Click here to switch to the map view.

The map label for this gene is nicK [H]

Identifier: 118580176

GI number: 118580176

Start: 1888234

End: 1889271

Strand: Direct

Name: nicK [H]

Synonym: Ppro_1756

Alternate gene names: 118580176

Gene position: 1888234-1889271 (Clockwise)

Preceding gene: 118580175

Following gene: 118580177

Centisome position: 47.11

GC content: 51.64

Gene sequence:

>1038_bases
ATGCCCCCGTCTAGTAACACGGGGGCTAAAAATACATCCCTCGTTCCTTCTGAGAATCGGGCTTTGATTGATTGGCTCGC
CTGGACTGTCAAAGAGTTTGACCCCCACGCCGCTATTGCCAGTCTTGGCCTATCGTTCCTTCCGTTCCAGGCTTCCCCAG
GTGGTGGCATGGGCTACCGCTCAACCTTGCGCTGCGGTCATATTACCGTTTTTTTCGATGGTGCCGAAAATATGGGATGT
CATTTCGTTATGTCCGGTCAAGGGTGCCGTGAGTTTGAAGGGCGAGTCAAAAACTCGAAAAATCGAACTGTTGCGTGGTA
TCGCCTCATGCACTCGTTACAGGCTGCCGGTGCCAATATCACCCGCATTGACTTGGCAGTTGATACCGTGGATGGTTCGC
TGCCCCTTGAAAACGTCGAAAAGTCGGTTCGTGAACGTCGGGTTCAAAGCCGTTTCCGTGAGGCGATGATGTACGAGAAA
ATTGCTTTGACTGACACGGAAAAGCCCACAGGCAAAACGATCTATCTCGGGTCGTCCCAGGGCAAAATCAAAATTCGCTT
TTACGACAAGATGGCCGAGCGTGGCCTTTCCCCCGATCTTGGCTCCTGGGTTCGTGCCGAGGTTCAGTGCATGGCTGAAC
GCGCCGCTGCCGTAGTTATGCACCTGTGCAAAGGTGTTGAGTTCGGTGAGCTCGTAGTCTCCTGCCTGAATACCTATTAC
CGCCCCATTAATGCCGAATCCGTCAACAAGTCTCATTGTTCAACTCAGGACTGGTGGTCTGCATGGCTCACCACCACTGA
GAAAATCCGCCTCACATCCGCGAAAGCGGTCAAATACGTTACCGACAGCATGGAGTACGTCAAACGCCAGTACGGCGCAA
CCTTCGCCATGTTCAAAAAATATCTCGGTGTTGCTGCCTTCTCCGATTTTATGCATGACCTTGTTTCCACAGGTCGCGAC
AGAATGAGTCGCAAGCATACCGACATGATCGAAATAACGAATCTCTCCGAATTATTGCCCCCATACCATGCATTTTAA

Upstream 100 bases:

>100_bases
GTAACGCCCAAAGCAACCTACTCCCAAACGGAAAAATTAAGAGAGTTAAAGAAAAGTCTGTTGATACACGGCAACTCGAT
CTGTTTGAGGGTGCCGCCTG

Downstream 100 bases:

>100_bases
AAAGGTGTCAAAATGACAAACTCTAGCATAATCAGCAGGATACCGCACGAAGTTAAAGTGATTGATGAAGAAACCACCTC
TGTGACATTCATTCTTCCCA

Product: replication initiation factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MPPSSNTGAKNTSLVPSENRALIDWLAWTVKEFDPHAAIASLGLSFLPFQASPGGGMGYRSTLRCGHITVFFDGAENMGC
HFVMSGQGCREFEGRVKNSKNRTVAWYRLMHSLQAAGANITRIDLAVDTVDGSLPLENVEKSVRERRVQSRFREAMMYEK
IALTDTEKPTGKTIYLGSSQGKIKIRFYDKMAERGLSPDLGSWVRAEVQCMAERAAAVVMHLCKGVEFGELVVSCLNTYY
RPINAESVNKSHCSTQDWWSAWLTTTEKIRLTSAKAVKYVTDSMEYVKRQYGATFAMFKKYLGVAAFSDFMHDLVSTGRD
RMSRKHTDMIEITNLSELLPPYHAF

Sequences:

>Translated_345_residues
MPPSSNTGAKNTSLVPSENRALIDWLAWTVKEFDPHAAIASLGLSFLPFQASPGGGMGYRSTLRCGHITVFFDGAENMGC
HFVMSGQGCREFEGRVKNSKNRTVAWYRLMHSLQAAGANITRIDLAVDTVDGSLPLENVEKSVRERRVQSRFREAMMYEK
IALTDTEKPTGKTIYLGSSQGKIKIRFYDKMAERGLSPDLGSWVRAEVQCMAERAAAVVMHLCKGVEFGELVVSCLNTYY
RPINAESVNKSHCSTQDWWSAWLTTTEKIRLTSAKAVKYVTDSMEYVKRQYGATFAMFKKYLGVAAFSDFMHDLVSTGRD
RMSRKHTDMIEITNLSELLPPYHAF
>Mature_344_residues
PPSSNTGAKNTSLVPSENRALIDWLAWTVKEFDPHAAIASLGLSFLPFQASPGGGMGYRSTLRCGHITVFFDGAENMGCH
FVMSGQGCREFEGRVKNSKNRTVAWYRLMHSLQAAGANITRIDLAVDTVDGSLPLENVEKSVRERRVQSRFREAMMYEKI
ALTDTEKPTGKTIYLGSSQGKIKIRFYDKMAERGLSPDLGSWVRAEVQCMAERAAAVVMHLCKGVEFGELVVSCLNTYYR
PINAESVNKSHCSTQDWWSAWLTTTEKIRLTSAKAVKYVTDSMEYVKRQYGATFAMFKKYLGVAAFSDFMHDLVSTGRDR
MSRKHTDMIEITNLSELLPPYHAF

Specific function: Probable DNA relaxase involved in the transfer of the integrative and conjugative element ICEBs1. Required for the transfer of ICEBs1. Probably mediates conjugation of ICEBs1 by nicking at oriT on the conjugative element and facilitates the translocation

COG id: COG2946

COG function: function code L; Putative phage replication protein RstA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the plasmid replication initiation factor family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003491 [H]

Pfam domain/function: PF02486 Rep_trans [H]

EC number: NA

Molecular weight: Translated: 38710; Mature: 38579

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPSSNTGAKNTSLVPSENRALIDWLAWTVKEFDPHAAIASLGLSFLPFQASPGGGMGYR
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHCCHHHCCCCCCCCCCCCCH
STLRCGHITVFFDGAENMGCHFVMSGQGCREFEGRVKNSKNRTVAWYRLMHSLQAAGANI
HEEEECEEEEEEECCCCCCEEEEECCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCE
TRIDLAVDTVDGSLPLENVEKSVRERRVQSRFREAMMYEKIALTDTEKPTGKTIYLGSSQ
EEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCC
GKIKIRFYDKMAERGLSPDLGSWVRAEVQCMAERAAAVVMHLCKGVEFGELVVSCLNTYY
CEEEEEEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHC
RPINAESVNKSHCSTQDWWSAWLTTTEKIRLTSAKAVKYVTDSMEYVKRQYGATFAMFKK
CCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
YLGVAAFSDFMHDLVSTGRDRMSRKHTDMIEITNLSELLPPYHAF
HHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEECCHHHHCCCCCCC
>Mature Secondary Structure 
PPSSNTGAKNTSLVPSENRALIDWLAWTVKEFDPHAAIASLGLSFLPFQASPGGGMGYR
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHCCHHHCCCCCCCCCCCCCH
STLRCGHITVFFDGAENMGCHFVMSGQGCREFEGRVKNSKNRTVAWYRLMHSLQAAGANI
HEEEECEEEEEEECCCCCCEEEEECCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCE
TRIDLAVDTVDGSLPLENVEKSVRERRVQSRFREAMMYEKIALTDTEKPTGKTIYLGSSQ
EEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCC
GKIKIRFYDKMAERGLSPDLGSWVRAEVQCMAERAAAVVMHLCKGVEFGELVVSCLNTYY
CEEEEEEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHC
RPINAESVNKSHCSTQDWWSAWLTTTEKIRLTSAKAVKYVTDSMEYVKRQYGATFAMFKK
CCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
YLGVAAFSDFMHDLVSTGRDRMSRKHTDMIEITNLSELLPPYHAF
HHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEECCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]