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The map label for this gene is nadB [H]

Identifier: 118580156

GI number: 118580156

Start: 1866847

End: 1868442

Strand: Reverse

Name: nadB [H]

Synonym: Ppro_1736

Alternate gene names: 118580156

Gene position: 1868442-1866847 (Counterclockwise)

Preceding gene: 118580157

Following gene: 118580155

Centisome position: 46.62

GC content: 61.72

Gene sequence:

>1596_bases
ATGCGTATCGAAAGTGATTTTCTCGTGATCGGCAGCGGCATCGCCGGCCTCTCCTATGCCCTCCAGGCAGCAGATCATGG
CCGCGTTGCACTTGTCACCAAGTATGATGTTTCGGAATCAGCCACGAACTATGCTCAGGGGGGGATCGCCTCTGTCATCT
CGTCCGAGGATTCGTTCGACGCCCACATCAACGACACCATGGTTGCCGGAGCAGGTATCTGTCACGAGGACGTGGTACGC
ATGGTTGTTGAGGAAGGCCCCCAGACCATCAGGAACCTGATCGACTGGGGGGTCAAGTTCACCACCAAGGGTTCCAGCTA
CGACCTTACCCGCGAGGGGGGGCACAGCGCCCGGCGCATTCTGCACGCCGAGGACATCACCGGCCGCGAGATTCAGCGCG
CCCTGGTGGAGGCGGCCCGCAACCACAAAAACATCACCATCCATGAACACCACATCGCCATCGACCTGATCACCTCCGCC
AAGATCTGCAACGTGCCCCCCTGTGAAAACCGCTGTCTGGGCGCCTATGTGCTGGACATCAAGGGGAAGGTGGTCAAGAC
CTTCTCGTCCCAGATCACGCTTATGGCCAGCGGCGGGGCGGGCAAGGTCTACCTGTACACCTGCAACCCGGACATTGCCT
CGGGTGACGGCGTGGCCATGGCCTACCGGGCCGGGGCCACCATCGCCAACATGGAGTTCATGCAGTTCCACCCCACCACC
CTCTTCCACCCCCAGGGCAAATCGTTCCTGATCTCCGAGGCGGTGCGCGGCGAAGGGGCCATCCTGCGCCGGCGTGACGG
AACCGCATTCATGGAGAAGTACCACAAGCTCAAAGACCTGGCGCCCCGGGACATCGTGGCCCGCGCCATCGACAACGAGA
TGAAGATCAGCGGCGACGACTGCGTCTTTTTGGACATCACCCACGAGCCGGCCGACGTGGTGCGCAACCGTTTCCCCAAC
ATCTACCAGACCTGTCTGGAACTGGGTCTGGACATGACCAGTGAGTGGCTGCCGGTGGTACCGGCGGCCCATTACCTGTG
CGGCGGCGTGGCGGTCAACACCAACGGCGAGACCGATCTGAAGGCACTGTACGCCATCGGCGAGGTGACCTTCACCGGCC
TGCACGGCGCCAACCGGCTGGCCAGCAACTCGCTCCTCGAGGCGGCTGTCTACGCCAACCGCGCCTACCGGCACACGGTG
GAGGCGATCAAGCAGCTGCGCCAAGAGGCGGTGGAGATACCGCTCTGGAATTCGGGCACCGCCACCAACAGCGACGAGAT
GGTGGTTGTTTCCAATAACTGGGACGAGATCCGGCGCTTCATGTGGAACTACGTGGGCATCGTCCGCTCGGACAAGCGCC
TGGCCAGGGCCATGCGCCGCGTGGAACTGATCCAGAGCGAGATCGAGGAGTACTACTGGAACGTCATCATCACCTCGGAC
CTGATCGAACTGCGCAACATCGCCACCGTGGCCCAGCTGATCGTGGCCTGCGCCCAGATGCGCAAGGAGTCGCGCGGCCT
GCACTACACCATTGACCACCCTGAACGGGATGACGTGAATTGCAGACACGATACCTTCATCAGGAAAGAATTCTAA

Upstream 100 bases:

>100_bases
GATGCCTGGTACACAGGGTCCCTCTCTGCTTGACAAACCGGCAGCAGGAGACAATAATCCAGACGTTTTATCCACCTTAT
CCGCAAGGGGATCCCCTGAC

Downstream 100 bases:

>100_bases
ACGGAGAGCACTTTGGACATAGACGGGATCAGAAAACGTATTGACCTTCTGGACGACGTGCTGCTGCGTATCTTCAACGA
GCGCGCCCGCCTGGCCCTGG

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 531; Mature: 531

Protein sequence:

>531_residues
MRIESDFLVIGSGIAGLSYALQAADHGRVALVTKYDVSESATNYAQGGIASVISSEDSFDAHINDTMVAGAGICHEDVVR
MVVEEGPQTIRNLIDWGVKFTTKGSSYDLTREGGHSARRILHAEDITGREIQRALVEAARNHKNITIHEHHIAIDLITSA
KICNVPPCENRCLGAYVLDIKGKVVKTFSSQITLMASGGAGKVYLYTCNPDIASGDGVAMAYRAGATIANMEFMQFHPTT
LFHPQGKSFLISEAVRGEGAILRRRDGTAFMEKYHKLKDLAPRDIVARAIDNEMKISGDDCVFLDITHEPADVVRNRFPN
IYQTCLELGLDMTSEWLPVVPAAHYLCGGVAVNTNGETDLKALYAIGEVTFTGLHGANRLASNSLLEAAVYANRAYRHTV
EAIKQLRQEAVEIPLWNSGTATNSDEMVVVSNNWDEIRRFMWNYVGIVRSDKRLARAMRRVELIQSEIEEYYWNVIITSD
LIELRNIATVAQLIVACAQMRKESRGLHYTIDHPERDDVNCRHDTFIRKEF

Sequences:

>Translated_531_residues
MRIESDFLVIGSGIAGLSYALQAADHGRVALVTKYDVSESATNYAQGGIASVISSEDSFDAHINDTMVAGAGICHEDVVR
MVVEEGPQTIRNLIDWGVKFTTKGSSYDLTREGGHSARRILHAEDITGREIQRALVEAARNHKNITIHEHHIAIDLITSA
KICNVPPCENRCLGAYVLDIKGKVVKTFSSQITLMASGGAGKVYLYTCNPDIASGDGVAMAYRAGATIANMEFMQFHPTT
LFHPQGKSFLISEAVRGEGAILRRRDGTAFMEKYHKLKDLAPRDIVARAIDNEMKISGDDCVFLDITHEPADVVRNRFPN
IYQTCLELGLDMTSEWLPVVPAAHYLCGGVAVNTNGETDLKALYAIGEVTFTGLHGANRLASNSLLEAAVYANRAYRHTV
EAIKQLRQEAVEIPLWNSGTATNSDEMVVVSNNWDEIRRFMWNYVGIVRSDKRLARAMRRVELIQSEIEEYYWNVIITSD
LIELRNIATVAQLIVACAQMRKESRGLHYTIDHPERDDVNCRHDTFIRKEF
>Mature_531_residues
MRIESDFLVIGSGIAGLSYALQAADHGRVALVTKYDVSESATNYAQGGIASVISSEDSFDAHINDTMVAGAGICHEDVVR
MVVEEGPQTIRNLIDWGVKFTTKGSSYDLTREGGHSARRILHAEDITGREIQRALVEAARNHKNITIHEHHIAIDLITSA
KICNVPPCENRCLGAYVLDIKGKVVKTFSSQITLMASGGAGKVYLYTCNPDIASGDGVAMAYRAGATIANMEFMQFHPTT
LFHPQGKSFLISEAVRGEGAILRRRDGTAFMEKYHKLKDLAPRDIVARAIDNEMKISGDDCVFLDITHEPADVVRNRFPN
IYQTCLELGLDMTSEWLPVVPAAHYLCGGVAVNTNGETDLKALYAIGEVTFTGLHGANRLASNSLLEAAVYANRAYRHTV
EAIKQLRQEAVEIPLWNSGTATNSDEMVVVSNNWDEIRRFMWNYVGIVRSDKRLARAMRRVELIQSEIEEYYWNVIITSD
LIELRNIATVAQLIVACAQMRKESRGLHYTIDHPERDDVNCRHDTFIRKEF

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=532, Percent_Identity=29.1353383458647, Blast_Score=198, Evalue=8e-51,
Organism=Escherichia coli, GI1788928, Length=515, Percent_Identity=53.9805825242718, Blast_Score=562, Evalue=1e-161,
Organism=Escherichia coli, GI1790597, Length=532, Percent_Identity=31.203007518797, Blast_Score=231, Evalue=1e-61,
Organism=Escherichia coli, GI1786942, Length=561, Percent_Identity=31.5508021390374, Blast_Score=200, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17550100, Length=538, Percent_Identity=29.9256505576208, Blast_Score=201, Evalue=6e-52,
Organism=Caenorhabditis elegans, GI17505833, Length=534, Percent_Identity=29.4007490636704, Blast_Score=196, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6322701, Length=534, Percent_Identity=31.0861423220974, Blast_Score=212, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6322416, Length=544, Percent_Identity=29.4117647058824, Blast_Score=211, Evalue=2e-55,
Organism=Drosophila melanogaster, GI17137288, Length=540, Percent_Identity=28.8888888888889, Blast_Score=207, Evalue=2e-53,
Organism=Drosophila melanogaster, GI24655642, Length=540, Percent_Identity=28.8888888888889, Blast_Score=207, Evalue=2e-53,
Organism=Drosophila melanogaster, GI24655647, Length=540, Percent_Identity=28.8888888888889, Blast_Score=207, Evalue=2e-53,
Organism=Drosophila melanogaster, GI24663005, Length=535, Percent_Identity=29.3457943925234, Blast_Score=189, Evalue=3e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 59007; Mature: 59007

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIESDFLVIGSGIAGLSYALQAADHGRVALVTKYDVSESATNYAQGGIASVISSEDSFD
CCCCCCEEEEECCHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHCCCHHHHHCCCCCCC
AHINDTMVAGAGICHEDVVRMVVEEGPQTIRNLIDWGVKFTTKGSSYDLTREGGHSARRI
CCCCCEEEECCCCCHHHHHHHHHHCCHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHE
LHAEDITGREIQRALVEAARNHKNITIHEHHIAIDLITSAKICNVPPCENRCLGAYVLDI
EECCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEEEEEECCCCCCCCCCCCCCCEEEEEEC
KGKVVKTFSSQITLMASGGAGKVYLYTCNPDIASGDGVAMAYRAGATIANMEFMQFHPTT
CCHHEEECCCCEEEEEECCCCEEEEEECCCCCCCCCCEEEEEECCCEEECEEEEEECCCE
LFHPQGKSFLISEAVRGEGAILRRRDGTAFMEKYHKLKDLAPRDIVARAIDNEMKISGDD
EECCCCCCEEEHHHHCCCCCEEEECCCHHHHHHHHHHHCCCHHHHHHHHHCCCEEECCCC
CVFLDITHEPADVVRNRFPNIYQTCLELGLDMTSEWLPVVPAAHYLCGGVAVNTNGETDL
EEEEEECCCHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCEEECCCCCCHH
KALYAIGEVTFTGLHGANRLASNSLLEAAVYANRAYRHTVEAIKQLRQEAVEIPLWNSGT
HHHHHHHCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
ATNSDEMVVVSNNWDEIRRFMWNYVGIVRSDKRLARAMRRVELIQSEIEEYYWNVIITSD
CCCCCCEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEHH
LIELRNIATVAQLIVACAQMRKESRGLHYTIDHPERDDVNCRHDTFIRKEF
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEECCC
>Mature Secondary Structure
MRIESDFLVIGSGIAGLSYALQAADHGRVALVTKYDVSESATNYAQGGIASVISSEDSFD
CCCCCCEEEEECCHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHCCCHHHHHCCCCCCC
AHINDTMVAGAGICHEDVVRMVVEEGPQTIRNLIDWGVKFTTKGSSYDLTREGGHSARRI
CCCCCEEEECCCCCHHHHHHHHHHCCHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHE
LHAEDITGREIQRALVEAARNHKNITIHEHHIAIDLITSAKICNVPPCENRCLGAYVLDI
EECCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEEEEEECCCCCCCCCCCCCCCEEEEEEC
KGKVVKTFSSQITLMASGGAGKVYLYTCNPDIASGDGVAMAYRAGATIANMEFMQFHPTT
CCHHEEECCCCEEEEEECCCCEEEEEECCCCCCCCCCEEEEEECCCEEECEEEEEECCCE
LFHPQGKSFLISEAVRGEGAILRRRDGTAFMEKYHKLKDLAPRDIVARAIDNEMKISGDD
EECCCCCCEEEHHHHCCCCCEEEECCCHHHHHHHHHHHCCCHHHHHHHHHCCCEEECCCC
CVFLDITHEPADVVRNRFPNIYQTCLELGLDMTSEWLPVVPAAHYLCGGVAVNTNGETDL
EEEEEECCCHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCEEECCCCCCHH
KALYAIGEVTFTGLHGANRLASNSLLEAAVYANRAYRHTVEAIKQLRQEAVEIPLWNSGT
HHHHHHHCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
ATNSDEMVVVSNNWDEIRRFMWNYVGIVRSDKRLARAMRRVELIQSEIEEYYWNVIITSD
CCCCCCEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEHH
LIELRNIATVAQLIVACAQMRKESRGLHYTIDHPERDDVNCRHDTFIRKEF
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]