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The map label for this gene is pfp [H]

Identifier: 118580066

GI number: 118580066

Start: 1763563

End: 1764852

Strand: Reverse

Name: pfp [H]

Synonym: Ppro_1644

Alternate gene names: 118580066

Gene position: 1764852-1763563 (Counterclockwise)

Preceding gene: 118580067

Following gene: 118580065

Centisome position: 44.03

GC content: 64.34

Gene sequence:

>1290_bases
ATGAACCACCAGGAACCGGATTTCAACGTGGAACGTCTGGGCGAGTGCCGCTTCCCCTCTCCCATGCGGGGGGTACGCTT
CACCACCGACGGAGAACGTATCCTCTACCACTCCCGCTACCAGGAAATGCGTCCCTTCATCGACAGCGGCAGGGAGCCCC
CTTCCATGGAGATGGGCGGTCCGCGGGAACGGATCTTCTTCGACCCGTCCACCATCGCCTGCGGCATCGTCACCTGCGGC
GGCCTCTGCCCCGGAACCAACGACGTGATCCGCGCCCTGACCTTGAGCCTCTACCACCACTACGGCGTGCGGCGGGTCTA
CGGTTTCCGCTACGGCTTCGAGGGTGTGGTGAAGCGCCACGGCCACTCCCCCCTGCTGCTCACCCCCGAGGTGGTGGACC
GTATCGGTGAGCTGGGGGGGACGATCCTGGCTTCCTCCCGCGGCCCCCAGGCGCCGTCTGAAATGGTCGACTACCTGGAA
GAACTGGGCATAGCCATCCTCTTCACCATCGGCGGCGACGGAACCCTCAAAGGGGCTGCCAAGATCGCCGAAGAGGCGGC
CCGGCGGGGCAGGCCCATCAGCGTGATCGGCATACCCAAGACCATCGACAACGACGTATCCTTCCTGCAAAACACCTTCG
GTTTCGAAACCGCCGTGGACGAGGCCCACCGGGCCATCTACGCCGCCCACACCGAAGCCACCGGGGCACGCAACGGCATT
GGCCTGGTGAAGCTGATGGGACGGGACTCCGGCTTCATTGCCGCCTACTCCACCCTGGTCAACAGCCAGGTCAACTTCTG
CCTTGTTCCCGAGGTTCCCTTCACCCTGGCCGGGTTCCTGAAGAGCCTGGAGGAACGTCTGGCCCTGCGCGGCCATGCGG
TGATTGCCGTTGCCGAGGGAGCGGGCCAGGAGCTGCTGCCGGCCGCCACGGAGAGTGACGCATCGGGTAACGTCAAACTG
GGCGACATCGGCACCTTTCTGCGGGACGCCATCAACGGACACTTCCGCGCCATCGGCACGGAGATCACCCTCAAGTACAT
CGACCCAAGCTACATCATCCGCAGCCAGCCGGCCAACTCCCACGATTCGGCTTTCTGCCTGCTGCTGGGACACAACGCCG
TTCATGCCGGCATGTGCGGCAGGACCTGCATGGTTGTGGGGAACTGGAACCAGCAGTTCACCCATGTCCCCATCAGGCTC
GCAACCCACCAGAGGAAGAAGATCGACCCCAAGGGGCGACTCTGGAGCAGCGTCCTGGCATCCACCGGCCAGTCCCGCGA
GATAGACTGA

Upstream 100 bases:

>100_bases
AGCGGATCCTGAGACAAGCGCGGTGAGGCACCGCAGGTGCCCGGCCGGGTAAGTCCAAAACACCAGCAAAAACAAAGCTA
TCACACCCATAGGAGGAACA

Downstream 100 bases:

>100_bases
GCGCCAGACTGACAGCGGAAGGAAACGATCATGTTTCAACGAGTGCTTCTGGTTGATGCGTCCACCGGTTTTTACCGGAC
GAGAAAATACGGCTTCGAGC

Product: diphosphate--fructose-6-phosphate 1-phosphotransferase

Products: NA

Alternate protein names: 6-phosphofructokinase, pyrophosphate dependent; PPi-dependent phosphofructokinase; PPi-PFK; Pyrophosphate-dependent 6-phosphofructose-1-kinase [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MNHQEPDFNVERLGECRFPSPMRGVRFTTDGERILYHSRYQEMRPFIDSGREPPSMEMGGPRERIFFDPSTIACGIVTCG
GLCPGTNDVIRALTLSLYHHYGVRRVYGFRYGFEGVVKRHGHSPLLLTPEVVDRIGELGGTILASSRGPQAPSEMVDYLE
ELGIAILFTIGGDGTLKGAAKIAEEAARRGRPISVIGIPKTIDNDVSFLQNTFGFETAVDEAHRAIYAAHTEATGARNGI
GLVKLMGRDSGFIAAYSTLVNSQVNFCLVPEVPFTLAGFLKSLEERLALRGHAVIAVAEGAGQELLPAATESDASGNVKL
GDIGTFLRDAINGHFRAIGTEITLKYIDPSYIIRSQPANSHDSAFCLLLGHNAVHAGMCGRTCMVVGNWNQQFTHVPIRL
ATHQRKKIDPKGRLWSSVLASTGQSREID

Sequences:

>Translated_429_residues
MNHQEPDFNVERLGECRFPSPMRGVRFTTDGERILYHSRYQEMRPFIDSGREPPSMEMGGPRERIFFDPSTIACGIVTCG
GLCPGTNDVIRALTLSLYHHYGVRRVYGFRYGFEGVVKRHGHSPLLLTPEVVDRIGELGGTILASSRGPQAPSEMVDYLE
ELGIAILFTIGGDGTLKGAAKIAEEAARRGRPISVIGIPKTIDNDVSFLQNTFGFETAVDEAHRAIYAAHTEATGARNGI
GLVKLMGRDSGFIAAYSTLVNSQVNFCLVPEVPFTLAGFLKSLEERLALRGHAVIAVAEGAGQELLPAATESDASGNVKL
GDIGTFLRDAINGHFRAIGTEITLKYIDPSYIIRSQPANSHDSAFCLLLGHNAVHAGMCGRTCMVVGNWNQQFTHVPIRL
ATHQRKKIDPKGRLWSSVLASTGQSREID
>Mature_429_residues
MNHQEPDFNVERLGECRFPSPMRGVRFTTDGERILYHSRYQEMRPFIDSGREPPSMEMGGPRERIFFDPSTIACGIVTCG
GLCPGTNDVIRALTLSLYHHYGVRRVYGFRYGFEGVVKRHGHSPLLLTPEVVDRIGELGGTILASSRGPQAPSEMVDYLE
ELGIAILFTIGGDGTLKGAAKIAEEAARRGRPISVIGIPKTIDNDVSFLQNTFGFETAVDEAHRAIYAAHTEATGARNGI
GLVKLMGRDSGFIAAYSTLVNSQVNFCLVPEVPFTLAGFLKSLEERLALRGHAVIAVAEGAGQELLPAATESDASGNVKL
GDIGTFLRDAINGHFRAIGTEITLKYIDPSYIIRSQPANSHDSAFCLLLGHNAVHAGMCGRTCMVVGNWNQQFTHVPIRL
ATHQRKKIDPKGRLWSSVLASTGQSREID

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI48762920, Length=257, Percent_Identity=30.3501945525292, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI11321601, Length=255, Percent_Identity=28.6274509803922, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI266453619, Length=298, Percent_Identity=26.8456375838926, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI266453768, Length=298, Percent_Identity=26.8456375838926, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI266453748, Length=298, Percent_Identity=26.8456375838926, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI4505749, Length=298, Percent_Identity=26.8456375838926, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1790350, Length=232, Percent_Identity=30.6034482758621, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI25147584, Length=282, Percent_Identity=28.0141843971631, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323861, Length=303, Percent_Identity=25.7425742574257, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6321679, Length=330, Percent_Identity=24.8484848484848, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI28573326, Length=253, Percent_Identity=27.6679841897233, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.90 [H]

Molecular weight: Translated: 46750; Mature: 46750

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHQEPDFNVERLGECRFPSPMRGVRFTTDGERILYHSRYQEMRPFIDSGREPPSMEMGG
CCCCCCCCCHHHHCCCCCCCCCCCCEEECCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCC
PRERIFFDPSTIACGIVTCGGLCPGTNDVIRALTLSLYHHYGVRRVYGFRYGFEGVVKRH
CCCEEEECCCHHEEHHHCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHCCHHHHHHHC
GHSPLLLTPEVVDRIGELGGTILASSRGPQAPSEMVDYLEELGIAILFTIGGDGTLKGAA
CCCCEEECHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHCCEEEEEEECCCCCCHHHH
KIAEEAARRGRPISVIGIPKTIDNDVSFLQNTFGFETAVDEAHRAIYAAHTEATGARNGI
HHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHEEEECCCCCCCCCCC
GLVKLMGRDSGFIAAYSTLVNSQVNFCLVPEVPFTLAGFLKSLEERLALRGHAVIAVAEG
EEEEEECCCCCHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEEECC
AGQELLPAATESDASGNVKLGDIGTFLRDAINGHFRAIGTEITLKYIDPSYIIRSQPANS
CCCCCCCCCCCCCCCCCEEECHHHHHHHHHHCCCEEEEEEEEEEEEECCCEEEECCCCCC
HDSAFCLLLGHNAVHAGMCGRTCMVVGNWNQQFTHVPIRLATHQRKKIDPKGRLWSSVLA
CCCEEEEEEECCCCCCCCCCCEEEEEECCCCCEEEEEEEEECCHHHCCCCCHHHHHHHHH
STGQSREID
CCCCCCCCC
>Mature Secondary Structure
MNHQEPDFNVERLGECRFPSPMRGVRFTTDGERILYHSRYQEMRPFIDSGREPPSMEMGG
CCCCCCCCCHHHHCCCCCCCCCCCCEEECCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCC
PRERIFFDPSTIACGIVTCGGLCPGTNDVIRALTLSLYHHYGVRRVYGFRYGFEGVVKRH
CCCEEEECCCHHEEHHHCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHCCHHHHHHHC
GHSPLLLTPEVVDRIGELGGTILASSRGPQAPSEMVDYLEELGIAILFTIGGDGTLKGAA
CCCCEEECHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHCCEEEEEEECCCCCCHHHH
KIAEEAARRGRPISVIGIPKTIDNDVSFLQNTFGFETAVDEAHRAIYAAHTEATGARNGI
HHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHEEEECCCCCCCCCCC
GLVKLMGRDSGFIAAYSTLVNSQVNFCLVPEVPFTLAGFLKSLEERLALRGHAVIAVAEG
EEEEEECCCCCHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEEECC
AGQELLPAATESDASGNVKLGDIGTFLRDAINGHFRAIGTEITLKYIDPSYIIRSQPANS
CCCCCCCCCCCCCCCCCEEECHHHHHHHHHHCCCEEEEEEEEEEEEECCCEEEECCCCCC
HDSAFCLLLGHNAVHAGMCGRTCMVVGNWNQQFTHVPIRLATHQRKKIDPKGRLWSSVLA
CCCEEEEEEECCCCCCCCCCCEEEEEECCCCCEEEEEEEEECCHHHCCCCCHHHHHHHHH
STGQSREID
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10913106 [H]