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The map label for this gene is btuB [H]

Identifier: 118579964

GI number: 118579964

Start: 1643912

End: 1645909

Strand: Reverse

Name: btuB [H]

Synonym: Ppro_1541

Alternate gene names: 118579964

Gene position: 1645909-1643912 (Counterclockwise)

Preceding gene: 118579965

Following gene: 118579963

Centisome position: 41.07

GC content: 58.36

Gene sequence:

>1998_bases
ATGAACACGAAAATCACGGTTGGGGCCGTGTGTCTTGCCGCATGCGGCCTGTTGGCACCCCAGCACAGCGTACTGGCGGC
CCAGCAGGGGGATGAGGACACGGAGGCGTACAACCTGGGCGAGATCGTCGTCTCGGGCAAAACCGAGGGGGTCCAGGCGG
CGGAAACGGTGCATACGGTCACCGCCGAGGACATCAGGAGCATGAATGCCACAACCCTGGATCAGGCAATCTCCCTGCTG
CCCGGCGTGAATGTGCGCATCGGGCCCGAAGGGGTGCCCCGCATCGACGTGAGAGGTTTCCGCACCCGTCATGTCATCCT
GCTGCTGAACGGGGTTCCCCTCAATTCGGCCCTTGACGGCCAGTTCGACCCGACCACCATCCCGACGGAAAACATCGCCA
TGATCAAAATGACCAGCGGAGCCAGTTCCGTCCTCTATGGCCAGGGGGGGCTGGGGGGGGTGATCAACATCATCACCAAG
AAGGGCTCCAAAGATATCAGCGGCATGGTTTCCGCCGAAACCGGCGACCACTACCCCTACCTGGTGAGGTCCAGCCTGGC
CGCCAAGAAGGACGCCTTCGACTTCTTCATCAGCGGAAGCTCCACCGACGTGGACGGTTTCCCGCTCTCCGACAGCTTCG
ATAAAACCTCGGTCCAGGACAGCGACTACCGCAAGAACAGCGACAGGGAACGCCACAACGTGCTGGGCACCATCGGCTAC
AATCCCAACGATGACCTCTCCCTGGGGTTCACCTTCACCTACAACCAGGGGGAGTACGGCAAGCCGCCAGGCACGATCGA
CAACAACAAAGACCCGTTCGCCTCAACACCCAAGTATGAGCGGATAGAGTCCTACGAGGGGCTGTCGCTGCAGCTGGCCG
GTGACTACCGGTTTACCGACAAACTGAACCTGAGGGGCTGGGCCTACCGCAACCAGCTGCACATGCAGGACTACCTGTAC
GCCGACGCCGACTATGACTCCTTCACCAAAAAGAGCAGCTTCAAGGAGCGGGTGAGGACAACCATCCACGGCATCAGCCT
GCAGCCCAGGTACGATTTCGGCGCGGCCGGCACCCTGACCTTCTCCACGATCAACGAATGGGACCAGTGGAAGAACGACG
GCTTGACATCATCGGCCAACAATAAGCCGCTTGATACGAGCAGGGACGAGTCAAAGCAGTACAGCGTCCATACCCTGGCA
GCGGAGTACGAGTTAAGCCCCTTGGAGGGGCTGGGGCTGGTTGCCGGGTACGGCCACCACTGGCAGGCACGCGAACACTC
CTCGGACAACGACTACAGCGTCAACGCCGGCATCCATTACGATATCCTCAAGGACACCCGGCTGAAGTTCTCCTTCAACA
GGAACATCCGTTTCCCCACCCTGGGCGACCTGTACGGTCTCTCCGAAGGCAACCCTGACCTGGATACGGAACGTTCCCTG
ACATTTGAGGGGGGGGTGGAGCAGAAGCTCCCCTTCAACAGCAGGATCAGCCTGATCGGCTTCCATACCGAGGTAAAGGA
CCTGATTCAAAAAGACAAGACCACCGACACGAACATGAACGTGTCCAAGGTTGTGTACGATGGTTTCGAAATCGCGGCAG
CCACCCAGTTCACCAAGAACATCCTCCTGCGCGCCTCCTACACCTATCTGGACTCAAAGGTCAAGGATGACAGCGAAAAG
GAGCAGCTGCAGTACACCCCCCACGACAAGTTCACCTTCGAGGGAAAATACGATTTCGACTGCGGGTTTACCCCCTATGT
CTCGGTCCAGTACGTGGCCAACCAGTATTTCTACACAAAGGATACCGCCGCAACGTTTGACAAGGGGAAGCTGAACGACT
ATGTCCTGCTGAACGTCAAACTGAGCCAAAGGTTCTTCGACAACAGATTCACGACCTATTTCGGGGTCAACAACATCTTC
GACGAGAACTACGAGACAAGCTACGGCTATCCCCAGGCGGGCCGTTTCATCTACGGCGGGATCGAATTGCGCATCTGA

Upstream 100 bases:

>100_bases
ATTACTAATACTATATAACTACGTCTGTTTTTCATGCCGCTATGCGGCACCTGTTATTTTTTTGCGATCTGAACACACCA
CACCTGGAGGGAAACACACT

Downstream 100 bases:

>100_bases
CCGTCGCCACTTCAGCACGGGAACAGTTGGCAATCCCGTGCCATACATGGACGCACCATGCTCCAGGTTAACGATCTATC
CTACAACTATCCGGATGAAC

Product: TonB-dependent receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 665; Mature: 665

Protein sequence:

>665_residues
MNTKITVGAVCLAACGLLAPQHSVLAAQQGDEDTEAYNLGEIVVSGKTEGVQAAETVHTVTAEDIRSMNATTLDQAISLL
PGVNVRIGPEGVPRIDVRGFRTRHVILLLNGVPLNSALDGQFDPTTIPTENIAMIKMTSGASSVLYGQGGLGGVINIITK
KGSKDISGMVSAETGDHYPYLVRSSLAAKKDAFDFFISGSSTDVDGFPLSDSFDKTSVQDSDYRKNSDRERHNVLGTIGY
NPNDDLSLGFTFTYNQGEYGKPPGTIDNNKDPFASTPKYERIESYEGLSLQLAGDYRFTDKLNLRGWAYRNQLHMQDYLY
ADADYDSFTKKSSFKERVRTTIHGISLQPRYDFGAAGTLTFSTINEWDQWKNDGLTSSANNKPLDTSRDESKQYSVHTLA
AEYELSPLEGLGLVAGYGHHWQAREHSSDNDYSVNAGIHYDILKDTRLKFSFNRNIRFPTLGDLYGLSEGNPDLDTERSL
TFEGGVEQKLPFNSRISLIGFHTEVKDLIQKDKTTDTNMNVSKVVYDGFEIAAATQFTKNILLRASYTYLDSKVKDDSEK
EQLQYTPHDKFTFEGKYDFDCGFTPYVSVQYVANQYFYTKDTAATFDKGKLNDYVLLNVKLSQRFFDNRFTTYFGVNNIF
DENYETSYGYPQAGRFIYGGIELRI

Sequences:

>Translated_665_residues
MNTKITVGAVCLAACGLLAPQHSVLAAQQGDEDTEAYNLGEIVVSGKTEGVQAAETVHTVTAEDIRSMNATTLDQAISLL
PGVNVRIGPEGVPRIDVRGFRTRHVILLLNGVPLNSALDGQFDPTTIPTENIAMIKMTSGASSVLYGQGGLGGVINIITK
KGSKDISGMVSAETGDHYPYLVRSSLAAKKDAFDFFISGSSTDVDGFPLSDSFDKTSVQDSDYRKNSDRERHNVLGTIGY
NPNDDLSLGFTFTYNQGEYGKPPGTIDNNKDPFASTPKYERIESYEGLSLQLAGDYRFTDKLNLRGWAYRNQLHMQDYLY
ADADYDSFTKKSSFKERVRTTIHGISLQPRYDFGAAGTLTFSTINEWDQWKNDGLTSSANNKPLDTSRDESKQYSVHTLA
AEYELSPLEGLGLVAGYGHHWQAREHSSDNDYSVNAGIHYDILKDTRLKFSFNRNIRFPTLGDLYGLSEGNPDLDTERSL
TFEGGVEQKLPFNSRISLIGFHTEVKDLIQKDKTTDTNMNVSKVVYDGFEIAAATQFTKNILLRASYTYLDSKVKDDSEK
EQLQYTPHDKFTFEGKYDFDCGFTPYVSVQYVANQYFYTKDTAATFDKGKLNDYVLLNVKLSQRFFDNRFTTYFGVNNIF
DENYETSYGYPQAGRFIYGGIELRI
>Mature_665_residues
MNTKITVGAVCLAACGLLAPQHSVLAAQQGDEDTEAYNLGEIVVSGKTEGVQAAETVHTVTAEDIRSMNATTLDQAISLL
PGVNVRIGPEGVPRIDVRGFRTRHVILLLNGVPLNSALDGQFDPTTIPTENIAMIKMTSGASSVLYGQGGLGGVINIITK
KGSKDISGMVSAETGDHYPYLVRSSLAAKKDAFDFFISGSSTDVDGFPLSDSFDKTSVQDSDYRKNSDRERHNVLGTIGY
NPNDDLSLGFTFTYNQGEYGKPPGTIDNNKDPFASTPKYERIESYEGLSLQLAGDYRFTDKLNLRGWAYRNQLHMQDYLY
ADADYDSFTKKSSFKERVRTTIHGISLQPRYDFGAAGTLTFSTINEWDQWKNDGLTSSANNKPLDTSRDESKQYSVHTLA
AEYELSPLEGLGLVAGYGHHWQAREHSSDNDYSVNAGIHYDILKDTRLKFSFNRNIRFPTLGDLYGLSEGNPDLDTERSL
TFEGGVEQKLPFNSRISLIGFHTEVKDLIQKDKTTDTNMNVSKVVYDGFEIAAATQFTKNILLRASYTYLDSKVKDDSEK
EQLQYTPHDKFTFEGKYDFDCGFTPYVSVQYVANQYFYTKDTAATFDKGKLNDYVLLNVKLSQRFFDNRFTTYFGVNNIF
DENYETSYGYPQAGRFIYGGIELRI

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG1629

COG function: function code P; Outer membrane receptor proteins, mostly Fe transport

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788478, Length=629, Percent_Identity=23.5294117647059, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1786798, Length=229, Percent_Identity=29.2576419213974, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1790405, Length=224, Percent_Identity=31.25, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 74198; Mature: 74198

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTKITVGAVCLAACGLLAPQHSVLAAQQGDEDTEAYNLGEIVVSGKTEGVQAAETVHTV
CCCEEEHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEECEEEEECCCCCCCHHHHHEEE
TAEDIRSMNATTLDQAISLLPGVNVRIGPEGVPRIDVRGFRTRHVILLLNGVPLNSALDG
EHHHHHCCCCHHHHHHHHHCCCCEEEECCCCCCEEECCCCCEEEEEEEEECCCCCCCCCC
QFDPTTIPTENIAMIKMTSGASSVLYGQGGLGGVINIITKKGSKDISGMVSAETGDHYPY
CCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCEEEEEECCCCCCCCCEEECCCCCCCCH
LVRSSLAAKKDAFDFFISGSSTDVDGFPLSDSFDKTSVQDSDYRKNSDRERHNVLGTIGY
HHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCEEEEECC
NPNDDLSLGFTFTYNQGEYGKPPGTIDNNKDPFASTPKYERIESYEGLSLQLAGDYRFTD
CCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEEECCEEEEE
KLNLRGWAYRNQLHMQDYLYADADYDSFTKKSSFKERVRTTIHGISLQPRYDFGAAGTLT
EECCCEEEECCEECHHHEEEECCCCHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCEEE
FSTINEWDQWKNDGLTSSANNKPLDTSRDESKQYSVHTLAAEYELSPLEGLGLVAGYGHH
EEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCEEEECCCCC
WQAREHSSDNDYSVNAGIHYDILKDTRLKFSFNRNIRFPTLGDLYGLSEGNPDLDTERSL
CCCCCCCCCCCEEEECCEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEE
TFEGGVEQKLPFNSRISLIGFHTEVKDLIQKDKTTDTNMNVSKVVYDGFEIAAATQFTKN
EECCCCCCCCCCCCCEEEEEEHHHHHHHHHHCCCCCCCCCCHHEEECCCCEEHHHHHHHH
ILLRASYTYLDSKVKDDSEKEQLQYTPHDKFTFEGKYDFDCGFTPYVSVQYVANQYFYTK
EEEEEEEHHHCCCCCCCCCHHHEEECCCCCEEECCCCCCCCCCCCCEEEEEEECEEEEEC
DTAATFDKGKLNDYVLLNVKLSQRFFDNRFTTYFGVNNIFDENYETSYGYPQAGRFIYGG
CCCCCCCCCCCCCEEEEEEEEHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCEEEEE
IELRI
EEEEC
>Mature Secondary Structure
MNTKITVGAVCLAACGLLAPQHSVLAAQQGDEDTEAYNLGEIVVSGKTEGVQAAETVHTV
CCCEEEHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEECEEEEECCCCCCCHHHHHEEE
TAEDIRSMNATTLDQAISLLPGVNVRIGPEGVPRIDVRGFRTRHVILLLNGVPLNSALDG
EHHHHHCCCCHHHHHHHHHCCCCEEEECCCCCCEEECCCCCEEEEEEEEECCCCCCCCCC
QFDPTTIPTENIAMIKMTSGASSVLYGQGGLGGVINIITKKGSKDISGMVSAETGDHYPY
CCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCEEEEEECCCCCCCCCEEECCCCCCCCH
LVRSSLAAKKDAFDFFISGSSTDVDGFPLSDSFDKTSVQDSDYRKNSDRERHNVLGTIGY
HHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCEEEEECC
NPNDDLSLGFTFTYNQGEYGKPPGTIDNNKDPFASTPKYERIESYEGLSLQLAGDYRFTD
CCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEEECCEEEEE
KLNLRGWAYRNQLHMQDYLYADADYDSFTKKSSFKERVRTTIHGISLQPRYDFGAAGTLT
EECCCEEEECCEECHHHEEEECCCCHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCEEE
FSTINEWDQWKNDGLTSSANNKPLDTSRDESKQYSVHTLAAEYELSPLEGLGLVAGYGHH
EEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCEEEECCCCC
WQAREHSSDNDYSVNAGIHYDILKDTRLKFSFNRNIRFPTLGDLYGLSEGNPDLDTERSL
CCCCCCCCCCCEEEECCEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEE
TFEGGVEQKLPFNSRISLIGFHTEVKDLIQKDKTTDTNMNVSKVVYDGFEIAAATQFTKN
EECCCCCCCCCCCCCEEEEEEHHHHHHHHHHCCCCCCCCCCHHEEECCCCEEHHHHHHHH
ILLRASYTYLDSKVKDDSEKEQLQYTPHDKFTFEGKYDFDCGFTPYVSVQYVANQYFYTK
EEEEEEEHHHCCCCCCCCCHHHEEECCCCCEEECCCCCCCCCCCCCEEEEEEECEEEEEC
DTAATFDKGKLNDYVLLNVKLSQRFFDNRFTTYFGVNNIFDENYETSYGYPQAGRFIYGG
CCCCCCCCCCCCCEEEEEEEEHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCEEEEE
IELRI
EEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA