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The map label for this gene is xthA [H]

Identifier: 118580060

GI number: 118580060

Start: 1753494

End: 1754306

Strand: Direct

Name: xthA [H]

Synonym: Ppro_1637

Alternate gene names: 118580060

Gene position: 1753494-1754306 (Clockwise)

Preceding gene: 118580059

Following gene: 118580061

Centisome position: 43.75

GC content: 61.38

Gene sequence:

>813_bases
ATGAAAATTGTGTCCTTTAACGTCAATGGCCTGCGCTCGCGCCTCCACCAACTGGAGGCGCTGATCAATGCCCAGCGACC
TGAGATCATCGCCCTGCAGGAGACCAAGGTGCGGGACGAGGAGTTTCCCGTGGATGCTGTCCAGTCGCTGGGGTACCACG
TTGTCTTCCATGGCCAGAAGACCCACTACGGAGTGGCGTTGCTTTCGCGCCTCCCTCTTCAGGACGTGCAGTTAGGACTG
CCGGGCGATGGGGACGAGGCCCAGAAGCGCTTCATCGCCGCAACCATACCCTTAAGTGACGGAACGCCGGTGCGGGTGAT
CAACGGCTACTTCCCCCAGGGTGAGAACCGCGACCATGCTGTCAAGTTCCCGGCAAAGCAGCGCTTCTACGCCGAACTGC
TCGGTCACCTGCAAGGGGAGTGCGATCCCGCCACCCCCCTGGTGGTGCTGGGCGATTTCAACGTGGCTCCCCGGGATATG
GACCTTGGTATCGGCACCGATAATGTCAAGCGCTGGCTGAAGAGCGGCAAGTGCTGTTTCCTCCCCGAGGAGCGACTCTG
GTTTGAGTGCCTGATAGAGTGGGGACTGCACGACTCCTTCCGCGAACTCAACCCCGCAGTGGCGGACCGCTTCAGCTGGT
TCGATTACCGTAGCCGCGGTTTCGAGGGGGAGCCCAAACGAGGACTGCGCATCGACCACATCCTGCTCACCCGATCTCTG
CTTGCGCGCTGCCGCGCGGCGGGCATCGATTACGACATCCGCTCCATGGCGAAACCCTCTGACCACTGTCCGGTGTGGGT
GGAGATTGAGTAG

Upstream 100 bases:

>100_bases
GCTATTCGTAGAGAGCCTGTGAAACACACAGCGCCCCTGTCCGAATCAACGTGCGGACAGGGGCGCTGTTTTTGAACGTC
AAGAGGGTGGGTGAAAACGA

Downstream 100 bases:

>100_bases
AGGGGCGGTAGCTGAATGAAACAGGCAGGCGGGAAGTCTGGCTGCCTGAACGCTGCACGGCTGAGCGTGCTGTTCGGTAG
GGCTCGTCATGGGCGGGCTG

Product: exonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MKIVSFNVNGLRSRLHQLEALINAQRPEIIALQETKVRDEEFPVDAVQSLGYHVVFHGQKTHYGVALLSRLPLQDVQLGL
PGDGDEAQKRFIAATIPLSDGTPVRVINGYFPQGENRDHAVKFPAKQRFYAELLGHLQGECDPATPLVVLGDFNVAPRDM
DLGIGTDNVKRWLKSGKCCFLPEERLWFECLIEWGLHDSFRELNPAVADRFSWFDYRSRGFEGEPKRGLRIDHILLTRSL
LARCRAAGIDYDIRSMAKPSDHCPVWVEIE

Sequences:

>Translated_270_residues
MKIVSFNVNGLRSRLHQLEALINAQRPEIIALQETKVRDEEFPVDAVQSLGYHVVFHGQKTHYGVALLSRLPLQDVQLGL
PGDGDEAQKRFIAATIPLSDGTPVRVINGYFPQGENRDHAVKFPAKQRFYAELLGHLQGECDPATPLVVLGDFNVAPRDM
DLGIGTDNVKRWLKSGKCCFLPEERLWFECLIEWGLHDSFRELNPAVADRFSWFDYRSRGFEGEPKRGLRIDHILLTRSL
LARCRAAGIDYDIRSMAKPSDHCPVWVEIE
>Mature_270_residues
MKIVSFNVNGLRSRLHQLEALINAQRPEIIALQETKVRDEEFPVDAVQSLGYHVVFHGQKTHYGVALLSRLPLQDVQLGL
PGDGDEAQKRFIAATIPLSDGTPVRVINGYFPQGENRDHAVKFPAKQRFYAELLGHLQGECDPATPLVVLGDFNVAPRDM
DLGIGTDNVKRWLKSGKCCFLPEERLWFECLIEWGLHDSFRELNPAVADRFSWFDYRSRGFEGEPKRGLRIDHILLTRSL
LARCRAAGIDYDIRSMAKPSDHCPVWVEIE

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=275, Percent_Identity=30.5454545454545, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI18375503, Length=275, Percent_Identity=30.5454545454545, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI18375501, Length=275, Percent_Identity=30.5454545454545, Blast_Score=88, Evalue=9e-18,
Organism=Escherichia coli, GI1788046, Length=267, Percent_Identity=57.3033707865169, Blast_Score=324, Evalue=4e-90,
Organism=Caenorhabditis elegans, GI71989536, Length=275, Percent_Identity=25.4545454545455, Blast_Score=77, Evalue=9e-15,
Organism=Drosophila melanogaster, GI221330655, Length=274, Percent_Identity=27.7372262773723, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17136678, Length=274, Percent_Identity=27.7372262773723, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30632; Mature: 30632

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVSFNVNGLRSRLHQLEALINAQRPEIIALQETKVRDEEFPVDAVQSLGYHVVFHGQK
CEEEEECHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHCCEEEEEECCC
THYGVALLSRLPLQDVQLGLPGDGDEAQKRFIAATIPLSDGTPVRVINGYFPQGENRDHA
CHHHHHHHHHCCHHHEEECCCCCCHHHHHEEEEEEEECCCCCCEEEEECCCCCCCCCCCE
VKFPAKQRFYAELLGHLQGECDPATPLVVLGDFNVAPRDMDLGIGTDNVKRWLKSGKCCF
EECCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCEEE
LPEERLWFECLIEWGLHDSFRELNPAVADRFSWFDYRSRGFEGEPKRGLRIDHILLTRSL
CCHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
LARCRAAGIDYDIRSMAKPSDHCPVWVEIE
HHHHHHCCCCHHHHHHCCCCCCCCEEEEEC
>Mature Secondary Structure
MKIVSFNVNGLRSRLHQLEALINAQRPEIIALQETKVRDEEFPVDAVQSLGYHVVFHGQK
CEEEEECHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHCCEEEEEECCC
THYGVALLSRLPLQDVQLGLPGDGDEAQKRFIAATIPLSDGTPVRVINGYFPQGENRDHA
CHHHHHHHHHCCHHHEEECCCCCCHHHHHEEEEEEEECCCCCCEEEEECCCCCCCCCCCE
VKFPAKQRFYAELLGHLQGECDPATPLVVLGDFNVAPRDMDLGIGTDNVKRWLKSGKCCF
EECCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCEEE
LPEERLWFECLIEWGLHDSFRELNPAVADRFSWFDYRSRGFEGEPKRGLRIDHILLTRSL
CCHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
LARCRAAGIDYDIRSMAKPSDHCPVWVEIE
HHHHHHCCCCHHHHHHCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]