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The map label for this gene is metG [H]

Identifier: 118580084

GI number: 118580084

Start: 1780751

End: 1782271

Strand: Reverse

Name: metG [H]

Synonym: Ppro_1662

Alternate gene names: 118580084

Gene position: 1782271-1780751 (Counterclockwise)

Preceding gene: 118580085

Following gene: 118580076

Centisome position: 44.47

GC content: 62.13

Gene sequence:

>1521_bases
ATGAAACCTTCATTCTACGTCACCACCCCCATATACTACGTCAACGACGTGCCGCACATCGGCCATGCCTACACCACCGT
GGCAGCAGACGTGCTGGCGCGCTACAAGCGGCTGATGGGGTATGACGTCTATTTCCTGACCGGCAGCGACGAGCATGGCC
AGAAAGTGGAAAAGGCGGCCACCACCGCCGGCGAGACGCCGCTGGAACTGGCGGACCGGGTCGTCAAGCGCTATCAGGCG
CTCTGGGAACGCCTGGGCATATCCAACAACGACTTCATCAGGACCACCCAGGAGCGCCACAAGAAAGGGGTTTCCCAGCT
CTTCTCCCTGATCATGGAAAGGGGGGACATCTATCTGGGGGAATACGAGGACTGGTACTGTACTCCCTGCGAGACCTTCT
GGCCGGAAACCCAGCTGATTGAGGACCGCTGCCCGGACTGCAACCGGCCGGTGGAAAAGCTTAAAGAAGAGTCATACTTC
TTCCGCATGAGCAAGTACCAGGAGCAGCTCCTGGCCCACATCGAGGCCAACCCGGACTTCATCCAGCCCAAGAGCAAGCG
CAACGAGATCATCTCCTTCGTCAAAGAGGGGCTGCGCGACCTGTCGATCTCCCGCACCACCTTCAGCTGGGGCATCCCGG
TGCCGGGCAATGACAAGCACATCGTCTACGTCTGGTTCGACGCCCTGACCAACTACCTCACCGCCCTGGGATACCCCGAC
AGCACCGCCGAGTACGGACGGTTCTGGCCAGCCAACGTACACCTGATCGGCAAGGATATCCTGCGCTTCCACACCGTCTA
CTGGCCCACCTTCCTGATGGCGGCCGGCCTGCCCCTGCCGGAGAAGGTCTTCGCCCACGGCTGGTGGACGGTGGAGGGGC
AGAAGATGAGCAAGAGCCTGCAGAACGTGGTGGAGCCCAACATGCTGATCGACAAGTACGGCGTGGACGTGGTCAGGTAC
TTCCTGATGCGCGAGGTTCCCTTCGGCCTGGACGGCGACTTCTCCCACACCGCCCTGATCCAGCGGCTCAACTCGGACCT
GGCCAACGACCTGGGCAACCTGCTCTCCCGCTCCACCGCCATGGCGGTCAAGTATTTTGGCGGCATCCTGCCGGCTGCGA
CCGAATTGACCGAGATCGACCACTCCCTTCAGGAGAAGACAAGCGCCATGATCGCCGCGGTGGACAAATTCATGGATGAA
CTGGCCTTCAGCAAGGCGCTCCAGGCGATCTGGGAGGTCATCTCGGCCGGCAACAAATACATCGACGACTGCGCCCCCTG
GGTCCTGGCCAAGGATCCGGCTCAAAAGGACCGCCTGGCCACGGTCATGTACTGCCTGCTGGAATCCCAGCGCATCGCCC
ACTGCATCCTGTCGGCCTTCCTGCCCGCCACGACAGCCAAGGCGCTGGCCTCCCTGGGGTGGGACGGCGAGATTTCCCGG
GAGTCCCTGGCCTGGGGCGGTTTGCAAGAGGGGACCACGATCACCAAGGCCGAGGCGCTCTTCCCCCGCTACGAGGCCTG
A

Upstream 100 bases:

>100_bases
CGGAGAAGGCCACGGACACCCCCAAGGAGTAGGCACCACAACGTTGTACGGCGCGGAGCAGGGGCGGCAACCAACTACCA
CTAACCACCAGGAGCAGCAT

Downstream 100 bases:

>100_bases
TAATAGAACGGGGCGAACCATAAACTTGGTTCGCCCCGTTTCCGTTCCTGTATCAATCCTATCGCTTCAGTTGCTGTCAT
TGACGATGGAGAGAAAGAAA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MKPSFYVTTPIYYVNDVPHIGHAYTTVAADVLARYKRLMGYDVYFLTGSDEHGQKVEKAATTAGETPLELADRVVKRYQA
LWERLGISNNDFIRTTQERHKKGVSQLFSLIMERGDIYLGEYEDWYCTPCETFWPETQLIEDRCPDCNRPVEKLKEESYF
FRMSKYQEQLLAHIEANPDFIQPKSKRNEIISFVKEGLRDLSISRTTFSWGIPVPGNDKHIVYVWFDALTNYLTALGYPD
STAEYGRFWPANVHLIGKDILRFHTVYWPTFLMAAGLPLPEKVFAHGWWTVEGQKMSKSLQNVVEPNMLIDKYGVDVVRY
FLMREVPFGLDGDFSHTALIQRLNSDLANDLGNLLSRSTAMAVKYFGGILPAATELTEIDHSLQEKTSAMIAAVDKFMDE
LAFSKALQAIWEVISAGNKYIDDCAPWVLAKDPAQKDRLATVMYCLLESQRIAHCILSAFLPATTAKALASLGWDGEISR
ESLAWGGLQEGTTITKAEALFPRYEA

Sequences:

>Translated_506_residues
MKPSFYVTTPIYYVNDVPHIGHAYTTVAADVLARYKRLMGYDVYFLTGSDEHGQKVEKAATTAGETPLELADRVVKRYQA
LWERLGISNNDFIRTTQERHKKGVSQLFSLIMERGDIYLGEYEDWYCTPCETFWPETQLIEDRCPDCNRPVEKLKEESYF
FRMSKYQEQLLAHIEANPDFIQPKSKRNEIISFVKEGLRDLSISRTTFSWGIPVPGNDKHIVYVWFDALTNYLTALGYPD
STAEYGRFWPANVHLIGKDILRFHTVYWPTFLMAAGLPLPEKVFAHGWWTVEGQKMSKSLQNVVEPNMLIDKYGVDVVRY
FLMREVPFGLDGDFSHTALIQRLNSDLANDLGNLLSRSTAMAVKYFGGILPAATELTEIDHSLQEKTSAMIAAVDKFMDE
LAFSKALQAIWEVISAGNKYIDDCAPWVLAKDPAQKDRLATVMYCLLESQRIAHCILSAFLPATTAKALASLGWDGEISR
ESLAWGGLQEGTTITKAEALFPRYEA
>Mature_506_residues
MKPSFYVTTPIYYVNDVPHIGHAYTTVAADVLARYKRLMGYDVYFLTGSDEHGQKVEKAATTAGETPLELADRVVKRYQA
LWERLGISNNDFIRTTQERHKKGVSQLFSLIMERGDIYLGEYEDWYCTPCETFWPETQLIEDRCPDCNRPVEKLKEESYF
FRMSKYQEQLLAHIEANPDFIQPKSKRNEIISFVKEGLRDLSISRTTFSWGIPVPGNDKHIVYVWFDALTNYLTALGYPD
STAEYGRFWPANVHLIGKDILRFHTVYWPTFLMAAGLPLPEKVFAHGWWTVEGQKMSKSLQNVVEPNMLIDKYGVDVVRY
FLMREVPFGLDGDFSHTALIQRLNSDLANDLGNLLSRSTAMAVKYFGGILPAATELTEIDHSLQEKTSAMIAAVDKFMDE
LAFSKALQAIWEVISAGNKYIDDCAPWVLAKDPAQKDRLATVMYCLLESQRIAHCILSAFLPATTAKALASLGWDGEISR
ESLAWGGLQEGTTITKAEALFPRYEA

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=497, Percent_Identity=40.0402414486922, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI14043022, Length=513, Percent_Identity=27.4853801169591, Blast_Score=174, Evalue=3e-43,
Organism=Escherichia coli, GI1788432, Length=542, Percent_Identity=25.830258302583, Blast_Score=191, Evalue=9e-50,
Organism=Caenorhabditis elegans, GI212645096, Length=491, Percent_Identity=35.6415478615071, Blast_Score=298, Evalue=4e-81,
Organism=Caenorhabditis elegans, GI71992781, Length=380, Percent_Identity=33.1578947368421, Blast_Score=210, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI17540794, Length=521, Percent_Identity=26.4875239923225, Blast_Score=165, Evalue=7e-41,
Organism=Saccharomyces cerevisiae, GI6321610, Length=514, Percent_Identity=34.2412451361868, Blast_Score=301, Evalue=1e-82,
Organism=Saccharomyces cerevisiae, GI6321704, Length=545, Percent_Identity=24.954128440367, Blast_Score=135, Evalue=1e-32,
Organism=Drosophila melanogaster, GI21358483, Length=491, Percent_Identity=37.2708757637475, Blast_Score=323, Evalue=2e-88,
Organism=Drosophila melanogaster, GI24655311, Length=514, Percent_Identity=28.2101167315175, Blast_Score=175, Evalue=6e-44,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 57468; Mature: 57468

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPSFYVTTPIYYVNDVPHIGHAYTTVAADVLARYKRLMGYDVYFLTGSDEHGQKVEKAA
CCCCEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHH
TTAGETPLELADRVVKRYQALWERLGISNNDFIRTTQERHKKGVSQLFSLIMERGDIYLG
HCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEECHHHHHHHHHHHHHHHHHHCCCEEEC
EYEDWYCTPCETFWPETQLIEDRCPDCNRPVEKLKEESYFFRMSKYQEQLLAHIEANPDF
CCCCCEECCCHHHCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
IQPKSKRNEIISFVKEGLRDLSISRTTFSWGIPVPGNDKHIVYVWFDALTNYLTALGYPD
CCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEEEHHHHHHHHHHCCCCC
STAEYGRFWPANVHLIGKDILRFHTVYWPTFLMAAGLPLPEKVFAHGWWTVEGQKMSKSL
CCHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCEEECHHHHHHHH
QNVVEPNMLIDKYGVDVVRYFLMREVPFGLDGDFSHTALIQRLNSDLANDLGNLLSRSTA
HHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MAVKYFGGILPAATELTEIDHSLQEKTSAMIAAVDKFMDELAFSKALQAIWEVISAGNKY
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
IDDCAPWVLAKDPAQKDRLATVMYCLLESQRIAHCILSAFLPATTAKALASLGWDGEISR
HHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
ESLAWGGLQEGTTITKAEALFPRYEA
HHHHCCCCCCCCCCHHHHHHCCCCCC
>Mature Secondary Structure
MKPSFYVTTPIYYVNDVPHIGHAYTTVAADVLARYKRLMGYDVYFLTGSDEHGQKVEKAA
CCCCEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHH
TTAGETPLELADRVVKRYQALWERLGISNNDFIRTTQERHKKGVSQLFSLIMERGDIYLG
HCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEECHHHHHHHHHHHHHHHHHHCCCEEEC
EYEDWYCTPCETFWPETQLIEDRCPDCNRPVEKLKEESYFFRMSKYQEQLLAHIEANPDF
CCCCCEECCCHHHCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
IQPKSKRNEIISFVKEGLRDLSISRTTFSWGIPVPGNDKHIVYVWFDALTNYLTALGYPD
CCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEEEHHHHHHHHHHCCCCC
STAEYGRFWPANVHLIGKDILRFHTVYWPTFLMAAGLPLPEKVFAHGWWTVEGQKMSKSL
CCHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCEEECHHHHHHHH
QNVVEPNMLIDKYGVDVVRYFLMREVPFGLDGDFSHTALIQRLNSDLANDLGNLLSRSTA
HHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MAVKYFGGILPAATELTEIDHSLQEKTSAMIAAVDKFMDELAFSKALQAIWEVISAGNKY
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
IDDCAPWVLAKDPAQKDRLATVMYCLLESQRIAHCILSAFLPATTAKALASLGWDGEISR
HHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
ESLAWGGLQEGTTITKAEALFPRYEA
HHHHCCCCCCCCCCHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]