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The map label for this gene is cbiF [H]

Identifier: 118579679

GI number: 118579679

Start: 1307285

End: 1308175

Strand: Direct

Name: cbiF [H]

Synonym: Ppro_1250

Alternate gene names: 118579679

Gene position: 1307285-1308175 (Clockwise)

Preceding gene: 118579678

Following gene: 118579680

Centisome position: 32.62

GC content: 54.99

Gene sequence:

>891_bases
ATGCACATATTCAACAGGCACACCAAGAGATTGCTGGTATTCATGTTTTTCGCGGTACTGGCGCTTCCGTTGTTTTCCTC
TTCAGACGCACTGGCCAGGGATGAGGCCGCAACCCGGCTGCACCTGGTGAGCGTTGGCAGCGGCGACCCGGACAACATCA
CGCTCAAGGCGGTTACTGTGATCAGGGATGCGGACATTGTCTTCTGTGACAAGAGGATCAGGGACAAGTTTCCCGTGTTG
CTTCATGGAAAGGAGATTCACGACATAGGATTCGGCATCTTCGGCATCTTCGGCAAGTCAATGGAAGAGGCGAAGAAGAA
CAAGCGCTTCAACTATGAGGAAAAAACAAAGGAACTGAAGCAGATCGATGCCGTTATCCGCGCTGCCGTCAGGAACGGGA
AGAAGGTGGCGGTCCTGGACAGCGGGGATCCCACCATCTATGGGCCGCACATGTGGTACATGGAGGCGTTTGAAGACCTG
AATCCTGAAGTGGTGCCGGGGATCAGCTGTTTTAACGCTGCTAACGCGGCTTTGAAAAAAGGGGTGACCTCGGGAAAGAA
CACGCATTCGGTCATCCTTACCGCCTCCTTCGGCAGGGAGGATGCCGGCGGCACGGACAGCATCGAGAATCTCTCCGCAA
ACCGGGCCTCCATGGTCTTCTTCACCATGTTTCTCAACATGGATGAGGTGGTGAAGAAACTGAGAGTCCACTATCTGCCC
GAGACACCCATCGCGATCGTTCTCAACGCCGGTTACCGGGAAAAGGAGCGGGTCATCCGGGGTACCCTCGGCACCATTCT
CGCCGAGATGAAGGGTGAGAAGCTTCCCTTCGAGCATCTCATCTACGTCGGTGATTTCCTGACCAACCGTTACCAGGCCA
GCGGGAAATGA

Upstream 100 bases:

>100_bases
GTCCCGAGGACGCTTTTTATCGGGGATACTTCGTATCTCGTCAGGGATTCACGATAGTTCTCCCATTCAATTAACCCAAT
GCGGAAAAGGATTACTTCAA

Downstream 100 bases:

>100_bases
CCGGCAACAACCACCAATTGCAGCCGCTAATGAAAGGACTGGATATGAAACTGATCAGACTGATCCCCCTGTTTGTCTTC
TGCATGACATCCGTGGCCCA

Product: uroporphyrin-III C/tetrapyrrole methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 5

Alternate protein names: Cobalt-precorrin-3 methylase [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MHIFNRHTKRLLVFMFFAVLALPLFSSSDALARDEAATRLHLVSVGSGDPDNITLKAVTVIRDADIVFCDKRIRDKFPVL
LHGKEIHDIGFGIFGIFGKSMEEAKKNKRFNYEEKTKELKQIDAVIRAAVRNGKKVAVLDSGDPTIYGPHMWYMEAFEDL
NPEVVPGISCFNAANAALKKGVTSGKNTHSVILTASFGREDAGGTDSIENLSANRASMVFFTMFLNMDEVVKKLRVHYLP
ETPIAIVLNAGYREKERVIRGTLGTILAEMKGEKLPFEHLIYVGDFLTNRYQASGK

Sequences:

>Translated_296_residues
MHIFNRHTKRLLVFMFFAVLALPLFSSSDALARDEAATRLHLVSVGSGDPDNITLKAVTVIRDADIVFCDKRIRDKFPVL
LHGKEIHDIGFGIFGIFGKSMEEAKKNKRFNYEEKTKELKQIDAVIRAAVRNGKKVAVLDSGDPTIYGPHMWYMEAFEDL
NPEVVPGISCFNAANAALKKGVTSGKNTHSVILTASFGREDAGGTDSIENLSANRASMVFFTMFLNMDEVVKKLRVHYLP
ETPIAIVLNAGYREKERVIRGTLGTILAEMKGEKLPFEHLIYVGDFLTNRYQASGK
>Mature_296_residues
MHIFNRHTKRLLVFMFFAVLALPLFSSSDALARDEAATRLHLVSVGSGDPDNITLKAVTVIRDADIVFCDKRIRDKFPVL
LHGKEIHDIGFGIFGIFGKSMEEAKKNKRFNYEEKTKELKQIDAVIRAAVRNGKKVAVLDSGDPTIYGPHMWYMEAFEDL
NPEVVPGISCFNAANAALKKGVTSGKNTHSVILTASFGREDAGGTDSIENLSANRASMVFFTMFLNMDEVVKKLRVHYLP
ETPIAIVLNAGYREKERVIRGTLGTILAEMKGEKLPFEHLIYVGDFLTNRYQASGK

Specific function: Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5 [H]

COG id: COG2875

COG function: function code H; Precorrin-4 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=246, Percent_Identity=29.6747967479675, Blast_Score=75, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006362
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.133

Molecular weight: Translated: 33014; Mature: 33014

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIFNRHTKRLLVFMFFAVLALPLFSSSDALARDEAATRLHLVSVGSGDPDNITLKAVTV
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCEEEEEEECCCCCCCEEEEEEEE
IRDADIVFCDKRIRDKFPVLLHGKEIHDIGFGIFGIFGKSMEEAKKNKRFNYEEKTKELK
EECCCEEEECHHHHCCCCEEEECCCHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHH
QIDAVIRAAVRNGKKVAVLDSGDPTIYGPHMWYMEAFEDLNPEVVPGISCFNAANAALKK
HHHHHHHHHHHCCCEEEEEECCCCEEECCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
GVTSGKNTHSVILTASFGREDAGGTDSIENLSANRASMVFFTMFLNMDEVVKKLRVHYLP
CCCCCCCCEEEEEEEECCCCCCCCCCCHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHCCC
ETPIAIVLNAGYREKERVIRGTLGTILAEMKGEKLPFEHLIYVGDFLTNRYQASGK
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MHIFNRHTKRLLVFMFFAVLALPLFSSSDALARDEAATRLHLVSVGSGDPDNITLKAVTV
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCEEEEEEECCCCCCCEEEEEEEE
IRDADIVFCDKRIRDKFPVLLHGKEIHDIGFGIFGIFGKSMEEAKKNKRFNYEEKTKELK
EECCCEEEECHHHHCCCCEEEECCCHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHH
QIDAVIRAAVRNGKKVAVLDSGDPTIYGPHMWYMEAFEDLNPEVVPGISCFNAANAALKK
HHHHHHHHHHHCCCEEEEEECCCCEEECCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
GVTSGKNTHSVILTASFGREDAGGTDSIENLSANRASMVFFTMFLNMDEVVKKLRVHYLP
CCCCCCCCEEEEEEEECCCCCCCCCCCHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHCCC
ETPIAIVLNAGYREKERVIRGTLGTILAEMKGEKLPFEHLIYVGDFLTNRYQASGK
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 4

Specific reaction: S-adenosyl-L-methionine + precorrin-4 = S-adenosyl-L-homocysteine + precorrin-5

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]