Click here to switch to the map view.

The map label for this gene is nemA [H]

Identifier: 118579539

GI number: 118579539

Start: 1150194

End: 1151258

Strand: Reverse

Name: nemA [H]

Synonym: Ppro_1106

Alternate gene names: 118579539

Gene position: 1151258-1150194 (Counterclockwise)

Preceding gene: 118579542

Following gene: 118579538

Centisome position: 28.72

GC content: 57.28

Gene sequence:

>1065_bases
ATGCCAACATTATTCGATCCCATTACAGTTGGCGCTCTGCGCCTGCCCAACCGAATTATCATGGCGCCCATGACGCGAGC
ACGTGCCTCTGCCGACACTCGTGTTCCCCTGCCAATGATGGCGGAGTATTACTCCCAGCGTGCCACCGCTGGTTTAATTA
TTTCTGAGGCCACTGTGGTTGACCGCATGGGTGTGGGTTATGAAGCAACTCCGGGCATCTGGTCGCACGAACAAGTCGAG
GGATGGAAGTCTGTTACCAAGGCCGTTCATGATGCTGGGGGACGCATCTTCCTTCAGCTTTGGCATGTGGGGCGGATTTC
CGATCCCAGCTTCCTGGGCGGCAAGCCACCAGTCTCGGCCAGTGCCATTGCGGCCAAGGGGCATGTTAGTCTTCTACGTC
CAAAACGACCATATCCAGTGCCGCATGCGCTGGAACTGCACAAAATACCGCAAATCATTGCCGCCTACCGCAATGGTGCG
ATCAATGCAAAAACCGCCTGTTTTGACGGTGTGGAGATCCACGGAGCTAACGGCTATCTGCTAGACCAGTTCCTGCAGGA
TAGTACGAATAGGCGAACGGATGCATACGGTGGCTCACGGGAGAACCGGGCGCGCCTGATGCTTGAGGTTACCGATGCCG
TCGTTGAGGTCTGGGGAGCTGGGCGGGTAGGCATGCATCTCGCTCCGCGAGCGGACTTTCATGACATGGGTGATTCCGAC
CGTCTTGCCACCTTCACGTATGTGGCGCGCGAGCTCGGTAAACGTGGTCTTGCTTTCATCTGCGCCCGCGAGGCACGCCT
CGAAGACAGCATTGGTCCCCTCATACGGGAAGCATTCGGAGGTGCATATATCGCCAACGAGAGCTTCACCCGCGAAACAG
CCGAGCAGGCACTGGCGGATGGAGTTGCCGATGCCGTTGCTTTCGGCAAGGCGTTCATCGCCAATCCCGACCTGGTTGCC
CGGTTCGCCGCGAATGCCCCTTTGAACGCCTGGGATGACAAGACGTTTTACGTCGGCGGTGCCAAGGGCTACATTGATTA
CACGACGTTACCTCATGCTATTTGA

Upstream 100 bases:

>100_bases
GACGACTTATTCAATAAATACAGCAGGTTAACCAATTGAAAACGCCCTGGAGATGACGGCTGAATGCGTTAAGCCCGACA
GACTCCTAGAGGAGGTCCTT

Downstream 100 bases:

>100_bases
AACCACCTCATCAGGGAGGCGCCGGTGGTGTGCGATGTGTTGTCAGAGACCCACACTATTGTATCCTCGAAGTTGATGCC
ATTTTAGCTGCACGCCGGTT

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MPTLFDPITVGALRLPNRIIMAPMTRARASADTRVPLPMMAEYYSQRATAGLIISEATVVDRMGVGYEATPGIWSHEQVE
GWKSVTKAVHDAGGRIFLQLWHVGRISDPSFLGGKPPVSASAIAAKGHVSLLRPKRPYPVPHALELHKIPQIIAAYRNGA
INAKTACFDGVEIHGANGYLLDQFLQDSTNRRTDAYGGSRENRARLMLEVTDAVVEVWGAGRVGMHLAPRADFHDMGDSD
RLATFTYVARELGKRGLAFICAREARLEDSIGPLIREAFGGAYIANESFTRETAEQALADGVADAVAFGKAFIANPDLVA
RFAANAPLNAWDDKTFYVGGAKGYIDYTTLPHAI

Sequences:

>Translated_354_residues
MPTLFDPITVGALRLPNRIIMAPMTRARASADTRVPLPMMAEYYSQRATAGLIISEATVVDRMGVGYEATPGIWSHEQVE
GWKSVTKAVHDAGGRIFLQLWHVGRISDPSFLGGKPPVSASAIAAKGHVSLLRPKRPYPVPHALELHKIPQIIAAYRNGA
INAKTACFDGVEIHGANGYLLDQFLQDSTNRRTDAYGGSRENRARLMLEVTDAVVEVWGAGRVGMHLAPRADFHDMGDSD
RLATFTYVARELGKRGLAFICAREARLEDSIGPLIREAFGGAYIANESFTRETAEQALADGVADAVAFGKAFIANPDLVA
RFAANAPLNAWDDKTFYVGGAKGYIDYTTLPHAI
>Mature_353_residues
PTLFDPITVGALRLPNRIIMAPMTRARASADTRVPLPMMAEYYSQRATAGLIISEATVVDRMGVGYEATPGIWSHEQVEG
WKSVTKAVHDAGGRIFLQLWHVGRISDPSFLGGKPPVSASAIAAKGHVSLLRPKRPYPVPHALELHKIPQIIAAYRNGAI
NAKTACFDGVEIHGANGYLLDQFLQDSTNRRTDAYGGSRENRARLMLEVTDAVVEVWGAGRVGMHLAPRADFHDMGDSDR
LATFTYVARELGKRGLAFICAREARLEDSIGPLIREAFGGAYIANESFTRETAEQALADGVADAVAFGKAFIANPDLVAR
FAANAPLNAWDDKTFYVGGAKGYIDYTTLPHAI

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=360, Percent_Identity=46.1111111111111, Blast_Score=299, Evalue=2e-82,
Organism=Escherichia coli, GI1789463, Length=339, Percent_Identity=30.3834808259587, Blast_Score=120, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17559804, Length=169, Percent_Identity=34.3195266272189, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17565138, Length=295, Percent_Identity=28.8135593220339, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI72001454, Length=168, Percent_Identity=32.7380952380952, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17559802, Length=281, Percent_Identity=28.8256227758007, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17540738, Length=305, Percent_Identity=26.2295081967213, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17564188, Length=213, Percent_Identity=28.6384976525822, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6325086, Length=386, Percent_Identity=37.8238341968912, Blast_Score=221, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6321973, Length=386, Percent_Identity=36.2694300518135, Blast_Score=210, Evalue=4e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 38406; Mature: 38275

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTLFDPITVGALRLPNRIIMAPMTRARASADTRVPLPMMAEYYSQRATAGLIISEATVV
CCCCCCCHHHHHHHCCCEEEECCCHHHHCCCCCCCCHHHHHHHHHHHHHHCEEEEHHHHH
DRMGVGYEATPGIWSHEQVEGWKSVTKAVHDAGGRIFLQLWHVGRISDPSFLGGKPPVSA
HHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCH
SAIAAKGHVSLLRPKRPYPVPHALELHKIPQIIAAYRNGAINAKTACFDGVEIHGANGYL
HHEEECCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCEEEECCCCHH
LDQFLQDSTNRRTDAYGGSRENRARLMLEVTDAVVEVWGAGRVGMHLAPRADFHDMGDSD
HHHHHHCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCC
RLATFTYVARELGKRGLAFICAREARLEDSIGPLIREAFGGAYIANESFTRETAEQALAD
HHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHH
GVADAVAFGKAFIANPDLVARFAANAPLNAWDDKTFYVGGAKGYIDYTTLPHAI
HHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCEEEEECCCCEEEECCCCCCC
>Mature Secondary Structure 
PTLFDPITVGALRLPNRIIMAPMTRARASADTRVPLPMMAEYYSQRATAGLIISEATVV
CCCCCCHHHHHHHCCCEEEECCCHHHHCCCCCCCCHHHHHHHHHHHHHHCEEEEHHHHH
DRMGVGYEATPGIWSHEQVEGWKSVTKAVHDAGGRIFLQLWHVGRISDPSFLGGKPPVSA
HHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCH
SAIAAKGHVSLLRPKRPYPVPHALELHKIPQIIAAYRNGAINAKTACFDGVEIHGANGYL
HHEEECCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCEEEECCCCHH
LDQFLQDSTNRRTDAYGGSRENRARLMLEVTDAVVEVWGAGRVGMHLAPRADFHDMGDSD
HHHHHHCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCC
RLATFTYVARELGKRGLAFICAREARLEDSIGPLIREAFGGAYIANESFTRETAEQALAD
HHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHH
GVADAVAFGKAFIANPDLVARFAANAPLNAWDDKTFYVGGAKGYIDYTTLPHAI
HHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCEEEEECCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]