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The map label for this gene is pulE [H]

Identifier: 118579493

GI number: 118579493

Start: 1109045

End: 1110835

Strand: Direct

Name: pulE [H]

Synonym: Ppro_1060

Alternate gene names: 118579493

Gene position: 1109045-1110835 (Clockwise)

Preceding gene: 118579492

Following gene: 118579496

Centisome position: 27.67

GC content: 61.47

Gene sequence:

>1791_bases
ATGTCCGAAACCAGAGAACTCACCGTAGAAAACATCGCCCACCTCTTGAAGCAGCGCGGCCTCCTCTCGGCCGCGCAGGT
GAAGGACCTCCTGGCGCGGGAAAAAGCCCAGCAAACGCGACTGGTTTCCTTGCACCACCCTGGCGCCCTGCGCCGCGGCC
ATCCCGTGGCGGAGCTGGTTTCTCCCGCCGAGCTGATCGCCTCGTTCAATCTGGAAATCTGCGACGCCCCTGGCTCCTGG
CTGACCGAGGATGTCATCACCGAGGTGGTGGCCGACAAGCTGGGCATCCCCTACCTGAAGATCGACCCGCTCAAACTCAA
CCTGGACGTCGTAACCGCCCACATCCCGCGTCCCTTCGCCCTCAAACACCTGATCGTTACCGTTGCCGAGGAACCGGGGG
CCATCACGGTCGCCGTGGCCAACCCTTTCAACCTGCATCTGGTGGATGAACTGGCTGCCGCGCGGAGATTGGCGATCCGG
CGGGTGCTCAGTTCCAAGAGCGATATCCTCAAGATCCTGCGGGAGTTTTACGGTTTTCGCGCCTCGGTGGTGGCTGCCGA
GGCCGAGGCGGCAAACGGTGTTGACCTGGGCAACCTGGAGCAGTTTGTCAGGCTTAAGGGGCAGGGGGAGATCGAGAGTA
ACGACAAACATGTGGTGGCAGCGGTGGAATTCCTGCTGCAGTACGCCTTTGACCAGCGTGCCAGCGATATCCATATCGAG
CCCAAGCGCGAGAAGTCGTTGGTGCGCTTCCGGCTGGACGGGGTCATGCATAACATCCACAGCATTCCCAAGCAGCTGCA
CGCCCCGATGGTGTCGCGCATCAAGCTTCTCTCCCGCATGGACCTGGCCGAGAAGCGCCGTCCCCAGGATGGCCGCATCA
AGACCAGCGTCAACGCCAAGGAGATGGAACTGCGCGTCTCCACCGTACCGGTGGCCTTTGGGGAAAAGGTGGTCATAAGG
ATCTTCGATCCGGATATCCTGCTGCAGGACCTGGACAGCATGGGCTTTAATCCCCGTGAGCTGACGCTGTACAACTCCTT
CATCAACCGCCCCAACGGCATTATTCTGGTAACCGGCCCCACCGGCAGCGGCAAGACCACCACGCTCTACTCCACCCTGA
GGAAGCTCTCCTCGCCCGAGGTCAATATCGTTACGGTAGAGGACCCGATCGAGATGGTAATGGAGGAGTTCAACCAGATC
GGCGTTCAGCATGCCATCGGCGTGGGGTTCGATACCATCCTGCGCAACATCCTGCGCCAGGACCCGGACGTGATCATGAT
CGGCGAAATCCGCGACCGGGAGACCGCTGAAAACGCCGTGCAGGCCGCTTTGACCGGGCACCTGGTGCTCTCGACCCTGC
ACACCAACGATTCGGTCTCGTCCATCATCCGCCTGCTGGACTTGGGGGTGCCGTCGTTTCTGATCTCGGCCACGGTAATC
GGCATCATCGCCCAGCGCCTGGTGCGCAGGATCTGCCCTCGTTGCAGGAGCGAGCGGGAACTGACTTCTGCCGAACAGGA
GTACCTGCGGCTGGGCAAGAAGGGGCTTACGGTCTGGGAGGGCAAGGGGTGTAAGGAGTGCCGCGGTACCGGGTACAAGG
GGCGCACCGGACTGTTCGAGGTGCTGGATATGAGCGACCGTATCAAGGGACTGATCAGCGCCGACGTGGAACTGGCCGAC
CTGCTGGCTGCGGCTGGCGAGGATGGCCTGATAACCCTGCGCCAGGTGGCGGTGCGCAAGATGCTGGAAGGGATCACGAC
CTATGAGGAAGTCATCGCCATGACAGGTTAA

Upstream 100 bases:

>100_bases
CCCGAACAGGTGATCGAAGAGGTCGCCAAGCGCATCGAGTCGCAGAAGGCCAATGCCGCCAAGCGTGCCGTGAAGTAGCG
CCGGTCCTGCCGAAGGAACC

Downstream 100 bases:

>100_bases
GCGCTCACCGACCTGCGTGACGCACAAAAAAACCGCCCGGGAATCAGGCGGTTTTTTTGTGTGGAGAATCTTCGCGATGT
CAGCAGGCGGAGAGCCGGCG

Product: type II secretion system protein E

Products: NA

Alternate protein names: Pullulanase secretion protein pulE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 596; Mature: 595

Protein sequence:

>596_residues
MSETRELTVENIAHLLKQRGLLSAAQVKDLLAREKAQQTRLVSLHHPGALRRGHPVAELVSPAELIASFNLEICDAPGSW
LTEDVITEVVADKLGIPYLKIDPLKLNLDVVTAHIPRPFALKHLIVTVAEEPGAITVAVANPFNLHLVDELAAARRLAIR
RVLSSKSDILKILREFYGFRASVVAAEAEAANGVDLGNLEQFVRLKGQGEIESNDKHVVAAVEFLLQYAFDQRASDIHIE
PKREKSLVRFRLDGVMHNIHSIPKQLHAPMVSRIKLLSRMDLAEKRRPQDGRIKTSVNAKEMELRVSTVPVAFGEKVVIR
IFDPDILLQDLDSMGFNPRELTLYNSFINRPNGIILVTGPTGSGKTTTLYSTLRKLSSPEVNIVTVEDPIEMVMEEFNQI
GVQHAIGVGFDTILRNILRQDPDVIMIGEIRDRETAENAVQAALTGHLVLSTLHTNDSVSSIIRLLDLGVPSFLISATVI
GIIAQRLVRRICPRCRSERELTSAEQEYLRLGKKGLTVWEGKGCKECRGTGYKGRTGLFEVLDMSDRIKGLISADVELAD
LLAAAGEDGLITLRQVAVRKMLEGITTYEEVIAMTG

Sequences:

>Translated_596_residues
MSETRELTVENIAHLLKQRGLLSAAQVKDLLAREKAQQTRLVSLHHPGALRRGHPVAELVSPAELIASFNLEICDAPGSW
LTEDVITEVVADKLGIPYLKIDPLKLNLDVVTAHIPRPFALKHLIVTVAEEPGAITVAVANPFNLHLVDELAAARRLAIR
RVLSSKSDILKILREFYGFRASVVAAEAEAANGVDLGNLEQFVRLKGQGEIESNDKHVVAAVEFLLQYAFDQRASDIHIE
PKREKSLVRFRLDGVMHNIHSIPKQLHAPMVSRIKLLSRMDLAEKRRPQDGRIKTSVNAKEMELRVSTVPVAFGEKVVIR
IFDPDILLQDLDSMGFNPRELTLYNSFINRPNGIILVTGPTGSGKTTTLYSTLRKLSSPEVNIVTVEDPIEMVMEEFNQI
GVQHAIGVGFDTILRNILRQDPDVIMIGEIRDRETAENAVQAALTGHLVLSTLHTNDSVSSIIRLLDLGVPSFLISATVI
GIIAQRLVRRICPRCRSERELTSAEQEYLRLGKKGLTVWEGKGCKECRGTGYKGRTGLFEVLDMSDRIKGLISADVELAD
LLAAAGEDGLITLRQVAVRKMLEGITTYEEVIAMTG
>Mature_595_residues
SETRELTVENIAHLLKQRGLLSAAQVKDLLAREKAQQTRLVSLHHPGALRRGHPVAELVSPAELIASFNLEICDAPGSWL
TEDVITEVVADKLGIPYLKIDPLKLNLDVVTAHIPRPFALKHLIVTVAEEPGAITVAVANPFNLHLVDELAAARRLAIRR
VLSSKSDILKILREFYGFRASVVAAEAEAANGVDLGNLEQFVRLKGQGEIESNDKHVVAAVEFLLQYAFDQRASDIHIEP
KREKSLVRFRLDGVMHNIHSIPKQLHAPMVSRIKLLSRMDLAEKRRPQDGRIKTSVNAKEMELRVSTVPVAFGEKVVIRI
FDPDILLQDLDSMGFNPRELTLYNSFINRPNGIILVTGPTGSGKTTTLYSTLRKLSSPEVNIVTVEDPIEMVMEEFNQIG
VQHAIGVGFDTILRNILRQDPDVIMIGEIRDRETAENAVQAALTGHLVLSTLHTNDSVSSIIRLLDLGVPSFLISATVIG
IIAQRLVRRICPRCRSERELTSAEQEYLRLGKKGLTVWEGKGCKECRGTGYKGRTGLFEVLDMSDRIKGLISADVELADL
LAAAGEDGLITLRQVAVRKMLEGITTYEEVIAMTG

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of pullulanase [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1786296, Length=373, Percent_Identity=42.3592493297587, Blast_Score=313, Evalue=2e-86,
Organism=Escherichia coli, GI1789723, Length=384, Percent_Identity=42.4479166666667, Blast_Score=311, Evalue=1e-85,
Organism=Escherichia coli, GI87082188, Length=184, Percent_Identity=38.0434782608696, Blast_Score=103, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 65871; Mature: 65740

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETRELTVENIAHLLKQRGLLSAAQVKDLLAREKAQQTRLVSLHHPGALRRGHPVAELV
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHC
SPAELIASFNLEICDAPGSWLTEDVITEVVADKLGIPYLKIDPLKLNLDVVTAHIPRPFA
CHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHCCCEEEECCEEEEEEEEEECCCCCHH
LKHLIVTVAEEPGAITVAVANPFNLHLVDELAAARRLAIRRVLSSKSDILKILREFYGFR
HHHHHHEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCH
ASVVAAEAEAANGVDLGNLEQFVRLKGQGEIESNDKHVVAAVEFLLQYAFDQRASDIHIE
HHEEEEHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
PKREKSLVRFRLDGVMHNIHSIPKQLHAPMVSRIKLLSRMDLAEKRRPQDGRIKTSVNAK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHH
EMELRVSTVPVAFGEKVVIRIFDPDILLQDLDSMGFNPRELTLYNSFINRPNGIILVTGP
HEEEEEEEECEECCCEEEEEEECCHHHHHHHHHCCCCCCEEEEEHHHHCCCCCEEEEECC
TGSGKTTTLYSTLRKLSSPEVNIVTVEDPIEMVMEEFNQIGVQHAIGVGFDTILRNILRQ
CCCCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHCHHHHHCCCHHHHHHHHHHC
DPDVIMIGEIRDRETAENAVQAALTGHLVLSTLHTNDSVSSIIRLLDLGVPSFLISATVI
CCCEEEECCCCCHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHCCCHHHHHHHHHH
GIIAQRLVRRICPRCRSERELTSAEQEYLRLGKKGLTVWEGKGCKECRGTGYKGRTGLFE
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEECCCCCHHHCCCCCCCCCCHHH
VLDMSDRIKGLISADVELADLLAAAGEDGLITLRQVAVRKMLEGITTYEEVIAMTG
HHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SETRELTVENIAHLLKQRGLLSAAQVKDLLAREKAQQTRLVSLHHPGALRRGHPVAELV
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHC
SPAELIASFNLEICDAPGSWLTEDVITEVVADKLGIPYLKIDPLKLNLDVVTAHIPRPFA
CHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHCCCEEEECCEEEEEEEEEECCCCCHH
LKHLIVTVAEEPGAITVAVANPFNLHLVDELAAARRLAIRRVLSSKSDILKILREFYGFR
HHHHHHEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCH
ASVVAAEAEAANGVDLGNLEQFVRLKGQGEIESNDKHVVAAVEFLLQYAFDQRASDIHIE
HHEEEEHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
PKREKSLVRFRLDGVMHNIHSIPKQLHAPMVSRIKLLSRMDLAEKRRPQDGRIKTSVNAK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHH
EMELRVSTVPVAFGEKVVIRIFDPDILLQDLDSMGFNPRELTLYNSFINRPNGIILVTGP
HEEEEEEEECEECCCEEEEEEECCHHHHHHHHHCCCCCCEEEEEHHHHCCCCCEEEEECC
TGSGKTTTLYSTLRKLSSPEVNIVTVEDPIEMVMEEFNQIGVQHAIGVGFDTILRNILRQ
CCCCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHCHHHHHCCCHHHHHHHHHHC
DPDVIMIGEIRDRETAENAVQAALTGHLVLSTLHTNDSVSSIIRLLDLGVPSFLISATVI
CCCEEEECCCCCHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHCCCHHHHHHHHHH
GIIAQRLVRRICPRCRSERELTSAEQEYLRLGKKGLTVWEGKGCKECRGTGYKGRTGLFE
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEECCCCCHHHCCCCCCCCCCHHH
VLDMSDRIKGLISADVELADLLAAAGEDGLITLRQVAVRKMLEGITTYEEVIAMTG
HHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1738317; 2677007 [H]