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The map label for this gene is bclA [H]

Identifier: 118579947

GI number: 118579947

Start: 1627899

End: 1629410

Strand: Reverse

Name: bclA [H]

Synonym: Ppro_1524

Alternate gene names: 118579947

Gene position: 1629410-1627899 (Counterclockwise)

Preceding gene: 118579948

Following gene: 118579946

Centisome position: 40.65

GC content: 66.93

Gene sequence:

>1512_bases
ATGTACACCGACAATGCCGCTGCCATGCTGCTGGAGCGCAACCGGGAACGCCACCCGGACAAGGCCGCCTTTCTCTGCGG
TGAGCGTTCCCTCTCCTACCGGGAGTTGGAGCGGTACAGCCGCCTTTTCGCCCTGCTGCTGCGGGAGCGGGGTATTTCAC
CGGGAGAACGGGTGCTGATCCTGCTCCCCGACAGCCTGGCTTTCCCGGTGGCCTTCCTCGGCTGCCTGCTGGCCGGGGTG
ACGGCGGTGGTCGCAGGCACCGCCCTTACCCGGGAACAGTTCGAGCACATCTTGAACGACAGTGGCAGTCGCCTGCTGGT
GACCGATCCCCGGCTCCCCTCCCCGCTCCCGCCGGAACAGAGCCGGGTCGGCCTGCTCGTCTGCGACGATAGGGACAGAT
TCGAACCACCCGAGGGGCACGACGGGGAGTTCGCGGCGGACGTTCCTCCGGCCGACCGGATCGCCTACATGCAGTACAGC
TCCGGCTCCACCGGAGTACCCAAGGGGATCCCCCACCGCCACCGGGATCTGCTGATCCCCTGCGACCTGGTGGGCAGGGA
GATCCTGGGGCTCAGCGAACAGGACCGCATCTTCTCGGCAAGCAAGTTCTCCTTTGCCTACGGCCTGATCAACAGCCTGG
CCTTCCCGCTCTTCTTCGGGGCAACGGCGATCCTCATTCCGGACAAGCCGGACGCGGCCGCCATTCTGGAGCTAATGGAG
CGCCACCGGCCGAGCGTCTTCTTCTCCGTGCCCACGGTCTACTCCCAGCTGATCCTCTCCTGCGGCTCCGACCGCCTGGA
ACTCTCCCTGCGACTCTGCTACTCCGCCGGCGAGGCCCTGCCGGCAGCCATCTTCCACGAGTGGCACCGCCTGACCGGGC
TTGAGCTGCTGGACGGCATCGGCTCCACCGAAATGTCCTACATCTACATTTCCAACCGCCCCGGCCGGGCCAGGCCCGGT
TCCGCCGGCCAGGCGGTGCCCGGCTACCGGATCAGGCTGGTGGACGAGGACGGCAGGGCGGTGCCCTCCGGAACAGTGGG
CACCCTGCTGGTCAAGGGGCCGACCAGCTGCCCCTGCTACTGGAACCTGCCGGACAAAAGCGGCGAGACCATGCTCCCCG
ACGGTTTCCTGCGCAGTGGCGACCTGTTCCTGGAACGGGACGGTTTCTACTACCACCAGGGACGCAACGACGATATGATC
AAGGCCGGCGCCCAGTTGGTCTCGCCGCTGCCGGTGGAGGAGGCGCTCCTGAGTCACCCCAGCGTGGCCGAATGCGCCGT
GGTAGCCCTATCCGTGGGCGGCCTGGTCAGGCCGGGGGCCTTCGTGATACCATCCCCCGAGGTCCAGGGGAACCCGGCAC
TGGCGCGCCGCCTGCGCGCCCATCTGCTGGAGCGGCTGCCGGAGTACATGTGTCCGGTCAGCTTCACCTTCGTGGAACAG
CTTCCCCGTACACCCACCGGCAAGATCCAGCGTTTTCGGCTGCGAGAACCGGCTCAACCGAAAGGAGCATAG

Upstream 100 bases:

>100_bases
TGATGTGCCCCTCCCCCTGACAGCGGCGCGCATGGGAAAGACTCGGGAAAACCCGGCCCTGTCCCTGCCGGTAATGAGAT
GATATTCACGGAGGAAAACA

Downstream 100 bases:

>100_bases
ACATGGAACTGATCGAGGAACTGAAACAGCTGATGCTGGAGGTGGGTGTGGAGCCGGCCGTCGTGGAACAGCTGGACCCT
TCCCGGCTACTGGCCCAGCA

Product: benzoate-CoA ligase family

Products: NA

Alternate protein names: Benzoyl-CoA synthetase [H]

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MYTDNAAAMLLERNRERHPDKAAFLCGERSLSYRELERYSRLFALLLRERGISPGERVLILLPDSLAFPVAFLGCLLAGV
TAVVAGTALTREQFEHILNDSGSRLLVTDPRLPSPLPPEQSRVGLLVCDDRDRFEPPEGHDGEFAADVPPADRIAYMQYS
SGSTGVPKGIPHRHRDLLIPCDLVGREILGLSEQDRIFSASKFSFAYGLINSLAFPLFFGATAILIPDKPDAAAILELME
RHRPSVFFSVPTVYSQLILSCGSDRLELSLRLCYSAGEALPAAIFHEWHRLTGLELLDGIGSTEMSYIYISNRPGRARPG
SAGQAVPGYRIRLVDEDGRAVPSGTVGTLLVKGPTSCPCYWNLPDKSGETMLPDGFLRSGDLFLERDGFYYHQGRNDDMI
KAGAQLVSPLPVEEALLSHPSVAECAVVALSVGGLVRPGAFVIPSPEVQGNPALARRLRAHLLERLPEYMCPVSFTFVEQ
LPRTPTGKIQRFRLREPAQPKGA

Sequences:

>Translated_503_residues
MYTDNAAAMLLERNRERHPDKAAFLCGERSLSYRELERYSRLFALLLRERGISPGERVLILLPDSLAFPVAFLGCLLAGV
TAVVAGTALTREQFEHILNDSGSRLLVTDPRLPSPLPPEQSRVGLLVCDDRDRFEPPEGHDGEFAADVPPADRIAYMQYS
SGSTGVPKGIPHRHRDLLIPCDLVGREILGLSEQDRIFSASKFSFAYGLINSLAFPLFFGATAILIPDKPDAAAILELME
RHRPSVFFSVPTVYSQLILSCGSDRLELSLRLCYSAGEALPAAIFHEWHRLTGLELLDGIGSTEMSYIYISNRPGRARPG
SAGQAVPGYRIRLVDEDGRAVPSGTVGTLLVKGPTSCPCYWNLPDKSGETMLPDGFLRSGDLFLERDGFYYHQGRNDDMI
KAGAQLVSPLPVEEALLSHPSVAECAVVALSVGGLVRPGAFVIPSPEVQGNPALARRLRAHLLERLPEYMCPVSFTFVEQ
LPRTPTGKIQRFRLREPAQPKGA
>Mature_503_residues
MYTDNAAAMLLERNRERHPDKAAFLCGERSLSYRELERYSRLFALLLRERGISPGERVLILLPDSLAFPVAFLGCLLAGV
TAVVAGTALTREQFEHILNDSGSRLLVTDPRLPSPLPPEQSRVGLLVCDDRDRFEPPEGHDGEFAADVPPADRIAYMQYS
SGSTGVPKGIPHRHRDLLIPCDLVGREILGLSEQDRIFSASKFSFAYGLINSLAFPLFFGATAILIPDKPDAAAILELME
RHRPSVFFSVPTVYSQLILSCGSDRLELSLRLCYSAGEALPAAIFHEWHRLTGLELLDGIGSTEMSYIYISNRPGRARPG
SAGQAVPGYRIRLVDEDGRAVPSGTVGTLLVKGPTSCPCYWNLPDKSGETMLPDGFLRSGDLFLERDGFYYHQGRNDDMI
KAGAQLVSPLPVEEALLSHPSVAECAVVALSVGGLVRPGAFVIPSPEVQGNPALARRLRAHLLERLPEYMCPVSFTFVEQ
LPRTPTGKIQRFRLREPAQPKGA

Specific function: Catalyzes the ligation of benzoate and CoA to form benzoyl-CoA at the expense of ATP. The enzyme also ligates 2- aminobenzoate and CoA. The enzyme shows activity toward a number of benzoate derivates [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. Benzoate-CoA ligase subfamily [H]

Homologues:

Organism=Homo sapiens, GI42544132, Length=512, Percent_Identity=26.7578125, Blast_Score=182, Evalue=6e-46,
Organism=Homo sapiens, GI38505220, Length=515, Percent_Identity=27.5728155339806, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI122937307, Length=514, Percent_Identity=27.8210116731518, Blast_Score=169, Evalue=6e-42,
Organism=Homo sapiens, GI187761345, Length=521, Percent_Identity=28.2149712092131, Blast_Score=162, Evalue=7e-40,
Organism=Homo sapiens, GI187761343, Length=521, Percent_Identity=28.2149712092131, Blast_Score=162, Evalue=7e-40,
Organism=Homo sapiens, GI115511026, Length=518, Percent_Identity=26.4478764478764, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI58082049, Length=512, Percent_Identity=26.171875, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI157311624, Length=512, Percent_Identity=25.78125, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI157311622, Length=512, Percent_Identity=25.78125, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI28416953, Length=562, Percent_Identity=24.0213523131673, Blast_Score=119, Evalue=9e-27,
Organism=Homo sapiens, GI156151445, Length=539, Percent_Identity=23.3766233766234, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI42544134, Length=351, Percent_Identity=25.0712250712251, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI13375727, Length=519, Percent_Identity=22.7360308285164, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI195947378, Length=410, Percent_Identity=22.4390243902439, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI8923896, Length=364, Percent_Identity=26.6483516483516, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI253970489, Length=364, Percent_Identity=26.6483516483516, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI45580730, Length=365, Percent_Identity=24.6575342465753, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI27477105, Length=330, Percent_Identity=24.2424242424242, Blast_Score=70, Evalue=6e-12,
Organism=Escherichia coli, GI1788107, Length=536, Percent_Identity=28.3582089552239, Blast_Score=178, Evalue=6e-46,
Organism=Escherichia coli, GI1786810, Length=503, Percent_Identity=27.2365805168986, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI145693145, Length=528, Percent_Identity=25.7575757575758, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1790505, Length=549, Percent_Identity=26.2295081967213, Blast_Score=136, Evalue=4e-33,
Organism=Escherichia coli, GI1786801, Length=515, Percent_Identity=27.378640776699, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1786529, Length=545, Percent_Identity=25.3211009174312, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1789201, Length=500, Percent_Identity=25, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI221142682, Length=505, Percent_Identity=23.3663366336634, Blast_Score=92, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17558820, Length=513, Percent_Identity=26.3157894736842, Blast_Score=148, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI32563687, Length=497, Percent_Identity=24.9496981891348, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17531443, Length=521, Percent_Identity=24.1842610364683, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17557194, Length=539, Percent_Identity=24.30426716141, Blast_Score=121, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17538037, Length=541, Percent_Identity=24.0295748613678, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71994690, Length=538, Percent_Identity=23.0483271375465, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI71994694, Length=538, Percent_Identity=23.0483271375465, Blast_Score=115, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI71994703, Length=538, Percent_Identity=23.0483271375465, Blast_Score=114, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI17560308, Length=552, Percent_Identity=23.5507246376812, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71982997, Length=560, Percent_Identity=24.1071428571429, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71983001, Length=560, Percent_Identity=24.1071428571429, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI71985884, Length=350, Percent_Identity=26, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17559526, Length=525, Percent_Identity=23.8095238095238, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI25141359, Length=504, Percent_Identity=22.6190476190476, Blast_Score=80, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6319699, Length=525, Percent_Identity=23.2380952380952, Blast_Score=128, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6323182, Length=560, Percent_Identity=23.9285714285714, Blast_Score=117, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6319264, Length=563, Percent_Identity=23.8010657193606, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI62472339, Length=546, Percent_Identity=24.7252747252747, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24667955, Length=546, Percent_Identity=24.7252747252747, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24581924, Length=538, Percent_Identity=24.3494423791822, Blast_Score=118, Evalue=9e-27,
Organism=Drosophila melanogaster, GI18859661, Length=521, Percent_Identity=24.9520153550864, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24667959, Length=433, Percent_Identity=24.9422632794457, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24648260, Length=375, Percent_Identity=24.2666666666667, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21356947, Length=486, Percent_Identity=22.4279835390947, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI19922652, Length=356, Percent_Identity=26.4044943820225, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21355181, Length=489, Percent_Identity=23.3128834355828, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI161076582, Length=528, Percent_Identity=21.2121212121212, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24653035, Length=518, Percent_Identity=21.042471042471, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24656500, Length=357, Percent_Identity=25.7703081232493, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI20130357, Length=352, Percent_Identity=23.2954545454545, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648257, Length=368, Percent_Identity=22.554347826087, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21356441, Length=343, Percent_Identity=23.3236151603499, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648253, Length=278, Percent_Identity=23.7410071942446, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648255, Length=278, Percent_Identity=24.1007194244604, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24648676, Length=374, Percent_Identity=23.2620320855615, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR011957 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.25 [H]

Molecular weight: Translated: 55202; Mature: 55202

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00358 RIBOSOMAL_L5 ; PS00455 AMP_BINDING ; PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYTDNAAAMLLERNRERHPDKAAFLCGERSLSYRELERYSRLFALLLRERGISPGERVLI
CCCCHHHHHHHHHHHHCCCCHHEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEE
LLPDSLAFPVAFLGCLLAGVTAVVAGTALTREQFEHILNDSGSRLLVTDPRLPSPLPPEQ
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCC
SRVGLLVCDDRDRFEPPEGHDGEFAADVPPADRIAYMQYSSGSTGVPKGIPHRHRDLLIP
CCEEEEEECCCCCCCCCCCCCCCEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEE
CDLVGREILGLSEQDRIFSASKFSFAYGLINSLAFPLFFGATAILIPDKPDAAAILELME
HHHHHHHHHCCCCCCCEECHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHH
RHRPSVFFSVPTVYSQLILSCGSDRLELSLRLCYSAGEALPAAIFHEWHRLTGLELLDGI
HCCCCEEEEHHHHHHHHHHHCCCCHHEEEEEEHHHCCCHHHHHHHHHHHHHHHHHHHHCC
GSTEMSYIYISNRPGRARPGSAGQAVPGYRIRLVDEDGRAVPSGTVGTLLVKGPTSCPCY
CCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCEE
WNLPDKSGETMLPDGFLRSGDLFLERDGFYYHQGRNDDMIKAGAQLVSPLPVEEALLSHP
ECCCCCCCCEECCCCCCCCCCEEEEECCEEEECCCCCHHHHHHHHHHCCCCHHHHHHCCC
SVAECAVVALSVGGLVRPGAFVIPSPEVQGNPALARRLRAHLLERLPEYMCPVSFTFVEQ
CHHHHHHHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LPRTPTGKIQRFRLREPAQPKGA
CCCCCCCCHHEEECCCCCCCCCC
>Mature Secondary Structure
MYTDNAAAMLLERNRERHPDKAAFLCGERSLSYRELERYSRLFALLLRERGISPGERVLI
CCCCHHHHHHHHHHHHCCCCHHEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEE
LLPDSLAFPVAFLGCLLAGVTAVVAGTALTREQFEHILNDSGSRLLVTDPRLPSPLPPEQ
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCC
SRVGLLVCDDRDRFEPPEGHDGEFAADVPPADRIAYMQYSSGSTGVPKGIPHRHRDLLIP
CCEEEEEECCCCCCCCCCCCCCCEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEE
CDLVGREILGLSEQDRIFSASKFSFAYGLINSLAFPLFFGATAILIPDKPDAAAILELME
HHHHHHHHHCCCCCCCEECHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHH
RHRPSVFFSVPTVYSQLILSCGSDRLELSLRLCYSAGEALPAAIFHEWHRLTGLELLDGI
HCCCCEEEEHHHHHHHHHHHCCCCHHEEEEEEHHHCCCHHHHHHHHHHHHHHHHHHHHCC
GSTEMSYIYISNRPGRARPGSAGQAVPGYRIRLVDEDGRAVPSGTVGTLLVKGPTSCPCY
CCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCEE
WNLPDKSGETMLPDGFLRSGDLFLERDGFYYHQGRNDDMIKAGAQLVSPLPVEEALLSHP
ECCCCCCCCEECCCCCCCCCCEEEEECCEEEECCCCCHHHHHHHHHHCCCCHHHHHHCCC
SVAECAVVALSVGGLVRPGAFVIPSPEVQGNPALARRLRAHLLERLPEYMCPVSFTFVEQ
CHHHHHHHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LPRTPTGKIQRFRLREPAQPKGA
CCCCCCCCHHEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12897012 [H]