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The map label for this gene is livJ [C]

Identifier: 118580093

GI number: 118580093

Start: 1790477

End: 1791616

Strand: Direct

Name: livJ [C]

Synonym: Ppro_1671

Alternate gene names: 118580093

Gene position: 1790477-1791616 (Clockwise)

Preceding gene: 118580092

Following gene: 118580094

Centisome position: 44.67

GC content: 62.54

Gene sequence:

>1140_bases
GTGAAAAAAGTTGCACTGCTCTGTAGCCTCATCTGCATCACACTGCTGACCGCTTCGGCGGCCTTTGCCGCTCCCTCCCT
CAAGATCGGCGGCCTGTTTGCCGTGACCGGTCCGGCCGCCTTCCTGGGGGAACCGGAGCGTAACACCGCCATGATGGTGG
TGTCCGAGATCAACCGGGCAGGGGGCATCCGGGGACAAAAGCTTGAGCTCGTTGTCTACGATACGGCCGGGGATGCCACC
AAGGCGGTGCAGCTGGCCAACAAGCTGATCAAGAGCGACAGGGTCATGGCCATCATCGGGCCCAGCACTACCGGTGAGTC
CATGGCGGTCATACCGGTGGCCGAGCGCGGCAGGGTGCCGTTGATCTCCTGCGCCGCCGGGATAAAGATCACCGAACCGC
TGAAAAAGTGGGTCTTCAAGACGGCGCAGAACGACAGCCTGGCCGTTATCAGGATCTACGAACATCTCAAGAAACAGAGG
CTGCAGCGGGTGGCGCTGCTCACCGTTTCCGACGGTTTCGGGGCATCGGGCCGCGAACAGCTCAAGGCCCAGGCAGCCCG
CTATGGCATCAGGATCGTCGCCGACGACACCTACGGCCCCAAGGACACGGACATGACCTCCCAGTTGACCAAGATCCGCG
GCCTCAAGCCGGATGCCATCATCTGCTGGGGCACCAACCCCGGCCCGGCGGTGATCACCAAAAACGCCCGCCAGCTGGGG
ATAAAAACTCCGCTGTTCATGAGCCATGGCGTTTCCTCCAAGAAGTACATCCAACTGGCCGGAAAGGCTGCCGAAGGGGT
CAAGCTCCCCTCGGGCAGGGTGATCGTGGCTGACCAGCTTCCCGCCTCTGATCCCCAGAAACGCTCGCTGATAGCTTTCG
TCAAGGGGTATGAGAGATACTACAAGGCCGAGGGTGACCACTTCGGCGGCCATGCCTGGGATGCGGTCATGCTGCTCAAG
GGCGCCTTCGAGCGCGGCGCAACAACCCCGGCGGCGGTCCGCGACCAGCTGGAAAATACGCGCCAGTTCCACGGCATCGG
CGGCAGCTTCACCTTTACCTCCCGGGATCATGCCGGCCTGGGCAAGGATGCCTTCGTGCTGGTGGAGGTCAGGAAGGGCG
ACTGGGCGATCGTGAAGTAG

Upstream 100 bases:

>100_bases
TTTTTCCGTGTTTTTATGCTCTTTATTGGGACTGGAACCATACGTCATTACATGGAAATTGCGGTCTAATTGTTCGATAT
TACGAGATTGTGGAGGGACT

Downstream 100 bases:

>100_bases
GAAACGTCCGGACGGATTCTACGTAAACTATCCTCCCTCCCCATGCAGGGGAGGGAGTTTCTTTGTGAGAAACGGAACAT
CAATGCAGTTTGACCAGATC

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MKKVALLCSLICITLLTASAAFAAPSLKIGGLFAVTGPAAFLGEPERNTAMMVVSEINRAGGIRGQKLELVVYDTAGDAT
KAVQLANKLIKSDRVMAIIGPSTTGESMAVIPVAERGRVPLISCAAGIKITEPLKKWVFKTAQNDSLAVIRIYEHLKKQR
LQRVALLTVSDGFGASGREQLKAQAARYGIRIVADDTYGPKDTDMTSQLTKIRGLKPDAIICWGTNPGPAVITKNARQLG
IKTPLFMSHGVSSKKYIQLAGKAAEGVKLPSGRVIVADQLPASDPQKRSLIAFVKGYERYYKAEGDHFGGHAWDAVMLLK
GAFERGATTPAAVRDQLENTRQFHGIGGSFTFTSRDHAGLGKDAFVLVEVRKGDWAIVK

Sequences:

>Translated_379_residues
MKKVALLCSLICITLLTASAAFAAPSLKIGGLFAVTGPAAFLGEPERNTAMMVVSEINRAGGIRGQKLELVVYDTAGDAT
KAVQLANKLIKSDRVMAIIGPSTTGESMAVIPVAERGRVPLISCAAGIKITEPLKKWVFKTAQNDSLAVIRIYEHLKKQR
LQRVALLTVSDGFGASGREQLKAQAARYGIRIVADDTYGPKDTDMTSQLTKIRGLKPDAIICWGTNPGPAVITKNARQLG
IKTPLFMSHGVSSKKYIQLAGKAAEGVKLPSGRVIVADQLPASDPQKRSLIAFVKGYERYYKAEGDHFGGHAWDAVMLLK
GAFERGATTPAAVRDQLENTRQFHGIGGSFTFTSRDHAGLGKDAFVLVEVRKGDWAIVK
>Mature_379_residues
MKKVALLCSLICITLLTASAAFAAPSLKIGGLFAVTGPAAFLGEPERNTAMMVVSEINRAGGIRGQKLELVVYDTAGDAT
KAVQLANKLIKSDRVMAIIGPSTTGESMAVIPVAERGRVPLISCAAGIKITEPLKKWVFKTAQNDSLAVIRIYEHLKKQR
LQRVALLTVSDGFGASGREQLKAQAARYGIRIVADDTYGPKDTDMTSQLTKIRGLKPDAIICWGTNPGPAVITKNARQLG
IKTPLFMSHGVSSKKYIQLAGKAAEGVKLPSGRVIVADQLPASDPQKRSLIAFVKGYERYYKAEGDHFGGHAWDAVMLLK
GAFERGATTPAAVRDQLENTRQFHGIGGSFTFTSRDHAGLGKDAFVLVEVRKGDWAIVK

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=287, Percent_Identity=26.8292682926829, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1789867, Length=333, Percent_Identity=26.7267267267267, Blast_Score=82, Evalue=6e-17,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 40674; Mature: 40674

Theoretical pI: Translated: 10.40; Mature: 10.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVALLCSLICITLLTASAAFAAPSLKIGGLFAVTGPAAFLGEPERNTAMMVVSEINRA
CCHHHHHHHHHHHHHHHHHHHHCCCCEEECEEEEECCCHHHCCCCCCCCEEEEEEHHHHH
GGIRGQKLELVVYDTAGDATKAVQLANKLIKSDRVMAIIGPSTTGESMAVIPVAERGRVP
CCCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECCCCCCC
LISCAAGIKITEPLKKWVFKTAQNDSLAVIRIYEHLKKQRLQRVALLTVSDGFGASGREQ
EEEECCCCEEHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHEEEEEEEECCCCCCCHHH
LKAQAARYGIRIVADDTYGPKDTDMTSQLTKIRGLKPDAIICWGTNPGPAVITKNARQLG
HHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCEEEECCCHHCC
IKTPLFMSHGVSSKKYIQLAGKAAEGVKLPSGRVIVADQLPASDPQKRSLIAFVKGYERY
CCCCHHHHCCCCCCEEEEEECCCCCCEECCCCCEEEECCCCCCCCHHHHHHHHHHHHHHH
YKAEGDHFGGHAWDAVMLLKGAFERGATTPAAVRDQLENTRQFHGIGGSFTFTSRDHAGL
HHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCC
GKDAFVLVEVRKGDWAIVK
CCCEEEEEEEECCCEEEEC
>Mature Secondary Structure
MKKVALLCSLICITLLTASAAFAAPSLKIGGLFAVTGPAAFLGEPERNTAMMVVSEINRA
CCHHHHHHHHHHHHHHHHHHHHCCCCEEECEEEEECCCHHHCCCCCCCCEEEEEEHHHHH
GGIRGQKLELVVYDTAGDATKAVQLANKLIKSDRVMAIIGPSTTGESMAVIPVAERGRVP
CCCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECCCCCCC
LISCAAGIKITEPLKKWVFKTAQNDSLAVIRIYEHLKKQRLQRVALLTVSDGFGASGREQ
EEEECCCCEEHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHEEEEEEEECCCCCCCHHH
LKAQAARYGIRIVADDTYGPKDTDMTSQLTKIRGLKPDAIICWGTNPGPAVITKNARQLG
HHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCEEEECCCHHCC
IKTPLFMSHGVSSKKYIQLAGKAAEGVKLPSGRVIVADQLPASDPQKRSLIAFVKGYERY
CCCCHHHHCCCCCCEEEEEECCCCCCEECCCCCEEEECCCCCCCCHHHHHHHHHHHHHHH
YKAEGDHFGGHAWDAVMLLKGAFERGATTPAAVRDQLENTRQFHGIGGSFTFTSRDHAGL
HHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCC
GKDAFVLVEVRKGDWAIVK
CCCEEEEEEEECCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]