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The map label for this gene is tgt [H]

Identifier: 118580220

GI number: 118580220

Start: 1926124

End: 1927248

Strand: Reverse

Name: tgt [H]

Synonym: Ppro_1800

Alternate gene names: 118580220

Gene position: 1927248-1926124 (Counterclockwise)

Preceding gene: 118580221

Following gene: 118580219

Centisome position: 48.09

GC content: 60.98

Gene sequence:

>1125_bases
TTGTCGACTTCTCTTTTCACACTCCTTCACCGGGATGCCTCCTGCGGGGCGCGGCTTGGCAGCCTGAATACCCTGCACGG
CGCCATTGAGACCCCCATCTTCATGCCGGTGGGTACCAACGCCACCGTCAAGGCCATGACTCCCGATGATCTGCTGGCGG
TCAACGCCCAGATCATCCTCTCCAACACCTACCACCTCTACCTCCGTCCGGGGCACCGACTGGTGGAGCAGTTGGGCGGG
TTGCACTCCTTCATGAACTGGCACCGCCCGATCCTGACCGACAGCGGCGGATTCCAGGTCTTCAGCCTGGGGGAGTTGCG
CAAGATCAGCGAGGAAGGGGTCAGGTTCCAGTCCCATCTGGACGGTTCCTATCACTTCCTGACCCCGGAACTGTCCATAG
GGATCCAGGAGGCGTTGGGTGCGGATATCATCATGTGCTTCGACGAGTGCCCCCCGGCCACGGCGGAGTACGACTACGTC
AGCCGCTCCCTGGAGATGACCACCCGCTGGGCAAGGCGCTGCAAACAGGCCCACCGGCGTGAGGGACAGCAGTTGTTCGG
CATCATTCAGGGGGGAATGCATTACGACCTGCGTGCCCGCAGCCTGGAGCAGATCTTTGAGATCGGCTTTGACGGCTATG
CTTTGGGCGGCCTCTCGGTGGGTGAGGAAAAAGAACAGATGTACGGCGTCATGGAGGCCTGCGCCCCGCTGATGCCACAG
GACCATCCCCGCTACATCATGGGCATCGGCGCCCCCCAGGACCTGGTGGAGGCGGTCTGGCACGGCTACGACATGTTCGA
TTGCGTCATGCCGACCCGCAACGCCCGCAACGGCATGCTCTTCACCAGCCAGGGGCGGATCAACATCAAGGCCAAGATCT
TTGAAGAGGATTCCGGACCGCTGGACCCGGAGTGCGGCTGCCACGTCTGCAGGAACTACTCCCGCGCCTATCTGCGGCAC
CTCTACCGGGCGGGCGAAATCCTGGCCTCGAACCTGAACACCTATCACAACCTGTACTATTTCCTCGACCTGATGCGCCA
GATGCGCGAGGCGATCAAAAACGACAACCTGGCGGGATTCCGCCGCGAGTTTTACGCAAAGCAGCAGATACATCATAAAA
TTTAG

Upstream 100 bases:

>100_bases
TGACCCCCCCTGTGATGAAAAGGGGGAGAACGTGTCTCCAACCAGTCTATAGATCTTGTGACGTAAATAAAGCTGTACCA
TTGTCTTCCACGGGTGCTTA

Downstream 100 bases:

>100_bases
TAAGTTAAGGAGGTTTGTTCTATGTTAGGATTAGCATTTGCCATGTCGGGCCCGCCGGGCGGGGCAACGGGAGCCGGCGG
TGCAGCTGGCGCGTTTCAGC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 374; Mature: 373

Protein sequence:

>374_residues
MSTSLFTLLHRDASCGARLGSLNTLHGAIETPIFMPVGTNATVKAMTPDDLLAVNAQIILSNTYHLYLRPGHRLVEQLGG
LHSFMNWHRPILTDSGGFQVFSLGELRKISEEGVRFQSHLDGSYHFLTPELSIGIQEALGADIIMCFDECPPATAEYDYV
SRSLEMTTRWARRCKQAHRREGQQLFGIIQGGMHYDLRARSLEQIFEIGFDGYALGGLSVGEEKEQMYGVMEACAPLMPQ
DHPRYIMGIGAPQDLVEAVWHGYDMFDCVMPTRNARNGMLFTSQGRINIKAKIFEEDSGPLDPECGCHVCRNYSRAYLRH
LYRAGEILASNLNTYHNLYYFLDLMRQMREAIKNDNLAGFRREFYAKQQIHHKI

Sequences:

>Translated_374_residues
MSTSLFTLLHRDASCGARLGSLNTLHGAIETPIFMPVGTNATVKAMTPDDLLAVNAQIILSNTYHLYLRPGHRLVEQLGG
LHSFMNWHRPILTDSGGFQVFSLGELRKISEEGVRFQSHLDGSYHFLTPELSIGIQEALGADIIMCFDECPPATAEYDYV
SRSLEMTTRWARRCKQAHRREGQQLFGIIQGGMHYDLRARSLEQIFEIGFDGYALGGLSVGEEKEQMYGVMEACAPLMPQ
DHPRYIMGIGAPQDLVEAVWHGYDMFDCVMPTRNARNGMLFTSQGRINIKAKIFEEDSGPLDPECGCHVCRNYSRAYLRH
LYRAGEILASNLNTYHNLYYFLDLMRQMREAIKNDNLAGFRREFYAKQQIHHKI
>Mature_373_residues
STSLFTLLHRDASCGARLGSLNTLHGAIETPIFMPVGTNATVKAMTPDDLLAVNAQIILSNTYHLYLRPGHRLVEQLGGL
HSFMNWHRPILTDSGGFQVFSLGELRKISEEGVRFQSHLDGSYHFLTPELSIGIQEALGADIIMCFDECPPATAEYDYVS
RSLEMTTRWARRCKQAHRREGQQLFGIIQGGMHYDLRARSLEQIFEIGFDGYALGGLSVGEEKEQMYGVMEACAPLMPQD
HPRYIMGIGAPQDLVEAVWHGYDMFDCVMPTRNARNGMLFTSQGRINIKAKIFEEDSGPLDPECGCHVCRNYSRAYLRHL
YRAGEILASNLNTYHNLYYFLDLMRQMREAIKNDNLAGFRREFYAKQQIHHKI

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=349, Percent_Identity=46.4183381088825, Blast_Score=322, Evalue=3e-88,
Organism=Homo sapiens, GI13375872, Length=278, Percent_Identity=24.8201438848921, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI1786607, Length=354, Percent_Identity=55.0847457627119, Blast_Score=409, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI17544680, Length=349, Percent_Identity=44.4126074498567, Blast_Score=311, Evalue=4e-85,
Organism=Drosophila melanogaster, GI19920510, Length=352, Percent_Identity=46.3068181818182, Blast_Score=327, Evalue=6e-90,
Organism=Drosophila melanogaster, GI21355311, Length=236, Percent_Identity=24.1525423728814, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 42451; Mature: 42320

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSLFTLLHRDASCGARLGSLNTLHGAIETPIFMPVGTNATVKAMTPDDLLAVNAQIIL
CCCHHHHHHHCCCHHCHHCCCCHHHHHHHCCCEEEEECCCCEEEEECCCCHHEECEEEEE
SNTYHLYLRPGHRLVEQLGGLHSFMNWHRPILTDSGGFQVFSLGELRKISEEGVRFQSHL
ECCEEEEECCHHHHHHHHHHHHHHHHHCCCEEECCCCEEEEECHHHHHHHHHCCEEHHHC
DGSYHFLTPELSIGIQEALGADIIMCFDECPPATAEYDYVSRSLEMTTRWARRCKQAHRR
CCCEEEECCCHHCCHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EGQQLFGIIQGGMHYDLRARSLEQIFEIGFDGYALGGLSVGEEKEQMYGVMEACAPLMPQ
CHHHHHHHHHCCCEECHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCC
DHPRYIMGIGAPQDLVEAVWHGYDMFDCVMPTRNARNGMLFTSQGRINIKAKIFEEDSGP
CCCCEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCCCEEECCCCEEEEEEEEECCCCC
LDPECGCHVCRNYSRAYLRHLYRAGEILASNLNTYHNLYYFLDLMRQMREAIKNDNLAGF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
RREFYAKQQIHHKI
HHHHHHHHHHHHCC
>Mature Secondary Structure 
STSLFTLLHRDASCGARLGSLNTLHGAIETPIFMPVGTNATVKAMTPDDLLAVNAQIIL
CCHHHHHHHCCCHHCHHCCCCHHHHHHHCCCEEEEECCCCEEEEECCCCHHEECEEEEE
SNTYHLYLRPGHRLVEQLGGLHSFMNWHRPILTDSGGFQVFSLGELRKISEEGVRFQSHL
ECCEEEEECCHHHHHHHHHHHHHHHHHCCCEEECCCCEEEEECHHHHHHHHHCCEEHHHC
DGSYHFLTPELSIGIQEALGADIIMCFDECPPATAEYDYVSRSLEMTTRWARRCKQAHRR
CCCEEEECCCHHCCHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EGQQLFGIIQGGMHYDLRARSLEQIFEIGFDGYALGGLSVGEEKEQMYGVMEACAPLMPQ
CHHHHHHHHHCCCEECHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCC
DHPRYIMGIGAPQDLVEAVWHGYDMFDCVMPTRNARNGMLFTSQGRINIKAKIFEEDSGP
CCCCEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCCCEEECCCCEEEEEEEEECCCCC
LDPECGCHVCRNYSRAYLRHLYRAGEILASNLNTYHNLYYFLDLMRQMREAIKNDNLAGF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
RREFYAKQQIHHKI
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA