCCACGAACCGGCCCTGAGCCAGGCCCTGACCAGGGGAACCATCGCCGGCGCCGGACTGGACGTGCAGGAGCAGGAACC
TCCCGAGCCGGGCGACCCGCTCTACTCCCTGGAAAACGTGATCCTGACCCCGCACATCGGCTGGAAACGCCTGGAGACCA
GGCAGCGACTGATCGGACTGACCGCCGCCAACATCGATGCCTTCATCAAAGACGCTGCGATCAATATCGTGAATTGA

Upstream 100 bases:

>100_bases
CTTCGAGTCGTGGGGTAAGGGACGGCCCGCCAATCGGTGGGCCGTTTATTTTTTGCGGTATGCACCGGAGATGGTGGCAG
GCTCCGGCAGGGAGGGCTCC

Downstream 100 bases:

>100_bases
ACAGAACGGCGCATGATAGTGTGGTAACCAGGGGGGAAGCCTTGCAGCACAGCGGGCTTCCCCCCTTTTCGTTGCCGCGC
CCGGCAGCGTCACCCCCCCG

Product: NAD-binding D-isomer specific 2-hydroxyacid dehydrogenase

Products: NA

Alternate protein names: GDH; Glyoxylate reductase; Hydroxypyruvate dehydrogenase; NADH-dependent hydroxypyruvate reductase; HPR; HPR-A [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MNSTVFLNAARLDFDGKLDFSPLSDLTAFTRYDNSSDEEIPSRVEGQTIVITKELPLGRELIHCFPASVKLICEAGTGYN
NIDIAAARSRGIGVCNVPSYSTDAVAQLAITFMLNLSASLVQQQTMLRRGNLDNFQKSLQLPHFELNGKTLGVIGFGEIG
RRVIAIARTLGMKIIVHSRTPRPELDPDLRFVSLEELLATSDFVSLHCPLNDATRHVINAERLEMMKPTAFIINTSRGPL
IHEPALSQALTRGTIAGAGLDVQEQEPPEPGDPLYSLENVILTPHIGWKRLETRQRLIGLTAANIDAFIKDAAINIVN

Sequences:

>Translated_318_residues
MNSTVFLNAARLDFDGKLDFSPLSDLTAFTRYDNSSDEEIPSRVEGQTIVITKELPLGRELIHCFPASVKLICEAGTGYN
NIDIAAARSRGIGVCNVPSYSTDAVAQLAITFMLNLSASLVQQQTMLRRGNLDNFQKSLQLPHFELNGKTLGVIGFGEIG
RRVIAIARTLGMKIIVHSRTPRPELDPDLRFVSLEELLATSDFVSLHCPLNDATRHVINAERLEMMKPTAFIINTSRGPL
IHEPALSQALTRGTIAGAGLDVQEQEPPEPGDPLYSLENVILTPHIGWKRLETRQRLIGLTAANIDAFIKDAAINIVN
>Mature_318_residues
MNSTVFLNAARLDFDGKLDFSPLSDLTAFTRYDNSSDEEIPSRVEGQTIVITKELPLGRELIHCFPASVKLICEAGTGYN
NIDIAAARSRGIGVCNVPSYSTDAVAQLAITFMLNLSASLVQQQTMLRRGNLDNFQKSLQLPHFELNGKTLGVIGFGEIG
RRVIAIARTLGMKIIVHSRTPRPELDPDLRFVSLEELLATSDFVSLHCPLNDATRHVINAERLEMMKPTAFIINTSRGPL
IHEPALSQALTRGTIAGAGLDVQEQEPPEPGDPLYSLENVILTPHIGWKRLETRQRLIGLTAANIDAFIKDAAINIVN

Specific function: Plays a central role in assimilation of carbon. It converts hydroxypyruvate to glycerate as a key step in the serine cycle, and may also play an important role in C2 reactions, by interconverting glyoxylate and glycolate [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI145580578, Length=319, Percent_Identity=34.1692789968652, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI4557499, Length=319, Percent_Identity=34.1692789968652, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI4557497, Length=319, Percent_Identity=32.9153605015674, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI61743967, Length=319, Percent_Identity=32.9153605015674, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI145580575, Length=319, Percent_Identity=34.1692789968652, Blast_Score=148, Evalue=5e-36,
Organism=Homo sapiens, GI6912396, Length=311, Percent_Identity=30.5466237942122, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI23308577, Length=230, Percent_Identity=33.4782608695652, Blast_Score=127, Evalue=1e-29,
Organism=Escherichia coli, GI1789279, Length=230, Percent_Identity=37.3913043478261, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI87082289, Length=247, Percent_Identity=35.2226720647773, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1787645, Length=200, Percent_Identity=37.5, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI1788660, Length=232, Percent_Identity=28.448275862069, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI87081824, Length=154, Percent_Identity=32.4675324675325, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17532191, Length=292, Percent_Identity=29.7945205479452, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI25147481, Length=289, Percent_Identity=30.7958477508651, Blast_Score=124, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6324055, Length=253, Percent_Identity=35.9683794466403, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6320925, Length=238, Percent_Identity=35.2941176470588, Blast_Score=136, Evalue=5e-33,
Organism=Saccharomyces cerevisiae, GI6322116, Length=238, Percent_Identity=34.8739495798319, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6324964, Length=253, Percent_Identity=25.296442687747, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6324980, Length=175, Percent_Identity=25.7142857142857, Blast_Score=72, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24646446, Length=283, Percent_Identity=33.2155477031802, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24646448, Length=283, Percent_Identity=33.2155477031802, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24646452, Length=283, Percent_Identity=33.2155477031802, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24646450, Length=283, Percent_Identity=33.2155477031802, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI62472511, Length=283, Percent_Identity=33.2155477031802, Blast_Score=141, Evalue=7e-34,
Organism=Drosophila melanogaster, GI24585516, Length=276, Percent_Identity=32.6086956521739, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI19921140, Length=252, Percent_Identity=30.952380952381, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI28571528, Length=250, Percent_Identity=32.8, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI28574286, Length=253, Percent_Identity=29.6442687747036, Blast_Score=118, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24585514, Length=257, Percent_Identity=30.7392996108949, Blast_Score=114, Evalue=8e-26,
Organism=Drosophila melanogaster, GI28574282, Length=257, Percent_Identity=30.7392996108949, Blast_Score=114, Evalue=8e-26,
Organism=Drosophila melanogaster, GI28574284, Length=257, Percent_Identity=30.7392996108949, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI45552429, Length=257, Percent_Identity=30.7392996108949, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI45551003, Length=257, Percent_Identity=30.7392996108949, Blast_Score=114, Evalue=1e-25,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.29 [H]

Molecular weight: Translated: 34894; Mature: 34894

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00670 D_2_HYDROXYACID_DH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSTVFLNAARLDFDGKLDFSPLSDLTAFTRYDNSSDEEIPSRVEGQTIVITKELPLGRE
CCCEEEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHCCCEEEEEECCCCCHH
LIHCFPASVKLICEAGTGYNNIDIAAARSRGIGVCNVPSYSTDAVAQLAITFMLNLSASL
HHHHCCCCEEEEEECCCCCCCEEEEEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHH
VQQQTMLRRGNLDNFQKSLQLPHFELNGKTLGVIGFGEIGRRVIAIARTLGMKIIVHSRT
HHHHHHHHHCCHHHHHHHHCCCCEEECCCEEEEEECHHHHHHHHHHHHHHCEEEEEECCC
PRPELDPDLRFVSLEELLATSDFVSLHCPLNDATRHVINAERLEMMKPTAFIINTSRGPL
CCCCCCCCCEEEEHHHHHHCCCEEEEECCCCHHHHHHHCHHHHHHCCCEEEEEECCCCCE
IHEPALSQALTRGTIAGAGLDVQEQEPPEPGDPLYSLENVILTPHIGWKRLETRQRLIGL
EECHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHCCCEEECCCCCHHHHHHHHHHHEE
TAANIDAFIKDAAINIVN
EHHHHHHHHHHCEEEECC
>Mature Secondary Structure
MNSTVFLNAARLDFDGKLDFSPLSDLTAFTRYDNSSDEEIPSRVEGQTIVITKELPLGRE
CCCEEEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHCCCEEEEEECCCCCHH
LIHCFPASVKLICEAGTGYNNIDIAAARSRGIGVCNVPSYSTDAVAQLAITFMLNLSASL
HHHHCCCCEEEEEECCCCCCCEEEEEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHH
VQQQTMLRRGNLDNFQKSLQLPHFELNGKTLGVIGFGEIGRRVIAIARTLGMKIIVHSRT
HHHHHHHHHCCHHHHHHHHCCCCEEECCCEEEEEECHHHHHHHHHHHHHHCEEEEEECCC
PRPELDPDLRFVSLEELLATSDFVSLHCPLNDATRHVINAERLEMMKPTAFIINTSRGPL
CCCCCCCCCEEEEHHHHHHCCCEEEEECCCCHHHHHHHCHHHHHHCCCEEEEEECCCCCE
IHEPALSQALTRGTIAGAGLDVQEQEPPEPGDPLYSLENVILTPHIGWKRLETRQRLIGL
EECHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHCCCEEECCCCCHHHHHHHHHHHEE
TAANIDAFIKDAAINIVN
EHHHHHHHHHHCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8144463; 1729225; 1657886 [H]