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The map label for this gene is glnA [H]

Identifier: 118580103

GI number: 118580103

Start: 1799084

End: 1800496

Strand: Direct

Name: glnA [H]

Synonym: Ppro_1681

Alternate gene names: 118580103

Gene position: 1799084-1800496 (Clockwise)

Preceding gene: 118580102

Following gene: 118580104

Centisome position: 44.89

GC content: 56.19

Gene sequence:

>1413_bases
ATGACTCCGAAACAAGTAGTAGAGTTTGCCAAAGAAAATGGCGCACTGATGGTTGATTACAAATTCATGGACTTTGTGGG
AACCTGGCAGCACGTGTCGGTGCCCATCACCGAGTTCGATGAGGATACCTTTGAGGAGGGGCAGGGTTTTGACGGTTCTT
CCATCCGTGGCTGGCAGCCGATCCACGCTTCCGACATGATCCTGCTCCCGGATCCGACCTCGGCCAAGATGGACCCCTTC
ATCGCCGTGCCGACCCTGTCGCTGATCTGCGACATCTTCGATCCCATCACCAAGGAAGACTACACCCGCGACCCGCGCAA
CATCGCCAAGAAGGCTGAGATGTACCTGAAGTCCACCGGCCTGGGCGATACGGCTTTCTTCGGTCCCGAGGCCGAATTCT
TCATCTTCGACGACGTACGCTTCGATTCCGGCCCCAACCAGTCCTTCTACGCCGTTGATTCCTCCGAGGGCGCCTGGAAC
ACCGGTCGCGAGGAATTTCCCAACCTGGGCTACAAACCGCGCTTCAAGGAAGGCTACTTCCCCTGCTCGCCGGTTGACTC
CCAGAACGACCTGCGCAACGAAATGGTGCAGGAACTCCAGAATGTTGGCATCCGGGTCGAGTGCCAACACCATGAAGTTG
CCACCGGCGGACAGGCCGAGATCGATATGCGCTTCTCTCCGCTGGTCGACATGGCCGACCAGTTGCAGTGGTTCAAGTAT
GTCATCAAGAACGTAGCCTATCGTAACAACAAGACCGTTACCTTCATGCCGAAACCGCTCTTCGGTGATAATGGTTCCGG
CATGCACTGTCACGTCTCAATCTGGAAGGACGGTGTCAACCTGTTTGCCGGTGATAAGTACGGTGGACTCTCCCAGATGG
CTCTCTGGTACATCGGTGGCATCATCAAACATGCCAAGGCTCTCTGCGCCATAACCAACCCGACCACCAACTCCTACAAG
CGCCTGGTGCCCGGTTTCGAAGCTCCGGTTAATATTGCCTATTCCAGCCGTAACCGTTCAGCGGCCATCCGTATCCCGAT
GATGTCCACCAACCCCAAGGCCAAGCGCGTCGAGTACCGTACTCCCGACCCCTCCTGCAACGGCTATCTGGCTTTTGCCG
CCCTGCTCATGGCCGGCCTTGACGGCATCGAGAACAAGATCGATCCGGGCCAGCCGCTGGACAAGGATATCTACGGTCTT
TCTCCTGAGGAACTCAAGAGTATCCCGTCCGCTCCGGGCAGCCTGGAAGAGGCGCTCAACTGCCTGAAGGAAGATCACGA
ATTCCTGCTCAAGGGCGATGTTTTTACCCCCGACGTCATCGAGAAGTGGATAGAGTACAAGATGGATGCTGAAGTCAACC
CGATTCGCATGCGTCCGACTCCGTTGGAGTTTTCCCTGTATTACGATATCTAA

Upstream 100 bases:

>100_bases
CTTTGTGACCCCCGTTGAGGCTGTTGTTCGTATCAGGACAGGAGAAACCGGAGAAGAGGCTCTGTAACAATTTTTATACC
CCCGAAAGGAGAAACGCACG

Downstream 100 bases:

>100_bases
ACAGCCCATTTTCATTGAAACGCGAAGGGCGGGAGCAATCCCGCCTTTTTTCGTATCTGAACGTCTGTACATGCAGCCGT
ATCACTCTCAACCTTCAGTA

Product: glutamine synthetase, type I

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MTPKQVVEFAKENGALMVDYKFMDFVGTWQHVSVPITEFDEDTFEEGQGFDGSSIRGWQPIHASDMILLPDPTSAKMDPF
IAVPTLSLICDIFDPITKEDYTRDPRNIAKKAEMYLKSTGLGDTAFFGPEAEFFIFDDVRFDSGPNQSFYAVDSSEGAWN
TGREEFPNLGYKPRFKEGYFPCSPVDSQNDLRNEMVQELQNVGIRVECQHHEVATGGQAEIDMRFSPLVDMADQLQWFKY
VIKNVAYRNNKTVTFMPKPLFGDNGSGMHCHVSIWKDGVNLFAGDKYGGLSQMALWYIGGIIKHAKALCAITNPTTNSYK
RLVPGFEAPVNIAYSSRNRSAAIRIPMMSTNPKAKRVEYRTPDPSCNGYLAFAALLMAGLDGIENKIDPGQPLDKDIYGL
SPEELKSIPSAPGSLEEALNCLKEDHEFLLKGDVFTPDVIEKWIEYKMDAEVNPIRMRPTPLEFSLYYDI

Sequences:

>Translated_470_residues
MTPKQVVEFAKENGALMVDYKFMDFVGTWQHVSVPITEFDEDTFEEGQGFDGSSIRGWQPIHASDMILLPDPTSAKMDPF
IAVPTLSLICDIFDPITKEDYTRDPRNIAKKAEMYLKSTGLGDTAFFGPEAEFFIFDDVRFDSGPNQSFYAVDSSEGAWN
TGREEFPNLGYKPRFKEGYFPCSPVDSQNDLRNEMVQELQNVGIRVECQHHEVATGGQAEIDMRFSPLVDMADQLQWFKY
VIKNVAYRNNKTVTFMPKPLFGDNGSGMHCHVSIWKDGVNLFAGDKYGGLSQMALWYIGGIIKHAKALCAITNPTTNSYK
RLVPGFEAPVNIAYSSRNRSAAIRIPMMSTNPKAKRVEYRTPDPSCNGYLAFAALLMAGLDGIENKIDPGQPLDKDIYGL
SPEELKSIPSAPGSLEEALNCLKEDHEFLLKGDVFTPDVIEKWIEYKMDAEVNPIRMRPTPLEFSLYYDI
>Mature_469_residues
TPKQVVEFAKENGALMVDYKFMDFVGTWQHVSVPITEFDEDTFEEGQGFDGSSIRGWQPIHASDMILLPDPTSAKMDPFI
AVPTLSLICDIFDPITKEDYTRDPRNIAKKAEMYLKSTGLGDTAFFGPEAEFFIFDDVRFDSGPNQSFYAVDSSEGAWNT
GREEFPNLGYKPRFKEGYFPCSPVDSQNDLRNEMVQELQNVGIRVECQHHEVATGGQAEIDMRFSPLVDMADQLQWFKYV
IKNVAYRNNKTVTFMPKPLFGDNGSGMHCHVSIWKDGVNLFAGDKYGGLSQMALWYIGGIIKHAKALCAITNPTTNSYKR
LVPGFEAPVNIAYSSRNRSAAIRIPMMSTNPKAKRVEYRTPDPSCNGYLAFAALLMAGLDGIENKIDPGQPLDKDIYGLS
PEELKSIPSAPGSLEEALNCLKEDHEFLLKGDVFTPDVIEKWIEYKMDAEVNPIRMRPTPLEFSLYYDI

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=471, Percent_Identity=56.687898089172, Blast_Score=555, Evalue=1e-159,
Organism=Escherichia coli, GI87081870, Length=187, Percent_Identity=29.9465240641711, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 52851; Mature: 52720

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPKQVVEFAKENGALMVDYKFMDFVGTWQHVSVPITEFDEDTFEEGQGFDGSSIRGWQP
CCHHHHHHHHHCCCCEEEEEEHHHHHCCCCEEEECHHHCCCHHHHHCCCCCCCCCCCCCC
IHASDMILLPDPTSAKMDPFIAVPTLSLICDIFDPITKEDYTRDPRNIAKKAEMYLKSTG
CCCCCEEEECCCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCC
LGDTAFFGPEAEFFIFDDVRFDSGPNQSFYAVDSSEGAWNTGREEFPNLGYKPRFKEGYF
CCCCEEECCCCCEEEEEEEEECCCCCCCEEEEECCCCCCCCCHHHCCCCCCCCCCCCCCC
PCSPVDSQNDLRNEMVQELQNVGIRVECQHHEVATGGQAEIDMRFSPLVDMADQLQWFKY
CCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHH
VIKNVAYRNNKTVTFMPKPLFGDNGSGMHCHVSIWKDGVNLFAGDKYGGLSQMALWYIGG
HHHHHHCCCCCEEEEECCCCCCCCCCCEEEEEEEEECCCEEEECCCCCCHHHHHHHHHHH
IIKHAKALCAITNPTTNSYKRLVPGFEAPVNIAYSSRNRSAAIRIPMMSTNPKAKRVEYR
HHHHHHHEEEECCCCCCHHHHHCCCCCCCEEEEEECCCCCEEEEEEEECCCCCCCEEEEC
TPDPSCNGYLAFAALLMAGLDGIENKIDPGQPLDKDIYGLSPEELKSIPSAPGSLEEALN
CCCCCCCHHHHHHHHHHHCCCHHHCCCCCCCCCCCHHCCCCHHHHHHCCCCCCHHHHHHH
CLKEDHEFLLKGDVFTPDVIEKWIEYKMDAEVNPIRMRPTPLEFSLYYDI
HHHHCCCEEEECCCCCHHHHHHHHHHCCCCCCCCEEECCCCEEEEEEECC
>Mature Secondary Structure 
TPKQVVEFAKENGALMVDYKFMDFVGTWQHVSVPITEFDEDTFEEGQGFDGSSIRGWQP
CHHHHHHHHHCCCCEEEEEEHHHHHCCCCEEEECHHHCCCHHHHHCCCCCCCCCCCCCC
IHASDMILLPDPTSAKMDPFIAVPTLSLICDIFDPITKEDYTRDPRNIAKKAEMYLKSTG
CCCCCEEEECCCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCC
LGDTAFFGPEAEFFIFDDVRFDSGPNQSFYAVDSSEGAWNTGREEFPNLGYKPRFKEGYF
CCCCEEECCCCCEEEEEEEEECCCCCCCEEEEECCCCCCCCCHHHCCCCCCCCCCCCCCC
PCSPVDSQNDLRNEMVQELQNVGIRVECQHHEVATGGQAEIDMRFSPLVDMADQLQWFKY
CCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHH
VIKNVAYRNNKTVTFMPKPLFGDNGSGMHCHVSIWKDGVNLFAGDKYGGLSQMALWYIGG
HHHHHHCCCCCEEEEECCCCCCCCCCCEEEEEEEEECCCEEEECCCCCCHHHHHHHHHHH
IIKHAKALCAITNPTTNSYKRLVPGFEAPVNIAYSSRNRSAAIRIPMMSTNPKAKRVEYR
HHHHHHHEEEECCCCCCHHHHHCCCCCCCEEEEEECCCCCEEEEEEEECCCCCCCEEEEC
TPDPSCNGYLAFAALLMAGLDGIENKIDPGQPLDKDIYGLSPEELKSIPSAPGSLEEALN
CCCCCCCHHHHHHHHHHHCCCHHHCCCCCCCCCCCHHCCCCHHHHHHCCCCCCHHHHHHH
CLKEDHEFLLKGDVFTPDVIEKWIEYKMDAEVNPIRMRPTPLEFSLYYDI
HHHHCCCEEEECCCCCHHHHHHHHHHCCCCCCCCEEECCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1362348 [H]