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The map label for this gene is nrfA [H]

Identifier: 118579629

GI number: 118579629

Start: 1251440

End: 1252903

Strand: Direct

Name: nrfA [H]

Synonym: Ppro_1200

Alternate gene names: 118579629

Gene position: 1251440-1252903 (Clockwise)

Preceding gene: 118579628

Following gene: 118579630

Centisome position: 31.22

GC content: 57.86

Gene sequence:

>1464_bases
ATGAGAATAAGGACCGTTGTGTGTGTCGTTGTCGTTGCGTGTGTGTGGCTGCTCGCCGCCGGCTGCGCTCCCCAGAAGGC
CGAACAGGTAAGGGCGGTGCAGATTGCGGACGGCACCATCGATCCGGCCGAGTGGGGAAAAGCGTATCCGGTGGAATACG
AGATGTGGAAAAAGACGGCCGAACCGCCCGATACTCCCGGGCTGAGCAAGTACAAGAAGGGGAATGACGATCCCAAATCA
ATGGTGGACAAACTCTCCGAGTATCCCTACCTGGCCCTGCTCTTTAACGGCTGGGGTTTCGGGATTGGCTACAACGAACC
GCGCGGGCATTCACACATGGTTCAGGATCAGCTGGATATCGACTCCTCTCGCCTCAAGGCCGGCGGTGTTTGCCTCACCT
GCAAAACCCCCTACGCCAACTATCTCATGAAAACCATGGGGGCCGACTACTACAAGACCCCCTACAAGGAGGTCCTGGCC
AGGATTCCCAATCAGAAGTTCGCCACCCTCAGCGTTGCCTGCATCGATTGCCACAACAACAGGGACATGTCCCTGACTCT
GTCCCGTGACTTCACCCTCGGCGCCGCGCTGCGCGGCATGGGGGTGGATACGGCAAAGCTCGACCACCAGACGAAACGGA
CCCTGGTCTGCGCCCAGTGCCATGTCACCTATGTGATCCCCAAGGACAAGGATATGAAGTCGGTCGGCCTGTTCTTCCCC
TGGCAGGGAAGCAAGCCGGGCAACATCACCATTGAGAACATCATCGCCACGATCAAGAGCGATCCTTCCTATCTGGAGTG
GAAGCAGACCGTAACCGGCTTCAAGTTGGGCTTTATCCGCCACCCCGAATACGAGCTCTACTCCAACAACAGCGTGCACT
GGAAGGGAGGAGCTTCCTGCGCCGACTGCCACATGCCCTTCACCAAGGTCGGTGTTCATAAGGCTTCCGATCACCGCATA
ACCAGCCCGCTGAAAAACGGACTCAAGGGCTGTATCCAGTGCCACGCCGAAAGCCAGGAATGGCTCAAGGAGCAGATCAT
AGCCATTCAGGACAGAACCGTGTCCCTGCAGCTTCGCGCCGGCTATGCGACCGCCACCGTGGCAAAGCTTTTCGAGAAGG
TTCACACCGCCCAGGCGGCAGGCAAGAAAATTGACGCATCGCTCTATGCCATGGCCAAGGAGTATTACGAGCAGGCCTTC
TACCGCTGCATCTTCATCGGCGCCGAAAACTCGATCGGCTTCCACAATCCGACCGAATCGATGCGTGTTCTGGGAGACTC
CATCGCCTTTGCCACCAAGGCCGAAGCGTTTCTCCGTCAGGCGCTGGCCCAGGCCGGGGTAATCGTTCCGGCTAAGGTTG
ACCTTGAACTGGCCAAGTACCTGAACAACAGGGGGGCGGCCAAGTTGAATTTCCGCAAGGAACTGGAGCTTCCCGATCCA
AGCGGCATCCAGAATCGATTCTGA

Upstream 100 bases:

>100_bases
CAACACCGAACGGCACTGCTGGAGCTGCCATCGAGGCATCACCCATCTGCGTAGCGGAGCACGGGTAACACGCTGAATTC
AGTTAAAAGGGGGTATTCCA

Downstream 100 bases:

>100_bases
TTGAGTAATCGCGTATTTTTGACAACAGTCAAACCGTGCCAGGAGAGGGAGGTATATCTTTCACGCATACAAGACGAGAA
ATTCACCAAACCAGTTTCAT

Product: nitrite reductase

Products: NA

Alternate protein names: Ammonia-forming cytochrome c nitrite reductase; Cytochrome c nitrite reductase [H]

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MRIRTVVCVVVVACVWLLAAGCAPQKAEQVRAVQIADGTIDPAEWGKAYPVEYEMWKKTAEPPDTPGLSKYKKGNDDPKS
MVDKLSEYPYLALLFNGWGFGIGYNEPRGHSHMVQDQLDIDSSRLKAGGVCLTCKTPYANYLMKTMGADYYKTPYKEVLA
RIPNQKFATLSVACIDCHNNRDMSLTLSRDFTLGAALRGMGVDTAKLDHQTKRTLVCAQCHVTYVIPKDKDMKSVGLFFP
WQGSKPGNITIENIIATIKSDPSYLEWKQTVTGFKLGFIRHPEYELYSNNSVHWKGGASCADCHMPFTKVGVHKASDHRI
TSPLKNGLKGCIQCHAESQEWLKEQIIAIQDRTVSLQLRAGYATATVAKLFEKVHTAQAAGKKIDASLYAMAKEYYEQAF
YRCIFIGAENSIGFHNPTESMRVLGDSIAFATKAEAFLRQALAQAGVIVPAKVDLELAKYLNNRGAAKLNFRKELELPDP
SGIQNRF

Sequences:

>Translated_487_residues
MRIRTVVCVVVVACVWLLAAGCAPQKAEQVRAVQIADGTIDPAEWGKAYPVEYEMWKKTAEPPDTPGLSKYKKGNDDPKS
MVDKLSEYPYLALLFNGWGFGIGYNEPRGHSHMVQDQLDIDSSRLKAGGVCLTCKTPYANYLMKTMGADYYKTPYKEVLA
RIPNQKFATLSVACIDCHNNRDMSLTLSRDFTLGAALRGMGVDTAKLDHQTKRTLVCAQCHVTYVIPKDKDMKSVGLFFP
WQGSKPGNITIENIIATIKSDPSYLEWKQTVTGFKLGFIRHPEYELYSNNSVHWKGGASCADCHMPFTKVGVHKASDHRI
TSPLKNGLKGCIQCHAESQEWLKEQIIAIQDRTVSLQLRAGYATATVAKLFEKVHTAQAAGKKIDASLYAMAKEYYEQAF
YRCIFIGAENSIGFHNPTESMRVLGDSIAFATKAEAFLRQALAQAGVIVPAKVDLELAKYLNNRGAAKLNFRKELELPDP
SGIQNRF
>Mature_487_residues
MRIRTVVCVVVVACVWLLAAGCAPQKAEQVRAVQIADGTIDPAEWGKAYPVEYEMWKKTAEPPDTPGLSKYKKGNDDPKS
MVDKLSEYPYLALLFNGWGFGIGYNEPRGHSHMVQDQLDIDSSRLKAGGVCLTCKTPYANYLMKTMGADYYKTPYKEVLA
RIPNQKFATLSVACIDCHNNRDMSLTLSRDFTLGAALRGMGVDTAKLDHQTKRTLVCAQCHVTYVIPKDKDMKSVGLFFP
WQGSKPGNITIENIIATIKSDPSYLEWKQTVTGFKLGFIRHPEYELYSNNSVHWKGGASCADCHMPFTKVGVHKASDHRI
TSPLKNGLKGCIQCHAESQEWLKEQIIAIQDRTVSLQLRAGYATATVAKLFEKVHTAQAAGKKIDASLYAMAKEYYEQAF
YRCIFIGAENSIGFHNPTESMRVLGDSIAFATKAEAFLRQALAQAGVIVPAKVDLELAKYLNNRGAAKLNFRKELELPDP
SGIQNRF

Specific function: Plays a role in nitrite reduction [H]

COG id: COG3303

COG function: function code P; Formate-dependent nitrite reductase, periplasmic cytochrome c552 subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome c-552 family [H]

Homologues:

Organism=Escherichia coli, GI1790506, Length=415, Percent_Identity=26.2650602409639, Blast_Score=130, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003321
- InterPro:   IPR017570
- InterPro:   IPR011031 [H]

Pfam domain/function: PF02335 Cytochrom_C552 [H]

EC number: =1.7.2.2 [H]

Molecular weight: Translated: 54144; Mature: 54144

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIRTVVCVVVVACVWLLAAGCAPQKAEQVRAVQIADGTIDPAEWGKAYPVEYEMWKKTA
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHEEEEEEECCCCCHHHCCCCCCCCHHHHHCCC
EPPDTPGLSKYKKGNDDPKSMVDKLSEYPYLALLFNGWGFGIGYNEPRGHSHMVQDQLDI
CCCCCCCCHHHCCCCCCHHHHHHHHHHCCEEEEEEECEEECCCCCCCCCCCHHHHHHHCC
DSSRLKAGGVCLTCKTPYANYLMKTMGADYYKTPYKEVLARIPNQKFATLSVACIDCHNN
CHHHCCCCCEEEEECCCHHHHHHHHHCCCHHCCCHHHHHHHCCCCCEEEEEEEEEEECCC
RDMSLTLSRDFTLGAALRGMGVDTAKLDHQTKRTLVCAQCHVTYVIPKDKDMKSVGLFFP
CCEEEEEECCCCHHHHHHCCCCCHHHHCCHHHHEEEEEEEEEEEEECCCCCCCCCCEEEC
WQGSKPGNITIENIIATIKSDPSYLEWKQTVTGFKLGFIRHPEYELYSNNSVHWKGGASC
CCCCCCCCEEHHHHHHHHCCCCCHHHHHHHHCCEEEEEEECCCEEEECCCEEEECCCCCE
ADCHMPFTKVGVHKASDHRITSPLKNGLKGCIQCHAESQEWLKEQIIAIQDRTVSLQLRA
ECCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHEEEECCEEEEEEEC
GYATATVAKLFEKVHTAQAAGKKIDASLYAMAKEYYEQAFYRCIFIGAENSIGFHNPTES
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCHHH
MRVLGDSIAFATKAEAFLRQALAQAGVIVPAKVDLELAKYLNNRGAAKLNFRKELELPDP
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHCCCCCEEEECCEECCCCCC
SGIQNRF
CCCCCCC
>Mature Secondary Structure
MRIRTVVCVVVVACVWLLAAGCAPQKAEQVRAVQIADGTIDPAEWGKAYPVEYEMWKKTA
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHEEEEEEECCCCCHHHCCCCCCCCHHHHHCCC
EPPDTPGLSKYKKGNDDPKSMVDKLSEYPYLALLFNGWGFGIGYNEPRGHSHMVQDQLDI
CCCCCCCCHHHCCCCCCHHHHHHHHHHCCEEEEEEECEEECCCCCCCCCCCHHHHHHHCC
DSSRLKAGGVCLTCKTPYANYLMKTMGADYYKTPYKEVLARIPNQKFATLSVACIDCHNN
CHHHCCCCCEEEEECCCHHHHHHHHHCCCHHCCCHHHHHHHCCCCCEEEEEEEEEEECCC
RDMSLTLSRDFTLGAALRGMGVDTAKLDHQTKRTLVCAQCHVTYVIPKDKDMKSVGLFFP
CCEEEEEECCCCHHHHHHCCCCCHHHHCCHHHHEEEEEEEEEEEEECCCCCCCCCCEEEC
WQGSKPGNITIENIIATIKSDPSYLEWKQTVTGFKLGFIRHPEYELYSNNSVHWKGGASC
CCCCCCCCEEHHHHHHHHCCCCCHHHHHHHHCCEEEEEEECCCEEEECCCEEEECCCCCE
ADCHMPFTKVGVHKASDHRITSPLKNGLKGCIQCHAESQEWLKEQIIAIQDRTVSLQLRA
ECCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHEEEECCEEEEEEEC
GYATATVAKLFEKVHTAQAAGKKIDASLYAMAKEYYEQAFYRCIFIGAENSIGFHNPTES
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCHHH
MRVLGDSIAFATKAEAFLRQALAQAGVIVPAKVDLELAKYLNNRGAAKLNFRKELELPDP
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHCCCCCEEEECCEECCCCCC
SGIQNRF
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12949112 [H]